Anti-BRSK1 antibody produced in rabbit SAB4200392-200UL

NACRES:

NA.44

BRSK1 (BR serine/threonine kinase 1), also known as serine/threonine-protein kinase (SAD-1, SAD-B) and BRSK2 (SAD-A) belong to the family of Ser/Thr AMP-activated protein kinase (AMPK)-related kinases that are specifically expressed in the mammalian brain. BRSK1 gene is mapped to human chromosome 19q13.42. BRSK1 protein localizes in the synaptic vesicle in the hippocampus and cerebellum.
Specificity
Anti-BRSK1 specifically recognizes mouse BRSK1.
synthetic peptide corresponding to an internal sequence of human BRSK1, conjugated to KLH. The corresponding sequence is identical in mouse BRSK1.
Anti-BRSK1 antibody is suitable for use in western blot (2-4 μg/mL using S1 fraction extracts of mouse forebrain). The product can also be used for immunoprecipitation (10-20 μg) and indirect immunofluorescence (0.2-0.4 μg/mL) using HEK-293T cells overexpressing mouse BRSK1.
Biochem/physiol Actions
BRSK1 (BR serine/threonine kinase 1) is essential for the polarization of cortical neurons. Knock-out mice that lack both BRSK1 and BRSK2 have defects in neuronal polarity and die prematurely after birth. Liver kinase B1(LKB1) phosphorylates and activates the serine/threonine-protein (SAD) kinases at a specific threonine residue within the T-loop activation segment of the kinase domain. BRSK1/2 in turn phosphorylates downstream effectors such as the microtubule-associated protein, tau and the cell cycle checkpoint kinase, Wee1-like protein kinase (Wee1). Phosphorylation of Wee1 by BRSK1/2 is required to regulate its activity and differentiation of polarized neurons. Also, BRSK1/SADB is localized to centrosomes and to control centrosome duplication. SADB phosphorylates γ-tubulin on Ser131 residue, suggesting that it controls centrosome homeostasis by regulating the phosphorylation of γ -tubulin.
BRSK1 is a serine/threonine kinase that facilitates the G(2)/M cell cycle arrest upon UV- or methyl methane sulfonate-induced DNA damage. This kinase also modulates the polarity of neurons and duplication of centrosomes. Anti-BRSK1 antibody is specific for mouse BRSK1. The antibody is also expected to react with human BRSK1. In immunoblotting, detection of the BRSK1 band is specifically inhibited by the BRSK1 immunizing peptide.
Physical form
Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
form	buffered aqueous solution
mol wt	antigen ~90 kDa
species reactivity	mouse
enhanced validation	recombinant expression
concentration	~1.5 mg/mL
application(s)	immunoprecipitation (IP): 10-20 µg using lysates of HEK-293T cells overexpressing mouse BRSK1","indirect immunofluorescence: 0.2-0.4 µg/mL using HEK-293T cells overexpressing mouse BRSK1","western blot: 2-4 µg/mL using extracts of mouse forebrain (S1 fraction)
conjugate	unconjugated
UniProt accession no.	Q5RJI5,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... BRSK1(84446) mouse ... Brsk1(381979)

RIDADR	NONH for all modes of transport
Flash Point F	Not applicable
Flash Point C	Not applicable