Human Insulin ELISA Kit RAB0327-1KT

NACRES:

NA.32

The Human Insulin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Insulin and Proinsulin in serum, plasma, cell culture supernatants and urine.
Recombinant Human Insulin
Human Insulin ELISA Kit has been used:

• to measure the blood levels of human insulin and proinsulin in the transgenic mice
• to measure serum insulin indaibetic rats
• to measure insulin in Multiple Sclerosis patients

For research use only. Not for use in diagnostic procedures.
Human Insulin ELISA Kit has been used to determine human insulin and pro-insulin concentration in serum and HAMECs (human adipose microvascular endothelial cells) which were isolated from human visceral fat of nondiabetic patients.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Insulin is responsible for two types of actions, excitatory and inhibitory. In its excitatory role, it increases the uptake of glucose and lipid synthesis, and in its inhibitory role it inhibits glycogenolysis, gluconeogenesis, lipolysis, proteolysis and ketogenesis. Insulin plays a vital role in regulation of blood glucose level within the normal range. Abnormal insulin secretion leads to various disorders such as diabetes, hyperglycemia or hypoglycemia. Type I diabetes is a result of autoimmune destruction of β cells of pancreas, which leads to depletion of insulin. Mutant INS-gene Induced Diabetes of Youth (MIDY) syndrome is an autosomal dominant disorder caused by missense mutations, which lead to aberrant proinsulin folding. Impaired glucose tolerance (IGT) or non-insulin-dependent diabetes mellitus (NIDDM) is caused by resistance to insulin-stimulated glucose uptake.
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

species reactivity	human
packaging	kit of 96 wells (12 strips x 8 wells)
application(s)	ELISA: suitable, capture ELISA: suitable
input	sample type serum sample type cell culture supernatant(s) sample type urine sample type plasma
assay range	inter-assay cv: <12% intra-assay cv: <10% sensitivity: 4 µIU/mL standard curve range: 4.69-300 µIU/mL
detection method	colorimetric
shipped in	wet ice
storage temp.	20°C
Gene Information	human ... INS(3630)

pictograms	GHS05
signalword	Warning
hcodes	H290
RIDADR	UN 3264 8 / PGIII