Cytiva

IPG Buffers are ampholyte-containing buffer concentrates specifically formulated for use with Immobiline® DryStrip gels. Each IPG Buffer type produces more uniform conductivity along the Immobiline® DryStrip during focusing, resulting in wider latitude in run times. IPG Buffers also eliminate high background staining.
IPG buffers are supplied in 1-ml aliquots of water. The buffer is diluted 50- or 200-fold in the rehydration solution, depending on the first-dimension system and pH range of the strip.

For use with Immobiline® DryStrip gels to improve Protein solubility.
Using Immobiline® DryStrip gels together with matching IPG buffer gives high sample loading capacity, improves resolution, and increases the probability of detecting Low abundance Proteins.
Improved IPG buffer pH 3-10 and pH 3-10 NL offer improved resolution, sensitivity, and increased spot intensity as well as additional Protein spots in high molecular weight and basic pH areas.
Eliminates background staining.
Ensures optimal conditions for IEF separation.

Immobiline is a registered trademark of Cytiva

sterility	non-sterile
form	liquid
shelf life	Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
feature	wetted part: no
packaging	pkg of 1 mL
Торговая марка	Cytiva
color	Colorless to light yellow
pH range	4 - 7
solubility	cold water: soluble (Soluble in cold water and hot water)
storage temp.	2-8°C

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KTA™ start protein purification system GE29-0220-94

NA.55

KTA start is an easy-to-use chromatography system that automates manual purification procedures using for example HiTrap columns.

Easily purify your tagged proteins, antibodies, etc.
Gain insights from real-time monitoring
Evaluate and share your purification results
Entry level chromatography solution for the preparative purification of proteins in lab scale

available in Europe only

3D Insert is a trademark of 3D Biotek, LLC

description	2 buffer inlets
Торговая марка	Cytiva
parameter	(single wave length), 0.5 Mpa max. pressure (operating), 0.5 Mpa max. pressure (operating, sample pump), 0.5-5 mL/min flow rate
pathlength	2 mm , flow cell
viscosity	0.6-5 cP

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Lentil Lectin-Sepharose® 4B 25 mL GE17-0444-01

Lentil Lectin Sepharose® media is used for purification of a number of glycoproteins and other carbohydrate containing molecules. The lentil lectin ligand has more equal affinity for -D-mannose and -D-glucose compared to Con A.
Lentil lectin is a metalloprotein, containing Ca2+ and Mn2+, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars. Lentil Lectin Sepharose® 4B is a generally applicable group specific adsorbent and is routinely used in the preparation and purification of a number of glycoproteins and carbohydrate containing molecules.

Binds molecules that contain -D-mannose, -D-glucose, and sterically related residues
Compared to Con A, also for glycoprotein purification, Lentil Lectin distinguishes less sharply between glucosyl and mannosyl
Retains binding in 1% deoxycholate and is especially valuable for purification of detergent-solubilized membrane proteins
Lentil lectin is a metalloprotein, containing Ca2+ and Mn2+, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars

Sepharose is a registered trademark of Cytiva

shelf life	Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging	pkg of 25 mL
Торговая марка	Cytiva
storage condition	(20% Ehtanol, PBS pH 7)
matrix	4% agarose
particle size	45-165 µm
average diameter	90 µm
cleaning in place	3 - 12
working range	3 - 10
storage temp.	2-8°C

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Leukotriene C4/D4/E4, Biotrak™ EIA System GERPN224

NA.32

Leukotriene C4/D4/E4 Biotrak™ enzymeimmunoassay system from Cytiva has been specifically designed for research purposes. It combines the use of a Peroxidase labelled Leukotriene C4 conjugate, a peptido Leukotriene specific antiserum which can be immobilized on to pre-coated microplates, and a one pot stabilized substrate solution. This provides a rapid and sensitive non-isotopic method for the determination of Leukotriene C4/D4/E4 in the range 0.77 pg to 49.5 pg/well. Each pack contains sufficient material for 96 wells. This permits the construction of one standard curve and 39 unknowns in duplicate. The assay is based on the competition between unlabelled Leukotriene C4 and a fixed quantity of Peroxidase labelled Leukotriene C4 for a limited number of binding sites on a peptido leukotriene specific antibody.
With fixed amounts of antibody and Peroxidase labelled Leukotriene C4 the amount of Peroxidase labelled ligand bound by the antibody will be inversely proportional to the concentration of added unlabelled ligand. The Peroxidase ligand that is bound to the antibody is immobilized on to polystyrene microplate wells precoated with second antibody, as demonstrated in figure 1. Thus any unbound ligand can be removed from the well by simple washing procedures. The amount of Peroxidase labelled Leukotriene C4 bound to the antibody is determined by addition of Tetramethylbenzidine (TMB)/ Hydrogen Peroxide single pot substrate. The reaction is stopped by addition of an acid solution, and the resultant colour read at 450 nm in a microplate spectrophotometer. The concentration of unlabelled Leukotriene C4 in a sample is determined by interpolation from a standard curve.

High sensitivity ~ 0.5 pg/well for Leukotriene C4/D4, 10 pg/mL.
Nonisotopic.
Ready-to-use substrate.
Color-coded reagents.
Choice of protocol.

