Anti-Ago2 Antibody, clone 2H10 ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1205-4X25UL

ZRB1205-25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 2H10 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Protein Argonaute -2 (Ago2). It targets an epitope within 23 amino acids from the N-terminal region.
KLH-conjugated linear peptide corresponding to 23 amino acids from the N-terminal region of human Protein argonaute-2 (Ago2).
Anti-Ago2, clone 2H10 ZooMAb®, Cat. No. ZRB1205, is a rabbit recombinant monoclonal that specifically targets Ago2 and is tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Quality Control Testing
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Ago2 in HeLa cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Ago2 in MCF-7, NIH3T3, and C6 cell lysates.
Flow Cytometry Analysis: 1 µg from a representative lot detected Ago2 in HeLa cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Ago2 in human cerebral cortex tissue sections.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Ago2 in NIH3T3 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Protein argonaute-2 (UniProt: Q9UKV8; also known as Argonaute2, hAgo2, Argonaute RISC catalytic component 2, Eukaryotic translation initiation factor 2C, eIF-2C, eIF2C, PAZ Piwi domain protein, PPD, Protein slicer) is encoded by the AGO2 (also known as EIF2C2) gene (Gene ID: 27161) in human. Ago2 is a highly conserved member of the AGO family of proteins with catalytic activity and is required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by Ago2 and binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation and is independent of endonuclease activity. Ago2 may inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. Ago2 is shown to have four core domains that are known as N domain, PAZ domain (aa 235-348), MID domain, and PIWI domain (aa 517-818). The PAZ domain is an RNA binding module that recognizes the 3 end of both siRNA and miRNA, in a sequence independent manner and is reported to be strictly required for Ago2 to form the RISC complex. The PIWI domain has major roles within Ago2, including RNase H like endonucleolytic activity and regulating the interaction between MID domain and DNA/RNA. Ago2 is shown to undergo 4-hydoxylation that enhances its stability but is not essential for miRNA binding or endonuclease activity. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Ye, Z., et al. (2015). J. Cancer 6(9); 877-882).
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200,
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	2H10, monoclonal, recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
mol wt	calculated mol wt ~ 97.21 kDa, observed mol wt ~ 97 kDa
species reactivity	mouse, human, rat
packaging	antibody small pack of 25 µL
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
isotype	IgG
conjugate	unconjugated
shipped in	ambient
storage temp.	2-8°C

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable