CompoZr® Custom Zinc Finger Nuclease (ZFN)

Кат. номер

CSTZFN2

CSTZFN-1KT

CSTZFNY2

CSTZFN

Zinc Finger Nucleases (ZFNs) are a class of engineered DNA-binding proteins that facilitate targeted editing of the genome by creating double-strand breaks in DNA at user-specified locations. Double-strand breaks are important for site-specific mutagenesis in that they stimulate the cells natural DNA-repair processes, namely homologous recombination and Non-Homologous End Joining (NHEJ). By implementing established, field-proven methods, these processes can be harnessed to generate precisely targeted genomic edits, resulting in cell lines with precise and heritable gene deletions, integrations or modifications.
The CompoZr Custom ZFN service can be used to target genes from a variety of organisms. Validation of the custom ZFNs is completed in a proxy cell line for human, mouse, rat and hamster (CHO) projects. In this assay, the candidate ZFNs are delivered to the proxy cell line and activity is identified and measured. Activity is measured by amplifying over the ZFN target site and then using a nuclease mismatch enzyme to cut DNA strands that have been modified. ZFN efficiency can be measured based on the densitometry results of this assay. CompoZr Custom ZFN Projects for human, mouse, rat, and hamster (CHO) will have a production timeline of 10 weeks after the ZFN design is approved.
Functional Genomics/Target Validation

• Creation of gene knockouts in multiple cell lines
• Complete knockout of genes not amenable to RNAi

Cell-based Screening

• Creation of knock-in cell lines with promoters, fusion tags or reporters integrated into endogenous genes

Cell Line Optimization

• Creation of cell lines that produce higher yields of proteins or antibodies

Features and Benefits

• Rapid design, assembly, and validation of a ZFN pair targeting your gene of interest
• Rapid and permanent disruption of, or integration into, any genomic loci
• Mutations made are permanent and heritable
• Works in a variety of mammalian somatic cell types
• Edits induced through a single transfection experiment
• Knockout or knock-in cell lines in as little as two months
• Single or biallelic edits occur in 1-20% of clone population
• No antibiotic selection required for screening

Components

• Best Performing ZFN Pair
• 10 Aliquots of Ready-to-Deliver ZFN Pair in mRNA form
• ZFN Pair in Plasmid Form
• Forward and Reverse Primers that allow for determination of rate of mutation and for screening of individual clones
• Positive Control DNA
• Used to determine a baseline cutting efficiency

To have a ZFN made targeting your gene of interest, please inquire about our
Custom ZFN Service offering HERE,
All Zinc Finger Nucleases are sold under license from Sangamo Biosciences. CompoZr is a registered trademark of Sigma-Aldrich Co. LLC

Quality Level	200,
product line	CompoZr®
shipped in	dry ice
storage temp.	70°C

RIDADR	UN 3316 9
Flash Point F	Not applicable
Flash Point C	Not applicable