Duolink® In Situ Microplate Nuclear Stain, Anti-Fade DUO82064-1KT

NACRES:

NA.32

Duolink®proximity ligation assay(PLA®), allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.
Use the Multiwell Plates modifications, to the Duolink® In Situ Fluorescence Protocol, to run an experiment with this product. A set of short instructions, can also be used.
Visit our Duolink® PLA Resource Center, for information on how to run a Duolink® experiment, applications, troubleshooting, and more.
To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated), that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
Specificity
Duolink® In Situ Microplate Nuclear Stain and Anti-Fade are intended to be used after staining cells with Duolink® In Situ in microtiter plates. See the datasheet for more information.

Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
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Features and Benefits

• No overexpression or genetic manipulation required
• High specificity (fewer false positives)
• Single molecule sensitivity due to rolling circle amplification
• Relative quantification possible
• No special equipment needed
• Quicker and simpler than FRET
• Increased accuracy compared to co-IP
• Publication-ready results

Duolink is a registered trademark of Sigma-Aldrich Co. LLC
PLA is a registered trademark of Sigma-Aldrich Co. LLC

Quality Level	200,
product line	Duolink®
application(s)	proximity ligation assay: suitable
fluorescence	ex 360 nm; em 460 nm
suitability	suitable for fluorescence-detection automated sequencing, suitable for microtiter plates
shipped in	dry ice
storage temp.	20°C

pictograms	GHS07,GHS09
signalword	Warning
hcodes	H317 - H411
pcodes	P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313
RIDADR	NONH for all modes of transport