Adenosine Deaminase Activity Assay Kit (Fluorometric) EPI020-1KT

NACRES:

NA.25

Adenosine Deaminase (ADA) (E.C. 3.5.4.4.) is an enzyme that catalyzes the conversion of adenosine and 2-deoxyadenosine to inosine and 2-deoxyinosine. Adenosine Deaminase is widely distributed in various tissues and cells. There are two isoforms, ADA1 and ADA2. ADA1 is widely expressed in most cells in the body, particularly in lymphocytes and macrophages. It is present in the cytosol, nucleus and found associated with dipeptidyl peptidase-4 on the cell membrane. ADA2 was first found in the spleen but is predominantly found in the plasma and serum. Increased serum ADA levels are found in certain infectious diseases such as tuberculosis and various liver diseases such as acute hepatitis, alcoholic hepatic fibrosis, chronic active hepatitis to name a few. Adenosine Deaminase is also a marker for T-lymphocyte proliferation.
Suitability
Suitable for the detection of Adenosine Deaminase activity in cell and tissue lysate, nuclear extract, cell medium and purified recombinant protein
Principle
Inosine formed from the breakdown of adenosine is detected via a multi-step reaction, resulting in the formation of an intermediate that reacts with the ADA Probe to generate a fluorescent product. The kit measures total Activity of Adenosine Deaminase with limit of quantification of 10 μU of recombinant adenosine deaminase. The range of the assay is from 10–100 μU.

usage	sufficient for 100 fluorometric tests
detection method	fluorometric
storage temp.	−20°C

pictograms	GHS08
signalword	Danger
hcodes	H319 - H334
pcodes	P261 - P264 - P280 - P284 - P305 + P351 + P338 - P337 + P313
RIDADR	NONH for all modes of transport