eSpCas9 Protein

NACRES:

NA.51

Кат. номер

ESPCAS9PRO-250UG-P

ESPCAS9PRO-50UG-PW

ESPCAS9PRO-50UG

ESPCAS9PRO-4X250UG

ESPCAS9PRO-250UG

ESPCAS9PRO-2X250UG

Recombinant eSpCas9 protein from Streptococcus pyogenes (~160 KD) is a ready-to-use reagent for genome engineering experiments. When combined with target-specific guide RNAs, eSpCas9 protein will act as a targeted nuclease suitable for transfection of cell cultures and for the accelerated development of genetically-modified animals via one-cell embryo injection.
Functional Genomics/Target Validation/Genome Editing

• Enhanced specificity compared to wild type Cas9
• Highly active
• Ready-to-inject/transfect

pkg of 50 µg
pkg of 250 µg
Each kit consists of:

• one vial of eSpCas9 recombinant protein
• one vial containing 1 mL of 1x dilution buffer
• one vial containing 1 mL of nuclease-free water with glycerol

Newly engineered eSpCas9 enables the efficient targeted gene editing of established CRISPR systems with the benefit of reduced off-target effects. Point mutations in the chromosome-binding motif of SpCas9, as described by Slaymaker, et al., provide higher on-target fidelity without loss of cleavage efficiency.
Lyophilized S. pyogenes eSpCas9 protein should be resuspended in the Reconstitution solution provided to desired concentration for storage. Gently tap tube to completely dissolve lyophilized powder, incubate for 10 minutes on ice, and spin tube to bring material to bottom of tube.
Use our CRISPR Selection Tool to order gRNA
Check out our other MISSION® Cas9 Proteins at SigmaAldrich.com/CRISPRproteins
CRISPR Use License Agreement
MISSION is a registered trademark of Sigma-Aldrich Co. LLC

Quality Level	300
recombinant	expressed in E. coli
assay	95% (SDS-PAGE)
form	lyophilized powder
packaging	pkg of 1 kit (3 components)
application(s)	CRISPR: suitable
shipped in	ambient
storage temp.	20°C

RIDADR	NONH for all modes of transport
Flash Point F	Not applicable
Flash Point C	Not applicable