Biotrak is a trademark of Cytiva

shelf life	Please be aware this product may be shipped 30 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Торговая марка	Cytiva RPN224
packaging	pkg of 96 wells
shipped in	dry ice
storage temp.	20°C

signalword	Warning
hcodes	H226
pcodes	P210-P235-P241-P280-P303 + P361 + P353-P501
RIDADR	NONH for all modes of transport

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MabSelect SuRe™ LX GE17-5474-01

Novel, alkali-tolerant rProtein A ligand withstands rigorous CIP and sanitization procedures with 0.1 to 0.5 M NaOH.
Reduces the risk of product contamination and carryover
Improves performance and reduces overall costs
High dynamic binding capacity (DBC) for high titer cultures reduces process time and amount of medium used
Generic elution profile for different monoclonal antibodies enables platform approach to purification
High-flow agarose matrix allows the processing of large feed volumes

Free MabSelect SuRe Ligand
"MabSelect SuRe Ligand Restricted License" and "Cys-rProtein A Ligand Restricted License" are protected by the following patents and equivalent patents and patent applications in other countries: US 6,399,750 and EP 1123389. A free, non-transferable limited license to use this product for internal analytical purposes only accompanies the purchase of the product from a Cytiva company and its licensed distributors. Any other use will require a separate license from a Cytiva company.
MabSelect is a trademark of Cytiva
MabSelect SuRe is a trademark of Cytiva
SuRe is a trademark of Cytiva

shelf life	Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging	pack of 25 mL
Торговая марка	Cytiva
storage condition	(20% Ehtanol)
matrix	highly cross-linked agarose
avg. part. size	85 µm (dv is the median particle size of the cumulative volume distribution)
cleaning in place	2 - 13.7 (pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
working range	3 - 12 (pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
capacity	~60 mg binding capacity(human IgG/ml medium. Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 100 cm/h in a column with a bed height of 20 cm, i.e. residence time is 6.0 min.)
suitability	suitable for bioprocess medium
storage temp.	2-8°C

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MabSelect SuRe™ LX GE17-5474-02

Novel, alkali-tolerant rProtein A ligand withstands rigorous CIP and sanitization procedures with 0.1 to 0.5 M NaOH.
Reduces the risk of product contamination and carryover
Improves performance and reduces overall costs
High dynamic binding capacity (DBC) for high titer cultures reduces process time and amount of medium used
Generic elution profile for different monoclonal antibodies enables platform approach to purification
High-flow agarose matrix allows the processing of large feed volumes

shelf life	Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging	pack of 200 mL
Торговая марка	Cytiva
storage condition	(20% Ehtanol)
matrix	highly cross-linked agarose
avg. part. size	85 µm (dv is the median particle size of the cumulative volume distribution)
cleaning in place	2 - 13.7 (pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
working range	3 - 12 (pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
capacity	~60 mg binding capacity(human IgG/ml medium. Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 100 cm/h in a column with a bed height of 20 cm, i.e. residence time is 6.0 min.)
suitability	suitable for bioprocess medium
storage temp.	2-8°C

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MabSelect™ GE17-5199-01

MabSelect™ is a protein A affinity medium designed for capturing MAbs from large sample volumes.

MabSelect™ is a BioProcess affinity medium for capturing monoclonal antibodies (MAbs) from large sample volumes. MabSelect™ is developed for cost-effective high throughput processing of large amounts of feed. This is the reason why MabSelect™ has been designed-in to several new and second-generation processes for the production of therapeutic MAbs.
The medium is based on a highly cross-linked Agarose matrix with a recombinant Protein A ligand. This ligand is engineered to favor an oriented coupling resulting in an affinity medium with enhanced binding capacity for IgG. The hydrophilic nature of the base matrix ensures Low levels of non-specific binding leading to Low levels of host cell-derived impurities in the elution pool. MabSelect™ is optimized for high capacity, throughput and to deliver a product pool that is high in purity and yield.
The recombinant Protein A ligand is expressed in E. coli and is free of components of mammalian origin. The specificity of binding to the Fc region of IgG is similar to that of native Protein A giving a high purification factor in a single step. In addition, the high capacity and the novel base matrix make MabSelect™ an excellent choice for purification of MAbs at process scale.
MabSelect™ is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, MabSelect™ meets industrial demands with security of supply and comprehensive technical and regulatory support.

High-fLow Agarose matrix allows for high flow velocities at production scale enabling the processing of more than 10 000 L of feed in one working day.
Oriented coupling of the ligand and optimized matrix gives high dynamic binding capacity which reduces media volume requirements
Low non-specific binding resulting in Low levels of impurities in the product eluate pool
BioProcess medium supported for industrial applications and well-established in approved processes.

MabSelect is a trademark of Cytiva

shelf life	Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging	pack of 25 mL
Торговая марка	Cytiva
matrix	highly cross-linked agarose
avg. part. size	85 µm (dv is the median particle size of the cumulative volume distribution)
cleaning in place	2 - 12 (pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
working range	3 - 10
capacity	30 mg binding capacity (humanlgG/ml at 2.4 min residence time)(Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.)
suitability	suitable for bioprocess medium
storage temp.	2-8°C

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MabSelect™ PrismA GE17549802

MabSelect PrismA affinity chromatography resin has been improved with an optimized high-flow agarose base matrix and a genetically engineered protein A-derived ligand. This allows future demands in mAb purification to be met, including processing of many bispecific antibodies.

Enhanced dynamic binding capacity allows high mass throughput of processed mAb per resin volume unit.
Excellent alkaline stability enables efficient cleaning and sanitization using 0.5–1.0 M NaOH for improved process economy, bioburden control, and robustness.

The enhanced alkaline stability of MabSelect PrismA also provides opportunities for more robust mAb purification using periodic counter-current chromatography. The possibility for cleaning using 1 M NaOH increases bioburden control for continuous processes, which can be exposed to days or weeks of feed harvest.

MabSelect is a trademark of Cytiva

pictograms	GHS02,GHS02
signalword	WarningDanger
hcodes	H226 - H225
pcodes	P210 - P235 - P241 - P280 - P210 - P233 - P240 - P241 - P242 - P243 - P303 + P361 + P353 - P501
WGK Germany	WGK 1
Flash Point F	55.4 °F
Flash Point C	13 °C

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GE17549801
MabSelect™ PrismA GE17549801
General description
MabSelect PrismA affinity chromatography resin has been improved with an optimized high-flow agarose base matrix and a genetically engineered protein A-derived ligand. This allows future demands in mAb purification to be met, including processing of many bispecific antibodies.

Enhanced dynamic binding capacity allows high mass throughput of processed mAb per resin volume unit.
Excellent alkaline stability enables efficient cleaning and sanitization using 0.5–1.0 M NaOH for improved process economy, bioburden control, and robustness.

The enhanced alkaline stability of MabSelect PrismA also provides opportunities for more robust mAb purification using periodic counter-current chromatography. The possibility for cleaning using 1 M NaOH increases bioburden control for continuous processes, which can be exposed to days or weeks of feed harvest.
Legal Information
MabSelect is a trademark of Cytiva
Параметры

Торговая марка 17549801, Cytiva

Safety Information

pictograms GHS02

signalword Warning

hcodes H226

pcodes P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501

WGK Germany WGK 1

Flash Point F 55.4 °F

Flash Point C 13 °C
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MabSelect™ SuRe™ GE17-5438-01

Novel, alkali-tolerant rProtein A ligand withstands rigorous CIP and sanitization procedures with 0.1 to 0.5 M NaOH.
Reduces the risk of product contamination and carryover
Improves product quality and reduces overall costs
Novel ligand design gives enhanced protease resistance resulting in lower ligand leakage
Generic elution profile for different monoclonal antibodies enables platform approach to purification
Used in the commercial manufacture of monoclonal antibodies
High-flow agarose matrix allows the processing of large feed volumes

shelf life	Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging	pack of 25 mL
Торговая марка	Cytiva
storage condition	(20% Ehtanol)
matrix	highly cross-linked agarose
avg. part. size	85 µm (dv is the median particle size of the cumulative volume distribution)
cleaning in place	2 - 13.7 (pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
working range	3 - 12 (pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
capacity	30 mg binding capacity (humanlgG/ml at 2.4 min residence time)(Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.)
suitability	suitable for bioprocess medium
storage temp.	2-8°C

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MabSelect™ SuRe™ GE17-5438-02

Novel, alkali-tolerant rProtein A ligand withstands rigorous CIP and sanitization procedures with 0.1 to 0.5 M NaOH.
Reduces the risk of product contamination and carryover
Improves product quality and reduces overall costs
Novel ligand design gives enhanced protease resistance resulting in lower ligand leakage
Generic elution profile for different monoclonal antibodies enables platform approach to purification
Used in the commercial manufacture of monoclonal antibodies
High-flow agarose matrix allows the processing of large feed volumes

shelf life	Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging	pack of 200 mL
Торговая марка	Cytiva
storage condition	(20% Ehtanol)
matrix	highly cross-linked agarose
avg. part. size	85 µm (dv is the median particle size of the cumulative volume distribution)
cleaning in place	2 - 13.7 (pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
working range	3 - 12 (pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
capacity	30 mg binding capacity (humanlgG/ml at 2.4 min residence time)(Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.)
suitability	suitable for bioprocess medium
storage temp.	2-8°C

MabSelect™ Xtra has the same recombinant Protein A ligand as MabSelect™ but is based on a high-fLow Agarose base matrix with increased porosity and a slightly decreased particle size compared to MabSelect. This results in higher capacity—MabSelect™ Xtra has about 30% higher dynamic binding capacity for IgG than currently available Protein A chromatography media. In addition, MabSelect™ Xtra has a higher capacitiy for many Fc-fusion Proteins.
The medium provides a lower overall production cost due to the possibility of processing concentrated feedstocks in fewer batches.
MabSelect™ Xtra is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, MabSelect™ Xtra meets industrial demands with security of supply and comprehensive technical and regulatory support.

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MabSelect™ Xtra GE17-5269-02

Higher dynamic binding capacity than currently available Protein A chromatography media.
Improved process economics through reduced raw materials costs and/or reduced number of cycles
High purity capture due to high ligand selectivity and matrix hydrophilicity
Slightly lower pressure/fLow properties compared to MabSelect™ SuRe™ and MabSelect

MabSelect is a trademark of Cytiva
SuRe is a trademark of Cytiva

shelf life	Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging	pack of 200 mL
Торговая марка	Cytiva
storage condition	(20% Ehtanol)
matrix	highly cross-linked agarose
avg. part. size	75 (dv is the median particle size of the cumulative volume distribution)
cleaning in place	2 - 12 (pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
working range	3 - 10 (pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
capacity	30 mg binding capacity (humanlgG/ml at 2.4 min residence time)(Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.)
suitability	suitable for bioprocess medium
storage temp.	2-8°C

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MabSelect™ Xtra GE17-5269-07

MabSelect™ Xtra, with extra high dynamic binding capacity, is a member of the MabSelect™ family. Developed to meet the demands of increasing levels of expression in monoclonal antibody feedstocks.
MabSelect™ Xtra has the same recombinant Protein A ligand as MabSelect™ but is based on a high-flow agarose base matrix with increased porosity and a slightly decreased particle size compared to MabSelect. This results in higher capacity—MabSelect™ Xtra has about 30% higher dynamic binding capacity for IgG than currently available Protein A chromatography media.
In addition, MabSelect™ Xtra has a higher capacitiy for many Fc-fusion proteins.
The medium provides a lower overall production cost due to the possibility of processing concentrated feedstocks in fewer batches.
MabSelect™ Xtra is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, MabSelect™ Xtra meets industrial demands with security of supply and comprehensive technical and regulatory support.

Higher dynamic binding capacity than currently available Protein A chromatography media.
Improved process economics through reduced raw materials costs and/or reduced number of cycles
High purity capture due to high ligand selectivity and matrix hydrophilicity
Slightly lower pressure/flow properties compared to MabSelect™ SuRe™ and MabSelect

MabSelect is a trademark of Cytiva
SuRe is a trademark of Cytiva

shelf life	Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging	pack of 25 mL
Торговая марка	Cytiva
storage condition	(20% Ehtanol)
matrix	highly cross-linked agarose
avg. part. size	75 (dv is the median particle size of the cumulative volume distribution)
cleaning in place	2 - 12 (pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
working range	3 - 10 (pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
capacity	30 mg binding capacity (humanlgG/ml at 2.4 min residence time)(Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.)
suitability	suitable for bioprocess medium
storage temp.	2-8°C

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MAbtrap® Kit GE17-1128-01

MAbTrap® is an affinity chromatography kit, with everything required for small-scale IgG purification from ascites fluid, serum, and cell culture supernatants.

MAbTrap® Kit is an affinity chromatography kit for fast and effective purification of monoclonal and polyclonal IgG from ascites fluid, serum, and cell culture supernatants. MAbTrap® Kit contains everything necessary to perform a complete one step purification without using complicated equipment. It also eliminates time-consuming buffer preparation and column packing. The kit includes one HiTrap® column, prepacked with Protein G Sepharose® High Performance.

Convenient kit for purification of IgG from monoclonal and polyclonal material using a syringe in less than 15 min.
Kit contains one HiTrap® Protein G HP column prepacked with Protein G Sepharose® High Performance and enough buffer concentrate for 20 preparations with a syringe.
MAbTrap® Kit contains everything necessary to perform a complete one-step purification without using complex equipment.

HiTrap is a registered trademark of Cytiva
MAbTrap is a registered trademark of Cytiva
Sepharose is a registered trademark of Cytiva

shelf life	Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging	pkg of 1 kit
Торговая марка	Cytiva
storage condition	(20% Ethanol)
parameter	<4 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.), 42 psi
bed size	7 mm x 25 mm
bed volume	1 mL
column I.D.	7 mm
matrix	highly cross-linked 6% agarose
avg. part. size	34 µm
cleaning	2 - 9 (pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
working range	3 - 9 (pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
capacity	>1 mg binding capacity (lgG/column)(The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
storage temp.	2-8°C

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Matrix Metalloproteinase-2 (MMP-2) Biotrak™ Activity Assay System GERPN2631

NA.32

Matrix Metalloproteinase-2, Biotrak™ Assay.
Matrix metalloproteinase-2 (MMP-2), also known as gelatinase or type IV collagenase, is a 72 kDa protein. MMP-2 is a member of matrix metalloproteinase (MMP) family of enzymes. The basic structure of MMP2 contains a signal peptide domain that targets the enzyme for secretion, the pro-peptide domain, which is removed when the enzyme is activated and the catalytic site containing gelatin-binding domain.
Biotrak™ MMP-2, Activity Assay System provides a simple, specific and precise quantitative determination of active or pro MMP-2 in tissue culture supernatants, serum, plasma, urine, synovial fluid and tissue homogenates. Active MMP-2 may be measured in the range 0.75 to 12 ng/mL and the sensitivity of the assay is 0.5 ng/mL. A longer incubation protocol enables an assay range of 0.19 to 3 ng/mL with a sensitivity of 190 pg/mL, should higher sensitivity be required. The assay uses the pro form of a detection enzyme that can be activated by captured active MMP-2, into an active detection enzyme, through a single proteolytic event. The natural activation sequence in the pro detection enzyme has been replaced using Protein engineering, with an artificial sequence recognised by specific matrix metalloProteinases. MMP activated detection enzyme can then be measured using a specific chromogenic peptide substrate. Standards and samples are incubated in microplate wells precoated with anti-MMP-2 antibody. Any MMP-2 present will be bound to the wells, other components of the sample being removed by washing and aspiration. Either the endogenous levels of free active MMP-2, or the total levels of free MMP-2 in a sample, can be detected. In order to measure the total MMP-2 content, any bound MMP-2 in its pro form is activated using p-aminophenylmercuric acetate (APMA). The standard is pro MMP-2 which is activated in parallel for both types of sample. Active MMP-2 is detected without APMA treatment. The resultant colour is read at 405 nm in a microplate spectrophotometer. The concentration of active MMP-2 in a sample is determined by interpolation from a standard curve. Each pack contains reagents for 96 determinations. This allows the construction of a standard curve plus the measurement of 42 samples in duplicate. The Biotrak™ MMP-2 activity assay has been specifically designed for research purposes.

Biochem/physiol Actions

Matrix metalloproteinase-2 (MMP-2) cleaves gelatin, type IV, V, VII, X, and XI collagens, fibronectin, elastin, laminin, proteoglycans and a range of non-extracellular matrix (ECM) components. Both MMP-2 and MMP9 play an essential role in matrix degradation and they are implicated in the maintenance of neovascularization. MMP-2 activity is associated with human ovarian follicular development. Its expression is elevated in human myocardial infarction and dilated cardiomyopathy. Wound fluid from chronic leg ulcers show elevated expression of MMP2. Overexpression of active MMP-2 affects wound healing in chronic leg ulcers. MMP2 activity is inhibited by tissue inhibitor of metalloproteinase-2 (TIMP-2). Human follicular fluid MMP-2 level acts as a potential biomarker of oocyte maturation in in vitro fertilization and intracytoplasmic sperm injection (ICSI) cycles.

Specific for MMP-2.
Nonradioactive microplate-based assay.
Precise, accurate, quantitative measurement.
Applicable to complex biological samples.
Ready-to-use detection enzyme and substrate.
Equivalent sensitivity to ELISA.

Biotrak MMP-2 and TIMP-2 Human ELISAs
These products are manufactured by FUJI CHEMICAL INDUSTRIES, Ltd. (Japan).
Biotrak is a trademark of Cytiva

shelf life	Please be aware this product may be shipped 30 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging	pack of 1 ea
Торговая марка	Cytiva RPN2631
shipped in	dry ice
storage temp.	20°C

pictograms	GHS09
signalword	Danger
hcodes	H300 + H310 + H330 - H373 - H410
pcodes	P260 - P262 - P273 - P280 - P301 + P310 - P302 + P310 - P304 + P340 + P310 - P405 - P501
RIDADR	NONH for all modes of transport

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MBPTrap™ HP GE29-0486-41

MBPTrap HP columns prepacked with Dextrin Sepharose High Performance provide convenient, high binding capacity, affinity purification of recombinant proteins tagged with maltose binding protein (MBP).

Highly pure MBP-tagged recombinant proteins eluted in concentrated form and small volumes.
Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH.
Physiological conditions and mild elution preserve the activity of the target protein.
Convenient, time-saving operation and reproducible results

MBPTrap is a trademark of Cytiva

shelf life	Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging	pack of 1 mL
Торговая марка	Cytiva
storage condition	(20% Ehtanol)
parameter	<4 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.), 42 psi
bed size	7 mm x 25 mm
bed volume	1 mL
column I.D.	7 mm
size
avg. part. size	34 µm
cleaning	2 - 13
working range	>7
capacity	~7 mg binding capacity (MBP- Sal/ml medium Approx. 16 mg MBP- Gal/ml medium)(The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
storage temp.	2-8°C

NA 1993 / PGIII

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MBPTrap™ HP GE28-9187-78

MBPTrap is a trademark of Cytiva

shelf life	Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging	pack of 5 x 1 mL
Торговая марка	Cytiva
storage condition	(20% Ehtanol)
parameter	<4 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.), 42 psi
bed size	7 mm x 25 mm
bed volume	1 mL
column I.D.	7 mm
matrix	highly cross-linked 6% agarose
avg. part. size	34 µm
cleaning	2 - 13
working range	>7
capacity	~7 mg binding capacity (MBP- Sal/ml medium Approx. 16 mg MBP- Gal/ml medium)(The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
storage temp.	2-8°C

UN1170 - class 3 - PG 3 - Ethanol, solution

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MBPTrap™ HP GE28-9187-79

MBPTrap is a trademark of Cytiva

shelf life	Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging	pack of 1 x 5 mL
Торговая марка	Cytiva
storage condition	(20% Ehtanol)
parameter	<20 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.), 42 psi
bed size	16 mm x 25 mm
bed volume	5 mL
column I.D.	16 mm
matrix	highly cross-linked 6% agarose
avg. part. size	34 µm
cleaning	2 - 13
working range	>7
capacity	~7 mg binding capacity (MBP- Sal/ml medium Approx. 16 mg MBP- Gal/ml medium)(The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
storage temp.	2-8°C

pictograms	GHS02
signalword	Warning
hcodes	H226
pcodes	P210-P370 + P378
RIDADR	UN1170 - class 3 - PG 3 - Ethanol, solution

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MBPTrap™ HP GE28-9187-80

MBPTrap™ HP columns prepacked with Dextrin Sepharose® High Performance provide convenient, high binding capacity, affinity purification of recombinant proteins tagged with maltose binding protein (MBP).

HiTrap® columns prepacked with Dextrin Sepharose® High Performance medium.
Highly pure MBP-tagged recombinant proteins eluted in concentrated form and small volumes.
Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH.
Physiological conditions and mild elution preserve the activity of the target protein.
Convenient, time-saving operation and reproducible resultsLab Purification using the affinity to the MBP tag.

