Anti--Tubulin Antibody, clone 1C9 ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1124-25UL

ZRB1124-4X25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 1C9 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects beta-Tubulin. It targets an epitope within 22 amino acids from the C-terminal half.
KLH-conjugated linear peptide corresponding to 22 amino acids from the C-terminal half of human β-Tubulin.
Anti--Tubulin, clone 1C9 ZooMAb®, Cat. No. ZRB1124, is a recombinant Rabbit monoclonal antibody that specifically targets -Tubulin and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blot
Quality Control Testing
Evaluated by Western Blotting in Raji cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected -Tubulin in Raji cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected -Tubulin in Jurkat cell lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected -Tubulin in human tonsil tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound -Tubulin peptide with a KD of 2.5 x 10-8 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected -Tubulin in A431 and HepG2 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Tubulin beta-1 chain (UniProt: Q9H4B7) is encoded by the TUBB1 gene (Gene ID: 81027) in human. Tubulin is one of several members of a small family of globular proteins, which polymerize to form microtubules. The most common members of the tubulin family are alpha-tubulin and beta-tubulin that assemble onto microtubules forming large polymers. During polymerization the /-tubulin heterodimers arrange into linear protofilaments that associate laterally to form the hollow microtubule cylinders. Within a protofilament, the tubulin heterodimers associate in a head-to-tail fashion, which makes microtubules intrinsically polar. The -tubulin within the dimer is oriented toward the plus end and the -tubulin subunit toward the minus end. Tubulin binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. GTP-binding site of beta-tubulin is localized to amino acids 140-146. Multiple isoforms of Tubulin have been described in mammalian tissues. 1 and 4 are reported to be present throughout the cytosol, 2 is localized in the nuclei and nucleoplasm, and 3 is a neuron-specific cytoskeletal protein. 1-tubulin is the most divergent at the amino acid sequence level. It is expressed exclusively in megakaryocytes and platelets in humans and plays an important role in the formation of platelets. It appears late in differentiation and localizes to microtubule shafts and coils within proplatelets. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Lecine, P., et al. (2000). Blood. 96(4):1366-73).
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200,
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	1C9, monoclonal, recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
mol wt	calculated mol wt ~ 50.31 kDa, observed mol wt ~ 55 kDa
species reactivity	human
species reactivity (predicted by homology)	monkey
packaging	antibody small pack of 25 µL
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	affinity binding assay: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
isotype	IgG
conjugate	unconjugated
shipped in	ambient
storage temp.	2-8°C

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable