Cloning & Expression
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PSF-OXB20-SNAPFUSION - BACTERIAL SNAPFUSION CONSTIUTIVE PLASMID OGS605-5UG
NACRES: NA.85 General descriptionThis vector is a standard E. coli expression plasmid with the exception that it has been modified to allow the direct cloning of gene fragments that have been designed to fit with any of our peptide tag plasmid range. This can be useful if you have created a peptide fusion but you would also like to create a non-tagged gene variant. The multiple cloning site contains two BsgI and BseRI sites that produce overhangs that are compatible with gene designed using our SnapFusion primer designer (see the cloning tab for this product). The promoter in this plasmid is a strong constitutive bacterial promoter that does not require induction for activity.
Promoter Expression Level: This plasmid contains a very strong constitutive E. coli promoter that was derived from the RecA promoter by removing the LexA repressor site. It is part of our constitutive bacterial promoter range. This promoter shows the highest level of expression in the promoter range with OXB1 showing the lowest level. They require no inducing agent for expression.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 3866 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: OXB20
Promoter activity: constitutive
Promoter type: bacterialbacteria selection kanamycin reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-PA-CMVE - CMV ENHANCER PLASMID OGS42-5UG
General descriptionThis plasmid contains the major immediate early cytomegalovirus (CMV) enhancer region upstream of a small multiple cloning site (SalI to BstBI) that allows the insertion of a promoter of choice. The enhancer can be used to increase the strength of weaker promoters whilst often retaining any regulation that they already have such as tissue specificity or transcription factor activation in response to stimulus. This enhancer sequence has been inserted upstream of a synthetic poly-adenylation signal that reduces non- specific transcription of any gene that is inserted into the multiple cloning site.
Promoter Expression Level:ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 4355 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: enhancer
Promoter type: mammalianbacteria selection kanamycin reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-PA-CMVE-?GAL - CMV ENHANCER BETA GAL PLASMID OGS255-5UG
NACRES: NA.85 General descriptionThis plasmid contains the major immediate early cytomegalovirus (CMV) enhancer region upstream of the beta galactosidase (beta gal) reporter gene. The enhancer can be used to increase the strength of weaker promoters whilst often retaining any regulation that they already have such as tissue specificity or transcription factor activation in response to stimulus. This enhancer sequence has been inserted upstream of a synthetic poly-adenylation signal that reduces non-specific transcription of any gene that is inserted into the multiple cloning site.
Promoter Expression Level:ApplicationCloning in a gene: This plasmid contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusions proteins where the fusion gene may be positioned at the front or end of the MCS to allow gene fusion.
By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.
Multiple cloning site notes: In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.
BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 5886 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: enhancer
Promoter type: mammalianbacteria selection kanamycin reporter gene beta Gal shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-PA-CMVE-FLUC - CMV ENHANCER LUCIFERASE PLASMID OGS252-5UG
NACRES: NA.85 General descriptionThis plasmid contains the major immediate early cytomegalovirus (CMV) enhancer region upstream of the Photinus pyralis (FLuc) luciferase reporter. The enhancer can be used to increase the strength of weaker promoters whilst often retaining any regulation that they already have such as tissue specificity or transcription factor activation in response to stimulus. This enhancer sequence has been inserted upstream of a synthetic poly-adenylation signal that reduces non-specific transcription of any gene that is inserted into the multiple cloning site.
Promoter Expression Level:ApplicationCloning in a gene: This plasmid contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusions proteins where the fusion gene may be positioned at the front or end of the MCS to allow gene fusion.
By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.
Multiple cloning site notes: In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.
BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 4717 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: enhancer
Promoter type: mammalianbacteria selection kanamycin reporter gene firefly luciferase shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-PA-CMVE-RLUC - CMV ENHANCER RENILLA PLASMID OGS253-5UG
NACRES: NA.85 General descriptionThis plasmid contains the major immediate early cytomegalovirus (CMV) enhancer region upstream of the Renilla reniformis (RLuc) luciferase reporter. The enhancer can be used to increase the strength of weaker promoters whilst often retaining any regulation that they already have such as tissue specificity or transcription factor activation in response to stimulus. This enhancer sequence has been inserted upstream of a synthetic poly-adenylation signal that reduces non-specific transcription of any gene that is inserted into the multiple cloning site.
Promoter Expression Level:ApplicationCloning in a gene: This plasmid contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusions proteins where the fusion gene may be positioned at the front or end of the MCS to allow gene fusion.
By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.
Multiple cloning site notes: In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.
BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 5973 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: enhancer
Promoter type: mammalianbacteria selection kanamycin reporter gene Renilla luciferase shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-PA-CMVE-SEAP - CMV ENHANCER SEAP PLASMID OGS254-5UG
NACRES: NA.85 General descriptionThis plasmid contains the major immediate early cytomegalovirus (CMV) enhancer region upstream of the human secreted alkaline phosphatase (SEAP) reporter gene. The enhancer can be used to increase the strength of weaker promoters whilst often retaining any regulation that they already have such as tissue specificity or transcription factor activation in response to stimulus. This enhancer sequence has been inserted upstream of a synthetic poly-adenylation signal that reduces non-specific transcription of any gene that is inserted into the multiple cloning site.
Promoter Expression Level:ApplicationCloning in a gene: This plasmid contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusions proteins where the fusion gene may be positioned at the front or end of the MCS to allow gene fusion.
By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.
Multiple cloning site notes: In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.
BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 5259 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: enhancer
Promoter type: mammalianbacteria selection kanamycin reporter gene SEAP shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-PA-MINPROM - MINIMAL PROMOTER PLASMID OGS40-5UG
NACRES: NA.85 General descriptionThis plasmid contains a multiple cloning site in the promoter position designed to allow you to insert your own promoter to drive the expression of a gene of interest. The promoter multiple cloning site extends from the SalI restriction site to the BstBI restriction site. Downstream sites have other functions in our plasmid system for example adding n-terminal peptide tags.
Promoter Expression Level:ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 4041 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: HSV-TK
Promoter activity: minimal promoter
Promoter type: mammalianbacteria selection kanamycin reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-PA-MINPROM-FLUC - MINIMAL PROMOTER LUCIFERASE PLASMID OGS246-5UG
General descriptionThis plasmid contains a multiple cloning site that is upstream of a minimal promoter that is upstream of the Photinus pyralis (FLuc) luciferase. This plasmid can be used to insert specific DNA sequences that will endow the minimal promoter with specific biochemical properties such as tissue specificity or transcription factor specific activation.
Promoter Expression Level:ApplicationMultiple Cloning Site Notes: There is a start codon in the NcoI site in the MCS that can be removed by digestion with KpnI if required. The MCS for gene insertions extends from NotI to XbaI but there are built in Shine-Dalgarno and Kozak ribosomal entry site sequences aligned with the start codon in the NcoI site. The ClaI and NheI sites have other functions and allow the insertion of sequences from our other catalogue plasmids (such as IRES sequences and second promoters).
The BsgI and BseRI restriction sites in the plamsid cleave within the stop codon in the XbaI site in the MCS. This can allow retrospective fusion of coding sequences to our peptide tag range if your genes stop codon is positioned here.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 4533 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: HSV-TK
Promoter activity: minimal promoter
Promoter type: mammalianbacteria selection kanamycin reporter gene firefly luciferase shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-PA-MINPROM-RLUC - MINIMAL PROMOTER RENILLA LUC PLASMID OGS247-5UG
NACRES: NA.85 General descriptionThis plasmid contains a multiple cloning site that is upstream of a minimal promoter that is upstream of the Renilla reniformis (RLuc) luciferase. This plasmid can be used to insert specific DNA sequences that will endow the minimal promoter with specific biochemical properties such as tissue specificity or transcription factor specific activation.
Promoter Expression Level:ApplicationCloning in a gene: This plasmid contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusions proteins where the fusion gene may be positioned at the front or end of the MCS to allow gene fusion.
By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.
Multiple cloning site notes: In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.
BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 5659 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: HSV-TK
Promoter activity: minimal promoter
Promoter type: mammalianbacteria selection kanamycin reporter gene Renilla luciferase shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-PA-MINPROM-SEAP - MINIMAL PROMOTER SEAP PLASMID OGS248-5UG
NACRES: NA.85 General descriptionThis plasmid contains a multiple cloning site that is upstream of a minimal promoter that is upstream of the human secreted alkaline phosphatase (SEAP) reporter gene. This plasmid can be used to insert specific DNA sequences that will endow the minimal promoter with specific biochemical properties such as tissue specificity or transcription factor specific activation.
Promoter Expression Level:ApplicationMultiple Cloning Site Notes: There is a start codon in the NcoI site in the MCS that can be removed by digestion with KpnI if required. The MCS for gene insertions extends from NotI to XbaI but there are built in Shine-Dalgarno and Kozak ribosomal entry site sequences aligned with the start codon in the NcoI site. The ClaI and NheI sites have other functions and allow the insertion of sequences from our other catalogue plasmids (such as IRES sequences and second promoters).
The BsgI and BseRI restriction sites in the plamsid cleave within the stop codon in the XbaI site in the MCS. This can allow retrospective fusion of coding sequences to our peptide tag range if your genes stop codon is positioned here.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 4945 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: HSV-TK
Promoter activity: minimal promoter
Promoter type: mammalianbacteria selection kanamycin reporter gene SEAP shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-PA-PROMMCS - NO PROMOTER (MCS/POLYLINKER) PLASMID OGS186-5UG
NACRES: NA.85 General descriptionPSF-PA-PROMMCS-no promoter (MCS/POLYLINKER) plasmid does not contain a promoter upstream of the main multiple cloning site. Instead this plasmid vector contains a short multiple cloning site (SalI to BstBI) in the same position that all of our promoters are normally inserted (flanked by Bgl2 sites). This region also contains an artificial poly-adenylation sequence upstream of the multiple cloning site to reduce background read-through from interfering with any promoter that you insert.
Promoter Expression Level:ApplicationCloning in a gene: PSF-PA-PROMMCS – no promoter (MCS/POLYLINKER) plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 3864 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter
Promoter type: multiple cloning sitebacteria selection kanamycin reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-PA-PROMMCS-BETAGAL - NO PROMOTER (MCS) BETA GAL PLASMID OGS239-5UG
NACRES: NA.85 General descriptionThis plasmid contains a multiple cloning site in the promoter position designed to allow you to insert your own promoter to drive the expression of the reporter gene. In this plasmid it is upstream of the beta galactosidase (beta gal) reporter gene. The promoter multiple cloning site extends from the SalI restriction site to the BstBI restriction site. Downstream sites have other functions in our plasmid system for example adding n-terminal peptide tags.
Promoter Expression Level:ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 5395 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage bacteria selection kanamycin reporter gene none shipped in ambient storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-PA-PROMMCS-FLUC - NO PROMOTER (MCS) FIREFLY LUC PLASMID OGS236-5UG
General descriptionThis plasmid contains a multiple cloning site in the promoter position designed to allow you to insert your own promoter to drive the expression of the reporter gene. In this plasmid it is upstream of the Photinus pyralis (Fluc) luciferase reporter gene. The promoter multiple cloning site extends from the SalI restriction site to the BstBI restriction site. Downstream sites have other functions in our plasmid system for example adding n-terminal peptide tags.
Promoter Expression Level:ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 5927 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage bacteria selection kanamycin reporter gene none shipped in ambient storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-PA-PROMMCS-FRCFP - PROMOTERLESS MULTIPLE CLONING SITE CFP VECTOR OGS615-5UG
General descriptionFrosty CFP (Cyan Fluorescence Protein) expression plasmid with a multiple cloning site upstream to allow your preferred promoter to be inserted. Also contains a poly A site upstream to reduce background readthrough in mammalian systems
Promoter Expression Level:ApplicationCloning in a gene: This plasmid contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusions proteins where the fusion gene may be positioned at the front or end of the MCS to allow gene fusion.
By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.
Multiple cloning site notes: In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.
BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.
Analysis NoteПараметрыform buffered aqueous solution mol wt size 4613 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage bacteria selection kanamycin reporter gene none shipped in ambient storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-PA-PROMMCS-ILUMENA - PROMOTERLESS SECRETED LUCIFERASE PLASMID OGS616-5UG
General descriptionSecreted luciferase (iLumena) expression plasmid with a multiple cloning site upstream to allow your preferred promoter to be inserted. Also contains a poly A site upstream to reduce background readthrough in mammalian systems.
Promoter Expression Level:ApplicationCloning in a gene: This plasmid contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusions proteins where the fusion gene may be positioned at the front or end of the MCS to allow gene fusion.
By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.
Multiple cloning site notes: In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.
BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 4184 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage bacteria selection kanamycin reporter gene none shipped in ambient storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-PA-PROMMCS-KRYFP - PROMOTERLESS MULTIPLE CLONING SITE YFP PLASMID OGS614-5UG
General descriptionKringle YFP (Yellow Fluorescence Protein) expression vector with a multiple cloning site upstream to allow your preferred promoter to be inserted. Also contains a poly A site upstream to reduce background readthrough in mammalian systemsApplicationCloning in a gene: PSF-PA-PROMMCS-KRYFP - PROMOTERLESS MULTIPLE CLONING SITE YFP PLASMID contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusions proteins where the fusion gene may be positioned at the front or end of the MCS to allow gene fusion.
By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.
Multiple cloning site notes: In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.
BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.
Analysis NoteOther NotesOXGENEs technology platforms and expert solutions accelerate the discovery, development and manufacture of cell and gene therapies. We work with you to express your gene of interest at scale, with high levels of purity. In addition, our integrated bioinformatics and high-throughput gene editing technology creates a streamlined workflow for automated cell line production. learn more at www.oxgene.com ,Параметрыform buffered aqueous solution mol wt size 4613 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage bacteria selection kanamycin reporter gene none shipped in ambient storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-PA-PROMMCS-RLUC - NO PROMOTER (MCS) RENILLA PLASMID OGS237-5UG
NACRES: NA.85 General descriptionThis plasmid contains a multiple cloning site in the promoter position designed to allow you to insert your own promoter to drive the expression of the reporter gene. In this plasmid it is upstream of the Renilla reniformis (Rluc) luciferase reporter gene. The promoter multiple cloning site extends from the SalI restriction site to the BstBI restriction site. Downstream sites have other functions in our plasmid system for example adding n-terminal peptide tags.
Promoter Expression Level:ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 5482 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage bacteria selection kanamycin reporter gene none shipped in ambient storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-PA-PROMMCS-SEAP - NO PROMOTER (MCS) SEAP PLASMID OGS238-5UG
NACRES: NA.85 General descriptionThis plasmid contains a multiple cloning site in the promoter position designed to allow you to insert your own promoter to drive the expression of the reporter gene. In this plasmid it is upstream of the human secreted alkaline phosphatase (SEAP) reporter gene. The promoter multiple cloning site extends from the SalI restriction site to the BstBI restriction site. Downstream sites have other functions in our plasmid system for example adding n-terminal peptide tags.
Promoter Expression Level:ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 4768 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage bacteria selection kanamycin reporter gene none shipped in ambient storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-PGK - PHOSPHOGLYCERATE KINASE PROMOTER PLASMID OGS403-5UG
NACRES: NA.85 General descriptionPSF-PGK - PHOSPHOGLYCERATE KINASE PROMOTER PLASMID contains the PGK (murine) promoter upstream of the multiple cloning site (MCS) for expression in mammalian cells. Transcription termination is mediated by the SV40 poly-adenylation signals downstream of the MCS.ApplicationCloning in a gene: Expression vector has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 4172 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: PGK
Promoter activity: constitutive
Promoter type: mammalianbacteria selection kanamycin reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-PGK-EMCV-PURO - PGK PROMOTER EMCV IRES PUROMYCIN VECTOR OGS612-5UG
General descriptionThis plasmid vector contains the phosphoglycerate kinase (PGK) promoter upstream of the MCS and EMCV IRES regulating expression of puromycin resistance
Promoter Expression Level: The murine phosphoglycerate kinase (PGK) promoter driving expression allows for long term stable expression in vitro and in vivo in most types. The IRES that is used to drive puromycin resistance downstream of the PGK promoter produces significantly weaker expression compared to the upstream gene (typically 15-20 fold lower than the upstream gene).ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 4172 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: PGK
Promoter activity: constitutive
Promoter type: mammalianbacteria selection kanamycin mammalian cells selection puromycin reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-PROMMCS - NO-PROMOTER MCS PLASMID OGS402-5UG
NACRES: NA.85 General descriptionThis vector does not contain a promoter upstream of the main multiple cloning site. Instead this vector contains a short multiple cloning site (SalI to BstBI) in the same position that all of our promoters are normally inserted (flanked by Bgl2 sites). This plasmid is therefore ideal for inserting new promoters.
Promoter Expression Level:ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 3806 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage bacteria selection kanamycin reporter gene none shipped in ambient storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-PROMMCS-BETAGAL - NO-PROMOTER MCS BETA GAL PLASMID OGS372-5UG
NACRES: NA.85 General descriptionThis plasmid contains a multiple cloning site in the promoter position designed to allow you to insert your own promoter to drive the expression of the reporter gene. In this plasmid it is upstream of the beta galactosidase (beta gal) reporter gene. The promoter multiple cloning site extends from the SalI restriction site to the BstBI restriction site. Downstream sites have other functions in our plasmid system for example adding n-terminal peptide tags.
Promoter Expression Level:ApplicationCloning in a gene: This plasmid contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusions proteins where the fusion gene may be positioned at the front or end of the MCS to allow gene fusion.
By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.
Multiple cloning site notes: In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.
BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 6918 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage bacteria selection kanamycin reporter gene none shipped in ambient storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-PROMMCS-FLUC - NO-PROMOTER MCS LUCIFERASE PLASMID OGS369-5UG
NACRES: NA.85 General descriptionThis plasmid contains a multiple cloning site in the promoter position designed to allow you to insert your own promoter to drive the expression of the reporter gene. In this plasmid it is upstream of the Photinus pyralis (Fluc) luciferase reporter gene. The promoter multiple cloning site extends from the SalI restriction site to the BstBI restriction site. Downstream sites have other functions in our plasmid system for example adding n-terminal peptide tags.
Promoter Expression Level:ApplicationCloning in a gene: This plasmid contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusions proteins where the fusion gene may be positioned at the front or end of the MCS to allow gene fusion.
By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.
Multiple cloning site notes: In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.
BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 5424 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage bacteria selection kanamycin reporter gene none shipped in ambient storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-PROMMCS-RLUC - NO-PROMOTER MCS LUCIFERASE PLASMID OGS370-5UG
NACRES: NA.85 General descriptionThis plasmid contains a multiple cloning site (MCS) in the promoter position designed to allow you to insert your own promoter to drive the expression of the reporter gene. In this plasmid the MCS is upstream of the Renilla reniformis (Rluc) luciferase reporter gene. The promoter multiple cloning site extends from the SalI restriction site to the BstBI restriction site. Downstream sites have other functions in our plasmid system for example adding n-terminal peptide tags.
Promoter Expression Level:ApplicationCloning in a gene: This plasmid contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is in this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusion proteins where the fusion gene may be positioned at the front or end of the MCS to allow gene fusion.
By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.
Multiple cloning site notes: In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.
BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 4710 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage bacteria selection kanamycin reporter gene none shipped in ambient storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-PROMMCS-SEAP - NO-PROMOTER MCS SEAP PLASMID OGS371-5UG
NACRES: NA.85 General descriptionThis plasmid contains a multiple cloning site in the promoter position designed to allow you to insert your own promoter to drive the expression of the reporter gene. In this plasmid it is upstream of the human secreted alkaline phosphatase (SEAP) reporter gene. The promoter multiple cloning site extends from the SalI restriction site to the BstBI restriction site. Downstream sites have other functions in our plasmid system for example adding n-terminal peptide tags.
Promoter Expression Level:ApplicationCloning in a gene: This plasmid contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusions proteins where the fusion gene may be positioned at the front or end of the MCS to allow gene fusion.
By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.
Multiple cloning site notes: In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.
BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 5337 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage bacteria selection kanamycin reporter gene none shipped in ambient storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-RENILLA-PHOTINUS - BI-DIRECTIONAL FIREFLY RENILLA LUCIFERASE PLASMID OGS595-5UG
NACRES: NA.85 General descriptionThis plasmid has been designed so that sequences can inserted between two reporter genes (renilla and firefly luciferase) to allow the relative transcription level in both directions to be determined by measuring the reporter luminescence. Dual luciferase reporter assay kits are available from other providers that are compatible with this plasmid. The plasmid only contains a Bgl2 site between the two genes to allow insertion.
Promoter Expression Level:ApplicationThis plasmid contains two luciferase reporter genes (Firefly and Renilla) in opposing directions with a Bgl2 restriction site in between the two genes to allow promoters to be inserted. This allows the transcriptional level of DNA sequences and promoters to be determined in both directions by inserting the sequences into the Bgl2 restriction site. If the sequence you would like to insert contains a Bgl2 site fragments with BamHI also have the same overhangs and can be ligated into this plasmid cut with Bgl2.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 6170 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage bacteria selection kanamycin reporter gene Renilla luciferase shipped in ambient storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-RSV - RSV PROMOTER PLASMID OGS146-5UG
NACRES: NA.85 General descriptionThis plasmid vector for molecular cloning contains the RSV promoter upstream of the multiple cloning site (MCS) for expression in mammalian cells. This promoter is one of the weaker constitutive promoters that we sell. Transcription termination is mediated by the SV40 poly-adenylation signals downstream of the MCS.
Promoter Expression Level:ApplicationCloning in a gene: PSF-RSV - RSV promoter plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 4122 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: RSV
Promoter activity: constitutive
Promoter type: mammalianbacteria selection kanamycin reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-SP6 - SP6 PROMOTER PLASMID OGS38-5UG
NACRES: NA.85 General descriptionThis vector allows transcription of un-polyadenylated and un-capped RNA using the SP6 bacteriophage polymerase. Some in vitro transcription kits will enable the production poly-adenylated and capped RNA using this vector; however this will depend on the kit used. SnapFast vectors do not contain an SP6 terminator and so to prevent the polymerase from circling the plasmid it must be cleaved at a restriction site that is 3 prime to the end of your gene to allow run-off of the polymerase. If you are expressing your gene in a vaccinia virus-free mammalian SP6 system it will require an IRES upstream of your gene because of the absence of a 5 prime cap on the RNA produced.
Promoter Expression Level:ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 3862 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage bacteria selection kanamycin reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-STE5-URA3 - WEAK PROMOTER YEAST PLASMID OGS535-5UG
NACRES: NA.85 General descriptionThis plasmid is designed for low level expression of proteins in Saccharomyces cerevisiae using the STE5 promoter. This is the weakest of the promoters that we sell for expression in yeast. The plasmid also contains a gene that is an essential component of the uracil synthesis pathway. This allows for the selection of yeast cells that are defective in this gene in media that does not contain uracil.
Promoter Expression Level: This plasmid contains the yeast scaffold protein STE5 promoter. It is the weakest promoter that we provide for expression in Saccharomyces cerevisiae.ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 6519 bp Origin of replication 2Micron, pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: TEF1
Promoter activity: constitutive
Promoter type: yeastbacteria selection kanamycin reporter gene none shipped in ambient storage temp. 20°C yeast selection uracil
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-SV40 - SV40 PROMOTER PLASMID OGS47-5UG
NACRES: NA.85 General descriptionPSF-SV40 - SV40 PROMOTER PLASMID contains the Simian Virus 40 promoter upstream of the multiple cloning site (MCS) for expression in mammalian cells. Transcription termination is mediated by the SV40 poly-adenylation signals downstream of the MCS.ApplicationCloning in a gene: PSF-SV40 - SV40 PROMOTER PLASMID has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.Analysis NoteПараметрыform buffered aqueous solution mol wt size 3867 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: SV40
Promoter activity: constitutive
Promoter type: mammalianbacteria selection kanamycin reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-SYN1 - SYNAPSIN 1 NEURON SPECIFIC PROMOTER PLASMID OGS503-5UG
NACRES: NA.85 General descriptionThis plasmid vector contains the human Synapsin 1 neuron specific promoter and be used in mammalian cells to drive neuron specific expression of transgenes.
Promoter Expression Level: This plasmid contains the human Synapsin 1 promoter which is specifically expressed in neurons and can be used for tissue specific expression of transgenes.ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 4068 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalianbacteria selection kanamycin reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-T3 - T3 PROMOTER PLASMID OGS39-5UG
NACRES: NA.85 General descriptionThis vector allows transcription of un-polyadenylated and un-capped RNA using the T3 bacteriophage polymerase. Some in vitro transcription kits will enable the production poly-adenylated and capped RNA using this vector; however this will depend on the kit used. SnapFast vectors do not contain an T3 terminator and so to prevent the polymerase from circling the plasmid it must be cleaved at a restriction site that is 3 prime to the end of your gene to allow run-off of the polymerase. If you are expressing your gene in a vaccinia virus-free mammalian T3 system it will require an IRES upstream of your gene because of the absence of a 5 prime cap on the RNA produced.
Promoter Expression Level:ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 3862 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage bacteria selection kanamycin reporter gene none shipped in ambient storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-T7 - T7 PROMOTER PLASMID OGS37-5UG
NACRES: NA.85 General descriptionPSF-T7 - T7 promoter plasmid allows transcription of RNA using the T7 bacteriophage polymerase. If you are using an in vitro transcription kit the RNA produced from this vector will be uncapped and not poly-adenylated making it unsuitable for expression in mammalian cells however some kits are available that will add these mRNA features. The PSF-T7 - T7 promoter can also be used for inducible gene expression in cell lines that contain the T7 polymerase.
Almost all SnapFast vectors contain a T7 terminator downstream of the multiple cloning site that will enable transcription termination. If required the vector can also be digested at a restriction site that is 3 prime to the end of your gene to allow run-off of the polymerase rather than exploiting the hairpin T7 terminator. If you are expressing your gene in a Vaccinia virus-free mammalian T7 system it will require an IRES upstream of your gene because of the absence of a 5 prime cap on the RNA produced.
Promoter Expression Level:ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 3862 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: T7
Promoter activity: inducible
Promoter type: phagebacteria selection kanamycin reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-T7-EMCV - T7 IRES EXPRESSION PLASMID OGS185-5UG
NACRES: NA.85 General descriptionPSF-T7-EMCV - T7 IRES EXPRESSION PLASMID allows transcription of un-polyadenylated and un-capped RNA using the T7 bacteriophage polymerase. This plasmid also contains the internal ribosome entry site (IRES) from Encephalomyocarditis virus (ECMV) between the promoter and the multiple cloning site (MCS). It is designed to be used to express genes using eukaryotic T7 expression systems such as those used for the recovery of a variety of viruses. This vector is not designed for the expression of two proteins from the same mRNA. For this purpose we have designed pSF-CMV-MCS-IRES-PciI where the PciI site contains a start codon in the correct position for the IRES to express efficiently. In this vector there is an NcoI site (which contains an ATG start codon) in the correct position to enable efficient expression of a gene in the MCS.ApplicationThe start codon that is within the NcoI restriction site is positioned to allow efficient expression from the ECMV IRES. The main MCS for gene insertions in this molecular cloning vector therefore extends from NcoI to XbaI. Downstream sites have alternate functions and are used to insert other DNA sequences that we sell however they can be used to insert a gene if these functions are not required.
The ClaI to NheI sites have other functions such as adding peptide tags second promoters or other IRES elements. The BsgI and BseRI restriction sites cleave within the stop codon in the XbaI site and allow the retrospective fusion of coding sequences. These sites are normally only used on genes that we sell in the main multiple cloning site.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 4378 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: T7
Promoter activity: inducible
Promoter type: phagebacteria selection kanamycin reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-T7-NH2-OMPA SP-NCOI - BACTERIAL SECRETION PLASMID OGS165-5UG
NACRES: NA.85 General descriptionThis vector allows transcription of un-polyadenylated and un-capped RNA using the T7 bacteriophage polymerase. This vector also contains the Escherichia coli (E. coli) outer membrane protein A (OmpA) secretory signal peptide. When positioned at the N-terminus of a protein it should direct the protein to be secreted out of the bacterial cytosol and into the periplasmic space. The OmpA secretory tag is 21 amino acids in length. The signal peptide protein sequence is MKKTAIAIAVALAGFATVAQA. Cleavage occurs after the final Alanine residue. The peptide tag will be cleaved the mature protein during export. In this vector the signal peptide coding sequence has been positioned adjacent to the NcoI restriction site.
This technique can be used to increase di-sulphide bond formation and also reduces proteolytic degradation. This method also reduces contamination from endogenous proteins because very few E. coli proteins are secreted. A sub fraction of the protein secreted into the periplasmic space can also extracted from the growth media. It is still unclear why this occurs but it believed to reflect increased permeability of the outer membrane particularly during extended culture periods.
Secretion of proteins in E. coli can sometimes be difficult. Potential issues include protein toxicity within the periplasm variable secretion efficiency formation of inclusion bodies within the cytoplasm when using strong promoters incorrect dis-sulphide formation. Background to bacterial secretion systems including possible solutions to these problems can be found in Choi et al 2004 Applied Microbiology and Biotechnology 64 (625-635).
all our vectors (except those in the terminators category on our website) will contain a T7 terminator downstream of the multiple cloning site. This will enable transcription termination.
Promoter Expression Level:ApplicationCloning in a gene: This vector has been designed to allow the addition of a peptide tag to the end of a protein of interest using standard cloning techniques.Multiple Cloning Site Notes:
There is a start codon in the NcoI site can be removed by digestion with KpnI if required. The MCS for gene insertions extends from NotI to XbaI however the tag resides between the NotI and HindIII sites. There are Shine-Dalgarno sequences and KOZAK sequences aligned with the start codon of the peptide tag.
The ClaI to NheI sites have other functions such as adding C-terminal peptide tags second promoters or IRES expression components. The BsgI and BseRI restriction sites cleave within the stop codon in the XbaI site and allow the retrospective fusion of C-terminal peptide tags sequences if the stop codon is placed in this position.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 3900 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Peptide tag location N-terminal Promoter Promoter name: T7
Promoter activity: inducible
Promoter type: phageSecretion signal OmpA bacteria selection kanamycin reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-T7-T7 TERM - T7 SINGLE TERMINATOR PLASMID OGS177-5UG
NACRES: NA.85 General descriptionPSF-T7-T7 TERM - T7 SINGLE TERMINATOR PLASMID allows transcription of RNA using the T7 bacteriophage polymerase. If you using an in vitro transcription kit the RNA produced from this vector will be uncapped and poly-adenylated making it unsuitable for mammalian cells however some kits are available that will add these features. This plasmid can also be used for inducible gene expression in bacterial cell lines that express the T7 polymerase.
This expression vector contains the T7 terminator only whereas most of our vectors contain mammalian T7 and bacterial termination signals together. This plasmid is normally only used if the presence of the mammalian and bacterial terminators is undesirable.
This molecular cloning vector can also be digested at a restriction site that is 3 prime to the end of your gene to allow run-off of the polymerase rather than exploiting the hairpin T7 terminator. If you are expressing your gene in a Vaccinia virus-free mammalian T7 system it will require an IRES upstream of your gene because of the absence of a 5 prime cap on the RNA produced please see OG85: pSF-T7-EMCV if this is required.ApplicationCloning in a gene: PSF-T7-T7 TERM - T7 SINGLE TERMINATOR PLASMID has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 3448 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: T7
Promoter type: phagebacteria selection kanamycin reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-T7/LACO - T7 LACO INDUCIBLE BACTERIAL PLASMID OGS500-5UG
NACRES: NA.85 General descriptionPSF-T7/LACO - T7 LACO inducible bacterial plasmid contains T7 promoter that can be regulated using the Lac repressor. The T7 LACO plasmid allows greater control over expression in comparison to normal T7 promoter plasmids. In order to use this inducible bacterial plasmid you will require a T7 polymerase expressing E. coli cell line that also expresses the LacI repressor. A common expression cell line that can be used for this purpose is the BL21 (DE3) strain.
Promoter Expression Level: This plasmid contains the T7 promoter followed by the lac operon operator sequence. This promoter is useful for inducible expression in bacterial cells that express the T7 polymerase.ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 3883 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: T7 Lac
Promoter activity: inducible
Promoter type: phagebacteria selection kanamycin reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
pSF-T7LacO-NH2-FLAG OGS636-5UG
NACRES: NA.85 General descriptionThe pSF-T7LacO-NH2-FLAG expression vector is a 5.5 kb E. coli expression vector used for cloning and cytoplasmic expression of a properly inserted open reading frame as an N-terminal Met-FLAG fusion protein. The FLAG epitope is a small- hydrophilic- 8 amino acid-tag (DYKDDDDK) that enables sensitive detection and high quality protein purification using anti-FLAG products. The N-terminal Met-FLAG fusion protein may be detected using monoclonal anti-FLAG M1 (catalogue number F3040) or M2 (F3165) antibodies. The fusion protein may be purified using anti-FLAG affinity gel (A2220). In this vector, transcription of the fusion protein is driven by the strong phage T7 promoter. This requires the use of E. coli cells containing a source of the T7 polymerase, such as BL21(DE3)T1R cells (catalogue number B2935).ApplicationCloning in a gene: This plasmid contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusions proteins where the fusion gene may be positioned at the front or end of the MCS to allow gene fusion.By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.Multiple cloning site notes:In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.
Sequence
Analysis NoteOther NotesLooking for more vector options to move your experiments forward faster? Consider a custom cloning vector designed and built by Oxford Genetics™. Find out more at Oxford Genetics, - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications.Legal InformationOxford Genetics is a trademark of Oxford Genetics Ltd
FLAG is a registered trademark of Sigma-Aldrich Co. LLC
Oxford Genetics is a trademark of Oxford Genetics LtdПараметрыrecombinant expressed in E. coli form buffered aqueous solution mol wt size 5496 bp conjugate FLAG® tagged Origin of replication pUC Peptide cleavage EKT Peptide tag location N-terminal Promoter Promoter name: T7 Lac
Promoter activity: inducible
Promoter type: bacterialbacteria selection ampicillin shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-TAC - PTAC INDUCIBLE BACTERIAL PLASMID OGS501-5UG
NACRES: NA.85 General descriptionThis is a bacterial expression plasmid containing pTAC promoter that can be regulated using the Lac repressor. The pTac promoter was created by fusing the promoter for the Lac operon with the promoter for the Tryptophan operon. This provides greater control and expression in comparison to standard Lac promoter plasmids. In order to use this plasmid you will need an E. coli cell line that expresses the LacI repressor this is a common feature of most cloning and protein expression E. coli strains.
Promoter Expression Level: This plasmid contains the IPTG inducible promoter that was created by the fusion of the Lac promoter and the Tryptophan operon promoter. It allows inducible expression in E. coli using IPTG as the inducing agent.ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 3864 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: Tac
Promoter activity: inducible
Promoter type: bacterialbacteria selection kanamycin reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
pSF-TAC-COOH-FLAG OGS634-5UG
NACRES: NA.85 General descriptionThe pSF-TAC-COOH-FLAG expression vector is a 5.5 kb E. coli expression vector used for cloning and cytoplasmic expression of a properly inserted open reading frame as a C-terminal FLAG fusion protein. The FLAG epitope is a small- hydrophilic- 8 amino acid-tag (DYKDDDDK) that enables sensitive detection and high quality protein purification using anti-FLAG products. The C-terminal FLAG fusion protein may be detected using monoclonal anti-FLAG M2 antibody (catalogue number F3165).The fusion protein may be purified using anti-FLAG affinity gel (A2220). In this vector, transcription is driven by the IPTG inducible TAC promoter.ApplicationCloning in a gene: This plasmid contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusions proteins where the fusion gene may be positioned at the front or end of the MCS to allow gene fusion.By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.Multiple cloning site notes:In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.
Sequence
Analysis NoteOther NotesLooking for more vector options to move your experiments forward faster? Consider a custom cloning vector designed and built by Oxford Genetics™. Find out more at Oxford Genetics, - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications.Legal InformationOxford Genetics is a trademark of Oxford Genetics Ltd
FLAG is a registered trademark of Sigma-Aldrich Co. LLC
Oxford Genetics is a trademark of Oxford Genetics LtdПараметрыrecombinant expressed in E. coli form buffered aqueous solution mol wt size 5458 bp conjugate FLAG® tagged Origin of replication pUC Peptide cleavage EKT Peptide tag location C-terminal Promoter Promoter name: Tac
Promoter activity: inducible
Promoter type: bacterialbacteria selection ampicillin shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
pSF-TAC-NH2-FLAG OGS632-5UG
NACRES: NA.85 General descriptionThe pSF-TAC-NH2-FLAG expression vector is a 5.5 kb E. coli expression vector used for cloning and cytoplasmic expression of a properly inserted open reading frame as an N-terminal Met-FLAG fusion protein. The FLAG epitope is a small- hydrophilic- 8 amino acid-tag (DYKDDDDK) that enables sensitive detection and high quality protein purification using anti-FLAG products. The N-terminal Met-FLAG fusion protein may be detected using monoclonal anti-FLAG M1 (catalogue number F3040) or M2 (F3165) antibodies. The fusion protein may be purified using anti-FLAG affinity gel (A2220). In this vector, transcription is driven by the IPTG inducible TAC promoter.
This product is an alternative to our original pFLAG vector, product number E8083.ApplicationCloning in a gene: This plasmid contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusions proteins where the fusion gene may be positioned at the front or end of the MCS to allow gene fusion.By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.Multiple cloning site notes:In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.
Sequence
Analysis NoteOther NotesLooking for more vector options to move your experiments forward faster? Consider a custom cloning vector designed and built by Oxford Genetics™. Find out more at Oxford Genetics, - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications.Legal InformationOxford Genetics is a trademark of Oxford Genetics Ltd
FLAG is a registered trademark of Sigma-Aldrich Co. LLC
Oxford Genetics is a trademark of Oxford Genetics LtdПараметрыrecombinant expressed in E. coli form buffered aqueous solution mol wt size 5477 bp conjugate FLAG® tagged Origin of replication pUC Peptide cleavage EKT Peptide tag location N-terminal Promoter Promoter name: Tac
Promoter activity: inducible
Promoter type: bacterialbacteria selection ampicillin shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
pSF-TAC-NH2-OmpA-COOH-FLAG OGS635-5UG
NACRES: NA.85 General descriptionThe pSF-TAC-NH2-OmpA-COOH-FLAG expression vector is a 5.5 kb E. coli expression vector used for cloning and periplasmic expression of a properly inserted open reading frame as a C-terminal FLAG fusion protein. The FLAG epitope is a small- hydrophilic- 8 amino acid-tag (DYKDDDDK) that enables sensitive detection and high quality protein purification using anti-FLAG products. The C-terminal FLAG fusion protein may be detected using monoclonal anti-FLAG M2 antibody (catalogue number F3165).The fusion protein may be purified using anti-FLAG affinity gel (A2220). In this vector, transcription is driven by the IPTG inducible TAC promoter.ApplicationCloning in a gene: This plasmid contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusions proteins where the fusion gene may be positioned at the front or end of the MCS to allow gene fusion.By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.Multiple cloning site notes:In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.
Sequence
Analysis NoteOther NotesLooking for more vector options to move your experiments forward faster? Consider a custom cloning vector designed and built by Oxford Genetics™. Find out more at Oxford Genetics, - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications.Legal InformationOxford Genetics is a trademark of Oxford Genetics Ltd
FLAG is a registered trademark of Sigma-Aldrich Co. LLC
Oxford Genetics is a trademark of Oxford Genetics LtdПараметрыrecombinant expressed in E. coli form buffered aqueous solution mol wt size 5502 bp conjugate FLAG® tagged Origin of replication pUC Peptide cleavage EKT Peptide tag location C-terminal Promoter Promoter name: Tac
Promoter activity: inducible
Promoter type: bacterialSecretion signal OmpA bacteria selection ampicillin shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
pSF-TAC-NH2-OmpA-FLAG OGS633-5UG
NACRES: NA.85 General descriptionThe pSF-TAC-NH2-OmpA-FLAG expression vector is a 5.5 kb E. coli expression vector used for cloning and periplasmic expression of a properly inserted open reading frame as an N-terminal Met-FLAG fusion protein. The FLAG epitope is a small- hydrophilic- 8 amino acid-tag (DYKDDDDK) that enables sensitive detection and high quality protein purification using anti-FLAG products. The N-terminal Met-FLAG fusion protein may be detected using monoclonal anti-FLAG M1 (catalogue number F3040) or M2 (F3165) antibodies. The fusion protein may be purified using anti-FLAG affinity gel (A2220). In this vector, transcription is driven by the IPTG inducible TAC promoter.ApplicationCloning in a gene: This plasmid contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusions proteins where the fusion gene may be positioned at the front or end of the MCS to allow gene fusion.By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.Multiple cloning site notes:In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.
Sequence
Analysis NoteOther NotesLooking for more vector options to move your experiments forward faster? Consider a custom cloning vector designed and built by Oxford Genetics™. Find out more at Oxford Genetics, - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications.Legal InformationOxford Genetics is a trademark of Oxford Genetics Ltd
FLAG is a registered trademark of Sigma-Aldrich Co. LLC
Oxford Genetics is a trademark of Oxford Genetics LtdПараметрыrecombinant expressed in E. coli form buffered aqueous solution mol wt size 5534 bp conjugate FLAG® tagged Origin of replication pUC Peptide cleavage EKT Peptide tag location N-terminal Promoter Promoter name: Tac
Promoter activity: inducible
Promoter type: bacterialSecretion signal OmpA bacteria selection ampicillin shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-TEF1-COOH-10HIS - C-TERMINAL 10HIS TAG YEAST PLASMID OGS3455-5UG
NACRES: NA.85 General descriptionThis plasmid is designed to express tagged proteins in yeast cells (Saccharomyces cerevisiae). The plasmid contains an auxotrophic Uracil selection expression cassette (URA3) that allows for the positive selection of yeast that are deficient in the URA3 gene (YEL021W). This is typically achieved by growing the yeast in minimal media that is reconstituted with the essential amino acids and nucleotides but excluding Uracil.
About the Peptide Tag:This plasmid contains a c-terminal Deca-Histidine (10His) affinity tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: HHHHHHHHHH.
About the Cleavage Tag:This plasmid does not contain a protease cleavage site.
Promoter Expression Level: This plasmid contains the Yeast Elongation Factor Alpha promoter (TEF1). This is the strongest of the yeast promoters that we sell. It is a constitutive promoter and requires no induction. If you are interested in weaker promoters levels than we also stock plasmids that contain the following promoters in order of decreasing strength TPI (strong), ADHI (medium), STE5 (weak). We also stock Galactose inducible promoter plasmids if inducible expression is required. Please contact us for further information.
Sequence
Please select the file type you require. For reference most cloning programs will import a .gb (Genbank) file and will show all of the plasmids features automatically when downloaded and imported.
Genebank Vector Sequence File,
FASTA Vector Sequence File,
Full Plasmid Map,
Quick-reference Plasmid Map,
Analysis NoteOther NotesLooking for more vector options to move your experiments forward faster? Consider a custom cloning vector designed and built by Oxford Genetics™. Find out more at Oxford Genetics, - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications.Legal InformationOxford Genetics is a trademark of Oxford Genetics LtdПараметрыform buffered aqueous solution mol wt size 6747 bp conjugate 10-His tagged Origin of replication 2Micron, pUC (500 copies) Peptide cleavage no cleavage Peptide tag location C-terminal Promoter Promoter name: TEF1
Promoter activity: constitutive
Promoter type: yeastbacteria selection kanamycin reporter gene none shipped in ambient storage temp. 20°C yeast selection uracil
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-TEF1-COOH-3C-6HIS - C-TERMINAL 6 HIS TAG YEAST PLASMID OGS1923-5UG
NACRES: NA.85 General descriptionPSF-TEF1-COOH-3C-6HIS - C-terminal 6 HIS TAG yeast plasmid is a C-terminal 6His affinity tag yeast expression plasmid. This vector allows the creation of tagged proteins with a 3C cleavage tag.This plasmid is designed to express tagged proteins in yeast cells (Saccharomyces cerevisiae). The plasmid contains an auxotrophic Uracil selection expression cassette (URA3) that allows for the positive selection of yeast that are deficient in the URA3 gene (YEL021W). This is typically achieved by growing the yeast in minimal media that is reconstituted with the essential amino acids and nucleotides but excluding Uracil.
About the Peptide Tag:This plasmid contains a c-terminal Hexa-Histidine (6His) affinity tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: HHHHHH.
About the Cleavage Tag:PSF-TEF1-COOH-3C-6HIS - C-terminal 6 HIS TAG yeast plasmid also encodes a protease cleavage site that is designed to be positioned between your gene of interest and the tag to allow the removal of the tag following protein purification or isolation. This plasmid contains a 3C cleavage tag. The protein sequence of the cleavage tag is: LEVLFQGP. Human Rhinovirus (HRV) 3C Protease is a highly specific protease that cleaves between the Glu and Gly residues of its recognition site. It is often produced with the trademname PreScission protease.
Promoter Expression Level: PSF-TEF1-COOH-3C-6HIS - C-terminal 6 HIS TAG yeast plasmid contains the Yeast Elongation Factor Alpha promoter (TEF1). This is the strongest of the yeast promoters that we sell. It is a constitutive promoter and requires no induction. If you are interested in weaker promoters levels than we also stock plasmids that contain the following promoters in order of decreasing strength TPI (strong), ADHI (medium), STE5 (weak). We also stock Galactose inducible promoter plasmids if inducible expression is required. Please contact us for further information.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 6759 bp conjugate 6-His tagged Origin of replication 2Micron, pUC (500 copies) Peptide cleavage 3C Peptide tag location C-terminal Promoter Promoter name: TEF1
Promoter activity: constitutive
Promoter type: yeastbacteria selection kanamycin reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-TEF1-COOH-3C-BETAGAL-6HIS - C-TERMINAL 6 HIS AND BETA GAL DUAL TAG YEAST PLASMID OGS2033-5UG
NACRES: NA.85 General descriptionThis plasmid is designed to express tagged proteins in yeast cells (Saccharomyces cerevisiae). The plasmid contains an auxotrophic Uracil selection expression cassette (URA3) that allows for the positive selection of yeast that are deficient in the URA3 gene (YEL021W). This is typically achieved by growing the yeast in minimal media that is reconstituted with the essential amino acids and nucleotides but excluding Uracil.
About the Peptide Tag:This plasmid contains a c-terminal Beta Galactosidase reporter tag that can be fused to a gene of interest to allow protein detection. This plasmid also contains a secondary Hexa-Histidine (6His) tag protein tag. The sequence of this tag is: HHHHHHWe provide a range of dual peptide tag plasmids. This is because some peptide tags provide specific biological properties (e. g., small molecule affinity new epitopes solubility or protein secretion) that are not provided by others.
About the Cleavage Tag: This plasmid also encodes a protease cleavage site that is designed to be positioned between your gene of interest and the tag to allow the removal of the tag following protein purification or isolation. This plasmid contains a 3C cleavage tag. The protein sequence of the cleavage tag is: LEVLFQGP. Human Rhinovirus (HRV) 3C Protease is a highly specific protease that cleaves between the Glu and Gly residues of its recognition site. It is often produced with the trademname PreScission protease.
Promoter Expression Level: This plasmid contains the Yeast Elongation Factor Alpha promoter (TEF1). This is the strongest of the yeast promoters that we sell. It is a constitutive promoter and requires no induction. If you are interested in weaker promoters levels than we also stock plasmids that contain the following promoters in order of decreasing strength TPI (strong), ADHI (medium), STE5 (weak). We also stock Galactose inducible promoter plasmids if inducible expression is required. Please contact us for further information.
Sequence
Quick-reference Plasmid Map,
Please select the file type you require. For reference most cloning programs will import a .gb (Genbank) file and will show all of the plasmids features automatically when downloaded and imported.
Genebank Vector Sequence File,
FASTA Vector Sequence File,
Full Plasmid Map,
Analysis NoteOther NotesLooking for more vector options to move your experiments forward faster? Consider a custom cloning vector designed and built by Oxford Genetics™. Find out more at Oxford Genetics, - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications.Legal InformationOxford Genetics is a trademark of Oxford Genetics LtdПараметрыform buffered aqueous solution mol wt size 9900 bp conjugate 6-His tagged, beta-galactosidase tagged Origin of replication 2Micron, pUC (500 copies) Peptide cleavage 3C Peptide tag location C-terminal Promoter Promoter name: TEF1
Promoter activity: constitutive
Promoter type: yeastbacteria selection kanamycin reporter gene beta Gal shipped in ambient storage temp. 20°C yeast selection uracil
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-TEF1-COOH-3C-CMYC - C-TERMINAL CMYC TAG YEAST PLASMID OGS1925-5UG
NACRES: NA.85 General descriptionThis plasmid is designed to express tagged proteins in yeast cells (Saccharomyces cerevisiae). The plasmid contains an auxotrophic Uracil selection expression cassette (URA3) that allows for the positive selection of yeast that are deficient in the URA3 gene (YEL021W). This is typically achieved by growing the yeast in minimal media that is reconstituted with the essential amino acids and nucleotides but excluding Uracil.
About the Peptide Tag:This plasmid contains a c-terminal CMyc epitope tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: EQKLISEEDL.
About the Cleavage Tag:This plasmid also encodes a protease cleavage site that is designed to be positioned between your gene of interest and the tag to allow the removal of the tag following protein purification or isolation. This plasmid contains a 3C cleavage tag. The protein sequence of the cleavage tag is: LEVLFQGP. Human Rhinovirus (HRV) 3C Protease is a highly specific protease that cleaves between the Glu and Gly residues of its recognition site. It is often produced with the trademname PreScission protease.
Promoter Expression Level: This plasmid contains the Yeast Elongation Factor Alpha promoter (TEF1). This is the strongest of the yeast promoters that we sell. It is a constitutive promoter and requires no induction. If you are interested in weaker promoters levels than we also stock plasmids that contain the following promoters in order of decreasing strength TPI (strong), ADHI (medium), STE5 (weak). We also stock Galactose inducible promoter plasmids if inducible expression is required. Please contact us for further information.
Sequence
Quick-reference Plasmid Map,
Please select the file type you require. For reference most cloning programs will import a .gb (Genbank) file and will show all of the plasmids features automatically when downloaded and imported.
Genebank Vector Sequence File,
FASTA Vector Sequence File,
Full Plasmid Map,
Analysis NoteOther NotesLooking for more vector options to move your experiments forward faster? Consider a custom cloning vector designed and built by Oxford Genetics™. Find out more at Oxford Genetics, - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications.Legal InformationOxford Genetics is a trademark of Oxford Genetics LtdПараметрыform buffered aqueous solution mol wt size 6771 bp conjugate c-Myc tagged Origin of replication 2Micron, pUC (500 copies) Peptide cleavage 3C Peptide tag location C-terminal Promoter Promoter name: TEF1
Promoter activity: constitutive
Promoter type: yeastbacteria selection kanamycin reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-TEF1-COOH-3C-CMYC-6HIS - C-TERMINAL 6 HIS AND CMYC DUAL TAG YEAST PLASMID OGS1978-5UG
NACRES: NA.85 General descriptionThis plasmid is designed to express tagged proteins in yeast cells (Saccharomyces cerevisiae). The plasmid contains an auxotrophic Uracil selection expression cassette (URA3) that allows for the positive selection of yeast that are deficient in the URA3 gene (YEL021W). This is typically achieved by growing the yeast in minimal media that is reconstituted with the essential amino acids and nucleotides but excluding Uracil.
About the Peptide Tag:This plasmid contains a c-terminal CMyc epitope tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: EQKLISEEDL. This plasmid also contains a secondary Hexa-Histidine (6His) tag protein tag. The sequence of this tag is: HHHHHHWe provide a range of dual peptide tag plasmids. This is because some peptide tags provide specific biological properties (e. g., small molecule affinity new epitopes solubility or protein secretion) that are not provided by others.
About the Cleavage Tag: This plasmid also encodes a protease cleavage site that is designed to be positioned between your gene of interest and the tag to allow the removal of the tag following protein purification or isolation. This plasmid contains a 3C cleavage tag. The protein sequence of the cleavage tag is: LEVLFQGP. Human Rhinovirus (HRV) 3C Protease is a highly specific protease that cleaves between the Glu and Gly residues of its recognition site. It is often produced with the trademname PreScission protease.
Promoter Expression Level: This plasmid contains the Yeast Elongation Factor Alpha promoter (TEF1). This is the strongest of the yeast promoters that we sell. It is a constitutive promoter and requires no induction. If you are interested in weaker promoters levels than we also stock plasmids that contain the following promoters in order of decreasing strength TPI (strong), ADHI (medium), STE5 (weak). We also stock Galactose inducible promoter plasmids if inducible expression is required. Please contact us for further information.
Sequence
Quick-reference Plasmid Map,
Please select the file type you require. For reference most cloning programs will import a .gb (Genbank) file and will show all of the plasmids features automatically when downloaded and imported.
Genebank Vector Sequence File,
FASTA Vector Sequence File,
Full Plasmid Map,
Analysis NoteOther NotesLooking for more vector options to move your experiments forward faster? Consider a custom cloning vector designed and built by Oxford Genetics™. Find out more at Oxford Genetics, - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications.Legal InformationOxford Genetics is a trademark of Oxford Genetics LtdПараметрыform buffered aqueous solution mol wt size 6789 bp conjugate 6-His tagged, c-Myc tagged Origin of replication 2Micron, pUC (500 copies) Peptide cleavage 3C Peptide tag location C-terminal Promoter Promoter name: TEF1
Promoter activity: constitutive
Promoter type: yeastbacteria selection kanamycin reporter gene none shipped in ambient storage temp. 20°C yeast selection uracil
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену
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С 2010 года мы поставляем оборудование с заводов Европы. Берем на себя все — от подбора оборудования до внедрения на предприятии
Все сотрудники имеют высшее образование, закончили ведущие химические вузы страны, такие как РХТУ им Менделеева.
У большинства компаний срок ожидания составляет 10-12 недель.
Оборудование хранится на сухом отапливаемом складе, где поддерживается ровная температура.
Работаем с PonyExpress и Деловыми линиями. Вы также можете выбрать свою транспортную компанию или забрать товар со склада в Москве.
В случае любых неполадок за свой счет выполним ремонт в сервисном центре или на заводе-изготовителе. Или бесплатно заменим прибор на новый.
Производим пуско-наладку оборудования, валидацию, обучение сотрудников. Если нужно, привлекаем инженеров с заводов- изготовителей.
