- Производитель:
- Sigma-Aldrich
Кат. номер |
ZRB1099-25UL |
ZRB1099-4X25UL |
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.,
Specificity
Clone 4J6 ZooMAb is a rabbit recombinant monoclonal antibody that specifically detects human Retinal Dehydrogenase 1. It targets an epitope within 19 amino acids from the C-terminal region.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected ALDH1A1 in human stomach and human liver tissue sections.
Immunoprecipitation Analysis: 3 µg from a representative lot immunoprecipitated ALDH1A1 in HepG2 cell lysate.
Immunofluorescence Analysis: A 1:10-1:100 dilution from a representative lot detected ALDH1A1 in human stomach and human liver tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Anti-ALDH1A1, clone 4J6 ZooMAb , Cat. No. ZRB1099, is a highly specific rabbit recombinant monoclonal antibody that targets ALDH1A1 and has been tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
Target description
Retinal dehydrogenase 1 (UniProt: P00352; also known as RALDH 1, RalDH1, ALDH-E1, ALHDII, Aldehyde dehydrogenase family 1 member A1, Aldehyde dehydrogenase, cytosolic) is encoded by the ALDH1A1 (also known as ALDC, ALDH1, PUMB1) gene (Gene ID: 216) in human. Retinal dehydrogenase 1 is a ubiquitously distributed cytosolic enzyme that serves as a detoxification enzyme and generates retinoic acid. It catalyzes the oxidation of retinal to retinoic acid that is essential for differentiation, development, and survival of dopaminergic neurons. It can also play a role in cellular defense against oxidative stress by oxidizing 4-hydroxynonenal (4-HNE) produced during lipid peroxidation. It is also involved in several other biological processes, such as stem cell maintenance, UV-radiation resistance, melanogenesis, and spermatogenesis, by catalyzing the conversion of specific aldehydes to their corresponding carboxylic acids. Retinal dehydrogenase 1 contains multiple NAD binding sites. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Paval, D., et al. (2017). Clin. Psychopharmacol. Neurosci. 5(3); 229-236).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source | rabbit (recombinant) |
recombinant | expressed in HEK 293 cells |
antibody form | purified antibody |
antibody product type | primary antibodies |
clone | 4J6, recombinant monoclonal |
product line | ZooMAb® learn more, |
form | lyophilized |
mol wt | calculated mol wt 54.86 kDa, observed mol wt ~54 kDa |
species reactivity | human |
packaging | antibody small pack of 25 µL |
greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry,. |
enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation, |
application(s) | immunofluorescence: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, western blot: suitable (peptide) |
isotype | IgG |
conjugate | unconjugated |
shipped in | ambient |
storage temp. | 2-8°C |
WGK Germany | WGK 1 |
Flash Point F | Not applicable |
Flash Point C | Not applicable |
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