GenElute™ Plant Genomic DNA Miniprep Kit G2N350-1KT

NACRES:

NA.55

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
The GenElute Plant Genomic DNA Miniprep Kit provides a simple and convenient way to isolate pure DNA from a variety of plant species. The GenElute kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, RNase treatment, and hazardous organic compounds such as phenol and chloroform.
The purified genomic DNA is ready for immediate use in sensitive downstream applications such as:

• PCR
• restriction endonuclease digestion
• cloning
• Southern blots

Features and Benefits

• Starting material: Up to 100 mg of plant tissue
• Expected yield: Up to 20 µg
• Elution volume: 100 - 200 µl
• Time required: < 40 min
• RNase treatment required: No

Principle
Several micrograms of DNA can be obtained from up to 100 mg of fresh tissue or 10 mg of freeze-dried material in less than an hour. Plant tissue is disrupted by grinding in liquid nitrogen and the DNA is released with detergent and chaotrope. Proteins, polysaccharides, and cell debris are eliminated with a 10 minute precipitation procedure followed by centrifugation through a filtration column, included in the kit. The genomic DNA is purified further by a silica bind-wash-elute procedure in microcentrifuge spin columns. The purified DNA is greater than 20 kb in length.
GenElute is a trademark of Sigma-Aldrich Co. LLC

Quality Level	200,
usage	sufficient for 350 purifications
greener alternative product characteristics	Inherently Safer Chemistry for Accident Prevention Learn more about the Principles of Green Chemistry,.
storage temp.	room temp

pictograms	GHS05,GHS07
signalword	Danger
hcodes	H290 - H302 + H332 - H314 - H412
pcodes	P273 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
Supp Hazards	EUH032
RIDADR	UN 3316 9