- Производитель:
- Roche
Enzyme Commission (EC) Number: | 3.2.1.31 ( BRENDA | IUBMB ) |
Кат. номер |
3707580001 |
3707601001 |
3707598001 |
Glucuronidation is one of the basic principles of metabolism. Many substances presented to the human body undergo metabolic processing that includes conjugation with glucuronic acid by UDP-glucuronosyltransferases (UGTs). This enzyme catalyzes the transfer of a glucuronyl group to many biological and pharmacologically active endogenous and exogenous molecules. Glucuronide is in general, more soluble, less toxic, and more easily excreted by the human body compared to the original molecule. To analyze these drug conjugates that are present in body fluids, such as urine and plasma, deconjugation of the glucuronide is necessary.
Substances used for doping are conjugated with glucuronides, which means that effective doping analysis relies very often on the enzymatic cleavage of conjugated drug molecules by -glucuronidase.
Enzyme Characteristics
The E. coli -glucuronidase enzyme is essentially free of sulfatase activity and is especially efficient in cleaving steroid and benzodiazepine conjugates.
-D-Glucuronoside glucuronosohydrolase
EC 3.2.1.31
- for the hydrolysis of steroid conjugates (glucuronides) in urine (pH 6.0–6.5)
- in doping analysis
- for the detection of benzodiazepine in small doses.
- during sample preparation to cleave off glucuronides prior to GC-MS, HPLC, immunoassays, or other analytical methods.
- Perform fast analysis due to the enzymes high specific activity.
- Quickly screen for steroids, benzodiazepines, cannabinoids, and opioids.
- Save time by developing your procedure without the need to clean up the reaction or buffer the urine.
The Fishman unit was previously used. This unit is the release of phenolphthalein from its glucuronide (PPG). It is not possible to measure the relative activities of different preparations for steroid -glucuronides by comparing their activities with respect to PPG. Many preparation do not catalyze the hydrolysis of PPG, 4NPG, or the various steroid -glucuronides in urine equally. The choice of 4NPG as standard substrate is based on the following considerations:
- The Michaelis concentrations for the two substrates are similar (KM = 2 x10-4 M for 4 NPG and KM = 6 x10-5 M for PPG), but the corresponding rates of hydrolysis differ:
- 4NPG is hydrolysed about 5 x as fast as PPG;
- For PPG, inhibition by excess substrate occurs; this is not observed using 4NPG.
(15 ml in one bottle)
For life science research only. Not for use in diagnostic procedures.
Quality Level | 100 |
form | solution |
specific activity | ~80 units/mg protein (Glucuronidase at 25 °C, or 140 U/mg at 37 °C, at pH 7 with 4-nitrophenyl--D-glucuronide as substrate; 1 ml -Glucuronidase contains at least 140 U at 37 °C.) |
mol wt | Mr ~220 kDa |
packaging | pkg of 1 mL (03707580001), pkg of 15 mL (03707601001), pkg of 5 mL (03707598001) |
Торговая марка | Roche |
parameter | 48 °C optimum reaction temp. |
optimum pH | 6.0-6.5 |
shipped in | wet ice |
storage temp. | 2-8°C |
RIDADR | NONH for all modes of transport |
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