Anti-CD73 (NT5E) Antibody, clone 1F22 ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1035-4X25UL

ZRB1035-25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.,
Specificity
Clone 1F22 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects 5′-Nucleotidase (CD73). It targets an epitope within the N-terminal half.
His-tagged recombinant fragment corresponding to 244 amino acids from the N-terminal half of mature human CD73.
Anti-CD73 (NT5E), clone 1F22 ZooMAb, Cat. No. ZRB1035, is a recombinant Rabbit monoclonal antibody that detects CD73 (NT5E) and is tested for use in Affinity Binding Assay, Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CD73 (NT5E) in A375 cells and in rat spleen and mouse spleen tissue lysates.
Affinity Binding Assay: A representative lot of this antibody bound recombinant NT5E/CD73 protein with a KD of 4.2 x 10-8 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected CD73 (NT5E) in human tonsil tissue sections.
Flow Cytometry Analysis: 0.1 µg from a representative lot detected CD73 (NT5E) in one million HT29 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
5′-Nucleotidase (UniProt: P21589; also known as EC:3.1.3.5, 5′-NT, Ecto-5′-nucleotidase, CD73) is encoded by the NT5E (also known as NT5, NTE) gene (Gene ID: 4907) in human. CD73 is a GPI-anchor membrane glycoprotein that is expressed in most tissues and is involved in the control of a variety of physiological responses including epithelial ion and fluid transport, ischemic preconditioning, tissue injury, platelet function, hypoxia and vascular leak. It is also widely expressed on several tumor tissues and its higher expression levels are associated with tumor neovascularization, invasiveness, and metastasis. Its biological actions are mainly due to its regulated enzymatic phosphohydrolytic activity on extracellular nucleotides. It hydrolyzes extracellular nucleotides into membrane permeable nucleosides and displays AMP-, NAD-, and NMN-nucleosidase activities. CD73 is synthesized with a signal peptide (aa 1-26) and a C-terminal propeptide (aa 550-574) that are subsequently cleaved off to generate the mature form (aa 27-549). Its substrate binding site is localized to amino acids 500-506. Mutations in NT5E gene have been linked to adult-onset of calcification of joints and arteries. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Spychala, J. (2000). Pharmacol. Ther. 87 (2-3); 161-173; Zhang, B. (2010). Cancer Res. 70(16); 6407-6411).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200,
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	1F22, monoclonal, recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
mol wt	calculated mol wt 63.37 kDa, observed mol wt ~63 kDa
species reactivity	human, mouse, rat
packaging	antibody small pack of 25 µL
greener alternative product characteristics	Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry,.
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	affinity binding assay: suitable, flow cytometry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
isotype	IgG
conjugate	unconjugated
greener alternative category	Aligned,
shipped in	ambient
storage temp.	2-8°C

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable