Anti-ACAD10 Antibody, clone 3J20 ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1828-25UL

ZRB1828-4X25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 3J20 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Acyl-CoA dehydrogenase family member 10 (ACAD10). It targets an epitope within 18 amino acids from the C-terminal half.
KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal half of human Acyl-CoA dehydrogenase family member 10 (ACAD10).
Anti-ACAD10, clone 3J20 ZooMAb®, Cat. No. ZRB1828, is a recombinant Rabbit monoclonal antibody that specifically targets ACAD10 and is tested for use in Affinity Binding Assay, Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting.
Quality Control Testing
Evaluated by Western Blotting in L6 cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected ACAD10 in L6 cell lysate.
Tested Applications
Affinity Binding Assay: A representative lot of this antibody bound ACAD10 peptide with a KD of 1.0 x 10-12 in an affinity binding assay.
Flow Cytometry Analysis: 0.1 µg from a representative lot detected ACAD10 in one million L6 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected ACAD10 in human skeletal muscle tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Acyl-CoA dehydrogenase family member 10 (UniProt: Q6JQN1; also known as ACAD-10) is encoded by the ACAD10 gene (Gene ID: 80724) in human. Acyl-CoA dehydrogenases (ACADs) are a family of flavoproteins that are involved in the beta-oxidation of the fatty acyl-CoA derivatives to generate acetyl CoA and reducing equivalents for generating energy. ACAD-10 is a widely expressed mitochondrial matrix enzyme. Its high expression has been reported in fetal brain. It is also highly expressed in liver, kidney, pancreas, and spleen. It contains a hydrolase domain at the N-terminal region, a serine/threonine protein kinase catalytic domain in the central region, and a conserved ACAD domain in the C-terminal region. It catalyzes the mitochondrial beta-oxidation of fatty acyl-CoA derivatives. Variations in ACAD10 gene have been linked to increased susceptibility to Type II diabetes, which may be mediated by impaired insulin sensitivity and abnormal lipid oxidation. Mice deficient in ACAD10 display abnormal glucose tolerance and elevated insulin levels. They accumulate excess abdominal fat, develop early liver inflammation, and have abnormal skeletal muscle mitochondria. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Bian, L., et al. (2010). Diabetologia. 53(7); 1349-1353; He, M., et al. (2011). Mol. Genet. Metab. 102(4); 418-429; Bloom, K., et al., (2017). J. Inhert. Metab. Dis. 41(1); 49-57).
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200,
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	3J20, monoclonal, recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
mol wt	calculated mol wt ~ 118.83 kDa, observed mol wt ~ 130 kDa
species reactivity	human, rat
species reactivity (predicted by homology)	monkey
packaging	antibody small pack of 25 µL
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	affinity binding assay: suitable, flow cytometry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
isotype	IgG
conjugate	unconjugated
shipped in	ambient
storage temp.	2-8°C

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable