Chondroitinase AC from Flavobacterium heparinum

CAS Number:	9047-57-8
Enzyme Commission (EC) Number:	4.2.2.5 ( BRENDA | IUBMB )
MDL number:	MFCD00130796
NACRES:	NA.54

Кат. номер

E2039-10UN

E2039-10UN-PW

Chondroitinase AC has been applied to the analysis of chondroitin sulfate in commercial samples such as dietary supplements.
Chondroitinase AC from Flavobacterium heparinum is an enzyme that cleaves sulfated and non-sulfated polysaccharide chains with (1-4) linkages between hexosamines and glucuronic acid residues, by an elimination mechanism. The resulting oligosaccharide products are mainly disaccharides with unsaturated uronic acids. Chondroitinase AC specifically degrades chondroitin sulfates A and C, but not chondroitin sulfate B (dermatan sulfate).
Highly purified to remove interferring β-glucuronidase and protease activities for use in the hydrolysis of chondroitin sulfate pror to HPLC analysis. β-Glucuronidase activity: ≤2,000 femtomole/min/mg protein, Protease activity: ≤2,000 femtomole/min/mg protein
1 unit is defined as the amount of enzyme that will liberate 1.0 µmole per minute of unsaturated disaccharides from chondroitin sulfate A at pH 6.7 at 37 °C as measured by the change in A232. The µ for the reaction product -Di-4S (chondroitin sulfates A and B) is 5.1 and 5.5 for -Di-6S (chondroitin sulfate C).

Quality Level	200
recombinant	expressed in E. coli
assay	90% (SDS-PAGE)
form	lyophilized solid
specific activity	200 units/mg protein
shipped in	dry ice
storage temp.	20°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable