Anti-Angiopoietin-2 Antibody, clone 4L18 ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1414-4X25UL

ZRB1414-25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.,
Specificity
Clone 4L18 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Angiopoietin-2. It targets an epitope within 23 amino acids from the N-terminal region.
KLH-conjugate linear peptide corresponding to 23 amino acids from the N-terminal region of human Angiopoietin-2.
Anti-Angiopoietin-2, clone 4L18 ZooMAb, Cat. No. ZRB1414, is a recombinant Rabbit monoclonal antibody that targets Angiopoietin-2 and is tested in Affinity Binding, Immunohistochemistry, Immunofluorescence, Western Blotting.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Angiopoietin-2 in post-fixed, frozen human placenta tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Angiopoietin-2 in human placenta tissue sections.
Affinity Binding Assay:
Affinity Binding Assay: A representative lot of this antibody bound Angiopoietin-2 peptide with a KD of 4.6 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Angiopoietin-2 (UniProt: O15123; also known as ANG-2) is encoded by the ANGPT2 gene (Gene ID: 285) in human. Angiopoietins are a family of growth factors that include Angiopoietin 1 and 2 and the orthologs 3 (in mouse) and 4 (in human). ANG-2 is a secreted glycoprotein that is expressed predominantly by endothelial cells and some smooth muscle cells in a tightly regulated manner. High expression of ANG-2 has been observed in placenta. In quiescent mature vessels its expression is shown to be low; however, upon receipt of inflammatory signals its levels are significantly elevated. ANG-2 binds to TEK/TIE2 and competes for the Angiopoietin-1 (ANG-1) binding site and modulates ANG-1 signaling. It is also reported to induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANG-1. Both ANG-1 and ANG-2 have been implicated in tumor angiogenesis and ANG-2 is highly up-regulated in the vasculature of mouse and human neoplasms. It also acts as a chemoattractant and mediates the migration of a monocytes that express Tie2 receptor. ANG-2 contains a fibrinogen C-terminal domain (aa 275-495) that mediates its interaction with the TEK/TIE2 receptor. Three isoforms of ANG-2 have been described that are produced by alternative splicing. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Scholz, A., et al. (2015). Ann. N.Y. Acad. Sci. 1347; 45-51; Felcht, M., et al. (2012). J. Clin. Invest. 122(6); 1991-2005).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
0.3 mg/mL after reconstitution at 25μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommended storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
mol wt	calculated mol wt 56.92 kDa, observed mol wt ~48 kDa
species reactivity	rat, human
packaging	pkg of 25 µL
greener alternative product characteristics	Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry,.
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	affinity binding assay: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, western blot: suitable
isotype	IgG
conjugate	unconjugated
greener alternative category	Aligned,
shipped in	ambient
storage temp.	2-8°C

WGK Germany

WGK 1