Anti--Tubulin Antibody, clone DM1A ZooMAb® Mouse Monoclonal

Кат. номер

ZMS1039-4X25UL

ZMS1039-25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Specificity
Clone DM1A is a ZooMAb® mouse recombinant monoclonal antibody that detects a-Tubulin in multiple species. It targets an epitope within 24 amino acids from the C-terminal region.
Full-length a-Tubulin from human brain.
Anti-a-Tubulin, clone DM1A ZooMAb®, Cat. No. ZMS1039, is a recombinant Mouse monoclonal antibody that specifically targets a-Tubulin and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Quality Control Testing
Evaluated by Western Blotting in MCF-7 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected a-Tubulin in MCF-7 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected a-Tubulin in NIH3T3 and PC12 cell lysates.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected a-Tubulin in human kidney and human testis tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected a-Tubulin in HeLa, A431, HepG2, and NIH 3T3 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Tubulin alpha-1A chain (UniProt: Q71U36; also known as Alpha-tubulin 3, Tubulin B-alpha-1, Tubulin alpha-3 chain) is encoded by the TUBA1A (also known as TUBA3) gene (Gene ID: 7846) in human. Tubulin is the major constituent of microtubules (MTs). MTs are cytoskeletal filaments composed of a /-tubulin heterodimers. Tubulin dimers constantly polymerize and depolymerize, and MTs can undergo rapid cycles of assembly and disassembly. In the presence of Mg2+ and GTP, a and tubulins join in an end-to-end manner to form protofilaments with alternating a and subunits. Tubulin binds two moles of GTP, one at an exchangeable site on the a-chain and one at a non-exchangeable site on the -chain. GTP bound to a-tubulin is hydrolyzed to GDP during or immediately after polymerization. This weakens the binding affinity of tubulin for adjacent molecules and favors depolymerization that contributes to the dynamic behavior of MTs. MTs also undergo treadmilling, in which tubulin molecules bound to GDP are continually lost from the minus end and are replaced by the addition of GTP-bound tubulin molecules to the plus end of the same MT. Various post-translational modifications, including acetylation, polyglutamylation, polyglycylation, detyrosination, phosphorylation, and palmitoylation, can regulate the polymerization properties of tubulins and/or their interactions with microtubule associated proteins and motor proteins. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200,
biological source	mouse
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	DM1A, monoclonal, recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
mol wt	calculated mol wt ~ 50.14 kDa, observed mol wt ~48 kDa
species reactivity	mouse, human, rat
species reactivity (predicted by homology)	avian, bovine, guinea pig, porcine
packaging	antibody small pack of 25 µL
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
isotype	IgG1
conjugate	unconjugated
shipped in	ambient
storage temp.	2-8°C

WGK Germany

WGK 1