Empirical Formula (Hill Notation): | C31H36N2O6 |
CAS Number: | 333973-51-6 |
Molecular Weight: | 532.63 |
Кат. номер |
8521470005 |
8521470025 |
8521470001 |
8.52147.0005 |
8.52147.0001 |
Quasi-orthogonally-protected Lys derivative. The Fmoc group can be removed selectively by treatment with piperidine; the Dde group is cleaved with 2% hydrazine in DMF [1]. When removing Dde in the presence of allyl based protecting groups, allyl alcohol should be included in the deprotection solution to prevent reduction of the allyl group [2].
Lys(Dde) has been employed in the following applications: synthesis of branched peptides [1] and di-epitopic peptides [3]; preparation of MAP core molecules and lipo-MAPs [4, 5]; construction of cyclic peptides [6], TASP molecules [7], templates for combinatorial chemistry [8] and synthetic proteins [9]; preparation of peptides modified at the lysine side-chain [10-14].
It has been reported that Dde can migrate from the side-chain of Lys to the unprotected side-chain of another Lys residue [15], and from the -amino group to the -amino group of Dpr [16]. In the former instance, this problem can be overcome by using Fmoc-Lys(ivDde)-OH (852082) or using DBU/DMF (2:98) for Fmoc group removal [15, 17].
Full orthogonality of Dde with Fmoc has recently been demonstrated when hydroxylamine is used for Dde removal [18].
The product number for this product was previously 04-13-1028.
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Replaces: 04-13-1028
Color (visual): white to slightly yellow to beige
Appearance of substance (visual): powder
Identity (IR): passes test
Enantiomeric purity: 99.5 % (a/a)
Purity (TLC(157B)): 98 %
Purity (TLC(CMA2)): 98 %
Assay (HPLC, area%): 98.0 % (a/a)
Solubility (1mmole in 2 ml DMF): clearly soluble
Assay (acidimetric): 95.0 %
Water (K. F.): 1.00 %
For TLC-Systems see our General Catalog
Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany
storage conditions | Store at -15°C to -25°C. |
Quality Level | 200, |
product line | Novabiochem® |
assay | 95.0% (acidimetric), 98% (TLC), 98.0% (HPLC) |
form | powder |
reaction suitability | reaction type: Fmoc solid-phase peptide synthesis |
Торговая марка | Novabiochem® |
application(s) | peptide synthesis: suitable |
mp | 135-145 °C |
functional group | Fmoc |
storage temp. | 15-25°C |