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Anti-ALK-7 Antibody, clone 1N5 ZooMAb® Rabbit Monoclonal
Гарантия 1 год
- Производитель:
- Sigma-Aldrich
- Производитель:
- Sigma-Aldrich
| Кат. номер |
| ZRB1384-4X25UL |
| ZRB1384-25UL |
General description
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 1N5 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects human Activin receptor-like kinase 7 (ALK-7). It targets an epitope within 17 amino acids from the cytoplasmic domain within the N-terminal half.Immunogen
KLH-conjugated linear peptide corresponding to 17 amino acids from the cytoplasmic domain within the N-terminal half of human Activin receptor-like kinase 7 (ALK-7), isoform 1.Application
Quality Control TestingEvaluated by Western Blotting in Human prostate tissue lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected ALK-7 in Human prostate tissue lysate.
Tested applications
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ALK-7 in PC3 cells.
Flow Cytometry Analysis: 1 µg from a representative lot detected ALK-7 in one million PC3 cells.
Affinity Binding Assay: A representative lot of this antibody bound ALK-7 Peptide with a KD of 1.0 x 10-12 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-ALK-7, clone 1N5 ZooMAb®, Cat. No. ZRB1384, is a recombinant Rabbit monoclonal antibody that specifically targets ALK-7 and is tested for use in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, and Western Blotting.
Target description
Activin receptor type-1C (UniProt: Q8NER5, also known as EC:2.7.11.30, Activin receptor type IC, ACTR-IC, Activin receptor-like kinase 7, ALK-7) is encoded by the ACVR1C (also known as ALK7) gene (Gene ID: 130399) in human. ALK-7 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-20) that is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 21-113), a transmembrane domain (aa 114-134, and a cytoplasmic domain (aa 135-493). ALK-7 expression is reported in pancreas, heart, colon, small intestine, ovary and the hippocampus, medulla oblongata and putamen of the brain. It serves as a transmembrane type IC receptor serine kinase for activin or activin-like ligands. The receptor complex consists of 2 type II and 2 type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which auto-phosphorylate, then bind and activate SMAD transcriptional regulators, SMAD2 and SMAD3. The type I receptors contains a Ser/Thr kinase domain in its cytoplasmic domain (aa 194-485) that is essential for signaling, while the type II are receptors are essential for ligand binding. ALK-7 is reported to negatively regulate glucose-stimulated insulin release in -cells. Mice lacking ALK-7 display normal pancreas organogenesis but develop an age-dependent syndrome involving progressive hyperinsulinemia, reduced insulin sensitivity, liver steatosis, impaired glucose tolerance, and islet enlargement. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Roberts, HJ., et al. (2003). Biol. Reprod. 68(5); 1719-1726).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, GermanyDisclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Параметры
| Quality Level | 200 |
| biological source | rabbit |
| recombinant | expressed in HEK 293 cells |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1N5, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® |
| form | lyophilized |
| mol wt | calculated mol wt 54.87 kDa","observed mol wt ~61 kDa |
| species reactivity | human |
| species reactivity (predicted by homology) | monkey |
| packaging | antibody small pack of 25 µL |
| enhanced validation | recombinant expression |
| application(s) | affinity binding assay: suitable","flow cytometry: suitable","immunocytochemistry: suitable","western blot: suitable |
| isotype | IgG |
| epitope sequence | N-terminal half |
| conjugate | unconjugated |
| Protein
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