Anti-CXCR4 Antibody, clone 2L2, ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1357-4X25UL

ZRB1357-25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.,
Specificity
Clone 2L2 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human C-X-C chemokine receptor 4 (CXCR4). It targets an epitope within 12 amino acids from the N-terminal region.
KLH-conjugated linear peptide corresponding to 12 amino acids from the N-terminal region of human C-X-C chemokine receptor type 4 (CXCR4).
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CXCR4 in human adrenal gland tissue sections.
ELISA Analysis: A representative lot specifically detected CXCR4 peptide in ELISA application, but not peptides from five other proteins.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CXCR4 in Jurkat and K562 cell lines.
Affinity Binding Assay: A representative lot of this antibody bound CXCR4 with a KD of 3.6 x 10-10 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Anti-CXCR4, clone 2L2 ZooMAb, Cat. No. ZRB1357, a recombinant Rabbit monoclonal antibody that targets human CXCR4 and is tested in Affinity Binding Assay, ELISA, Immunocytochemistry, and Immunohistochemistry (Paraffin).
Target description
C-X-C chemokine receptor type 4 (UniProt: P61073; also known as CXC-R4, CXCR-4, FB22, Fusin, HM89, LCR1, Leukocyte-derived seven transmembrane domain receptor, LESTR, Lipopolysaccharide-associated protein 3, LAP-3, LPS-associated protein 3, NPYRL, Stromal cell-derived factor 1 receptor, SDF-1 receptor, CD184) is encoded by the CXCR4 gene (Gene ID: 7852) in human. CXCR-4 is a receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium levels and enhancing MAPK1/MAPK3 activation. CXCR-4 is expressed in numerous tissues and two isoforms have been reported that are generated by alternative splicing. Isoform 1 is the predominant form in all tissues tested. CXCR-4 is rapidly phosphorylated on serine and threonine residues in the C-terminal region upon agonist stimulation. Phosphorylation at serine 324 and serine 325 leads to recruitment of ITCH (E3 ubiquitin-protein ligase), ubiquitination and protein degradation. Sulfation on tyrosine 21 is shown to be essential for efficient binding of CXCL12/SDF-1alpha and it promotes its dimerization. Tyrosine 7 and tyrosine 12 are sulfated in a sequential manner following complete sulfation of tyrosine 21. CXCR-4 acts as a co-receptor for human HIV-1 virus isolates (CD4 being the primary receptor) and as a primary receptor for HIV-2 isolates. CXCR-4 can also bind bacterial lipopolysaccharide (LPS) and mediate LPS-induced inflammatory response, including TNF-alpha secretion by monocytes. CXCR-4 is reported to be essential for metastatic spread to organs where CXCL12 is expressed, which allows tumor cells to access cellular niches that favor tumor-cell survival and growth. Mutations in CXCR4 gene are reported to cause WHIM syndrome that is characterized by neutropenia, hypogammaglobulinemia and extensive human papillomavirus (HPV) infection. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Burger, JA., and Kipps, TJ (2006). Blood 107(5);1761-1767).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit (recombinant)
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	2L2, monoclonal, recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
mol wt	calculated mol wt ~39.75 kDa
species reactivity	human
packaging	pkg of 25 µL
greener alternative product characteristics	Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry,.
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	ELISA: suitable, affinity binding assay: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
isotype	IgG
conjugate	unconjugated
greener alternative category	Aligned,
shipped in	ambient
storage temp.	2-8°C

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable