Cell Lines & Specialty Cell Culture
- Сортировать:
- Вид таблицей
-
BHK 21 STRAIN 35
Кат. номер 93120841-DNA-5UG 93120841-1VL 93120841-RNA-5UG Cell Line OriginHamster Syrian kidneyCell Line DescriptionUsed extensively for the production of foot and mouth disease virus.ApplicationVirus studiesCulture MediumGMEM + 2mM Glutamine + 10% Tryptose Phosphate Broth + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source hamster kidney growth mode Adherent karyotype Not specified morphology Epithelial products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BHK 21 STRAIN 38
Кат. номер 93120842-DNA-5UG 93120842-1VL 93120842-RNA-5UG Cell Line OriginHamster Syrian kidneyCell Line DescriptionUsed in the production of foot and mouth disease virus and pseudorabies virus.ApplicationVirus studiesCulture MediumGMEM + 2mM Glutamine + 10% Tryptose Phosphate Broth + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source hamster kidney growth mode Adherent karyotype Not specified morphology Epithelial products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BHK TK-
Кат. номер 85011423-RNA-5UG 85011423-DNA-5UG 85011423-1VL Cell Line OriginHamster Syrian kidneyCell Line DescriptionBHK TK- is a derivative of BHK21 (clone 13) rendered TK- by selection in 30μg/ml BUdR. Used widely for research on viruses and on neoplastic transformation especially with polyoma virus.ApplicationTransformation and virus studiesCulture MediumDMEM or GMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source hamster kidney growth mode Adherent karyotype Not specified morphology Fibroblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BHK/AC9
Кат. номер 85040103-1VL 85040103-RNA-5UG 85040103-DNA-5UG Cell Line OriginHamster Syrian kidneyCell Line DescriptionBHK/AC9 is derived from the parent line BHK-21 (Clone-13) (Sigma Catalogue number. 85011433). The cells have been adapted to grow in suspension and have the same virus susceptibility as the parent line.ApplicationVirus replication studiesCulture MediumGMEM + 2mM Glutamine + 5% Tryptose Phosphate Broth + 5-10% Foetal Bovine Serum (FBS).Subculture RoutineMaintain cultures between 2-9x100,000 cells/ml; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source hamster kidney growth mode Suspension karyotype 2n = 44, pseudodiploid morphology Not specified products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BHK21-Hektor
Кат. номер 5062301-DNA-5UG 5062301-1VL Cell Line OriginHamster Syrian kidney, serum-freeCell Line DescriptionBHK21CL13-2P cell line (Sigma Catalogue number. 84111301) adapted to grow in the chemically defined protein- and peptide-free Hektor™ G medium.Culture MediumHektor™ G medium + 4mM Glutamine + 5 ug/ml Insulin. When freezing cells down use Hektor G medium + 4mM Glutamine (50:50 conditioned medium:fresh medium) + 10% DMSO.Subculture RoutineViability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. TranOther NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Legal InformationHektor is a trademark of Cell Culture TechnologiesПараметрыbiological source hamster kidney growth mode Suspension karyotype Not specified morphology Fibroblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BHK21-InVitrus
Кат. номер 5062302-1VL 5062302-DNA-5UG Cell Line OriginHamster Syrian kidney, serum-freeCell Line DescriptionBHK21CL13-2P cell line (Sigma Catalogue number. 84111301) adapted to grow in the chemically defined protein- and peptide-free InVitrus™ medium.Culture MediumInVitrus™ medium + 4mM Glutamine + 5 ug/ml Insulin. When freezing cells down use InVitrus medium + 4mM Glutamine (50:50 conditioned medium:fresh medium) + 10% DMSO.Subculture RoutineViability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. TranOther NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Legal InformationInVitrus is a trademark of Cell Culture TechnologiesПараметрыbiological source hamster kidney growth mode Suspension karyotype Not specified morphology Fibroblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BHK21C13-2P
Кат. номер 84111301-RNA-5UG 84111301-DNA-5UG 84111301-1VL Cell Line OriginHamster Syrian kidneyCell Line DescriptionDerived from the parent line BHK-21 (Clone-13) (Sigma Catalogue number. 85011433). The cells have been adapted to growth in suspension and have the same virus susceptibility as the parent line.ApplicationVirus replication studiesCulture MediumGMEM + 2mM Glutamine + 5% Tryptose Phosphate Broth + 10% Foetal Bovine Serum (FBS).Subculture RoutineMaintain cultures between 2-9x100,000 cells/ml (split ratio of 1:3 to 1:6); 5% CO2; 37°C. Cells need to be grown in a techni-stirrer or they may start to re-attach.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source hamster kidney growth mode Suspension karyotype 2n = 44, pseudodiploid morphology Not specified products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BHK21C13-3P
Кат. номер 84100501-DNA-5UG 84100501-RNA-5UG 84100501-1VL Cell Line OriginHamster Syrian kidneyCell Line DescriptionBHK21C13-3P is a subclone of the parent line BHK-21 (Clone-13) (Sigma Catalogue number. 85011433). The cells grow as monolayers and have the same virus susceptibility as the parent line.ApplicationVirus replication studiesCulture MediumGMEM + 2mM Glutamine + 5% Tryptose Phosphate Broth + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source hamster kidney growth mode Adherent karyotype 2n = 44, pseudodiploid morphology Fibroblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BHL-89
Кат. номер 98070109-RNA-5UG 98070109-DNA-5UG 98070109-CDNA-20UL 98070109-1VL Cell Line OriginHuman B-type EBNA negative lymphomaCell Line DescriptionThe cell line BHL-89 was established from a 56 year old female patient with chronic lymphoid leukaemia (CLL). The B-cell type cells were shown to express CD9, CD22, CD71,HLA-ABC, BB1, GnRH and surface IgM. Cloning efficiency in soft agar was found to be between 20-60% and population doubling time was 48 hours. BHL-89 cells are Epstein-Barr nuclear antigen (EBNA) negative. They have been shown to be tumourigenic in immunosuppressed mice, sensitive to dibromodulcitol and resistant to the phorbol ester TPA. The cell line was found to be positive for BCL-2 protein and produces small amounts of interferon. Lymphoblastoid cells and floating aggregates. The cell line has been eradicated from mycoplasma at ECACC.ApplicationB lymphoma studies, cancer treatmentsCulture MediumRPMI1640 + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).Subculture RoutineMaintain cultures between 1-5 x 100,000 cells/ml;5% CO2; 37°COther NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human lymph (axillary) growth mode Suspension karyotype Hyperdiploid, modal no. 47, chromosome 1 trisomy, 15 monosomy, +14q+ morphology Lymphoblastoid products Not specified receptors sIgM,CD22,BB1,HLA-ABC,GnRH application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BICR 10
Кат. номер 4072103-RNA-5UG 4072103-1VL 4072103-DNA-5UG 4072103-CDNA-20UL Cell Line OriginBuccal mucosa squamous carcinomaCell Line DescriptionAdherent keratinocyte cell line derived from a recurrent squamous cell carcinoma of the buccal mucosa of a Caucasian female. Keratin and involucrin markers present. Known mutations: p53, p16 and p14ARF. Cells are tumourigenic in athymic mice.ApplicationStudy of genotype/phenotype relationshipsCulture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.4 micrograms/ml Hydrocortisone.Subculture RoutineSplit sub-confluent cultures (70-80%) 1:10 - 1:50 using 0.25% trypsin/EDTA; 8% CO2; 37 °C. Suggested seeding density 2 x 10,000 cells/cm2.
Culture cells on a feeder layer of lethally-irradiated or mitomycin C-treated 3T3 Swiss Albino cells (Sigma catalog number 85022108). Feeder layers are prepared in the flasks at least 24 hours in advance of being required. Where frozen stocks of treated 3T3 Swiss Albino cells have been prepared, an ampoule is thawed in 37 °C water bath and the contents quickly transferred to a 15 ml centrifuge tube. DMEM medium is added drop wise to 5 ml. Cells are centrifuged at 150 x g for 5 min at room temperature. Cells are resuspended in 5 ml of medium. Cells are counted and added to flasks containing the correct BICR growth medium at 1-3 x 104 cells/cm2.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.Параметрыbiological source human mouth growth mode Adherent karyotype Aneuploid morphology Epithelial, keratinocyte products Not specified receptors Epidermal Growth Factor Receptor (EGFR) application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BICR 16
Кат. номер 6031001-RNA-5UG 6031001-CDNA-20UL 6031001-1VL Cell Line OriginTongue squamous carcinomaCell Line DescriptionAdherent cell line derived from a recurrent squamous cell carcinoma of the tongue of a Caucasian male. Keratin and involucrin markers present. Known mutations: p53, p16 and p14ARF. Cells are tumourigenic in athymic mice.Culture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.4 micrograms/ml Hydrocortisone.Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 using 0.25% trypsin/EDTA; 8% CO2; 37°C. Suggested seeding density 2-4 x 10,000 cells/cm2. A feeder layer of lethally-irradiated or mitomycin C-treated 3T3 feeder cells can be used to culture this cell line, howevOther NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.Параметрыbiological source human mouth growth mode Adherent karyotype Aneuploid morphology Epithelial, keratinocyte products Not specified receptors Epidermal Growth Factor Receptor (EGFR) application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
BICR 18
Кат. номер 6051601-RNA-5UG 6051601-1VL 6051601-CDNA-20UL Cell Line OriginLarynx squamous cell carcinoma (lymph node metastasis)Cell Line DescriptionAdherent keratinocyte cell line derived from a lymph node metastasis squamous cell carcinoma (SSC) of the larynx. Keratin and involucrin markers present. Known mutations: p53, p16 and p14ARF. Cells are tumourigenic in athymic mice. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line. It is a known phenomenon that SSC cell lines can lose their Y chromosome.Culture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.4 micrograms/ml Hydrocortisone.Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 using 0.25% trypsin/EDTA; 8% CO2; 37 C. Suggested seeding density 4 x 10,000 cells/cm2. A feeder layer of lethally-irradiated or mitomycin C-treated 3T3 feeder cells can be used to culture this cell line, howeveOther NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.Параметрыbiological source human larynx growth mode Not specified karyotype Aneuploid morphology Epithelial products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) metastasis shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
BICR 22
Кат. номер 4072106-DNA-5UG 4072106-CDNA-20UL 4072106-1VL 4072106-RNA-5UG Cell Line OriginTongue squamous carcinoma (lymph node metastasis)Cell Line DescriptionAdherent cell line derived from a lymph node metastasis squamous cell carcinoma of the tongue of a Caucasian male. Keratin and involucrin markers present. Known mutations: p53, p16 and p14ARF. Cells are tumourigenic in athymic mice.ApplicationStudy of genotype/phenotype relationshipsCulture MediumDMEM + 2mM Glutamine + 2% Foetal Bovine Serum (FBS) + 0.4 micrograms/ml Hydrocortisone.Subculture RoutineSplit sub-confluent cultures (70-80%) 1:10 - 1:50 using 0.25% trypsin/EDTA; 8% CO2; 37 °C. Suggested seeding density 2 x 10,000 cells/cm2.
Culture cells on a feeder layer of lethally-irradiated or mitomycin C-treated 3T3 Swiss Albino cells (Sigma catalog number 85022108). Feeder layers are prepared in the flasks at least 24 h in advance of being required. Where frozen stocks of treated 3T3 Swiss Albino cells have been prepared, an ampoule is thawed in 37 °C water bath and the contents quickly transferred to a 15 ml centrifuge tube. DMEM medium is added drop wise to 5 ml. Cells are centrifuged at 150 x g for 5 min at room temperature. Cells are resuspended in 5 ml of medium. Cells are counted and added to flasks containing the correct BICR growth medium at 1-3 x 104 cells/cm2.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.Параметрыbiological source human mouth growth mode Adherent karyotype Aneuploid morphology Epithelial, keratinocyte products Not specified receptors Epidermal Growth Factor Receptor (EGFR) application(s) cell culture | mammalian: suitable relevant disease(s) metastasis shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BICR 3
Кат. номер 6032203-RNA-5UG 6032203-1VL 6032203-DNA-5UG 6032203-CDNA-20UL Cell Line OriginAlveolus squamous cell carcinomaCell Line DescriptionAdherent keratinocyte cell line derived from a squamous cell carcinoma of the alveolus of a Caucasian female. Keratin and involucrin markers present. Known mutations: p53 and p16. Cells are tumourigenic in athymic mice. Epidermal Growth Factor Receptor (EGFR) present.Culture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.4 micrograms/ml Hydrocortisone.Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 using 0.25% trypsin/EDTA; 8% CO2; 37 C. Suggested seeding density 4 x 10,000 cells/cm2. A feeder layer of lethally-irradiated or mitomycin C-treated 3T3 feeder cells can be used to culture this cell line, howeveOther NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.Параметрыbiological source human mouth growth mode Adherent karyotype Aneuploid, Modal no. 49 morphology Epithelial, keratinocyte products Not specified receptors Epidermal Growth Factor Receptor (EGFR) application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BICR 31
Кат. номер 4072107-1VL 4072107-CDNA-20UL 4072107-DNA-5UG 4072107-RNA-5UG Cell Line OriginTongue squamous carcinomaCell Line DescriptionAdherent cell line derived from a squamous cell carcinoma of the tongue of a Caucasian male. Keratin and involucrin markers present. Known mutations: p53, p16 and p14ARF. Cells are tumourigenic in athymic mice.ApplicationStudy of genotype/phenotype relationshipsCulture MediumDMEM + 2mM Glutamine + 2% Foetal Bovine Serum (FBS) + 0.4 micrograms/ml Hydrocortisone.Subculture RoutineSplit sub-confluent cultures (70-80%) 1:10 - 1:50 using 0.25% trypsin/EDTA; 8% CO2; 37 °C. Suggested seeding density 2 x 10,000 cells/cm2.
Culture cells on a feeder layer of lethally-irradiated or mitomycin C-treated 3T3 Swiss Albino cells (Sigma catalog number 85022108). Feeder layers are prepared in the flasks at least 24 h in advance of being required. Where frozen stocks of treated 3T3 Swiss Albino cells have been prepared, an ampoule is thawed in 37 °C water bath and the contents quickly transferred to a 15 ml centrifuge tube. DMEM medium is added drop wise to 5 ml. Cells are centrifuged at 150 x g for 5 min at room temperature. Cells are resuspended in 5 ml of medium. Cells are counted and added to flasks containing the correct BICR growth medium at 1-3 x 104 cells/cm2.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.Параметрыbiological source human mouth growth mode Adherent karyotype Pseudodiploid/Aneuploid morphology Epithelial, keratinocyte products Not specified receptors Epidermal Growth Factor Receptor (EGFR) application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BICR 56
Кат. номер 6031002-1VL 6031002-CDNA-20UL 6031002-DNA-5UG 6031002-RNA-5UG Cell Line OriginTongue squamous cell carcinomaCell Line DescriptionAdherent keratinocyte cell line derived from a squamous cell carcinoma of the tongue of a Caucasian female. Keratin and involucrin markers present. Known mutations: p53 and p16. Cells are tumourigenic in athymic mice. Epidermal Growth Factor Receptor (EGFR) present.Culture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.4 micrograms/ml Hydrocortisone.Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 - 1:4 using 0.25% trypsin/EDTA; 8% CO2; 37 C. Suggested seeding density 4 x 10,000 cells/cm2. A feeder layer of lethally-irradiated or mitomycin C-treated 3T3 feeder cells can be used to culture this cell line,Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.Параметрыbiological source human mouth growth mode Adherent karyotype Aneuploid, Modal no. 86 morphology Epithelial, keratinocyte products Not specified receptors Epidermal Growth Factor Receptor (EGFR) application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BICR 6
Кат. номер 5070501-DNA-5UG 5070501-1VL 5070501-RNA-5UG 5070501-CDNA-20UL Cell Line OriginHypopharynx squamous cell carcinomaCell Line DescriptionAdherent keratinocyte cell line derived from a squamous cell carcinoma of the hypopharynx of a Caucasian male. Keratin and involucrin markers present. Known mutations: p53, p16 and p14ARF. Cells are tumourigenic in athymic mice. Epidermal Growth Factor Receptor (EGFR) present.Culture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.4 micrograms/ml Hydrocortisone.Subculture RoutineSplit sub-confluent cultures (70-80%) 1:10 - 1:50 using 0.25% trypsin/EDTA; 8% CO2; 37 C. Suggested seeding density 2 x 10,000 cells/cm2. Culture cells on a feeder layer of lethally-irradiated or mitomycin C-treated 3T3 feeder cells.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.Параметрыbiological source human pharynx growth mode Adherent karyotype Aneuploid morphology Epithelial, keratinocyte products Not specified receptors Epidermal Growth Factor Receptor (EGFR) application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BICR 78
Кат. номер 4072111-DNA-5UG 4072111-1VL 4072111-RNA-5UG 4072111-CDNA-20UL Cell Line OriginOral alveolus squamous carcinomaCell Line DescriptionAdherent keratinocyte cell line derived from a squamous cell carcinoma of the oral alveolus of a Caucasian male. Keratin and involucrin markers present. Known mutations: p53, p16 and p14ARF. Cells are not tumourigenic in athymic mice.ApplicationStudy of genotype/phenotype relationshipsCulture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.4 micrograms/ml Hydrocortisone.Subculture RoutineSplit sub-confluent cultures (70-80%) 1:10 - 1:50 using 0.25% trypsin/EDTA; 8% CO2; 37 °C. Suggested seeding density 2 x 10,000 cells/cm2.
Culture cells on a feeder layer of lethally-irradiated or mitomycin C-treated 3T3 Swiss Albino cells (Sigma catalog number 85022108). Feeder layers are prepared in the flasks at least 24 h in advance of being required. Where frozen stocks of treated 3T3 Swiss Albino cells have been prepared, an ampoule is thawed in 37 °C water bath and the contents quickly transferred to a 15 ml centrifuge tube. DMEM medium is added drop wise to 5 ml. Cells are centrifuged at 150 x g for 5 min at room temperature. Cells are resuspended in 5 ml of medium. Cells are counted and added to flasks containing the correct BICR growth medium at 1-3 x 104 cells/cm2.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.Параметрыbiological source human mouth growth mode Adherent karyotype Aneuploid morphology Epithelial, keratinocyte products Not specified receptors Epidermal Growth Factor Receptor (EGFR) application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BICR 82
Кат. номер 4072112-CDNA-20UL 4072112-1VL 4072112-RNA-5UG 4072112-DNA-5UG Cell Line OriginHuman Upper Jaw (Maxilla) CarcinomaCell Line DescriptionA keratinocyte cell line established from a squamous carcinoma of the upper jaw (maxilla) of a Caucasian male. Keratin and involucrin markers present. Known mutations: p53, p16 and p14ARF. Tumourigenicity in athymic mice not tested. Edington et al., 1995 must be cited and the cell line referred to by its original name.Culture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.4 micrograms/ml Hydrocortisone.Subculture RoutineSplit sub-confluent cultures (70-80%) 1:4 - 1:5 using 0.25% trypsin/EDTA; 8% CO2; 37°C. Suggested seeding density 2 x 10,000 cells/cm2. Culture cells on a feeder layer of lethally-irradiated or mitomycin C-treated 3T3 feeder cells.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.Параметрыbiological source human mouth (maxilla) growth mode Adherent karyotype Aneuploid morphology Epithelial products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BIN40 human cell line
Кат. номер 86112010-DNA-5UG 86112010-1VL 86112010-CDNA-20UL 86112010-RNA-5UG General descriptionHomozygous: YesCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileHLA DRB1: 404
HLA DQB1: 302Serological ProfileHLA Dw: 14.1
HLA DR: 4Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Not specified gender not specified growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
BJ human cell line
Кат. номер 94022524-DNA-5UG 94022524-RNA-5UG 94022524-1VL 94022524-CDNA-20UL General descriptionHomozygous: No
Consanguineous: NoCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileA: 6901 3001
Cw: 1701 1203
B: 4201 3508
HLA DRB1: 15 11
HLA DQB1: 06 03Serological ProfileHLA A: 30 69
HLA B: 22 35
Bw4/bw6: 6Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Not specified gender male growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
BL-3
Кат. номер 86062401-RNA-5UG 86062401-1VL 86062401-DNA-5UG Cell Line OriginBovine leukaemic lymphoblastCell Line DescriptionBL-3 was established from a neoplastic lymph node of a 3 month old male calf. BVDV positive.ApplicationVirus studiesCulture MediumEMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Foetal Bovine Serum (FBS).Subculture RoutineMaintain cultures between 5-9x100,000 cells/ml; 5% CO2; 37°C. Cells should not be over diluted.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source bovine blood growth mode Suspension karyotype 2n = 60, aneuploid, modal no. 65 morphology Lymphoblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BLJU human cell line
Кат. номер 94071408-CDNA-20UL 94071408-DNA-5UG 94071408-RNA-5UG 94071408-1VL General descriptionHomozygous: No
Consanguineous: NoCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileHLA DRB1: 302
HLA DQA1: 401
HLA DQB1: 0402 0602Serological ProfileHLA A: 2 19
HLA B: 44 50
HLA Dw: 2 -
HLA DR: 18 2
HLA DQ: 1 4Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity African American gender not specified growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
BLO-11
Кат. номер 90112702-1VL 90112702-RNA-5UG 90112702-DNA-5UG Cell Line OriginMouse Moblo/y muscle hereditary emphysemaCell Line DescriptionEstablished from the abdominal wall muscle of a 30 day old male Blotchy mouse (Mo (blo/y)) with hereditary emphysema. The cells are deficient in lysyl oxidase activity and accumulate high levels of intracellular copper. NOR-10 (Sigma Catalogue number. 90112701) was isolated from the abdominal wall muscle of a normal litter mate of the mouse from which BLO-11 was derived, thus it can serve as a matched control for studies with BLO fibroblasts.ApplicationGenetic studiesCulture MediumDMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse muscle growth mode Adherent karyotype 2n = 40 morphology Fibroblast-like products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BM14 human cell line
Кат. номер 88052033-DNA-5UG 88052033-1VL 88052033-CDNA-20UL 88052033-RNA-5UG General descriptionConsanguineous: YesCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileA: 301
Cw: 702
B: 702
HLA DRA: 101
HLA DRB1: 401
HLA DRB4: 101
HLA DQA1: 3
HLA DQB1: 302
HLA DPA1: 1
HLA DPB1: 401Serological ProfileHLA A: 3
HLA A ief: 3.2
HLA C: 7
HLA B: 7
HLA B ief: 7.2
Bw4/bw6: 6
HLA Dw: 4
HLA DR: 4
HLA DR53: 53
HLA DQ: 8
HLA DP: 4Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Italian gender male growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
BM15 human cell line
Кат. номер 88052040-RNA-5UG 88052040-CDNA-20UL 88052040-1VL 88052040-DNA-5UG General descriptionConsanguineous: YesCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileA: 101
Cw: 701
B: 4901
HLA DRA: 101
HLA DRB1: 1102
HLA DRB3: 202
HLA DQAP: 4.1
HLA DQA1: 501
HLA DQB1: 301
HLA DPA1: 1
HLA DPB1: 301Serological ProfileHLA A: 1
HLA A ief: 1
HLA C: 7
HLA B: 49
HLA B ief: 49.2
Bw4/bw6: 4
HLA Dw: 5
HLA DR: 11
HLA Dw2: 25
HLA DR52: 52
HLA DQ: 7
HLA DP: 3Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Italian gender male growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
BM16 human cell line
Кат. номер 88052038-DNA-5UG 88052038-CDNA-20UL 88052038-RNA-5UG 88052038-1VL General descriptionConsanguineous: YesCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileA: 201
Cw: 701
B: 1801
HLA DRA: 102
HLA DRB1: 1201
HLA DRB3: 202
HLA DQAP: 4.1
HLA DQA1: 5013
HLA DQB1: 301
HLA DPA1: 1
HLA DPB1: 2012Serological ProfileHLA A: 2
HLA A ief: 2.2
HLA C: 7
HLA B: 18
HLA B ief: 18.1
Bw4/bw6: 6
HLA Dw: DB6
HLA DR: 12
HLA Dw2: 25
HLA DR52: 52
HLA DQ: 7
HLA DP: 2Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Italian gender female growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
BM21 human cell line
Кат. номер 88052043-DNA-5UG 88052043-CDNA-20UL 88052043-RNA-5UG 88052043-1VL General descriptionConsanguineous: YesCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileA: 101
Cw: 1701
B: 4101
HLA DRA: 101
HLA DRB1: 1101
HLA DRB3: 202
HLA DQA1: 501
HLA DQB1: 301
HLA DPA1: 201
HLA DPB1: 1001Serological ProfileHLA A: 1
HLA A ief: 1
HLA B: 41
HLA B ief: 41.1
Bw4/bw6: 6
HLA Dw: 5
HLA DR: 11
HLA Dw2: 25
HLA DR52: 52
HLA DQ: 7Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity German/Italian gender male growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
BM9 human cell line
Кат. номер 88052086-DNA-5UG 88052086-CDNA-20UL 88052086-1VL 88052086-RNA-5UG General descriptionConsanguineous: YesCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileA: 201
Cw: 401
B: 3501
HLA DRA: 102
HLA DRB1: 801
HLA DQA1: 401
HLA DQB1: 402
HLA DPA1: 1
HLA DPB1: 2012Serological ProfileHLA A: 2
HLA A ief: 2.2
HLA C: 4
HLA B: 35
HLA B ief: 35.3
Bw4/bw6: 6
HLA Dw: 8.1
HLA DR: 8
HLA DQ: 4
HLA DP: 2Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Italian gender female growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
BMAC-3
Кат. номер 89062105-DNA-5UG 89062105-RNA-5UG 89062105-1VL Cell Line OriginMouse NS0 x Mouse BALB/c spleenCell Line DescriptionThe antibody recognises the human leukocyte common antigen CD45, determinant 2a.ImmunogenPurified L-CA from the Daudi cell lineCulture MediumRPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitationOther NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon BALB/c mouse spleen Myeloma NS0 Reactivity Anti-CD45 determinant 2a, human application(s) cell culture | mammalian: suitable isotype IgG1 shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Bob cell line human 10021102-1VL
Cell Line OriginHuman prostate cancer spontaneously immortalized, serum-freeCell Line DescriptionFirst spontaneously immortalized prostate cancer cell line to be established from a trans-rectal needle biopsy (TRBP) of a patient with castration-resistant prostate cancer (CRPC). Bob is a novel pre-clinical model for functional studies in CRPC and especially for studying the CRPC "basal" phenotype. This cell line is cultured in serum-free culture medium, a derivative of Bob is also available i.e. SerBob, Sigma Catalogue number 10021101, which has been adapted to medium containing serum. SerBob is more differentiated and invasive than Bob. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.ApplicationProstate cancer research including drug testing.
For complete product information, please see ECACC,DNA ProfileSTR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 9,14
D16S539: 10,12
D5S818: 13
D7S820: 10,11
THO1: 9
TPOX: 8,10
vWA: 17Culture MediumKeratinocyte-SFM supplied with prequalified human recombinant epidermal growth factor 1-53 (EGF 1-53), bovine pituitary extract (BPE) and glutamine) + 2ng/ml leukaemia inhibitory factor, 2ng/ml stem cell factor + 100ng/ml cholera toxin + 1ng/ml granulocyte macrophage colony stimulating factor.Subculture RoutineSplit sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 2-4 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cells must be centrifuged at 400 x g for 5 minutes to remove the trypsin at each subculture. Cells are cryopreserved in 45% conditioned media: 45% fresh media: 10% DMSO. At confluence 5-7 x 104 cells/cm2 can be expected.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply, for more information.Параметрыbiological source human prostate Quality Level 100, growth mode Adherent karyotype 4n (81 to 94) morphology Cobblestone products 10% 3-D sphere formation in Matrigel. Migratory and invasive when compared to benign prostate tumour. receptors CD44, alpha2 integrin, alpha6 integrin, EGFR, FGFR1, LIFR application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
BOB human cell line
Кат. номер 88052089-CDNA-20UL 88052089-1VL 88052089-DNA-5UG 88052089-RNA-5UG General descriptionHomozygous: YesCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileA: 2402 (!)2403/33
Cw: 1502
B: 5101
HLA DRA: 101
HLA DRB1: 1104
HLA DRB3: 202
HLA DQA1: 501
HLA DQB1: 301
HLA DPA1: 1
HLA DPB1: 402Serological ProfileHLA A: 24
HLA A ief: 24.1
HLA B: 51
HLA B ief: 51
Bw4/bw6: 4
HLA DR: 11
HLA Dw2: 25
HLA DR52: 52
HLA DQ: 7
HLA DP: 4Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity German gender female growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
BOLETH human cell line
Кат. номер 88052031-CDNA-20UL 88052031-DNA-5UG 88052031-1VL 88052031-RNA-5UG General descriptionConsanguineous: YesCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileA: 201
Cw: 304
B: 1501
HLA DRA: 101
HLA DRB1: 401
HLA DRB4: 103
HLA DQA1: 3011
HLA DQB1: 302
HLA DPA1: 103
HLA DPB1: 401Serological ProfileHLA A: 2
HLA A ief: 2.2
HLA C: 10
HLA B: 62
HLA B ief: 62.3
Bw4/bw6: 6
HLA Dw: 4
HLA DR: 4
HLA DR53: 53
HLA DQ: 8
HLA DP: 4Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Swedish gender male growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
BON-CT human cell line
Кат. номер 94071429-DNA-5UG 94071429-CDNA-20UL 94071429-1VL 94071429-RNA-5UG General descriptionHomozygous: No
Consanguineous: NoCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileA: 01 03
Cw: 1601 04
B: 44031 35
HLA DRB1: 103
HLA DQA1: 101Serological ProfileHLA A: 1 3
HLA C: 4
HLA B: 44 35
Bw4/bw6: 4 6
HLA Dw: BON
HLA DR: 103
HLA DQ: 5
HLA DP: 4Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity French gender male growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
BONA human cell line
Кат. номер 94050304-CDNA-20UL 94050304-1VL 94050304-DNA-5UG 94050304-RNA-5UG General descriptionHomozygous: No
Consanguineous: NoCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileA: 02 03
B: 51
HLA DRB1: 1101 1604
HLA DRB3: 2
HLA DRB5: 2
HLA DRB6: 202
HLA DQA1: 0501 0102
HLA DQB1: 0301 0502
HLA DPB1: 0401 0301Serological ProfileHLA A: 3 2
HLA B: 51 51
Bw4/bw6: 4HLA DR: 11 16
HLA DR52: 52
HLA DQ: 7 5Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity French Caucasian gender male growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
BOW, MF human cell line
Кат. номер 94082250-CDNA-20UL 94082250-RNA-5UG 94082250-1VL 94082250-DNA-5UG General descriptionHomozygous: No
Consanguineous: NoCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileA: 02
B: 50 44
HLA DRB1: 0401 07
HLA DRB4: 101
HLA DQAP: 2.1 3.1
HLA DQA1: 0201 03
HLA DQB1: 0301 0201
HLA DPB1: 0401 0402Serological ProfileHLA A: 2 2
HLA A ief: 2.1 2.2
HLA C: 5
HLA C wief:
HLA B: 44 21Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Not specified gender female growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
BPAEC
Кат. номер 86123101-DNA-5UG 86123101-RNA-5UG 86123101-1VL Cell Line OriginBovine lung endotheliumCell Line DescriptionBPAEC is susceptible to bovine respiratory viruses and is positive for BVDV.ApplicationVirus studies: bovine respiratory virusesCulture MediumDMEM or 199 + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source bovine lung growth mode Adherent karyotype Not specified morphology Endothelial products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BRIN-BD11
Кат. номер 10033003-DNA-5UG 10033003-1VL 10033003-RNA-5UG Cell Line OriginA rat pancreatic Beta cell: RINm5F hybrid cell line. Insulin-secreting cell lineCell Line DescriptionBRIN-BD11 is a hybrid cell line formed by the electrofusion of a primary culture of NEDH rat pancreatic islets with RINm5F (a cell line derived from a NEDH rat insuliinoma). BRIN-BD11 has been shown to be tumourigenic when transplanted into a SCID mouse host. BRIN-BD11 has been mycoplasma eradicated at ECACC and the stocks available for supply have undergone a further 10 passages without detection of mycoplasma. The cell line has applications in the study of pancreatic beta cell function.DNA ProfileNot specifiedCulture MediumRPMI-1640 + 2 mM Glutamine + 10% FCS / FBSSubculture RoutineSplit sub-confluent cultures (70-80%) 1:8 to 1:10 , i.e., seeding at 2-4 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C. Population doubling approx 20hrs. At confluence 105 cells/cm2 can be expected.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.Параметрыbiological source rat pancreas (islets) growth mode Not specified karyotype Modal chromosome number 58 over 50 passages morphology Epitheloid products BRIN-BD11 is a stable, glucose-responsive insulin secreting beta cell line expressing insulin, glucokinase and IAPP as detected by immunocytochemistry. BRIN-BD11 cells express the GLUT2 glucose transporter. receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
BRIN-BG5
Кат. номер 10033001-DNA-5UG 10033001-RNA-5UG 10033001-1VL Cell Line OriginA rat pancreatic Beta cell: RINm5F hybrid cell line. Insulin-secreting cell lineCell Line DescriptionBRIN-BG5 is a hybrid cell line formed by the electrofusion of a primary culture of NEDH rat pancreatic islets with RINm5F (a cell line derived from a NEDH rat insuliinoma). BRIN-BG5 has been shown to be tumourigenic when transplanted into a SCID mouse host. BRIN-BG5 has been mycoplasma eradicated at ECACC and the stocks available for supply have undergone a further 10 passages without detection of mycoplasma.Culture MediumRPMI 1640, (R0883) + 2 mM L-Glutamine, (G7513) + FBS / FCS, (F2442)Subculture RoutineSplit sub-confluent cultures (70-80%) 1:8 to 1:10, i.e. seeding at 2-4 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C. Population doubling approximately28hrs. At confluence 105 cells/cm2 can be expected.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.Параметрыbiological source rat pancreas (islets) growth mode Adherent karyotype Modal chromosome number 66 up to passage 50 morphology Epitheloid products BRIN-BG5 is a stable, glucose-responsive insulin secreting beta cell line expressing insulin, glucokinase and IAPP as detected by immunocytochemistry. BRIN-BG5 cells express the GLUT2 glucose transporter. receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
BRIN-BG7
Кат. номер 10020902-DNA-5UG 10020902-1VL 10020902-RNA-5UG Cell Line OriginA rat pancreatic Beta cell: RINm5F hybrid cell line. Insulin-secreting cell lineDNA ProfileNot specifiedCulture MediumRPMI-1640 + 2 mM Glutamine + 10% FCS / FBSSubculture RoutineSplit sub-confluent cultures (70-80%) 1:8 to 1:10 , i.e., seeding at 2-4 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C. Population doubling approx 20hrs. At confluence 105 cells/cm2 can be expected.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.Параметрыbiological source rat pancreas (islets) growth mode Adherent karyotype Modal chromosome number 66 up to passage 50 morphology Epitheloid products BRIN-BG7 is a stable, glucose-responsive insulin secreting beta cell line expressing insulin, glucokinase and IAPP as detected by immunocytochemistry. BRIN-BG7 cells express the GLUT2 glucose transporter. receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
BRIP human cell line
Кат. номер 88052044-DNA-5UG 88052044-RNA-5UG 88052044-CDNA-20UL 88052044-1VL General descriptionConsanguineous: YesCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileA: 2402
Cw: 0701 1504
B: 5101 1517
HLA DRB1: 1101
HLA DRB3: 202
HLA DQA1: 501
HLA DQB1: 301
HLA DPB1: 0201 0402Serological ProfileHLA A: 24
HLA A ief: 24.1
HLA B: 51 63
HLA B ief: 51 15.1
Bw4/bw6: 4
HLA Dw: 5
HLA DR: 11
HLA Dw2: 25
HLA DR52: 52
HLA DQ: 7
HLA DP: 2Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Italian gender male growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
BRISTOL 8
Кат. номер 85011436-CDNA-20UL 85011436-DNA-5UG 85011436-RNA-5UG 85011436-1VL Cell Line OriginHuman B lymphocyteCell Line DescriptionBristol 8 is an HLA-A2 positive B lymphoblastoid cell line.ApplicationHLA profilesCulture MediumRPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human blood growth mode Suspension karyotype Not specified morphology Lymphoblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BRL 3A
Кат. номер 85111503-DNA-5UG 85111503-1VL 85111503-RNA-5UG Cell Line OriginBuffalo Rat liverCell Line DescriptionOriginally isolated by primary cloning. Serum-free medium conditioned by the cells produces a family of polypeptides termed multiplication stimulating activity (MSA).ApplicationGrowth factor studiesCulture MediumCoon′s modified Ham′s F12 + 2mM Glutamine + 5% Foetal Bovine Serum (FBS) or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol + 5% Foetal Bovine Serum (FBS) + 2mM Glutamine.Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rat liver growth mode Adherent karyotype Not specified morphology Epithelial products MSA factors receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BRL-1
Кат. номер 94121649-1VL 94121649-RNA-5UG 94121649-DNA-5UG Cell Line OriginRat epithelial cellsCell Line DescriptionIsolated by Dr Hayden Coon. Flat epithelial cell colonies that appear upon low density plating as suspensions of collagenase dissociated buffalo rat liver. Cell origin not clearly defined.ApplicationFeeder cells for co-culture with freshly isolated hepatocytes.Culture MediumCoon′s modified Ham′s F12 + 2mM Glutamine + 5% Foetal Bovine Serum (FBS) or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol + 5% Foetal Bovine Serum (FBS) + 2mM Glutamine.Subculture RoutineSpit confluent cultures 1:100 i.e. seeding at 1x1,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rat liver growth mode Adherent karyotype Diploid or pseudodiploid morphology Epithelial products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BRO, LJ human cell line
Кат. номер 94082269-DNA-5UG 94082269-CDNA-20UL 94082269-RNA-5UG 94082269-1VL General descriptionHomozygous: No
Consanguineous: NoCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileHLA DRB1: 301
HLA DRB3: 101
HLA DQAP: 4.1
HLA DQA1: 501
HLA DQB1: 201
HLA DPB1: 01011 02012Serological ProfileHLA A: 2 29
HLA A ief: 2.2 29.2
HLA B: 44 21
HLA B ief:Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Caucasian gender not specified growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
BS1-B4
Кат. номер 85011410-1VL 85011410-DNA-5UG 85011410-RNA-5UG Cell Line OriginMouse 3T3 Swiss Albino embryo fibroblastCell Line DescriptionEmbryonic fibroblast line from a Swiss Albino mouse. A variant of 3T3 which lacks terminal galactose on cell surface glycoproteins.ApplicationMembrane studiesCulture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse embryo growth mode Adherent karyotype Not specified morphology Fibroblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BSC1
Кат. номер 85011422-1VL 85011422-DNA-5UG 85011422-RNA-5UG Cell Line OriginMonkey African green kidney epithelialCell Line DescriptionContinuous cell line which when infected with SV40 virus develops characteristic cytopathic effect.Culture MediumEMEM (EBSS), (M2279) + 2 mM L-Glutamine, (G7513) + 1% NEAA, (M7145) + 10% FBS / FCS, (F2442).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin trypsin/EDTA; 5% CO2; 37 °C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source African green monkey kidney growth mode Adherent karyotype 60 morphology Epithelial, Not specified products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
BSH 012 human cell line
Кат. номер 94082279-RNA-5UG 94082279-CDNA-20UL 94082279-1VL 94082279-DNA-5UG General descriptionHomozygous: No
Consanguineous: NoCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileA: 01 26
B: 08 38
HLA DRB1: 0301 0402
HLA DRB3: 101
HLA DRB4: 101
HLA DQAP: 3.1 4.1
HLA DQA1: 0501 03
HLA DQB1: 0201 0302
HLA DPB1: 01011 0401Serological ProfileHLA A: 1 26
HLA B: 8 38
HLA B ief: 38.1Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Not specified gender female growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену
Получите коммерческое предложение в течение 1 часа
Менеджер подготовит коммерческое предложение и позвонит, если понадобится уточнить детали вашего заказа
Анна Гловацкая
Менеджер по работе с клиентами
С 2010 года мы поставляем оборудование с заводов Европы. Берем на себя все — от подбора оборудования до внедрения на предприятии
Все сотрудники имеют высшее образование, закончили ведущие химические вузы страны, такие как РХТУ им Менделеева.
У большинства компаний срок ожидания составляет 10-12 недель.
Оборудование хранится на сухом отапливаемом складе, где поддерживается ровная температура.
Работаем с PonyExpress и Деловыми линиями. Вы также можете выбрать свою транспортную компанию или забрать товар со склада в Москве.
В случае любых неполадок за свой счет выполним ремонт в сервисном центре или на заводе-изготовителе. Или бесплатно заменим прибор на новый.
Производим пуско-наладку оборудования, валидацию, обучение сотрудников. Если нужно, привлекаем инженеров с заводов- изготовителей.
