Anti-Anosmin Antibody, clone 2C17 ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1303-25UL

ZRB1303-4X25UL

ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 2C17 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Anosmin. It targets an epitope within 18 amino acids from the C-terminal region.
KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal region of human Anosmin.
Quality Control Testing
Evaluated by Western Blotting in mouse heart tissue lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Anosmin in mouse heart tissue lysate.
Tested applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected human recombinant Anosmin.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Anosmin in Jurkat cells.
ELISA Analysis: 300 µg/mL from a representative lot detected recombinant human Anosmin..
Affinity Binding Assay: A representative lot of this antibody bound Anosmin with a KD of 9.3 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Anosmin, clone 2C17 ZooMAb®, Cat. No. ZRB1303, is a recombinant Rabbit monoclonal antibody that detects Anosmin and is tested for use in Affinity Binding Assay, ELISA, and Immunocytochemistry, and Western Blotting.
Target description
Anosmin-1 (UniProt: Adhesion molecule-like X-linked, Kallmann syndrome protein) is encoded by the ANOS1 (also known as ADMLX, KAL, KAL1, KALIG1) gene (Gene ID: 3730) in human. Anosmin-1 is an extracellular matrix glycoprotein that is synthesized with a signal peptide (aa 1-24), which is subsequently cleaved off. It is mainly a peripheral membrane protein, but it can undergo proteolytic cleavage and released into the extracellular space. It contains an N-terminal cysteine-rich region, a whey acidic protein-like four disulfide core motif (WAP), and four tandem fibronectin type III (FnIII)-like repeats, followed by a histidine-rich C terminus. Anosmin-1 is expressed in olfactory bulb, cerebellum, spinal cord, kidney, and retina during development. It is shown to be essential for axonal guidance and migration of olfactory and gonadotropin-releasing hormone (GnRH) neurons from the nasal placode to their final location in the brain. It also serves as a chemoattractant for fetal olfactory epithelial cells. It interacts with FGFR1 and induces neurite outgrowth and cytoskeletal rearrangement in embryonic GnRH olfactory neuroblasts. Mutations in ANOS1 gene resulting in loss of function are known to cause Kallmann syndrome that is characterized by anosmia and hypogonadism due to olfactory bulb dysfunction. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Gonzalez-Martinez, D., et al. (2004). J. Neurosci. 24(46); 10384-10392).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200
biological source	rabbit
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	2C17, recombinant monoclonal
description	recombinant, expressed in HEK 293 cells
product line	ZooMAb®
form	lyophilized
mol wt	calculated mol wt 76.11 kDa","observed mol wt ~80 kDa
purified by	using Protein A
species reactivity	human, mouse
species reactivity (predicted by homology)	monkey
packaging	antibody small pack of 25 µL
enhanced validation	recombinant expression
application(s)	ELISA: suitable","affinity binding assay: suitable","immunocytochemistry: suitable","western blot: suitable
isotype	IgG
epitope sequence	C-terminal
conjugate	unconjugated
Protein ID accession no.	NP_000207,
UniProt accession no.	P23352,
shipped in	ambient
storage temp.	2-8°C