Endo--galactosidase from Bacteroides fragilis

Enzyme Commission (EC) Number:	3.2.1.103 ( BRENDA | IUBMB )
MDL number:	MFCD00131433
NACRES:	NA.54

Кат. номер

G6920-.5UN-PW

G6920-.5UN

Endo-β-galactosidase was used in fractional protein isolation. It was used for deglycosylation in glycoproteomics of the endothelial secretome of human endothelial cells.
Packaging
0.5 unit in glass insert
Biochem/physiol Actions
Internal (1-4) galactose linkages in unbranched, repeating poly-Nacetyllactosamine [GlcNAc (1-3)Gal (1-4)] structures are the preferred substrate.
Sulfated structures such as keratan sulfate are also cleaved. Branching and/or fucosylation of the substrate may reduce or completely inhibit cleavage. Sulfation of C-6 on galactose will block cleavage. Oligosaccharides of the neolacto-group are cleaved at greatly reduced rates depending on the deviation from the preferred substrate. For example, Gal (1-3)GlcNAc (1-3) Gal (1-4)Glc is cleaved at 5X10-5 the rate of keratan sulfate
-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of -galactosidase, in a feedback loop.
Unit Definition
One unit will release 1.0 μmole of reducing sugar from bovine corneal keratan sulfate per minute at 37 °C, pH 5.8.
Physical form
Aseptically filled solution in 20 mM Tris-HCl, pH 7.5

Quality Level	100,
recombinant	expressed in E. coli
sterility	aseptically filled
form	buffered aqueous solution
specific activity	140 units/mg protein
mol wt	32 kDa
storage temp.	2-8°C

Personal Protective Equipment	Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable