Anti-BSN Antibody, clone 2I2 ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1779-4X25UL

ZRB1779-25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 2I2 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Protein Bassoon (BSN). It targets an epitope within the C-terminal half.
GST/His-tagged recombinant fragment corresponding to 150 amino acids from the C-terminal half of human Protein Bassoon (BSN).
Anti-BSN, clone 2I2 ZooMAb®, Cat. No. ZRB1779, is a rabbit recombinant monoclonal antibody that specifically targets Protein Bassoon (BSN) and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry, and Western Blotting.
Quality Control Testing
Evaluated by Western Blotting in U251 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected BSN in U251 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected BSN in PC12 cell lysate.
Affinity Binding Assay: A representative lot of this antibody bound BSN peptide with a KD of 1.6 x 10-7 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected BSN in human cerebral cortex and human retina tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected BSN in E18 Rat hippocampal neurons.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Protein bassoon (UniProt: Q9UPA5; also known as Zinc finger protein 231, BSN) is encoded by the BSN (also known as KIAA0434, ZNF231) gene (Gene ID: 8927) in human. BSN is a multi-domain peripheral membrane protein that is exclusively expressed in brain. It serves as a scaffold protein of the presynaptic cytomatrix at the active zone, the region in the synapse where neurotransmitter is released. At the presynaptic active zone, it regulates the spatial organization of synaptic vesicle cluster, the protein complexes that execute membrane fusion and compensatory endocytosis. It promotes vesicular replenishment and, consequently, a large standing pool of readily releasable synaptic vesicles at the endbulb synapse. BSN are transported on Golgi-derived membranous organelles, called Piccolo-Bassoon transport vesicles (PTVs), from the neuronal soma to distal axonal locations, where they participate in assembling new synapses. It is known to mediate synapse to nucleus communication leading to reconfiguration of gene expression by associating with the transcriptional corepressor CTBP1 and by subsequently reducing the size of its pool available for nuclear import. Mutant mice deficient in functional Bassoon are reported to suffer from epileptic seizures and have dysfunctional hippocampal synapses. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Dresbach, T., et al. (2003). Mol. Cell. Neurosci. 23(2); 279-291; Fejtova, A., et al. (2009). J. Cell Biol. 185(2); 341-355; Schultz AM., et al. (2014). EMBO J. 33(4); 512-527).
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200,
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	2I2, monoclonal, recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
mol wt	calculated mol wt ~ 416.47 kDa, observed mol wt ~ 240 kDa
species reactivity	rat, human
packaging	antibody small pack of 25 µL
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	affinity binding assay: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
isotype	IgG
conjugate	unconjugated
shipped in	ambient
storage temp.	2-8°C

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable