Anti-14-3-3 / Antibody, clone 1D15 ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1856-4X25UL

ZRB1856-25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 1D15 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects 14-3-3 protein /a. It targets an epitope within 19 amino acids from the C-terminal half.
KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal half of human 14-3-3 protein β/a.
Anti-14-3-3 /a, clone 1D15 ZooMAb®, Cat. No. ZRB1856, is a recombinant Rabbit monoclonal antibody that detects 14-3-3 /a and is tested for use in Affinity Binding Assay, ELISA, and Western Blotting.
Quality Control Testing
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected 14-3-3 /a in HeLa cell lysate.
Tested Applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected 14-3-3 /a in SHSY5Y and COS-1 cell lysates.
ELISA Analysis: 1.0 µg/mL from a representative lot detected recombinant 14-3-3 /a.
Affinity Binding Assay: A representative lot of this antibody bound 14-3-3 b/a peptide with a KD of 2.3 x 10-6 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
14-3-3 protein /a (UniProt: P31946; also known as Protein 1054, Protein kinase C inhibitor protein 1, KCIP-1) is encoded by the YWHAB gene (Gene ID: 7529) in human. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and heterodimers, thus rendering 14-3-3 as a key multifunctional regulatory molecule. 14-3-3 protein beta/alpha serves as an adapter protein that has been implicated in the regulation of a large spectrum of both general and specialized signaling pathways. It binds to many partners, usually by recognition of a phosphoserine or phosphothreonine motif and its binding generally results in the modulation of the activity of the binding partner. The alpha, brain-specific form, differs from the beta form in its ability to be phosphorylated on serine 60 by protein kinase C delta in a sphingosine-dependent fashion. 14-3-3 /a is shown to block the nuclear translocation of the phosphorylated form of SR protein-specific kinase 2 (SRPK2) and antagonize its stimulatory effect on cyclin D1 expression that results in blockage of SRPK2 elicited neuronal apoptosis. It also reported to serve as a negative regulator of osteogenesis. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200,
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	1D15, monoclonal, recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
mol wt	calculated mol wt ~ 28.08 kDa, observed mol wt ~ 28 kDa
species reactivity	human, monkey
species reactivity (predicted by homology)	sheep, bovine, rat
packaging	antibody small pack of 25 µL
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	ELISA: suitable, affinity binding assay: suitable, western blot: suitable
isotype	IgG
conjugate	unconjugated
shipped in	ambient
storage temp.	2-8°C

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable