StableCell™ MEM M0446-500ML

NACRES:

NA.75

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagles Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.
MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks or Earles salts has broadened the usefulness of this medium.
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
Minimum Essential Medium Eagle has been used in murine fibroblast culture for in vitro cytocompatibility testing.
This product lacks L-Ala; L-Asn; L-Glu; Gly; L-Pro; L-Ser and L-Gln. It is supplemented with L-Ala-L-Gln dipeptide. This provides a more stable form of glutamine for cell culture. Free amino acid L-glutamine is known to be unstable in cell culture.
StableCell is a trademark of Sigma-Aldrich Co. LLC

Quality Level	400,
sterility	sterile-filtered
form	liquid
application(s)	cell culture | mammalian: suitable
impurities	endotoxin, tested
components	phenol red: yes Earle’s salts (5% CO2): no NaHCO3: yes Hanks’ salts (2% CO2): no sodium pyruvate: no glucose: no HEPES: no L-glutamine: no stable glutamine: yes
shipped in	ambient
storage temp.	2-8°C

Personal Protective Equipment	Eyeshields,, Gloves,
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable