- Производитель:
- Roche
Кат. номер |
11920685001 |
11774425001 |
The Cell Death Detection ELISAPLUS photometric enzyme immunoassay is used for the quantitative in vitro determination of cytoplasmic histone-associated DNA fragments (mono- and oligonucleosomes) after induced cell death.
The kit contains a stop solution which allows users to terminate the substrate reaction and run the assay under defined conditions, making it suitable for use in high-throughput applications.
- One-step ELISA
- High sensitivity: (5 x 102 cells/ml)
- Fast performance: (3 - 4 hours)
- Positive control included
- No prelabeling of cells necessary
- Nonradioactive assay system
- No species restriction
- Easy handling
- Low background
- Suppressed human anti-mouse factor
- Function tested
- 11774425001 (1 kit containing 10 components)
- 11920685001 (1 kit containing 10 components)
1 kit containing 10 components
Negative control: Depending on cell culture conditions, each exponentially growing permanent cell culture contains a certain amount of dead cells (typically approximately 3 - 8%). In the immunoassay, these inherent dead cells in the untreated sample (without a cell-death-inducing agent) will cause a certain absorbance value (negative control).
Positive control: A DNA-histone complex serves as a positive control.
Sample material: Cytoplasmic fractions (lysates) of cell lines, cells ex vivo, cell culture supernatants, and serum or plasma
Alongside the ready-to-use solutions supplied with this kit, you will need to prepare the following working solutions:
Note: Use only double-distilled water for reconstitution of lyophilizates.
Anti-Histone Biotin
Reconstitute the lyophilizate in 450 µl double-distilled water for 10 minutes and mix thoroughly.
Use: Component of the Immunoreagent.
Anti-DNA Peroxidase
Reconstitute the lyophilizate in 450 µl double-distilled water for 10 minutes and mix thoroughly.
Use: Component of the Immunoreagent.
Positive Control
Reconstitute the lyophilizate in 450 µl double-distilled water for 10 minutes and mix thoroughly.
Use: ELISA Step 1.
ABTS Tablets
Dependent on the number of samples tested, dissolve 1, 2, or 3 tablets from bottle 7 in 5, 10, or 15 ml Substrate Buffer.
Use: ELISA Step 5.
ABTS Stop Solution
If turbidity or a precipitate is visible, warm to 37 °C with shaking until the solution is clear.
Use: ELISA Step 6.
Storage conditions (working solution): Anti-Histone Biotin: 2 to 8 °C for 2 months
ABTS Tablets: 1 month stored protected from light. Allow to come to 15 to 25 °C before use.
ABTS Stop Solution: 2 to 8 °C until the expiration date printed on the label.
The exact detection limit of dying/dead cells in a particular sample strongly depends on the kinetics of cell death, the cytotoxic agent used, and the amount of affected cells in the total cell population. Using U937/camptothecin (CAM) as a cellular model system for cell death, the immunoassay allows the specific detection of mono- and oligonucleosomes in the cytoplasmic fraction of 125cell equivalents/well.
usage | sufficient for 10 x 96 multiwell tests (11920685001), sufficient for 96 multiwell tests (11774425001) |
Quality Level | 100 |
Торговая марка | Roche |
shipped in | wet ice |
storage temp. | 2-8°C |
RIDADR | NONH for all modes of transport |
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