Core Histone Isolation Kit EPI024-1KT

NACRES:

NA.25

Histones are small alkaline proteins that interact with eukaryotic DNA to form the fundamental subunit of chromatin known as the nucleosome. The core histones H2A, H2B, H3 and H4 form the central spooling unit of the nucleosome; with two copies of each comprising a histone octamer that interacts with the negatively charged DNA. They enable an ordered compaction of the DNA, as well as spatiotemporal control of gene expression and genomic replication throughout mitosis. Post-translational modification of this core complex results in structural changes within the chromatin and epigenetic control of gene expression.
Suitable for the isolation of intact functional core histones from tissue and cultured cells, protein profiling, post-translational modification and epigenetic analyses and Western blot and ELISA
The Core Histone Isolation Kit offers an optimized suite of reagents to rapidly extract the total core histones, while preserving acetylation from tissues and cultured cells for biochemical and epigenetic analyses. One hundred standard extractions at a scale of 107 cells or 100 mg of tissue can be performed with the reagents in this kit. Yield of the total histone proteins is ~0.4 mg per 107 cells or 100 mg of tissue.

usage	sufficient for 100 extractions
storage temp.	−20°C

pictograms	GHS05
signalword	Danger
hcodes	H290 - H315 - H318 - H412
pcodes	P234 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
RIDADR	UN 3316 9