- Производитель:
- Roche
Sample material:
Human whole blood (treated with sodium heparin, EDTA or sodium citrate): 1 to 10 mL
Isolated lymphocytes: 1 to 10 mL
Isolated buffy coat: buffy coat from 10 to 20 mL whole blood, containing at least 1.0 x 107 leukocytes.
Purity of isolated DNA: Average A 260 / A 280 ratio: 1.7 - 1.9
The DNA Isolation Kit for Mammalian Blood rapidly isolates DNA from 1 to 10 ml mammalian whole blood, lymphocytes, or buffy coat samples.
The DNA Isolation Kit for Mammalian Blood purifies DNA that is suitable for most molecular biology applications:
- PCR/long PCR
- Sequencing
- Restriction digestion
- Southern blotting
- Cloning
- Process multiple samples simultaneously.
- Use a cost-effective kit.
- Obtain consistent and reliable results.
- Improve safety.
- Red Blood Cell Lysis Buffer
- White Blood Cell Lysis Buffer
- Protein Precipitation Solution
Figure 2: Southern blot analysis of DNA from various human blood samples prepared with the DNA Isolation Kit for Mammalian Blood. DNA was prepared from several research samples, including human blood containing different anticoagulants and lymphocyte and buffy coat preparations. Ten µg of each preparation was digested with Eco RI, separated by gel electrophoresis, and transferred to a nylon membrane by Southern blotting. The n-ras gene in each sample was detected with a DIG-labeled n-ras probe.
Lanes 2, 3: Blood sample containing sodium citrate
Lanes 4, 5: Blood sample containing heparin
Lanes 6, 7: Blood sample containing sodium EDTA
Lanes 8, 9: Buffy coat preparation
Lanes 10, 11: Lymphocyte preparation
Lanes 1, 12: DNA Molecular Weight Marker VII
Result: Each lane contained only a single hybridized band of the expected size.
Figure 1: Amplification of large DNA fragments from mammalian blood prepared with the DNA Isolation Kit for Mammalian Blood. DNA was prepared from two research samples, one containing 10 ml human blood, the other containing 10 ml mouse blood. Aliquots of each DNA preparation were used as templates for the amplification of several gene fragments with the Expand Long Template PCR System. PCR products were analyzed by gel electrophoresis.
Left panel: Gene fragments amplified from human samples
Lanes 2, 3: tPA fragment (9.3 kb) amplified from 25 ng DNA
Lanes 4, 5: tPA fragment (15 kb) amplified from 50 ng DNA
Lanes 6, 7: -globin fragment (23 kb) amplified from 100 ng DNA
Lanes 8, 9: -globin fragment (28 kb) amplified from 200 ng DNA
Lanes 1, 10: DNA Molecular Weight Marker III
Right panel: Gene fragments amplified from mouse samples
Lanes 2, 3: IL-2 gene (4.2 kb) amplified from 330 ng DNA
Lanes 4, 5: -2 collagen fragment (5.6 kb) amplified from 100 ng DNA
Lanes 6, 7: -2 collagen fragment (10.4 kb) amplified from 50 ng DNA
Lanes 8, 9: -2 collagen fragment (15.4 kb) amplified from 100 ng DNA
Lanes 1, 10: DNA Molecular Weight Marker III
Quality Level | 100 |
Торговая марка | Roche |
packaging | kit of for 25 reactions |
pictograms | GHS07 |
signalword | Warning |
hcodes | H319 |
pcodes | P264 - P280 - P305 + P351 + P338 - P337 + P313 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point F | does not flash |
Flash Point C | does not flash |
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