SPB (succinimidyl-[4-(psoralen-8-yloxy)]butyrate) 803545-50MG

Empirical Formula (Hill Notation):	C19H15NO8
Molecular Weight:	385.32
PubChem Substance ID:	329768817
NACRES:	NA.22

SPB is an amine-reactive and photoreactive heterobifunctional crosslinking reagent. The photoreactive psoralen group provides much greater covalent insertion yields than phenyl azide-containing compounds, which makes this reagent a superior alternative to typical photoreactive NHS-ester crosslinkers. The psoralen tricyclic planar ring system intercalates into double-stranded, and to a lesser extent, single-stranded, DNA, RNA, PCR products, cDNA or oligonucleotides. It also can be used with agarose gel-purified oligonucleotides because the presence of agarose does not interfere with the coupling reaction. The nucleic acid labeling site of the psoralen group (the 5,6 double bond in thymine residues) does not interfere with subsequent hybridization reactions, creating a highly-sensitive direct labeling alternative to radioactive probes.
Features and Benefits

• Reactive groups: NHS ester and psoralen
• Reactive toward: amino groups and thymine
• DNA intercalating agent and nucleic acid probe1,2
• Reagent for photo-immobilization to microplates3
• Melting point: 177-178°C

Caution
This product is sensitive to moisture. The vial is packaged in a resealable bag with a desiccant to reduce exposure to moisture. After cold storage, equilibrate the vial to room temperature before opening to reduce condensation inside the vial. Make fresh solutions. Storage of stock solutions is not recommended. After use, return the vial to the resealable bag. Close the bag and store the product at the recommended temperature.

form	powder
Quality Level	100,
mol wt	385.32
reaction suitability	reagent type: cross-linking reagent
storage condition	desiccated
solubility	DMSO or DMF: soluble
shipped in	ambient
storage temp.	2-8°C
SMILES string	O=C1CCC(N1OC(CCCOC2=C(OC(C=C3)=O)C3=CC4=C2OC=C4)=O)=O
InChI	1S/C19H15NO8/c21-13-4-5-14(22)20(13)28-16(24)2-1-8-25-19-17-12(7-9-26-17)10-11-3-6-15(23)27-18(11)19/h3,6-7,9-10H,1-2,4-5,8H2
InChI key	ZOMXGWWLJXRQKZ-UHFFFAOYSA-N

RIDADR

NONH for all modes of transport