Anti--Actin Antibody, clone 6J13 ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1166-25UL

ZRB1166-4X25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 6J13 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects g-Actin. It targets an epitope within 13 amino acids from the N-terminal.
KLH-conjugated linear peptide corresponding to 13 amino acids from the N-terminal region of human g-Actin.
Anti-g-Actin, clone 6J13 ZooMAb®, Cat. No. ZRB1166, is a recombinant Rabbit monoclonal antibody that specifically targets g-Actin and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Quality Control Testing
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected y-Actin in HepG2 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected y-Actin in HeLa, C6, and mouse kidney tissue lysates.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected g-Actin in human small intestine tissue sections.
Flow Cytometry Analysis: 0.1 µg from a representative lot detected g-Actin in one million HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected g-Actin in HepG2 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Actin, cytoplasmic 2 (UniProt: P63261; also known as Gamma-actin) is encoded by the ACTG1 (also known as ACTG) gene (Gene ID: 71) in human. Actins are ubiquitous eukaryotic proteins that serve as multi-functional, basic building blocks of cytoskeletal microfilaments. They play critical roles in a wide range of cellular processes, including cell division, cell migration, chromatin remodeling, transcriptional regulation, and vesicle trafficking. These functions are attributed to their ability to form filaments, which can quickly assemble and disassemble depending upon the needs of the cell. At least six different actin types have been reported in mammals. Although actins show about 90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expression patterns and only 50-60% homology is found in their 18 N-terminal residues. -actin is a cytoplasmic actin that is highly conserved in higher animals and is predominantly expressed in non-muscle cells where it controls cell structure and motility. Unlike -actin that is enriched at the leading edge of migrating cells, -actin is more uniformly distributed throughout the cell. -actin knockout is reported to cause developmental delays, reduced postnatal and adult survival, and progressive myopathy. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Belyantseva, EA., et al. (2009). Proc. Natl. Acad. Sci. USA. 106(24); 9703-9708).
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200,
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	6J13, monoclonal, recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
mol wt	calculated mol wt ~ 41.79 kDa, observed mol wt ~ 42 kDa
species reactivity	rat, mouse, human
species reactivity (predicted by homology)	bovine
packaging	antibody small pack of 25 µL
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
isotype	IgG
conjugate	unconjugated
shipped in	ambient
storage temp.	2-8°C

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable