Duolink® PLA Multicolor Probemaker Kit - Green DUO96020-1KT

Specificity
Green fluorescence detection reagents are often used with FITC filter.
Application Note
Primary antibodies are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC), or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
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Features and Benefits
Multicolor Duolink® proximity ligation assay (PLA®), is an application of the Duolink® technology, that allows for endogenous, simultaneous detection of up to 4 protein interactions, post-translational modifications, or protein expression levels, (including low abundant protein) in fixed cells and tissue samples. Follow the Duolink® PLA Probemaker Guide for Multicolor Detection, and the Duolink® PLA Multicolor Detection protocol, to use this product.

• Visualize and detect multiple proteins events in one experiment.
• No overexpression or genetic manipulation required
• High specificity (fewer false positives)
• Detection of low abundant proteins due to rolling circle amplification
• Relative quantification possible
• No special equipment needed
• Faster and more simple than FRET
• Increased accuracy compared to co-IP
• Publication-ready results

Components
Duolink® PLA Multicolor Probemaker Kit - Green contains all the necessary reagents to perform the conjugations of Green Oligo C and GreenOligo D directly to primary antibodies. The resulting PLA probes (Green C and Green D) must be used together and cannot be paired with any other Duolink® PLA Probe.
This product is comprised of the following:

• Green Oligo C DUO86020C
• Green Oligo D DUO86020D
• Conjugation Buffer DUO82033
• Stop Reagent DUO82034
• Storage Solution DUO82035

See datasheet for more information.
To perform a Multicolor Duolink® PLA experiment primary antibodies (PLA, IHC, ICC or IF validated), that recognize protein targets of interest are required. Duolink® PLA Multicolor Probemaker-generated probes (Red A/Red B, Green C/Green D, Orange F/Orange G, and/or FarRed H/FarRed I) are used in combination to perform the Multiplex experiment.The reagents in the Duolink® PLA Multicolor Reagent Pack, are required to perform the amplification and detection of the resulting PLA probes. Required additional reagents include Duolink® Wash Buffers and Mounting Medium.
Duolink is a registered trademark of Sigma-Aldrich Co. LLC
PLA is a registered trademark of Sigma-Aldrich Co. LLC

product line	Duolink®
application(s)	immunofluorescence: suitable, multiplexing: suitable, proximity ligation assay: suitable
fluorescence	ex 490 nm; em 520 nm (FITC), ex 490 nm; em 520 nm (green)
suitability	suitable for fluorescence
shipped in	dry ice
storage temp.	20°C