Human TNF-alpha ELISA Kit (for Lysates) RAB1089-1KT

NACRES:

NA.32

Tumor necrosis factor-α (TNF-α) is encoded by the gene mapped to human chromosome 6p21.3, within the major histocompatibility complex (MHC) class III region. The encoded protein belongs to the TNF superfamily.
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human ANG, CD23, Eotaxin, GCSF, GM-CSF, GRO-alpha, GRO-beta, GRO-gamma, I-309, IFN-gamma, IL-1 alpha, IL-1 beta, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40), IL-12 (p70), IL-15, IL-16, IP-10, MCP-1, MCP-2, MCP-3, MCP-4, MCSF, MIG, MIP-1 alpha, MIP-1 beta, NAP-2, PDGF, PF-4
Please refer to the attached Protocol,for details.
Human tumor necrosis factor-alpha (TNF-) ELISA Kit has been used in enzyme-linked immunosorbent assay (ELISA) to quantify TNF- in supernates of human acute lung infection model (ALIM), human umbilical vein endothelial cells (HUVEC) and human microvascular endothelial cells (HMEC-1).
Biochem/physiol Actions
Tumor necrosis factor-α (TNF-α) is a key immunomodulator and proinflammatory cytokine. It is implicated in the immune pathogenesis of various autoimmune, inflammatory diseases and hepatitis B virus (HBV) infection. Mutation in the gene increases the risk of susceptibility to diffuse large B-cell lymphoma (DLBCL) and marginal zone lymphoma. In addition, polymorphism in the gene leads to obsessive-compulsive disorder (OCD) and osteoarthritis (OA).
A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number.

species reactivity	human
packaging	kit of 96 wells (12 strips x 8 wells)
application(s)	ELISA: suitable
input	sample type cell lysate sample type tissue lysate
assay range	inter-assay cv: <12% intra-assay cv: <10% sensitivity: 30 pg/ml
detection method	colorimetric
shipped in	wet ice
storage temp.	20°C
Gene Information	human ... TNF(7124)

pictograms	GHS05
signalword	Warning
hcodes	H290
RIDADR	UN 3264 8 / PGIII
Flash Point F	Not applicable
Flash Point C	Not applicable