Human Fas Ligand ELISA Kit RAB0178-1KT

NACRES:

NA.32

The Human Fas Ligand ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Fas Ligand in serum, plasma, cell culture supernatants and urine.
Recombinant Human Fas Ligand
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure ,(sandwich, competitive & Indirect ELISA)
Biochem/physiol Actions
Binding of Fas Ligand (FasL) to Fas receptor triggers apoptosis in Fas-bearing cells. FasL has the ability to kill T cells and activate B cells which leads to down-regulation of the immune response. The mechanism of Fas induced apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD (Fas-associated protein with death domain) followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to eventual cell death. FasL is also involved in AGE (advanced glycation end-product)-mediated apoptosis in human retinal ARPE-19 cells, suggesting its role in diabetic retinopathy. Changes in the activity of FasL suppress normal apoptosis, leading to abnormal survival and growth of tumor cells. Mutations in the FasL gene causes autoimmune lymphoproliferative syndrome (ALPS).
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

species reactivity	human
packaging	kit of 96 wells (12 strips x 8 wells)
application(s)	ELISA: suitable, capture ELISA: suitable
input	sample type plasma sample type cell culture supernatant(s) sample type serum
assay range	inter-assay cv: <12% intra-assay cv: <10% sensitivity: 2 pg/mL standard curve range: 1.37-1000 pg/mL
detection method	colorimetric
shipped in	wet ice
storage temp.	20°C
Gene Information	human ... FASLG(356)

pictograms	GHS05
signalword	Warning
hcodes	H290
RIDADR	UN 3264 8 / PGIII