C-Peptide EIA Kit RAB0326-1KT

NACRES:

NA.32

The C-Peptide Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting C-Peptide peptide based on the principle of Competitive Enzyme Immunoassay. EIA-CPE-1 only detects C-peptide, not Insulin A or B chain.
C-Peptide , synthetic peptide
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure ,(sandwich, competitive & Indirect ELISA)
Biochem/physiol Actions
C-peptide inhibits endothelial dysfunction under high glucose conditions via inhibition of nuclear factor-B (NF-B) pathway. Experimental studies suggest that prolonged dosage of physiological concentration of C-peptide to type 1 diabetes patients minimizes diabetes-induced complications, such as glomerular hypertrophy and mesangial matrix expansion by interrupting transforming growth factor 1 (TGF-1) and tumor necrosis factor (TNF) signaling pathway. C-peptide reduces diabetes-induced glomerular hyperfiltration by contraction of the afferent arteriole, dilation of the efferent arteriole, and by inhibition of tubular reabsorption. C-Peptide also plays a crucial role in renal pathophysiology. C-peptide binds to a cell surface receptor that is likely to be G-protein coupled. This interaction stimulates various cellular functions such as increasing intracellular Ca2+ concentration, enhancing PI3-K (phosphoinositide 3-kinase) activity, increased endothelial nitric oxide synthase (eNOS) transcription, and activation of the Na+/K+ ATPase and mitogen-activated protein kinase (MAPK) signaling pathway.

species reactivity	human, rat, mouse
packaging	kit of 96 wells (12 strips x 8 wells)
application(s)	ELISA: suitable, enzyme immunoassay: suitable
input	sample type cell lysate sample type culture supernatant(s) sample type plasma sample type serum
assay range	inter-assay cv: <15% intra-assay cv: <10% sensitivity: 772 pg/mL standard curve range: 0.1-1000 ng/mL
detection method	colorimetric
shipped in	wet ice
storage temp.	20°C
Gene Information	human ... INS(3630)

pictograms	GHS05
signalword	Warning
hcodes	H290
RIDADR	UN 3264 8 / PGIII