Anti-Bak Antibody, clone 2D18 ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1453-4X25UL

ZRB1453-25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.,
Specificity
Clone 2D18 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Bcl-2 homologous antagonist/killer (BAK). It targets an epitope within 16 amino acids from the N-terminal region.
KLH conjugated linear peptide corresponding to 16 amino acids from the N-terminal region of human Bcl-2 homologous antagonist/killer (BAK).
Anti-Bak, clone 2D18 ZooMAb, Cat. No. ZRB1453, is a recombinant Rabbit monoclonal antibody that specifically detects Bak and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Bak in RAW264.7 cell lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Bak in human urinary bladder tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Bak in Jurkat cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Bcl-2 homologous antagonist/killer (UniProt: Q16611; also known as Apoptosis regulator BAK, Bcl-2-like protein 7, Bcl2-L-7, BAK) is encoded by the BAK1 (also known as BAK, BCL2L7, CDN1) gene (Gene ID: 578) in human. BAK is a single-pass membrane protein that plays a role in the mitochondrial apoptosis process. It is homodimeric protein whose homodimerization is zinc-dependent. It can also form heterodimers with BCL2 and BCL2L1. It is expressed in a wide variety of tissues, with highest levels displayed in heart and skeletal muscle. In non-apoptotic cells, BAK constitutively resides in the mitochondrial outer membrane, but upon arrival of cell death signals, it promotes mitochondrial outer membrane (MOM) permeabilization by oligomerizing to form pores within the MOM and releases apoptogenic factors into the cytosol, including cytochrome c, promoting the activation of caspase 9. BAK contains three BH motifs (BH1: aa 117-136; BH2: aa 169-184; and BH3: aa 74-88) and intact BH3 motif is required by BIK, BID, BAD, and BAX for their pro-apoptotic activity and their interaction with anti-apoptotic members of the Bcl-2 family. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit (recombinant)
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	2D18, monoclonal, recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
mol wt	calculated mol wt 23.41 kDa, observed mol wt 23 kDa
species reactivity	mouse, human, rat
packaging	antibody small pack of 25 µL
greener alternative product characteristics	Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry,.
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
isotype	IgG
conjugate	unconjugated
greener alternative category	Aligned,
shipped in	ambient
storage temp.	2-8°C

WGK Germany

WGK 1