Anti-Arc (N-terminal) antibody produced in rabbit SAB4200461-200UL

NACRES:

NA.41

Activity-regulated cytoskeleton-associated protein (Arc) is a growth factor and immediate-early gene (IEG) that is enriched in the brain. Arc is located in the distal dendrites and spine. The Arc gene is located on the human chromosome at 8q24.3.
Specificity
Anti-Arc(N-terminal) specifically recognizes humanand rat Arc.
synthetic peptide corresponding to a sequence at the N-terminal region of human Arc, conjugated to KLH. The corresponding sequence is highly conserved (90% sequence identity) in rat and mouse Arc.
Anti-Arc (N-terminal) antibody produced in rabbit may be used in:

• immunoblotting
• immunoprecipitation
• immunohistochemistry

Biochem/physiol Actions
Activity-regulated cytoskeleton-associated protein (Arc) induction is required for late long-term potentiation (LTP) and memory consolidation. It also plays a role in LTP-induced cofilin phosphorylation and F-actin stabilization. This protein affects synaptic plasticity. Arc regulates the endocytosis of α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptors through its interaction with endocytic proteins endophilin2/3 and dynamin. It is associated with notch signaling and spine morphology.
Physical form
Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution bycentrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
form	buffered aqueous solution
mol wt	antigen ~55 kDa
species reactivity	human, rat
enhanced validation	recombinant expression
concentration	~1.0 mg/mL
application(s)	immunohistochemistry: 20-30 µg/mL using formalin-fixed, paraffin-embedded rat brain.","immunoprecipitation (IP): 2-4 µg using lysates of HEK-293T cells over-expressing human Arc.","western blot: 1.5-3.0 µg/mL using extracts of HEK-293T cells over-expressing human Arc.
conjugate	unconjugated
UniProt accession no.	Q7LC44,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... ARC(23237) rat ... Arc(54323)

RIDADR	NONH for all modes of transport
Flash Point F	Not applicable
Flash Point C	Not applicable