TWEEN® 20
CAS Number: | 9005-64-5 |
MDL number: | MFCD00165986 |
NACRES: | NA.21 |
Кат. номер |
P1379-1GA |
P1379-EW |
P1379-VAR |
P1379-102L |
P1379-25L |
P1379-100ML-KC |
P1379-100ML |
P1379-500ML |
P1379-1L |
P1379-250ML |
P1379-25ML |
P1379-4L |
P1379-6X1L |
P1379-6X500ML |
P1379-1KT |
Tween®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.
TWEEN 20 is a nonionic detergent widely used in biochemical applications. It has been used as an emulsifying agent for the preparation of stable oil-in-water emulsions. TWEEN 20 has been used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins).
TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%.
TWEEN 20 has been used as a component of:
- washing buffer and blocking buffer in Western blotting
- blocking buffer in immunohistochemistry
- washing buffer in ELISA (enzyme linked immunosorbent assay)
- reaction mixture in PCR (polymerase chain reaction)
Non-ionic detergent
1, 4 L in poly bottle
25, 100, 250, 500, 6×500 mL in poly bottle
P1379-1KT:
Each kit contains 3 x 100ML samples, each sample from a uniquely manufactured lot.
TWEEN 20 is miscible in water (100mg/mL), yielding a clear, yellow solution. It is also miscible with alcohol, dioxane, and ethyl acetate. TWEEN 20 is insoluble in liquid paraffin and fixed oils.
TWEEN is a registered trademark of Croda International PLC
Quality Level | 300 |
description | non-ionic |
assay | 40.0% (GC) |
form | viscous liquid |
mol wt | ~1228 |
composition | lauric acid, 40% (balance primarily myristic, palmitic, and stearic acids) |
application(s) | DNA extraction: suitable, RNA extraction: suitable, cell culture | mammalian: suitable, protein purification: suitable |
impurities | 3.0% water |
refractive index | n20/D 1.468 (lit.) |
CMC | 0.06 mM (20-25°C) |
acid number | 2.2 mg/g |
hydroxyl value | 96108 mg/g |
transition temp | cloud point 76 °C |
density | 1.095 g/mL at 25 °C (lit.) |
HLB | 16.7 |
suitability | complies for IR spectroscopy |
InChI | 1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3 |
InChI key | HMFKFHLTUCJZJO-UHFFFAOYSA-N |
Personal Protective Equipment | Eyeshields, Gloves |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | 527.0 °F - Pensky-Martens closed cup |
Flash Point(C) | 275 °C - Pensky-Martens closed cup |