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Anti-CD28 Antibody, clone 2N15 ZooMAb® Rabbit Monoclonal
Гарантия 1 год
- Производитель:
- Sigma-Aldrich
- Производитель:
- Sigma-Aldrich
| Кат. номер |
| ZRB1298-25UL |
| ZRB1298-4X25UL |
General description
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.Specificity
Clone 2N15 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects CD28. It targets an epitope within 15 amino acids from the extracellular domain.
Immunogen
KLH-conjugated linear peptide corresponding to 15 amino acids from the extracellular domain of human CD28.Application
Anti-CD28, clone 2N15 ZooMAb®, Cat. No. ZRB1298, is a recombinant Rabbit monoclonal antibody that detects CD28 and is tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.Quality Control Testing
Evaluated by Western Blotting in Human tonsil tissue lsyate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected CD28 in Human tonsil tissue lysate.
Tested applications
Immunocytochemistry Analysis: A 1:40 dilution from a representative lot detected CD28 in Jurkat cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CD28 in human tonsil tissue sections.
Flow Cytometry Analysis: 1 µg from a representative lot detected CD28 in one million human peripheral blood mononuclear cells (PBMC).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
T-cell-specific surface glycoprotein CD28 (UniProt: P10747; also known as TP44, CD28) is encoded by the CD28 gene (Gene ID: 940) in human. CD28 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 19-152), a transmembrane domain (aa 152-179), and a cytoplasmic domain (aa 180-220). It is mainly expressed on T lineage cells, including thymocytes, but may also be found on plasmablasts. It is reported to be constitutively expressed on both resting and activated T cells, on almost all CD4+ and on at least half of CD8+ T cells in humans. It is involved in T cell activation, proliferation, survival, and cytokine production. It reduces the threshold of TCR triggering required for T cell activation. CD28 signaling transduces a positive signal that promotes proliferation of TCR-stimulated T cells. It is shown to cause the initial activation of nave CD4 T cells by increasing the sensitivity of T cells to antigen receptor engagement. It also enhances the production of IL-4 and IL-10 in T-cells in conjunction with TCR/CD3 ligation and CD40L co-stimulation. Upon activation of mature T cells, either by mitogens or by cross-linking of the TCR, the expression of CD28 is shown to increase. Ligation of CD3 and CD28 (CD3/28 co-stimulation) is shown to promote increase in glucose metabolism, increased cytokine/chemokine expression, elevate IL-2 production, and induce resistance to apoptosis. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Riley, JL., and June, CH. (2005). Blood. 105(1); 13-21).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Reconstitution
1.2 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, GermanyDisclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Параметры
| Quality Level | 200 |
| biological source | rabbit |
| recombinant | expressed in HEK 293 cells |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 2N15, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® |
| form | lyophilized |
| mol wt | calculated mol wt 25.07 kDa","observed mol wt ~40 kDa |
| purified by | using Protein A |
| species reactivity | human |
| species reactivity (predicted by homology) | bovine, monkey, rat |
| packaging | antibody small pack of 25 µL |
| enhanced validation | recombinant expression |
| application(s) | flow cytometry: suitable","immunocytochemistry: suitable","immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable","western blot: suitable |
| isotype | IgG |
| epitope sequence | C-terminal half |
| conjugate | unconjugated |
| P
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