HiTrap is a registered trademark of Cytiva
MBPTrap is a trademark of Cytiva
Sepharose is a registered trademark of Cytiva

shelf life	Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging	pack of 5 x 5 mL
Торговая марка	Cytiva
storage condition	(20% Ehtanol)
parameter	<20 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.), 42 psi
bed size	16 mm x 25 mm
bed volume	5 mL
column I.D.	16 mm
matrix	highly cross-linked 6% agarose
avg. part. size	34 µm
cleaning	2 - 13
working range	>7
capacity	~7 mg binding capacity (MBP- Sal/ml medium Approx. 16 mg MBP- Gal/ml medium)(The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
storage temp.	2-8°C

pictograms	GHS02
signalword	Warning
hcodes	H226
pcodes	P210-P370 + P378
RIDADR	UN1170 - class 3 - PG 3 - Ethanol, solution

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Megaprime™ DNA Labelling System, dCTP GERPN1606

NA.31

Megaprime™ DNA Labeling System, dCTP (for use with labeled dCTP).

Megaprime™ DNA Labelling System, dCTP has been used to label probe PCR product.
Megaprime™ DNA Labeling Systems are used for rapid, random-prime labeling from as little as 25 ng substrate DNA. Each system includes the following reagents, sufficient for 30 or 60 reactions (depending on product ordered): primer solution, labeling buffer, reaction buffer, enzyme, standard DNA solution, and carrier DNA solution.

For labeling of DNA probes to specific activities > 1 x 109 dpm/µg in 10 min.
Features cloned Klenow fragment and random nonamers in the random-prime labeling technique.
No need to remove unincorporated nucleotides before hybridization.
Protocols for labeling directly from Low melting point Agarose.

Megaprime is a trademark of Cytiva

usage	sufficient for 30 reactions
shelf life	Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Торговая марка	Cytiva RPN1606
shipped in	dry ice
storage temp.	20°C

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Megaprime™ DNA Labelling System, dCTP GERPN1607

NA.31

Megaprime™ DNA Labeling System, dCTP (for use with labeled dCTP).

Megaprime™ DNA Labeling Systems are used for rapid, random-prime labeling from as little as 25 ng substrate DNA. Each system includes the following reagents, sufficient for 30 or 60 reactions (depending on product ordered): primer solution, labeling buffer, reaction buffer, enzyme, standard DNA solution, and carrier DNA solution.

For labeling of DNA probes to specific activities > 1 x 109 dpm/µg in 10 min.
Features cloned Klenow fragment and random nonamers in the random-prime labeling technique.
No need to remove unincorporated nucleotides before hybridization.
Protocols for labeling directly from Low melting point Agarose.

Megaprime is a trademark of Cytiva

usage	sufficient for 60 reactions
shelf life	Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Торговая марка	Cytiva RPN1607
shipped in	dry ice
storage temp.	20°C

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Megaprime™ DNA Labelling System, dNTP GERPN1604

NA.31

Megaprime™ DNA Labeling System, dNTP (for use with any radioactively labeled nucleotide).

For labeling of DNA probes to specific activities > 1 x 109 dpm/µg in 10 min.
Features cloned Klenow fragment and random nonamers in the random-prime labeling technique.
No need to remove unincorporated nucleotides before hybridization.
Protocols for labeling directly from Low melting point Agarose.

Megaprime is a trademark of Cytiva

usage	sufficient for 30 reactions
shelf life	Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Торговая марка	Cytiva RPN1604
shipped in	dry ice
storage temp.	20°C

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Methylenebis Acrylamide GE17-1304-02

PlusOne N,N′-Methylenebisacrylamide is a general cross-linker used with acrylamide for PAGE techniques. It is available as a dry powder and a premixed solution.

form	solid
shelf life	Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
feature	wetted part: no
packaging	pack of 100 g
Торговая марка	Cytiva
color	White
pH range	5.5 - 7.0
solubility	acetone: soluble (Slightly soluble in acetone), cold water: soluble (Soluble in cold water and hot water)
storage temp.	2-8°C

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Microarray Hybridisation Buffer Version 2 GERPK0325

shelf life	Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging	pack of 1 ea
Торговая марка	Cytiva RPK0325
shipped in	dry ice
storage temp.	−20°C

MicroSpin™ columns are designed for the rapid purification of DNA in conjunction with Sephadex® G-25 DNA Grade for use in a wide range of applications, including desalting, buffer exchange, and removal of unincorporated nucleotides from end-labelled oligonucleotides. They can be used for any DNA greater than 10 bases in length and are therefore highly suitable for the purification of oligonucleotides or very small DNA fragments following synthesis or a labeling reaction.

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Microspin™ Columns GE27-3565-01

For rapid buffer exchange/desalting of PCR products and other DNAs in a volume of 10 to 100 µL using spin-column chromatography and Sephadex® G-25 DNA Grade (not included).
Excellent for rapid purification of newly synthesized oligonucleotides > 10-mers in 100 to 150 ?l of deprotection solution using spin-column chromatography.
Flexible for your experiments, allowing differing amounts of Sephadex.
Can also be used for desalting/buffer exchange of DNA and removal of unincorporated radionucleotides from end-labeled oligonucleotides (at least 10 bases in length) in a volume of 10 to 100 µL.

MicroSpin is a trademark of Cytiva
Sephadex is a registered trademark of Cytiva

shelf life	Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging	pack of 1 pieces
Торговая марка	Cytiva

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Microspin™ G-25 Columns GE27-5325-01

MicroSpin™ G-25 columns are designed for the rapid purification of DNA for use in a wide range of applications, including desalting, buffer exchange, and removal of unincorporated nucleotides from end-labelled oligonucleotides.
MicroSpin™ G-25 columns can be used for any DNA greater than 10 bases in length and are therefore an excellent choice for the purification of oligonucleotides or very small DNA fragments following synthesis or a labelling reaction. They will not remove or denature enzyme.

Store at 20 to 25 °C. (Double distilled water containing 0.05% Kathon CG/ICP Biocide as preservative)

For rapid buffer exchange/desalting of PCR products and other DNAs in a volume of 10 to 100 µl using spin-column chromatography.
Excellent for rapid purification of newly synthesized oligonucleotides >10-mers in 100 to 150 µl of deprotection solution using spin-column chromatography.
Convenient: Prepacked with Sephadex® G-25 DNA Grade and pre-equilibrated in distilled water containing 0.05% Kathon CG/ICP Biocide.
Ready to use: Requires less than 4 min from sample application to collection of purified product.
Tested in nickase, single and double-stranded exonuclease and RNase assays.
Can also be used for desalting/buffer exchange of DNA and removal of unincorporated radionucleotides from end-labeled oligonucleotides (at least 10 bases in length) in a volume of 10 to 100 µl.

Storage and Stability

MicroSpin is a trademark of Cytiva
Sephadex is a registered trademark of Cytiva

shelf life	Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Торговая марка	Cytiva
packaging	pkg of 50 columns
particle size	20-80 µm (dry)
working range	2 - 13

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Microspin™ G-50 Columns GE27-5330-01

MicroSpin™ G-50 columns are designed for the rapid purification of DNA for use in a wide range of applications, including desalting, buffer exchange, removal of dye terminators from cycle sequencing reactions and removal of labelled nucleotides from DNA labelling reactions. Good product yield and purity is obtained with sample volumes from 12-50 ul. They are suitable for any DNA greater than 20 bases in length. They will not remove or denature enzyme.

Store at 20 to 25 °C. (TE buffer (10 mM Tris/HCl, 1mM EDTA) containing 0.05% Kathon CG/ICP Biocide as preservative.)

For rapid purification of labeled DNA (>20 bases) from unincorporated labeled nucleotides in a volume of 25 to 50 µ using spin-column chromatography.
Columns are prepacked with Sephadex® G-50 Grade pre-equilibrated in TE buffer with 0.05% Kathon CG/ICP Biocide.
Product recovery will be at least 10% lower than that from ProbeQuant™ G-50 Micro Columns.
Can also be used for buffer exchange/desalting of enzymatic reactions (10 to 100 µ) following heat denaturation and phenol extraction.

Storage and Stability

MicroSpin is a trademark of Cytiva
ProbeQuant is a trademark of Cytiva
Sephadex is a registered trademark of Cytiva

shelf life	Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging	pack of 50 columns
Торговая марка	Cytiva
particle size	50-150 µm (dry)

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Microspin™ G-50 Columns GE27-5330-02

Store at 20 to 25 °C. (TE buffer (10 mM Tris/HCl, 1mM EDTA) containing 0.05% Kathon CG/ICP Biocide as preservative.)

For rapid purification of labeled DNA (>20 bases) from unincorporated labeled nucleotides in a volume of 25 to 50 µ using spin-column chromatography.
Columns are prepacked with Sephadex® G-50 Grade pre-equilibrated in TE buffer with 0.05% Kathon CG/ICP Biocide.
Product recovery will be at least 10% lower than that from ProbeQuant™ G-50 Micro Columns.
Can also be used for buffer exchange/desalting of enzymatic reactions (10 to 100 µ) following heat denaturation and phenol extraction.

Storage and Stability

MicroSpin is a trademark of Cytiva
ProbeQuant is a trademark of Cytiva
Sephadex is a registered trademark of Cytiva

shelf life	Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging	pack of 250 columns
Торговая марка	Cytiva
particle size	50-150 µm (dry)

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Microspin™ S-200 HR GE27-5120-01

MicroSpin™ columns are designed for the rapid purification of nucleic acids for use in a wide range of applications, including desalting, buffer exchange and removal of primers.

Good product yield and purity is obtained with sample volumes from 25-100 µL, and from nanogram to milligram quantities of DNA.

Convenient: Prepacked with Sephacryl® S-400 HR pre-equilibriated in TE buffer.
Ready to use: Requires less than 4 min from sample application to collection of purified product.
Tested in nickase, single- and double-stranded exonuclease and RNase assays.

For rapid purification of labeled single-stranded or double-stranded DNA fragments > 50 bases in length in a volume of 51 to 100 µL using spin-column chromatography.

MicroSpin is a trademark of Cytiva
Sephacryl is a registered trademark of Cytiva

shelf life	Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging	pack of 50 columns
Торговая марка	Cytiva
storage condition	(20% Ethanol), (TE buffer (10 mM Tris/HCl, 1mM EDTA, pH 7.6))
matrix	allyl dextran and N, N’-methylenebisacrylamide
particle size	25-75 µm
avg. part. size	50 µm
cleaning	2 - 13
working range	3 - 11
suitability	suitable for bioprocess medium
storage temp.	2-8°C

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Microspin™ S-300 HR GE27-5130-01

MicroSpin™ columns are designed for the rapid purification of nucleic acids for use in a wide range of applications, including desalting, buffer exchange and removal of primers.

illustra™ MicroSpin™ S-300 HR Columns: For rapid purification of PCR products (>100 bp) from unincorporated primers (<20-mers) and nucleotides using spin-column chromatography.

Accommodates 25 to 50 µL for post-PCR cleanup prior to sequencing.
Useful for purification of alkaline-denatured plasmid DNA prior to sequencing.
Convenient: Prepacked with Sephacryl® S-300 HR pre-equilibrated in TE buffer.
Ready to use: Requires less than 4 min from sample application to collection of purified product.
Tested in nickase, single- and double-stranded exonuclease and RNase assays.

MicroSpin is a trademark of Cytiva
Sephacryl is a registered trademark of Cytiva
illustra is a trademark of Cytiva

shelf life	Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging	pack of 50 columns
Торговая марка	Cytiva
storage condition	(20% Ethanol), (TE buffer (10 mM Tris/HCl, 1mM EDTA, pH 7.6))
matrix	allyl dextran and N, N’-methylenebisacrylamide
particle size	25-75 µm
avg. part. size	50 µm
cleaning	2 - 13
working range	3 - 11
suitability	suitable for bioprocess medium
storage temp.	2-8°C

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Microspin™ S-400 HR GE27-5140-01

MicroSpin™ columns are designed for the rapid purification of nucleic acids for use in a wide range of applications, including desalting, buffer exchange and removal of primers. Good product yield and purity is obtained with sample volumes from 25-100 µl, and from nanogram to milligram quantities of DNA.

illustra™ MicroSpin™ S-400 HR Columns: For rapid purification of PCR products (>200 bp) from unincorporated primers (<32-mers) and nucleotides using spin-column chromatography. - Accommodates 25 to 50 µ for post-PCR cleanup prior to cloning or a second amplification reaction or 51 to 100 µ for all other applications.
Convenient: Prepacked with Sephacryl® S-400 HR pre-equilibriated in TE buffer.
Ready to use: Requires less than 4 min from sample application to collection of purified product.
Tested in nickase, single- and double-stranded exonuclease and RNase assays.

MicroSpin is a trademark of Cytiva
Sephacryl is a registered trademark of Cytiva
illustra is a trademark of Cytiva

shelf life	Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging	pack of 50 columns
Торговая марка	Cytiva
storage condition	(20% Ethanol), (TE buffer (10 mM Tris/HCl, 1mM EDTA, pH 7.6))
matrix	allyl dextran and N, N’-methylenebisacrylamide
particle size	25-75 µm
avg. part. size	50 µm
cleaning	2 - 13
working range	3 - 11
suitability	suitable for bioprocess medium
storage temp.	2-8°C

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GE28-9232-44

MidiSpin Adapter GE28-9232-44

NACRES: NA.56

Параметры

feature wetted part: no

packaging pack of 10 pieces

Торговая марка Cytiva

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Mini Dialysis Kit, 1 kDa GE80-6483-75

The disposable tubes in Mini Dialysis Kit offer a simple solution to the handling problems of small volume dialysis. The dialysis tubes in the kit each have a cap that incorporates a small disk of dialysis membrane.

Dialyzing small samples can be difficult and inefficient when the sample must be transferred into and out of dialysis bags and centrifuge tubes. The disposable tubes in Mini Dialysis Kit offer a simple solution to the handling problems of small volume dialysis.
The dialysis tubes in the kit each have a cap that incorporates a small disk of dialysis membrane. Samples are easily pipetted into and removed from the conical bottom of the tube. The capped tube is inserted into a float and then, with the cap and membrane down, placed in a stirred beaker containing the desired dialysis solution.
Following dialysis, the tube is centrifuged briefly. This brings the entire contents of the tube back to the conical bottom for maximum recovery. The dialyzed sample can be stored in the same tube by simply replacing the dialysis cap with a standard cap.

Designed for efficient dialysis of small sample volumes.
Uses disposable dialysis tubes with dialysis membrane incorporated into caps.
Conical tube bottom maximizes sample recovery

shelf life	Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
feature	wetted part: no
packaging	pack of 250 μL
Торговая марка	Cytiva
storage temp.	2-8°C

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Mini Dialysis Kit, 1 kDa GE80-6483-94

Designed for efficient dialysis of small sample volumes.
Uses disposable dialysis tubes with dialysis membrane incorporated into caps.
Conical tube bottom maximizes sample recovery

shelf life	Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
feature	wetted part: no
packaging	pack of 2 mL
Торговая марка	Cytiva
storage temp.	2-8°C

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Mini Dialysis Kit, 8 kDa GE80-6484-13

Designed for efficient dialysis of small sample volumes.
Uses disposable dialysis tubes with dialysis membrane incorporated into caps.
Conical tube bottom maximizes sample recovery

shelf life	Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
feature	wetted part: no
packaging	pack of 250 μL
Торговая марка	Cytiva
storage temp.	2-8°C

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Mini Dialysis Kit, 8 kDa GE80-6484-32

Designed for efficient dialysis of small sample volumes.
Uses disposable dialysis tubes with dialysis membrane incorporated into caps.
Conical tube bottom maximizes sample recovery

shelf life	Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
feature	wetted part: no
packaging	pack of 2 mL
Торговая марка	Cytiva
storage temp.	2-8°C