Anti-Zfp64 Antibody, clone 3B22 ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1199-4X25UL

ZRB1199-25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 3B22 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Zinc finger protein 64 (Zfp64). It targets an epitope within 21 amino acids from the N-terminal half.
KLH-conjugated linear peptide corresponding to 21 amino acids from the N-terminal half of human Zinc finger protein 64 (Zfp64).
Anti-Zfp64, clone 3B22 ZooMAb®, Cat. No. ZRB1199, is a rabbit recombinant monoclonal antibody that specifically targets Zfp64 and is tested for use in Affinity Binding Assay, Immunocytochemistry, and Western Blotting.
Quality Control Testing
Evaluated by Western Blotting in Jurkat cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Zfp64 in Jurkat cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Zfp64 in A549 cell lysate.
Affinity Binding Assay: A representative lot of this antibody bound Zfp64 peptides with a KD of 1.1 x 10-8 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Zfp64 in Jurkat cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Zinc finger protein 64 (UniProt: Q9NPA5; also known as Zfp-64, Zinc finger protein 338) is encoded by the ZFP64 (also known as ZNF338) gene (Gene ID: 55734) in human. ZNF64 is a member of the krueppel C2H2 type zinc-finger protein family. It is a transcriptional regulatory protein with 13 zinc finger regions. ZFP64 has been identified as an essential transcription factor in MLL-rearranged leukemia where it maintains MLL expression via binding to the MLL promoter, which is the most enriched location of ZFP64. ZFP64 expression is reported to be under the control of Runx2, and it is upregulated by direct transactivation of its promoter. It is shown to associate with Notch 1 and suppress the myogenic differentiation of C2C12 cells and promotes their osteoblastic differentiation. ZFP64 is shown to promote Toll-like receptor-triggered proinflammatory and type I interferon production in macrophages by enhancing the activation of p65 subunit of NF-kB. ZFP64 can bind to the promoter of the TNF- , IL-6, and IFN- genes in macrophages following TLR ligation. Upon stimulation of cells with lipopolysaccharide (LPS), ZFP64 associates with p65 subunit of NF-kB. Six different isoforms of ZFP64 have been described that are produced by alternative splicing. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Lu, B., et al. (2018). Cancer Cell. 34(6): 970-981; Wang, C., et al. (2013). J. Biol. Chem. 288(34); 24600-24608; Sakamoto, K., et al. (2008). J. Cell Sci. 121(10); 1613-1623).
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200,
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	3B22, monoclonal, recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
mol wt	calculated mol wt ~ 72.22 kDa, observed mol wt ~ 80 kDa
species reactivity	human
species reactivity (predicted by homology)	monkey
packaging	antibody small pack of 25 µL
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	affinity binding assay: suitable, immunocytochemistry: suitable, western blot: suitable
isotype	IgG
conjugate	unconjugated
shipped in	ambient
storage temp.	2-8°C

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable