Cloning & Expression
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pTAC-MAT-Tag®-2 Expression Vector E5405-10UG
NACRES: NA.56 ApplicationCytoplasmic expression of C-terminal MAT-Tag® fusion proteins under control of the tac promoter.Other NotesSupplied with a pTAC-MAT-2+BAP Control Vector.
Browse additional application references in our FLAG® Literature, portal.Legal InformationFLAG is a registered trademark of Sigma-Aldrich Co. LLC
MAT-Tag is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200, shipped in dry ice storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Puromycin Selection Vector Set PP2372-1KT
NACRES: NA.85 General descriptionMolecular cloning often benefits from optimizing the vector used for expression.
This pack allows you to determine the optimum expression configuration for puromycin resistance in your mammalian cell experimental system. The puromycin resistance gene is provided under regulatory control of four different promoters, and an IRES, and each plasmid can be further to modified to include your gene of interest if required.This plasmid set has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI. The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site. Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Transcription Termination: These plasmids contains three alternative transcription terminators for mammalian bacterial and bacteriophage (T7) expression. This means that only the promoter needs to be changed to alter the expression system you are using. We sell multiple promoters that can be used in each of these systems. The presence of each terminator does not reduce expression in the alternative systems.
Sequence
For Genebank sequence, FASTA sequence, Quick-reference Plasmid Map, and Full Plasmid Map, see the individual vector product page for links.
Analysis NoteOther NotesLooking for more vector options to move your experiments forward faster? Consider a custom cloning vector designed and built by Oxford Genetics™. Find out more at Oxford Genetics, - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications.Legal InformationThese plasmids are sold free of reach-through rights and can be used to make commercial products. However the plasmids themselves (or derivatives) cannot be sold.
Oxford Genetics is a trademark of Oxford Genetics LtdПараметрыform buffered aqueous solution Origin of replication pUC (500 copies) Promoter Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalianbacteria selection kanamycin shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Rat IgG Vector Set PP2411-1KT
NACRES: NA.85 General descriptionMolecular cloning often benefits from optimizing the vector used for expression.
Rat IgG vector set enables you to engineer monoclonal expression of your chosen rat antibody. The plasmids encode the constant regions of rat IgG1 heavy chain and of both lambda and kappa light chains, providing a framework for you to insert the variable regions of your choice.This plasmid set has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI. The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site. Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Transcription Termination: Plasmids for molecular cloning contains three alternative transcription terminators for mammalian bacterial and bacteriophage (T7) expression. This means that only the promoter needs to be changed to alter the expression system you are using. We sell multiple promoters that can be used in each of these systems. The presence of each terminator does not reduce expression in the alternative systems.
Sequence
For Genebank sequence, FASTA sequence, Quick-reference Plasmid Map, and Full Plasmid Map, see the individual vector product page for links.
Analysis NoteOther NotesLooking for more vector options to move your experiments forward faster? Consider a custom cloning vector designed and built by Oxford Genetics™. Find out more at Oxford Genetics, - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications.Legal InformationThese plasmids are sold free of reach-through rights and can be used to make commercial products. However the plasmids themselves (or derivatives) cannot be sold.
Oxford Genetics is a trademark of Oxford Genetics LtdПараметрыform buffered aqueous solution Origin of replication pUC (500 copies) Promoter Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalianbacteria selection kanamycin shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Rosetta 2 Competent Cell Set - Novagen
Кат. номер 71405-3 71405-3CN General descriptionRosetta 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for 7 rare codones (AGA, AGG, AUA, CUA, GGA, CCC, and CGG) on a compatible chloramphenicol-resistant plasmid. The tRNA genes are driven by their native promoters.
DE3 indicates that the host is a lysogen of DE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
Genotypes:
Rosetta 2
F-ompT hsdSB(rB- mB-) gal dcm pRARE2 (CamR)
Rosetta 2(DE3)
F-ompT hsdSB(rB- mB-) gal dcm (DE3) pRARE2 (CamR)
Rosetta 2(DE3)pLysS
F-ompT hsdSB(rB- mB-) gal dcm (DE3) pLysSRARE2 (CamR)
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Components
•2 x 0.2 mlRosetta 2 Competent Cells
•2 x 0.2 mlRosetta 2(DE3) Competent Cells
•2 x 0.2 mlRosetta 2(DE3)pLysS Competent Cells
•4 x 2 mlSOC Medium
•2 ngTest Plasmid
Warning
Toxicity: Multiple Toxicity Values, refer to MSDS (O)Legal InformationNOVAGEN is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions = -70C Quality Level 100, Торговая марка Novagen® storage condition OK to freeze storage temp. 70°C Узнать цену -
SIG10 5a Chemically Competent Cells CMC0007-20X40UL
NACRES: NA.85 General descriptionThe SIG10 5a Chemically Competent Cells share the most useful genetic elements of standard cloning strains like DH5™ and directly replace them in cloning protocols.
They provide the performance researchers need with ease of use as only common laboratory equipment is required and provide solutions for a wide range of applications at economical prices.
Genotype
fhuA2(argF-lacZ)U169 phoA glnV44 80 (lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17Features and Benefits- Direct replacements for standard cloning strains like DH5™, DH10B™, JM109, TOP10, XL1-Blue, etc.
- Optimized genetics for high yields: endonuclease and recombination minus
- Blue - white screening
Components- SIG10 5a chemically competent cells
- pUC 19 transformation control DNA
- recovery medium for cloning
Legal InformationDH10B is a trademark of Life Technologies
DH5α is a trademark of Invitrogen Corp.Параметрыgrade for molecular biology form buffered aqueous solution cell transformation competent cell type: chemically competent
transformation efficiency: 1 x 108 cfu/µgshipped in dry ice storage temp. 70°C
Safety InformationRIDADR UN 3245 9 Узнать цену -
SIG10 Chemically Competent Cells
NACRES: NA.85
Кат. номер CMC0001-1EA CMC0001-20X160UL CMC0001-4X40UL CMC0001-20X40UL CMC0001-40X40UL CMC0001-20X80UL General descriptionThe SIG10 Chemically Competent Cells are a derivative of Escherichia coli that have been optimized for high transformation efficiency.
These cells are ideal for cloning and propagation of plasmid, cosmid, or fosmid clones and are highly efficient for routine cloning applications.
They share the most useful genetic elements of standard cloning strains like DH5™ DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols.
They are are provided in 40 µL, 80 µL and 160 µL aliquots, each being sufficient for one, two and four transformations respectively.
The cells have a transformation efficiency of >1 x 109 cfu/µg
The 96-well format are provided in aliquots of 20 µL per well and have a transformation efficiency of >1 x 108 cfu/µg.
Genotype
F- mcrA (mrr-hsdRMS-mcrBC) endA1 recA1 80dlacZM15 lacX74 araD139 (ara,leu)7697 galU galK rpsL nupG - tonAApplicationSuitable for bacterial transformations to recover high quality plasmid DNAFeatures and Benefits- share the most useful genetic elements of standard cloning strains like DH5™ DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols.
- ensures recovery of stable and high quality plasmid DNA.
- high transformation efficiency
- provide the performance researchers need with ease of use.
- provide solutions for a wide range of applications at economical prices.
- Blue - white screening
Components- SIG10 chemically competent cells
- pUC 19 transformation control DNA at 10 pg/µL
- recovery medium for cloning
PrincipleThe SIG10 cells contains the inactive mcr and mrr alleles, allowing methylated genomic DNA isolated directly from mammalian or plant cells to be cloned without deletions or rearrangements.
This strain also carries the recA1 and endA1 mutations. The recA1 genotype provides minimized recombination and aids in plasmid stability while endA1 provides for high quality plasmid DNA preparation.
They are bacteriophage T1-resistant (tonA mutation) and also resistant to streptomycin by virtue of rpsL mutation.Legal InformationDH10B is a trademark of Life Technologies
DH5α is a trademark of Invitrogen Corp.Параметрыgrade for molecular biology form buffered aqueous solution cell transformation competent cell type: chemically competent
transformation efficiency: 1 x 109 cfu/µgshipped in dry ice storage temp. 70°C
Safety InformationRIDADR UN 3245 9 Узнать цену -
SIG10 HIGH Electrocompetent Cells
NACRES: NA.85
Кат. номер CMC0003-20X25UL CMC0003-20X50UL CMC0003-20X100UL CMC0003-4X25UL CMC0003-40X25UL General descriptionThe SIG10 HIGH Electrocompetent Cells are high efficiency cells with a substantially lower cost per reaction and are the most economical choice for standard cloning and library construction.
Genotype
F- mcrA (mrr-hsdRMS-mcrBC) endA1 recA1 80dlacZM15 lacX74 araD139 (ara,leu)7697galU galK rpsL nupG - tonA (StrR)
Features and Benefits- share the most useful genetic elements of standard cloning strains like DH5a, DH10B, JM109, TOP10, etc. and directly replace them in cloning protocols.
- incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields and reliability.
- provide solutions for a wide range of applications at economical prices.
- Blue - white screening
Components- SIG10 HIGH electrocompetent cells
- pUC 19 transformation control DNA
- recovery medium for cloning
Параметрыgrade for molecular biology form buffered aqueous solution cell transformation competent cell type: electrocompetent
transformation efficiency: 5 x 109 cfu/µgshipped in dry ice storage temp. 70°C
Safety InformationRIDADR UN 3245 9 Узнать цену -
SIG10 MAX Electrocompetent Cells CMC0004-20X25UL
NACRES: NA.85 General descriptionThe SIG10 MAX Electrocompetent Cells provide large numbers of transformants from hard-to-clone fragments or limited DNA at a lower price.
Genotype
F- mcrA (mrr-hsdRMS-mcrBC) endA1 recA1 80dlacZM15 lacX74 araD139 (ara,leu)7697galU galK rpsL nupG - tonA (StrR)Features and Benefits- share the most useful genetic elements of standard cloning strains like DH5a, DH10B, JM109, TOP10, etc. and directly replace them in cloning protocols.
- incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields and reliability.
- provide solutions for a wide range of applications at economical prices.
- Blue - white screening
Components- SIG10 MAX electrocompetent cells
- pUC 19 transformation control DNA
- recovery medium for cloning
Параметрыgrade for molecular biology form buffered aqueous suspension cell transformation competent cell type: electrocompetent
transformation efficiency: 2 x 1010 cfu/µgshipped in dry ice storage temp. 70°C
Safety InformationRIDADR UN 3245 9 Узнать цену -
SIG10 ULTRA Electrocompetent Cells
NACRES: NA.85
Кат. номер CMC0006-20X25UL CMC0006-40X25UL CMC0006-20X50UL CMC0006-20X100UL CMC0006-4X25UL General descriptionThe SIG10 ULTRA Electrocompetent Cells have the highest transformation efficiency available. They are to be used for the most demanding cloning situations, such as construction of large, high complexity libraries or cloning difficult targets, which require the greatest number of transformants possible.
Genotype
F- mcrA (mrr-hsdRMS-mcrBC) endA1 recA1 80dlacZM15 lacX74 araD139 (ara,leu)7697galU galK rpsL nupG - tonA (StrR)
Features and Benefits- share the most useful genetic elements of standard cloning strains like DH5™ DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols.
- provide the performance researchers need with ease of use.
- provide solutions for a wide range of applications at economical prices.
- Blue - white screening
Components- SIG10 ULTRA electrocompetent cells
- pUC 19 transformation control DNA
- recovery medium for cloning
Legal InformationDH10B is a trademark of Life Technologies
DH5α is a trademark of Invitrogen Corp.Параметрыgrade for molecular biology form buffered aqueous solution cell transformation competent cell type: electrocompetent
transformation efficiency: 4 x 1010 cfu/µgshipped in dry ice storage temp. 70°C
Safety InformationRIDADR UN 3245 9 Узнать цену -
Sodium phosphate monobasic
Empirical Formula (Hill Notation): H2NaO4P Linear Formula: NaH2PO4 CAS Number: 7558-80-7 Molecular Weight: 119.98 EC Number: 231-449-2 MDL number: MFCD00003527 eCl@ss: 38070208 PubChem Substance ID: 24899546 NACRES: NA.52
Кат. номер S3139-1KG S3139-EW S3139-500G S3139-250G General descriptionSodium phosphate is a salt of sodium hydroxide and phosphoric acid. It is widely used in buffers for molecular biology applications.ApplicationSodium phosphate monobasic is widely used in buffers for molecular biology applications. It is an important ingredient in cholesterol assay reaction buffer and miRNA microarray fabrication.Packaging250, 500 g in poly bottle
1 kg in poly bottleПараметрыQuality Level 200 grade anhydrous, for molecular biology product line BioReagent assay 98% form solid pH 4.0-4.5 (25 °C, 50 g/L in water) solubility H2O: complete 100 mg/mL, clear, colorless anion traces chloride (Cl-): 40 ppm cation traces Fe: 30 ppm Featured Industry Diagnostic Assay Manufacturing foreign activity DNase, RNase and protease, none detected storage temp. room temp SMILES string [Na+].OP(O)([O-])=O InChI 1S/Na.H3O4P/c;1-5(2,3)4/h;(H3,1,2,3,4)/q+1;/p-1 InChI key AJPJDKMHJJGVTQ-UHFFFAOYSA-M
Safety InformationPersonal Protective Equipment Eyeshields, Gloves, type N95 (US) RIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
XLDNA SIG10 Electrocompetent cells CMC0012-20X25UL
NACRES: NA.85 General descriptionThe XLDNA SIG10 Electrocompetent cells are designed for transformation of large constructs and increase in DNA yields.
These cells are manufactured using a novel procedure that maximizes transformation efficiencies with very large inserts and are an excellent choice for large construct transformation
Genotype
F - pro A+B+ lacIqZM15::Tn10 (TetR)] /mcrA (mrr-hsdRMS-mcrBC) endA1 recA1 F80dlacZM15 lacX74 araD139 (ara, leu)7697 galU galK rpsL nupG - tonA (StrR)
Features and Benefits- Cells that enable the most challenging library construction and screening.
- Highest available transformation efficiencies for library construction and screening.
- Optimized for high yields of DNA
Components- XLDNA SIG10 electrocompetent cells
- pUC 19 transformation control DNA
- recovery medium for cloning
Параметрыgrade for molecular biology cell transformation competent cell type: electrocompetent
transformation efficiency: 1 x 1010 cfu/µgshipped in dry ice storage temp. 70°C
Safety InformationRIDADR UN 3245 9 Узнать цену -
XLDNA V2 Electrocompetent cells CMC0011-20X25UL
NACRES: NA.85 General descriptionThe XLDNA V2 Electrocompetent cells are designed for transformation of large constructs and increase in DNA yields.
Genotype
F- mcrA (mrr-hsdRMS-mcrBC) endA1 recA1 F80dlacZM15 lacX74 araD139 (ara,leu)7697 galUgalK rpsL nupG (attL araC-PBAD-trfA250 bla attR) -
Features and Benefits- Cells that enable the most challenging library construction and screening.
- Highest available transformation efficiencies for library construction and screening.
- Optimized for high yields of DNA
- Blue - white screening
Components- XLDNA V2 electrocompetent cells
- pKanR transformation control DNA
- recovery medium for cloning
Параметрыgrade for molecular biology cell transformation competent cell type: electrocompetent
transformation efficiency: 1 x 1010 cfu/µgshipped in dry ice storage temp. 70°C
Safety InformationRIDADR UN 3245 9 Узнать цену -
Yeast Promoter Vector Set PP2357-1KT
NACRES: NA.85 General descriptionMolecular cloning often benefits from optimizing the vector used for expression.
Use this plasmid pack to compare five different yeast promoters, each driving expression of your chosen gene inserted into the MCS. The pack includes strong, medium and weak constitutive promoters, and one inducible promoter. Each plasmid contains the URA3 selection gene. This should allow you easily to determine which promoter gives the desired level of expression of your gene of interest and to select for transformed yeast cells.This plasmid set has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI. The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site. Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Transcription Termination:<p>These plasmids contains three alternative transcription terminators for mammalian bacterial and bacteriophage (T7) expression. This means that only the promoter needs to be changed to alter the expression system you are using. We sell multiple promoters that can be used in each of these systems. The presence of each terminator does not reduce expression in the alternative systems.
Intellectual Property Status: According to our IP-friendly policy this plasmid is sold free of reach-through rights and can be used to make commercial products. However the plasmid itself (or derivatives) cannot be sold.
Sequence
Analysis NoteLegal InformationThese plasmids are sold free of reach-through rights and can be used to make commercial products. However the plasmids themselves (or derivatives) cannot be sold.Параметрыform buffered aqueous solution Origin of replication 2Micron, pUC (500 copies) Promoter Promoter name: TEF1
Promoter activity: constitutive
Promoter type: yeastbacteria selection kanamycin shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
YFP Vector Set PP2369-1KT
NACRES: NA.85 General descriptionMolecular cloning often benefits from optimizing the vector used for expression.
This pack allows you to determine the best configuration for the Yellow Fluorescence Protein (a non-Aequorea variant called Kringle YFP) reporter gene and to optimise expression for your specific needs. Each component plasmid contains the yellow fluorescent reporter regulated in a different way - in the MCS under the CMV promoter, under an IRES or regulated by your own chosen promoter, or regulated by independent promoters either immediately downstream of the MCS (sharing a polyA with the gene inserted into the MCS) or in a different part of the plasmid. This pack should enable you to compare different strategies for yellow fluorescence expression and chose the one that best suits your needs.This plasmid set has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI. The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site. Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Transcription Termination: These plasmids contains three alternative transcription terminators for mammalian bacterial and bacteriophage (T7) expression. This means that only the promoter needs to be changed to alter the expression system you are using. We sell multiple promoters that can be used in each of these systems. The presence of each terminator does not reduce expression in the alternative systems.
Analysis NoteOther NotesLooking for more vector options to move your experiments forward faster? Consider a custom cloning vector designed and built by Oxford Genetics™. Find out more at Oxford Genetics, - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications.Legal InformationThese plasmids are sold free of reach-through rights and can be used to make commercial products. However the plasmids themselves (or derivatives) cannot be sold.
Oxford Genetics is a trademark of Oxford Genetics LtdПараметрыform buffered aqueous solution Origin of replication pUC (500 copies) Promoter Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalianReporter gene location 2nd promoter, MCS bacteria selection ampicillin reporter gene YFP shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Zeocin Selection Vector Set PP2376-1KT
NACRES: NA.85 General descriptionMolecular cloning often benefits from optimizing the vector used for expression.
This pack allows you to determine the optimum expression configuration for zeocin resistance in your mammalian cell experimental system. The zeocin resistance gene is provided under regulatory control of four different promoters, and an IRES, and each plasmid can be further to modified to include your gene of interest if required.This plasmid set has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI. The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site. Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Transcription Termination: These plasmids contains three alternative transcription terminators for mammalian bacterial and bacteriophage (T7) expression. This means that only the promoter needs to be changed to alter the expression system you are using. We sell multiple promoters that can be used in each of these systems. The presence of each terminator does not reduce expression in the alternative systems.
Sequence
For Genebank sequence, FASTA sequence, Quick-reference Plasmid Map, and Full Plasmid Map, see the individual vector product page for links.
Analysis NoteOther NotesLooking for more vector options to move your experiments forward faster? Consider a custom cloning vector designed and built by Oxford Genetics™. Find out more at Oxford Genetics, - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications.Legal InformationThese plasmids are sold free of reach-through rights and can be used to make commercial products. However the plasmids themselves (or derivatives) cannot be sold.
Oxford Genetics is a trademark of Oxford Genetics Ltd
Zeocin is a registered trademark of Cayla SarlПараметрыform buffered aqueous solution Origin of replication pUC (500 copies) Promoter Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalianbacteria selection kanamycin mammalian cells selection Zeocin® shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
-Tri-Calcium phosphate
Empirical Formula (Hill Notation): Ca3O8P2 Linear Formula: Ca3(PO4)2 CAS Number: 7758-87-4 Molecular Weight: 310.18 EC Number: 231-840-8 MDL number: MFCD00015984 PubChem Substance ID: 329757609 NACRES: NA.52
Кат. номер 50553-10G-F 50553-VAR-F 50553-1G-F General descriptionα-tri-Calcium phosphate (α-TCP) can be made by heating the low-temperature polymorph β-TCP. It can also be made by thermal crystallization of amorphous precursors with the appropriate composition above the transformation temperature. α-TCP is biocompatible as β-TCP. It is highly soluble and hydrolyses quickly to calcium-deficient hydroxyapatite. This makes α-TCP a beneficial constituent for formulating self-setting osteotransductive bone cements and biodegradable bioceramics and composites for bone repairing.
α-tri-Calcium is a tasteless powder containing excess of calcium oxide. It is commonly used to introduce DNA and transfect eukaryotic cells for molecular biology experiments.ApplicationFor analysis of bone cement; as standard.
-tri-Calcium phosphate has been used to transfect 293T and 231 cells with the PiggyBac LSS-mOrange- aspartate-glutamate-valine-aspartate (DEVD)-mKate2 transposon vector and in reporter verification studies.
Suitable for- transient and stable transfection of wide range of cell types
- drug delivery systems
ПараметрыQuality Level 100 grade for molecular biology form powder application(s) transfection: suitable Featured Industry Cleaning Products
Cosmetics
Flavors and Fragrances
Food and Beverages
Personal Carestorage temp. room temp SMILES string [Ca++].[Ca++].[Ca++].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O InChI 1S/3Ca.2H3O4P/c;;;2*1-5(2,3)4/h;;;2*(H3,1,2,3,4)/q3*+2;;/p-6 InChI key QORWJWZARLRLPR-UHFFFAOYSA-H
Safety InformationPersonal Protective Equipment Eyeshields, Gloves, type N95 (US) RIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Calcium Phosphate Transfection Kit
EC Number: 233-140-8 NACRES: NA.85
Кат. номер CAPHOS-1KT CAPHOS-1KT-PW General descriptionCalcium phosphate transfection is a commonly used method for the introduction of DNA into eukaryotic cells. This technique has been used to obtain both transient and stable transfections in a wide variety of cell types.ApplicationCalcium Phosphate Transfection Kit has been used:- to enable transfection
- to transfect Hek293T cells
- to transfect H29D cells
Suitable for transient and stable transfection of DNA into cultured mammalian cells. The following cells have successfully been transfected using the calcium phosphate method:
BAEC
Bowels melanoma cells
CHO K1
COS-7
Fibroblasts (human embryonic, neo derm)
HEK293
Huh 7
IMR-90
LLC (Lewis Lung Carcinoma)
NIH3T3
PC-12
PCI-13
SH-Sy5Y
SK-Hep-1
T47DFeatures and Benefits- Suitable for transient and stable transfection
- Reproducible for a wide range of cell types
- Widely referenced
- Inexpensive
ComponentsThe Calcium Phosphate Transfection Kit contains:
5 ml 2.5M CaCl2 (C2052)
25 ml 2x HEPES Buffered Saline (H1012)
25 ml molecular biology grade water (W4502)PrincipleThe procedure is based on slow mixing of HEPES-buffered saline containing sodium phosphate with a CaCl2 solution containing the DNA. A DNA-calcium phosphate co-precipitate forms, which adheres to the cell surface and is taken up by the cell, presumably by endocytosis.Other NotesThe protocol was developed for transient transfection of CHO cells using pSV40-CAT plasmid as a reporter gene.ПараметрыQuality Level 200 grade for molecular biology form solution usage kit sufficient for 160 transfections (6 cm dishes), kit sufficient for 400 transfections (3.5 cm dishes), kit sufficient for 80 transfections (10 cm dishes) application(s) transfection: suitable shipped in dry ice storage temp. −20°C
Safety Informationpictograms GHS07 signalword Warning hcodes H319 pcodes P305 + P351 + P338 RIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
DAPI
Empirical Formula (Hill Notation): C16H15N5 · 2HCl CAS Number: 28718-90-3 Molecular Weight: 350.25 Beilstein/REAXYS Number: 4894417 EC Number: 249-186-7 MDL number: MFCD00012681 PubChem Substance ID: 24894305 NACRES: NA.52
Кат. номер D9542-50MG D9542-1MG D9542-10MG D9542-5MG D9542-50MG-PW D9542-1MG-PW D9542-10MG-PW D9542-5MG-PW General descriptionDAPI (4′,6-Diamidino-2-phenylindole dihydrochloride) is a cell permeable, fluorescent dye that binds to DNA.ApplicationSuitable for- DNA staining in agarose gels
- analysis of changes in DNA during apoptosis
- detection of mycoplasma
- photofootprinting of DNA
- immunofluorescent staining of cells
DAPI has been used:-- in rapid monitoring of microbial contamination
- in chromosomal banding technique
- in detection of apoptotic cells
- in fluorescence microscopy to track the DisA (DNA integritiy scanning protein) movement on Bacillus subtilis DNA
- to stain mature pollen grains(0.5 mg/ml)
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.Packaging1, 5, 10, 50 mg in glass bottleBiochem/physiol ActionsCell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.CautionProtect from light.ПараметрыQuality Level 300 grade for molecular biology assay 98% (HPLC and TLC) form powder application(s) transfection: suitable solubility H2O: 20 mg/mL (heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.), PBS: insoluble (extinction coefficient) 30 at 263 nm in H2O at 1 mM fluorescence ex 340 nm; em 488 nm (nur DAPI), ex 364 nm; em 454 nm (DAPI-DNA-Komplex) suitability suitable for fluorescence storage temp. 2-8°C SMILES string Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N InChI 1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H InChI key FPNZBYLXNYPRLR-UHFFFAOYSA-N
Safety Informationpictograms GHS07 signalword Warning hcodes H315 - H317 - H335 pcodes P261 - P264 - P271 - P272 - P280 - P302 + P352 Target Organs Respiratory system Personal Protective Equipment Eyeshields, Gloves, type N95 (US) RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
DAPI ready made solution
Empirical Formula (Hill Notation): C16H15N5 · 2HCl CAS Number: 28718-90-3 Molecular Weight: 350.25 Beilstein/REAXYS Number: 4894417 MDL number: MFCD00012681
Кат. номер MBD0015-10ML MBD0015-5ML MBD0015-50ML MBD0015-1ML General descriptionDAPI, 4′,6-diamidino-2-phenylindole, is a blue fluorescent nucleic acid stain that fluoresces brightly upon selectively binding to the minor groove of double stranded DNA. Its selectivity for DNA and high cell permeability allows efficient staining of nuclei with little background from the cytoplasm. The excitation and emission wavelengths of DAPI-DNA complex are 360 nm and 460 nm, respectively.ApplicationDAPI Readymade Solution is provided at 1mg/mL concentration. It is recommended to dilute DAPI readymade solution 1:1000 with a desired buffer for a 1µg/mL working concentration (typical working concentration used in most applications is 0.1µg/mL-10µg/mL). Store DAPI solution (1mg/mL) -20°C protected from light. It is recommended to aliquot DAPI readymade solution and to avoid freeze thaw cycles.
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.
For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS).
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.Biochem/physiol ActionsCell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.Features and Benefits- Ready-to-use liquid, cell permeable dye.
- Can be used with live cells and fixed cells.
- Effective for fixed-cell staining and quantitation of DNA content.
- Optimal Counterstain when using fluorescent antibodies during fluorescence microscopy or high-content screening (HCS) applications.
Physical formDAPI readymade solution is provided at 1mg/mL concentration in waterПараметрыform liquid storage condition protect from light concentration 1 mg/mL application(s) transfection: suitable fluorescence ex 340 nm; em 488 nm (nur DAPI), ex 364 nm; em 454 nm (DAPI-DNA-Komplex) shipped in dry ice storage temp. 20°C SMILES string Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N InChI 1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H InChI key FPNZBYLXNYPRLR-UHFFFAOYSA-N
Safety Informationpictograms GHS07 signalword Warning hcodes H317 pcodes P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 WGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
DAPI ready made solution with Antifade MBD0020-10ML
Empirical Formula (Hill Notation): C16H15N5 · 2HCl CAS Number: 28718-90-3 Molecular Weight: 350.25 Beilstein/REAXYS Number: 4894417 MDL number: MFCD00012681 General descriptionDAPI, 4′,6-diamidino-2-phenylindole, is a blue fluorescent nucleic acid stain that fluoresces brightly upon selectively binding to the minor groove of double stranded DNA. Its selectivity for DNA and high cell permeability allows efficient staining of nuclei with little background from the cytoplasm. The excitation and emission wavelengths of DAPI-DNA complex are 360 nm and 460 nm, respectively. Most of the fluorescent dyes fade (photobleach) when exposed to excitation light. The amount of photobleaching depends on the composition of the molecule and the time of light exposure. DAPI Ready Made Solution with Antifade is a formulation that suppress photobleaching and prolong fluorescent signals.ApplicationDAPI Ready Made Solution with Antifade is a glycerol based ready to use solution provided at 1μg/mL concentration in a dropper vial that can be applied directly to cells or tissue samples on microscope slides or coverslips. It is recommended to warm up at room temperature and mix by swirling the dropper vial before use. Store DAPI Ready Made Solution with antifade at -20 C protected from light.
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.Biochem/physiol ActionsCell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.Features and Benefits- Protects dyes from fading during imaging.
- Ready-to-use liquid.
- Easy to use Dropper vial application.
- Stained sample are stable for longer time period.
- Maintains fluorescence signal-little to no quenching.
Physical formLiquid - DAPI readymade with antifade solution is provided at 1μg/mL concentration in glycerol-PBS based solution.Параметрыform liquid storage condition protect from light application(s) transfection: suitable fluorescence ex 340 nm; em 488 nm (nur DAPI), ex 364 nm; em 454 nm (DAPI-DNA-Komplex) shipped in dry ice storage temp. 20°C SMILES string Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N InChI 1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H InChI key FPNZBYLXNYPRLR-UHFFFAOYSA-N
Safety Informationhcodes H412 pcodes P273 - P501 WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
DOTAP Liposomal Transfection Reagent 11202375001
Empirical Formula (Hill Notation): C43H83NO8S CAS Number: 144189-73-1 Molecular Weight: 774.19 MDL number: MFCD16660441 PubChem Substance ID: 329749323 General descriptionDOTAP Liposomal Transfection Reagent is a liposome formulation of the cationic lipid DOTAP. Unlike other liposomal reagents, it produces highly efficient transfections both in the presence and absence of serum. It can also be successfully used for in vivo applications. Mixing DOTAP with DNA results in spontaneously formed stable complexes. These complexes can be added directly to the cell culture medium. This method of DNA transfer is very gentle, avoiding the cytotoxic effects commonly encountered with lipofection or other transfection methods.ApplicationThe DOTAP (N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium methyl-sulfate) Liposomal Transfection Reagent is a liposome formulation of the cationic lipid DOTAP. DOTAP can be used for the highly efficient transfection of DNA including yeast artificial chromosomes (YACs) into eukaryotic cells for transient or stable gene expression, and is also suitable for the efficient transfer of other negatively charged molecules, such as RNA, oligonucleotides, nucleotides, ribonucleo-protein (RNP) complexes, and proteins into research samples of mammalian cells.
Cationic liposome-forming compound for transfection of DNA, RNA and other negatively charged molecules into eukaryotic cells. Mixing DOTAP with DNA results in spontaneously formed stable complexes that can be directly added to cell culture medium. These complexes fuse with the cell membrane and release DNA into the cytoplasm. The cells are transfected efficiently without cytotoxic effects. Use approximately 5-10 μg DOTAP per μg DNA and approximately 10-20 μg DOTAP per mL cell culture medium. The optimal working concentration and transfection time depends in part on the cell line used, and the type of material (RNA, DNA, protein) loaded.Features and Benefits- Easy to use: The lipid dispersion is simply mixed with the DNA solution, then applied directly to the cells
- Highly effective: 5 to 100-fold more effective than the calcium phosphate or DEAE-dextran method
- Gentle: No cytotoxic effects
- Flexible: Equally effective in the presence or absence of serum. Effective on a wide range of species (e.g., insect cells, mammalian cells) and a variety of different cell types. Can be used for either transient or stable transfection procedures.
- Aqueous dispersion (liposomes) in MBS (MES-buffered saline, pH 6.2) (bottled under argon), 1mg/ml, sterile-filtered.
Preparation NoteWorking concentration: Approximately 5 - 10µg DOTAP/µg DNA, and 10 - 20µg DOTAP/ml cell culture medium.
The optimal working concentration is dependent on several parameters, including the cell line being used, concentration and type of nucleic acid (DNA, RNA), and incubation time. It is important to optimize the transfection conditions for the individual cell type studied.
Cytotoxicity: Not cytotoxic up to a concentration of 100µg/ml (PBLs, HeLa cells).Other NotesFor life science research only. Not for use in diagnostic procedures.ПараметрыQuality Level 100 assay >99% (TLC) packaging pkg of 5 x 400 μL (10 U/μl) Торговая марка Roche application(s) transfection: suitable shipped in wet ice storage temp. 2-8°C SMILES string [H]C(C[N+](C)(C)C)(OC(CCCCCCC/C=C\CCCCCCCC)=O)COC(CCCCCCC/C=C\CCCCCCCC)=O.O=S([O-])(OC)=O InChI 1S/C42H80NO4.CH4O4S/c1-6-8-10-12-14-16-18-20-22-24-26-28-30-32-34-36-41(44)46-39-40(38-43(3,4)5)47-42(45)37-35-33-31-29-27-25-23-21-19-17-15-13-11-9-7-2;1-5-6(2,3)4/h20-23,40H,6-19,24-39H2,1-5H3;1H3,(H,2,3,4)/q+1;/p-1/b22-20-,23-21-; InChI key RSMRWWHFJMENJH-LQDDAWAPSA-M
Safety InformationRIDADR NONH for all modes of transport WGK Germany nwg Flash Point F does not flash Flash Point C does not flash Узнать цену -
Escort™ III Transfection Reagent
NACRES: NA.25
Кат. номер L3037-1ML L3037-1ML-PW L3037-SAMPLE-PW L3037-SAMPLE General descriptionEscort™ III is a unique formulation of a proprietary polycationic lipid and a neutral non-transfecting lipid. This liposome-forming compound is used for transfection of nucleic acids into primary cells.ApplicationSuitable for transient and stable transfection of nucleic acids into cultured eukaryotic cells. Use approximately 2-8 µl Escort™ III and 2 µg DNA per 6 cm cell culture plate. Protocol optimization provides very efficient transfection. The following cells have been successfully transfected using Escort™ III:
A549
C2C12 myotubes
Cardiomyocytes (rat)
COS-7
Fibroblasts (rat)
Germ cells (male rat)
Hepatocytes (rat and hamster)
HepG2
HeLa
Jurkat
Keratinocytes (human)
Myoblasts (mouse and quail)
Myocytes (mouse)
NIH3T3
PC-12
Retinal Neurons (rat)
Tracheobronchial cells (sheep)Features and Benefits- Suitable for stable and transient transfection
- Optimized for a wide variety of primary cells
- Low toxicity
- Compatible with both serum and serum-free transfection protocols
- Ideal for PC-12 cells
ComponentsEscort™ III formulation:
1 mg/mL total lipid in water
Note the identity of the lipids used in Escort™ III is confidential.CautionDo not freeze.PrincipleA stable complex is formed when Escort™ III is mixed with DNA in the absence of serum. The complexes are stable and can be directly added to the cell culture medium, where they fuse with the cell membrane, releasing the DNA into the cytoplasm. Note: complex formation is inhibited by serum, but once stable complexes have formed, the presence of serum is without consequence.Legal InformationEscort is a trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200 grade for molecular biology form liquid (aqueous solution) usage 1 mL sufficient for 250-1000 transfections concentration 1 mg/mL application(s) transfection: suitable storage temp. 2-8°C
Safety InformationPersonal Protective Equipment Eyeshields, Gloves RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Escort™ IV Transfection Reagent
NACRES: NA.25
Кат. номер L3287-1ML L3287-SAMPLE-PW L3287-1ML-PW L3287-SAMPLE General descriptionEscort™ IV is a unique formulation of a proprietary polycationic lipid and a neutral non-transfecting lipid. This liposome-forming compound is used for transfection of nucleic acids into a wide variety of eukaryotic cell types.ApplicationSuitable for transient and stable transfection of nucleic acids into cultured eukaryotic cells. Use approximately 4-16 µL Escort™ IV and 2 µg DNA per 6 cm cell culture plate. Protocol optimization provides very efficient transfection. For a list of cells that have been successfully transfected using Escort™ IV, see the Transfection Reagent Selection Guide.Features and Benefits- Suitable for stable and transient transfection
- Optimized for a wide variety of cell lines
- Low toxicity
- Compatible with both serum and serum-free transfection protocols
- Ideal for Sf9, Sf21 and S2 insect cells
ComponentsEscort™ IV formulation:
1 mg/mL total lipid in water
Note the identity of the lipids used in Escort™ IV is confidential.CautionDo not freeze.PrincipleA stable complex is formed when Escort™ IV is mixed with DNA in the absence of serum. The complexes are stable and can be directly added to the cell culture medium, where they fuse with the cell membrane, releasing the DNA into the cytoplasm. Note: complex formation is inhibited by serum, but once stable complexes have formed, the presence of serum is without consequence.Legal InformationEscort is a trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 100 grade for molecular biology form liquid (aqueous solution) usage 1 mL sufficient for 160-500 transfections concentration 1 mg/mL application(s) transfection: suitable storage temp. 2-8°C
Safety InformationPersonal Protective Equipment Eyeshields, Gloves RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Hygromycin B 10843555001
Empirical Formula (Hill Notation): C20H37N3O13 CAS Number: 31282-04-9 Molecular Weight: 527.52 MDL number: MFCD06795479 PubChem Substance ID: 329749191 General description- Formula: C20H37N3O13
- LD50: 6 mg/kg in mouse (i.v.);13 mg/kg in guinea pig (i.p.); 63 mg/kg in rat (i.p.)
- Molecular weight: Mr = 527.5
- Working concentration: 50 - 1,000 µg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 µg/ml. However, the optimal concentration must be experimentally determined, since it may vary depending on the type of cell used.
ApplicationHygromycin B is an aminoglycosidic antibiotic that inhibits protein synthesis in prokaryotes and eukaryotes. It is used to select and maintain the phenotype of eukaryotic cells that are stably transfected with the E. coli hygromycin-resistance gene (hyg or hph).Biochem/physiol ActionsMode of Action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation.
Antimicrobial Spectrum: Hygromycin B acts against bacteria, fungi and higher eukaryotic cells.QualityPurity: >80% (HPLC)Unit DefinitionUnit Assay: Unit/mg
We do not have this information.Physical formSolution, 50 mg/ml in PBS (phosphate-buffered saline), filtered through 0.2 μm pore size membranePreparation NoteWorking concentration: Recommended concentration for the selection of resistant cells is 50 to 1000 µg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 µg/ml. However, the optimal concentration must be determined experimentally, and may vary with the type of cell used.Storage and StabilityStore at 2 to 8 °C. (solution)Other NotesFor life science research only. Not for use in diagnostic procedures.
Solution, 50 mg/ml, in PBS (phosphate-buffered saline), filtered through 0.2 μm pore size membrane.Параметрыsterility non-sterile; 0.2 µm filtered assay 80% (HPLC) form buffered aqueous solution composition Hygromycin B, >80% HPLC packaging pkg of 20 mL (1 g) Торговая марка Roche concentration 50 mg/mL application(s) transfection: suitable shipped in wet ice SMILES string CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3O[C@]4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O InChI 1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1 InChI key GRRNUXAQVGOGFE-XKIAHZFYSA-N
Safety Informationpictograms GHS05,GHS06,GHS08 signalword Danger hcodes H301 - H312 - H317 - H318 - H330 - H334 pcodes P260 - P280 - P301 + P310 + P330 - P304 + P340 + P310 - P305 + P351 + P338 + P310 - P342 + P311 - P403 + P233 RIDADR UN 2810 6.1 / PGIII WGK Germany WGK 1 Flash Point F does not flash Flash Point C does not flash Узнать цену -
NeuroPorter™ Transfection Kit
NACRES: NA.25
Кат. номер NPT01-1KT NPT01-1KT-PW General descriptionNeuroporter™ Transfection Reagent is a unique formulation of a proprietary cationic lipid optimized for delivery of DNA into primary neurons, glial cells, and cultured neuronal cell lines with high efficiency and low toxicity. The Neuroporter™ Transfection Kit was designed for difficult-to-transfect primary neurons, addressing past problems such as poor cell viability, low transfection efficiency and neuro-degeneration.ApplicationSuitable for transient and stable transfection of nucleic acids into primary neurons and cultured neuronal cell lines. Use approximately 15-120 µl Neuroporter™ Transfection Reagent and 6-8 µg DNA (in provided unique DNA Dilution buffer when required) per 6 cm cell culture plate. The following cells have been successfully transfected using the Neuroporter™ Transfection Kit:- C6 glioma (human)
- Cortical neurons (rat primary)
- Dorsal Root Ganglion (DRG) cells (rat)
- NT2 neurons(human precursor cells)
- NT neurons (human differentiated cells)
- Subventricular Zone (SVZ) cells (mouse)
- White matter cells (mouse)
Features and Benefits- Optimized for primary neurons, glial cells, and cultured neural cell lines
- Very low toxicity with no neuro-degeneration or dendrite withdrawal
- Efficient DNA delivery primary neurons, glial cells, and cultured neural cell lines
- Fast and easy to use compared to other methods
- Compatible with both serum and serum-free transfection protocols
Components1 vial Neuroporter™ Transfection Reagent, dried lipid film (T2823)
1.5 mL Hydration Buffer H9036
7.5 mL DNA Diluent D1941CautionDo not freeze.PrincipleA stable, non-covalent complex is formed when the Neuroporter™ Transfection Reagent is mixed with DNA in the absence of serum. The complexes are stable and can be directly added to the cell culture medium, where they fuse with the cell membrane, releasing the DNA into the cytoplasm. Note: complex formation is inhibited by serum, but once stable complexes have formed, the presence of serum is without consequence.Legal InformationNeuroPorter is a trademark of Gene Therapy Systems, Inc.ПараметрыQuality Level 200 grade for molecular biology form dried film usage 1 kit sufficient for 75-200 transfections availability available only in USA, Canada and EU application(s) transfection: suitable storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
X-tremeGENE™ HP DNA Transfection Reagent
Кат. номер 6366236001 6366546001 6366244001 General descriptionX-tremeGENE HP DNA Transfection Reagent is a multi-component reagent that forms a complex with DNA , then transports the complex into animal or insect cells.Features and BenefitsX-tremeGENE HP DNA Transfection Reagent is a high performance reagent that transfects a broad range of eukaryotic cells, including insect cells and hard-to-transfect cell lines (e.g., Sf9, U-2 OS, MEF, MCF7, Hep G2, PC-3, HeLa, HT-29, HT-1080, K-562, RAW 264.7, Jurkat, PC-12, and HCT 116).- Benefit from an easy-to-use non-liposomal reagent that is free of animal-derived components, stable at room temperature, filtered through 0.2 µm pore size membrane, and active in serum-containing medium.
- Achieve new levels of transfection efficiency in primary cells and tumor cell lines that are not transfected well by other reagents.
- Generate physiologically relevant data using a reagent with low cytotoxic effects.
- Increase experimental throughput and enable target evaluation using a simple and consistent protocol.
QualityEach lot of X-tremeGENE HP DNA Transfection Reagent is carefully tested following established quality procedures to ensure that the product is consistently performing according to specifications. During quality testing, cells are transfected with a reporter gene vector DNA using X-tremeGENE HP DNA Transfection Reagent (ratio 3:1 l/g DNA). Reporter gene activity is monitored via chemiluminescent detection. Using a standard curve, the total amount of recombinant protein is determined per well in order to meet specification.Physical formSupplied in 80% ethanol and sterile-filtered through 0.2 μm pore size membrane. Does not contain any ingredients of human or animal origin.Number of Tests: Using the standard procedure, 1 ml of X-tremeGENE HP DNA Transfection Reagent can be used to perform up to 10,000 transfections in 96-well plates.Other NotesFor life science research only. Not for use in diagnostic procedures.Legal InformationX-tremeGENE is a trademark of RocheПараметрыQuality Level 100 grade for molecular biology form liquid (aqueous solution) usage 1 mL (suitable for 165 transfections) packaging pkg of 0.4 mL (06366244001), pkg of 1.0 mL (06366236001), pkg of 5 x 1 mL (06366546001) Торговая марка Roche application(s) transfection: suitable shipped in wet ice storage temp. 20°C
Safety InformationRIDADR UN1170 - class 3 - PG 2 - Ethanol, solution Узнать цену
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Все сотрудники имеют высшее образование, закончили ведущие химические вузы страны, такие как РХТУ им Менделеева.
У большинства компаний срок ожидания составляет 10-12 недель.
Оборудование хранится на сухом отапливаемом складе, где поддерживается ровная температура.
Работаем с PonyExpress и Деловыми линиями. Вы также можете выбрать свою транспортную компанию или забрать товар со склада в Москве.
В случае любых неполадок за свой счет выполним ремонт в сервисном центре или на заводе-изготовителе. Или бесплатно заменим прибор на новый.
Производим пуско-наладку оборудования, валидацию, обучение сотрудников. Если нужно, привлекаем инженеров с заводов- изготовителей.
