Anti-CD25 (IL2-RA) Antibody, clone 1K6 ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1412-4X25UL

ZRB1412-25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.,
Specificity
Clone 1K6 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects IL-2 receptor subunit alpha (CD25). It targets an epitope within 119 amino acids from the N-terminal region.
GST/His-tagged recombinant fragment corresponding to 119 amino acids from the N-terminal, extracellular domain of human IL-2 receptor subunit alpha (CD25).
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CD25 (IL2-RA) in human lymph node tissue lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CD25 (IL2-RA) in human tonsil tissue sections.
Flow Cytometry Analysis: 1µg from a representative lot detected CD25 (IL2-RA) in one million human peripheral blood mononuclear cells (PBMC).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Anti-CD25 (IL2-RA), clone 1K6 ZooMAb, Cat. No. ZRB1412, is a recombinant Rabbit monoclonal antibody that targets CD25 (IL2-RA) and is tested for use in Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting.
Target description
Interleukin-2 receptor subunit alpha (UniProt: P01589; also known as IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55, CD25) is encoded by the IL2RA gene (Gene ID: 3559) in human. IL-2-RA is a non-covalent dimer of an alpha (CD25), beta unit (CD122). IL-2-RA is involved in the regulation of immune tolerance and controls the activity of T cells. IL-2RA can exist in three different forms; a high affinity dimer, an intermediate affinity monomer of beta subunit, and a low affinity monomer of alpha subunit. The high and intermediate affinity forms can also associate with a gamma subunit (CD132). CD25 is rapidly up-regulated upon activation of T cells. Activated T cells, regulatory T cells (Tregs) and NK cells express high levels of CD25, and high affinity expression of IL-2-RA is largely limited to these cells. CD25 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 22-240), a transmembrane domain (aa 241-250), and a short cytoplasmic tail (aa 260-272). It also contains two Sushi domains (aa 22-84 and 123-186). Mutations in IL2RA gene have been linked to insulin-dependent diabetes mellitus and immunodeficiency with lymphoproliferation that leads to recurrent viral, fungal, and bacterial infections. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. Ref.: (Driesen, J., et al. (2008). Immunobiology 213(9-10); 849-858).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
0.3 mg/mL after reconstitution at 25μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommended storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
mol wt	calculated mol wt 30.82 kDa, observed mol wt ~35 kDa
species reactivity	mouse, human
packaging	pkg of 25 µL
greener alternative product characteristics	Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry,.
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	flow cytometry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
isotype	IgG
conjugate	unconjugated
greener alternative category	Aligned,
shipped in	ambient
storage temp.	2-8°C

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