Anti-BRSK2 (N-terminal) antibody produced in rabbit SAB4200431-200UL

NACRES:

NA.44

Brain selective kinase 2 (BRSK2) (SAD-A) protein is a member of the family Ser/Thr 5′ adenosine monophosphate-activated protein kinase (AMPK) related kinases. This protein is specifically located in the spinal cord and brain of embryonic and postnatal animals. The BRSK2 gene is located on the human chromosome at 11p15.5.
Specificity
Anti-BRSK2 (N-terminal) specifically recognizes mouse BRSK2.
synthetic peptide corresponding to a sequence near the N-terminus of human BRSK2, conjugated to KLH. The corresponding sequence is identical in rat and mouse BRSK2.
Anti-BRSK2 (N-terminal) antibody produced in rabbit may be used in immunoblotting and immunofluorescence.
Biochem/physiol Actions
Brain selective kinase 2 (BRSK2) protein is phosphorylated by liver kinase B1 (LKB1) at Thr-174 thereby increasing its kinase activity. This protein is also phosphorylated at Thr-260 by cyclic adenosine monophosphate (cAMP)-dependent protein kinase A (PKA) which is another upstream kinase. Brain selective kinase1/2 (BRSK1/2) phosphorylates downstream effectors like the cell cycle checkpoint kinase Wee1 and microtubule-associated protein tau. Phosphorylation of Wee1 by BRSK1/2 is essential for the regulation of its activity in polarized neurons and is an important step for the differentiation of polarized neurons.
Physical form
Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is also not recommended. If slight turbidity occurs uponprolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
form	buffered aqueous solution
mol wt	antigen ~75 kDa
species reactivity	human, rat, mouse
enhanced validation	recombinant expression
concentration	~1.0 mg/mL
application(s)	dot blot: 1-2 µg/mL using HEK-293T cell lysates overexpressing mouse BRSK2.","indirect immunofluorescence: 0.1-0.2 µg/mL using HEK-293T cells overexpressing mouse BRSK2.
conjugate	unconjugated
UniProt accession no.	Q8IWQ3,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... BRSK2(9024) mouse ... Brsk2(75770) rat ... Brsk2(293631)

RIDADR	NONH for all modes of transport
Flash Point F	Not applicable
Flash Point C	Not applicable