Imprint® RNA Immunoprecipitation Kit RIP-12RXN

NACRES:

NA.54

RNA immunoprecipitation (RIP) is a very powerful procedure for the study of RNA binding proteins (RBPs) and their RNA targets in ribonucleoprotein (RNP) complexes. Antibodies raised against specific RBPs are used to coprecipitate RNPs, i.e., the RBP along with its RNA partner. The RNA can then be identified by next generation sequencing, or if testing for a specific RNA, by RT-PCR.
Our Imprint RNA Immunoprecipitation Kit provides all reagents needed for successful RIP when paired with an appropriate antibody. The kit includes two lysis buffers, one mild for less tightly bound RNPs, the other harsh for strongly associated RNPs. Protein A magnetic beads are provided to collect immune precipitates and facilitate washing. The kit also includes both rabbit and mouse IgG for negative control RIPs, a rabbit anti-mouse bridging antibody to bind mouse monoclonal primary antibodies efficiently to protein A on the magnetic beads, as well as both protease and ribonuclease inhibitors to protect the RNPs from degradation during RIP.
Learn more about this product and view workflow and application data.
Imprint® RNA Immunoprecipitation Kit has been used for:

• Suitable for downstream applications
• Individual target characterization to genome-wide profiling techniques
• Characterization of signal transduction pathways
• Verification of ChIp-chIP and ChIP-seq data

• Xtra Protein A magnetic beads with higher binding capacity
• Step-by-step protocols for cell lysis and immunoprecipitation
• RNAse inhibitors and RNAse-free reagents to maintain RNA integrity
• Negative control and antibody included

Imprint is a registered trademark of Sigma-Aldrich Co. LLC

Quality Level

200

pictograms	GHS02,GHS07
signalword	Warning
hcodes	H226 - H315 - H319 - H335
pcodes	P261 - P305 + P351 + P338
RIDADR	UN 3316 9
Flash Point F	100.4 °F
Flash Point C	38 °C