Anti-BCRP (ABCG2) Antibody, clone 10K5 , ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1217-4X25UL

ZRB1217-25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.,
Specificity
Clone 10KS is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Breast cancer resistance protein (BCRP). It targets an epitope within the N-terminal cytoplasmic domain.
His-tagged recombinant fragment corresponding to 126 amino acids from the N-terminal, cytoplasmic domain of human Breast cancer resistance protein (BCRP).
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected BCRP (ABCG2) in human placenta and mouse placenta tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected BCRP (ABCG2) in HepG2, A549 and JEG-3 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens and experimental conditions may vary.
Anti-BCRP (ABCG2), clone 10K5 ZooMAb, Cat. No. ZRB1217, is a highly specific rabbit recombinant monoclonal antibody that targets BCRP (ABCG2) and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Target description
ATP-binding cassette sub-family G member 2 (UniProt: Q9UNQ0; also known as Breast cancer resistance protein, BCRP, CDw338, Mitoxantrone resistance-associated protein, Placenta-specific ATP-binding cassette transporter, Urate exporter, CD338) is encoded by the ABCG2 (also known as ABCP, BCRP, BCRP1, MXR) gene (Gene ID: 9429) in human. BCRP is a multi-pass membrane protein of the ABC transporter superfamily. It consists of six transmembrane domains and can functions as a homodimer or homotetramer. It contains an ABC transporter domain (aa 37-286) and an ABC transmembrane type-2 domain (aa 389-652). Unlike most of the other ABC transporters, BCRP contains only one nucleotide binding domain (aa 80-87). BCRP is highly expressed in placenta and lower expression is reported in small intestine, liver, and colon. It is also expressed in stem cells of different tissues and may be used as stem cell marker. It serves as a high-capacity urate exporter that functions in both renal and extrarenal urate excretion. It also serves as a xenobiotic transporter that plays an important role in the exclusion of xenobiotics from the brain. BCRP is overexpressed in many hematological malignancies and in solid tumors and contributes to resistance to a variety of anticancer agents. It has been implicated in the efflux of numerous drugs, including camptothecin, methotrexate, azidothymidine (AZT), daunorubicin, and doxorubicin. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit (recombinant)
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	10k5, recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
mol wt	calculated mol wt 72.31 kDa, observed mol wt ~72 kDa
species reactivity	human, mouse
packaging	antibody small pack of 25 µL
greener alternative product characteristics	Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry,.
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
isotype	IgG
conjugate	unconjugated
greener alternative category	Aligned,
shipped in	ambient
storage temp.	2-8°C

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable