Cell Lines & Specialty Cell Culture
- Сортировать:
- Вид таблицей
-
AZH human cell line
Кат. номер 94071439-RNA-5UG 94071439-DNA-5UG 94071439-1VL 94071439-CDNA-20UL General descriptionHomozygous: YesCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileHLA DRB1: 1601
HLA DRB5: 201
HLA DQA1: 102
HLA DQB1: 502
HLA DPB1: 301Serological ProfileHLA A: 24
HLA C: 1
HLA B: 56
Bw4/bw6: 6
HLA Dw: 21
HLA DR: 16
HLA DR51: 51
HLA DQ: 5Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Israeli gender not specified growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
b.End3
Кат. номер 96091929-RNA-5UG 96091929-DNA-5UG 96091929-1VL Cell Line OriginMouse SV129 brain endotheliomaCell Line DescriptionThe cell line b.End3 has been established from brain endothelial cells of SV129 mice. Immortalisation has been carried out by infection of primary cells with retrovirus coding for the Polyoma virus middle T-antigen. b.End3 cells are positive for endothelial specific proteins (PECAM-1, Endoglin, MECA-32, Flk-1) tested by FACS. Inflammatory cytokines induce the expression of proteins such as ICAM-1, VCAM-1 and E-selectin. Injection into mice causes development of haemangiomas.ApplicationStudy of endothelial cell growth, differentiation and influence of oncogenes.Culture MediumDMEM + 2mM Glutamine + 5μM 2-Mercaptoethanol (2ME) + 1mM Sodium Pyruvate (NaP) + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:4 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse brain availability available only in EU growth mode Adherent karyotype Not specified morphology Endothelial-like products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
b.End5
Кат. номер 96091930-DNA-5UG 96091930-RNA-5UG 96091930-1VL Cell Line OriginMouse Balb/c brain endotheliomaCell Line DescriptionThe cell line b.End5 has been established from brain endothelial cells of BALB/c mice. Immortalisation has been carried out by infection of primary cells with retrovirus coding for the Polyoma virus middle T-antigen. b.End5 cells are positive for endothelial specific proteins (PECAM-1, Endoglin, MECA-32, Flk-1) tested by FACS. Inflammatory cytokines induce the expression of proteins such as CAM-1, VCAM-1 and E-selectin. Injection into mice causes development of haemangiomas.ApplicationStudy of endothelial cell growth, differentiation and influence of oncogenes.Culture MediumDMEM + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:4 i.e. seeding at 4x10,000 cells/cm using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse brain growth mode Adherent karyotype Not specified morphology Endothelial-like products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
B12
Кат. номер 85042303-1VL 85042303-DNA-5UG 85042303-RNA-5UG Cell Line OriginRat nervous tissue glialCell Line DescriptionEthyl nitrosourea induced tumour cell line. Glial cells are useful in the research of the distribution of neurotransmitter synthesis and brain specific antigens among nerve and glia.ApplicationNeurobiological studiesCulture MediumDMEM + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rat nerve growth mode Adherent karyotype Not specified morphology Glial products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
B12.F8
Кат. номер 11060102-1VL 11060102-DNA-5UG 11060102-CDNA-20UL 11060102-RNA-5UG Cell Line OriginHuman anti-hepatitis C virus IgG1 secreting B cellCell Line DescriptionHuman B-lymphocyte cell line, immortalized with Epstein-Barr Virus (EBV), derived from a patient with chronic Hepatitis C (HCV type 1). Cell line produces human monoclonal antibody specific to the native HCV nucleoprotein N3. This antibody binds to native HCV nucleoprotein with no observable reactivity for the NS3, NS4 and NS5 regions. This antibody identifies a major B cell epitope within the immunodominant nucleoprotein amino terminal subregion (aa. 34-45). B12.F8 can be used in immunochemistry, HCV protein purification and HCV typing.CM3.B6 (Sigma product number 11060101) and B12.F8 were derived from the same patient as confirmed by STR profiling.DNA ProfileSTR-PCR Data:
Amelogenin: X
CSF1PO: 11,12
D13S317: 9,11
D16S539: 11,12
D5S818: 11,13
D7S820: 8,11
THO1: 6
TPOX: 9,11
vWA: 14,16Culture MediumRPMI 1640, (R0883) + 2 mM L-L-Glutamine, (G7513) + 1% NEAA, (M7145) + 1% NaP, (S8636) + 10% FBS / FCS, (F2442) + 20U/ml Interleukin-6 (IL-6).Subculture RoutineMaintain cultures between 3-9 x 105/ml. 5% CO2; 37 °C. Media change every four days. Saturation density 1.1 x 106/ml.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.Параметрыbiological source human blood growth mode Suspension karyotype Not specified morphology Small rounded cells products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
B16 melanoma 4A5 Cell Line from mouse
Кат. номер 94042254-DNA-5UG 94042254-RNA-5UG 94042254-1VL Cell Line OriginMouse melanoma, melanin producingCell Line DescriptionDerived from a melanoma from the skin of a C57BL/6 strain mouse, showing fibroblast-like characteristics, which produces melanin. Cells may lose ability to produce pigmentation in long term culture.ApplicationB16 melanoma 4A5 cell line has been used to study the spectral and biological characteristics of copper (II) mesoporphyrinic complexes. It has also been used to evaluate the cytotoxicity of JaZ-30, a matrix metalloproteinase-2 (MMP-2) inhibitor.Culture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse skin growth mode Adherent karyotype Not specified morphology Fibroblast-like products Melanin receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
B16-F0 Cell Line from mouse 92101204-1VL
Cell Line OriginMouse melanoma, producing melaninCell Line DescriptionMouse melanoma which produces melanin.Culture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply, for more information.Параметрыbiological source mouse skin Quality Level 100, description Mouse melanoma, producing melanin growth mode Adherent karyotype Not specified morphology Epithelial products Melanin receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
B16-F1 Cell Line from mouse
Кат. номер 92101203-DNA-5UG 92101203-RNA-5UG 92101203-1VL Cell Line OriginMouse melanoma, producing melaninCell Line DescriptionMouse melanoma which produces melanin.ApplicationB16-F1 cell line from mouse has been used to study the cytotoxic and antioxidant properties of supercritical CO2 extract (SCE) of old mans beard (Usnea barbata). It has also been used to study the antiproliferative activity of essential oils from Achillea ligustica.Culture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse skin description Mouse melanoma, producing melanin growth mode Adherent karyotype Not specified morphology Fibroblast-like products Melanin receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
B2.Sp
Кат. номер 90030906-1VL 90030906-RNA-5UG 90030906-DNA-5UG Cell Line OriginBovine spleenCell Line DescriptionDerived from a normal spleen of a new born cow (Bos taurus).Culture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source bovine spleen growth mode Adherent karyotype Not specified morphology Not specified products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
B3/25
Кат. номер 94050507-DNA-5UG 94050507-RNA-5UG 94050507-1VL Cell Line OriginMouse S194/5.XXO.BU.1 x Mouse spleen cellsCell Line DescriptionThe antibody is directed against an acidic glycoprotein (dimer of 2x110kDa monomers joined by disulphide bonds) present on immature and proliferating human haematopoietic cells but not circulating peripheral blood cells, or other non haematopoietic cells.ImmunogenK-562 cellsCulture MediumDMEM + 2mM glutamine + 0.02mM 2-mercaptoethanol (2ME) + 10% Horse Serum.Subculture RoutineMaintain cultures between 100,000 - 1,000,000 cells/ml; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon Mouse spleen Myeloma S194/5.XXO.BU.1 Reactivity Anti-Haematopoietic Cell, human application(s) cell culture | mammalian: suitable isotype IgG shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
B50
Кат. номер 85042302-DNA-5UG 85042302-RNA-5UG 85042302-1VL Cell Line OriginRat nervous tissue neuronalCell Line DescriptionEthyl nitrosourea induced tumour cell line. Neuronal cells are useful in the research of the distribution of neurotransmitter synthesis and brain specific antigens among nerve and glia.ApplicationNeurobiological studiesCulture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rat nerve growth mode Adherent karyotype Not specified morphology Neuronal products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
B65
Кат. номер 85042305-RNA-5UG 85042305-DNA-5UG 85042305-1VL Cell Line OriginRat nervous tissue neuronalCell Line DescriptionEthyl nitrosourea induced tumour cell line. Neuronal cells are useful for research into the distribution of neurotransmitter synthesis and brain specific antigens among nerve and glia. Cell morphology significantly affected when grown in serum free media.ApplicationNeurobiological studiesCulture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rat nerve growth mode Adherent karyotype Not specified morphology Neuronal products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
B82
Кат. номер 85011408-DNA-5UG 85011408-RNA-5UG 85011408-1VL Cell Line OriginMouse C3H/An sc areolar and adipose tissueCell Line DescriptionDerivative of NCTC clone 929 (Sigma Catalogue number. 88102702) obtained by progressive selection in BUdR, has a cloning efficiency of 70-80%.ApplicationChromosome analysis and hybridisation studiesCulture MediumEMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse adipose tissue growth mode Adherent karyotype 2n = 51 morphology Fibroblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
B82 (Hamprecht)
Кат. номер 8062522-RNA-5UG 8062522-DNA-5UG 8062522-1VL Cell Line OriginMouse Fibroblast (L cells)Cell Line DescriptionAn 8-bromodeoxyuridine resistant mutant of mouse L cell fibroblasts (Mouse strain C3H/An). B82 lacks thymidine kinase and was isolated by virtue of resistance to 8-bromodeoxyuridine (30 μg/ml). ECACC has two catalogue entries for the B82 cell line which differ in that they were deposited by different groups: B82 (Sigma Catalogue number 85011408) and B82(Hamprecht) (Sigma Catalogue number 08062522). Several other cell lines in the ECACC catalogue have been derived from B82(Hamprecht).ApplicationUsed in the generation of cell hybrids.Culture MediumDMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 1 mM 8-bromodeoxyuridineSubculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse connective tissue growth mode Adherent karyotype Modal No of chromosomes is 53 morphology Fibroblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
B9 Cell Line mouse 12121201-1VL
Cell Line OriginMouse B cell hybridoma, IL-6 assayCell Line DescriptionB9 is a mouse B cell hybridoma line that requires Interleukin 6 (IL-6) for survival and proliferation. IL-6 is a pluripotent cytokine with multiple effects on many different cell types. It is produced by a variety of cells in response to various stimuli. The B9 hybridoma allows for a simple sensitive assay for human, rat, rabbit, pig and mouse IL-6 based on the IL-6 dependent proliferation of this murine B cell line. Details of the B9 hybridoma growth factor assay can be found in Current protocols in Immunology 1996 Cytokines and Their Cellular receptors 6.6.1-6.6.5 contributed by Nordan RP, Richards CD and Gauldie J. John Wiley & Sons, Inc.Culture MediumIDMEM or RPMI 1640 + 2mM Glutamine + 50μM 2-Mercaptoethanol (2ME) + 50pg/ml recombinant human IL-6 (Sigma SRP3096, diluted and stored in PBS containing 1% BSA) + 5% FBS. IL-6 needs to be added fresh at each media change.Subculture RoutineFor B9 cell maintenance cells should be seeded at 0.5 x 105 cells/ml in B9 culture medium. The culture will be in log phase growth between days 3-5. When the culture reaches 1 x 106 cells/ml (4-5 days) the culture must be sub cultured as above again seeding at 0.5 x 105 cells/ml.; 5% CO2; 37°C. It maybe necessary to determine the activity of the IL-6 or IL-6 containing supernatant first. The lowest concentration at which maximal growth can be obtained should be used. A fresh vial of cells is required when the background response from control cultures begins to rise. It is important that the culture conditions are adhered to avoid the B9 cells becoming IL-6 independent.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.ПараметрыQuality Level 100, biological source mouse spleen description Mouse B cell hybridoma, IL-6 assay growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
B92
Кат. номер 85042304-DNA-5UG 85042304-RNA-5UG 85042304-1VL Cell Line OriginRat nervous tissue glialCell Line DescriptionEthyl nitrosaurea induced tumour cell line. Neuronal and glial cells are useful for research into the distribution of neurotransmitter synthesis and brain specific antigens among nerve and glia. Morphology significantly affected when grown in serum free media.ApplicationNeurobiological studiesCulture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rat nerve growth mode Adherent karyotype Not specified morphology Glial/neuronal products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BAE-1
Кат. номер 88031149-RNA-5UG 88031149-DNA-5UG 88031149-1VL Cell Line OriginBovine aortic endotheliumCell Line DescriptionDerived from the aorta of a Friesian cow. Cells form "sprouts" after confluence. Infected with Bovine Viral Diarrhoea Virus (BVDV) which is a common contaminant of ruminants and is non-hazardous to humans. Secretes proteoglycans and collagen.Culture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C. There is a tendancy for the cells to grow fibroblast-like until they are confluent. Once confluent the endothelial morphology prevailOther NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source bovine cardiovascular growth mode Adherent karyotype 59 or 60 morphology Endothelial products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C Узнать цену -
BAI, JE human cell line
Кат. номер 94082267-1VL 94082267-CDNA-20UL 94082267-RNA-5UG 94082267-DNA-5UG General descriptionHomozygous: No
Consanguineous: NoCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileB: 4402
HLA DRB1: 401
HLA DRB4: 101
HLA DQAP: 3.1
HLA DQA1: 3
HLA DQB1: 0301 0302
HLA DPB1: 0301 0401Serological ProfileHLA A: 1 2
HLA A ief: 2.2
HLA B: 8 44
HLA B ief:Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Caucasian gender not specified growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
BAI, NJ human cell line
Кат. номер 94082268-1VL 94082268-DNA-5UG 94082268-CDNA-20UL 94082268-RNA-5UG General descriptionHomozygous: No
Consanguineous: NoCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileHLA DRB1: 401
HLA DRB4: 101
HLA DQAP: 3.1
HLA DQA1: 3
HLA DQB1: 302
HLA DPB1: 0301 0401Serological ProfileHLA A: 1 2
HLA A ief: 2.2
HLA B: 8 62
HLA B ief: 62.2Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Caucasian gender not specified growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
BAOEC
Кат. номер 86123102-RNA-5UG 86123102-1VL 86123102-DNA-5UG Cell Line OriginBovine lung endotheliumCell Line DescriptionBAOEC is susceptible to bovine respiratory viruses and is infected with BVDV.ApplicationVirus studies: Bovine respiratory virusesCulture MediumDMEM or 199 + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source bovine lung growth mode Adherent karyotype Not specified morphology Endothelial products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BAT, J human cell line
Кат. номер 94082214-CDNA-20UL 94082214-RNA-5UG 94082214-1VL 94082214-DNA-5UG General descriptionHomozygous: No
Consanguineous: NoCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileCw: 501
HLA DRB1: 0404 1401
HLA DRB3: 202
HLA DRB4: 101
HLA DQAP: 1.3 3.1
HLA DQA1: 0101 03
HLA DQB1: 0302 0503
HLA DPB1: 01011 0301Serological ProfileHLA A: 2 31
HLA C: 3 5
HLA B: 44 60
Bw4/bw6: 4 6Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Not specified gender not specified growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
BB
Кат. номер 87101201-DNA-5UG 87101201-1VL 87101201-RNA-5UG Cell Line OriginFish Brown Bullhead posterior trunk tissueCell Line DescriptionDerived from the trunk tissue (excluding fins) of a 2 year old Brown Bullhead fish. These cells will tolerate a temperature range of 4-34°C. The optimal temperature range for cell proliferation is 25-30°C. BB is susceptible to infectious pancreatic necrosis virus and channel catfish virus.ApplicationVirus studies: Infectious pancreatic necrotis virus and Channel catfish virus and channel catfish virusCulture MediumEMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 using 0.25% trypsin/EDTA; 5% CO2; 23°C. Note: The cells do not grow well on glass. Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:3 and culture for aOther NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source fish trunk growth mode Adherent karyotype 2n = 60 morphology Fibroblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BBA4
Кат. номер 20912-DNA-5UG 20912-RNA-5UG 20912-1VL Cell Line OriginMouse x Mouse hybridomaCell Line DescriptionBBA4 was developed by immunizing mice with extracted T. brucei brucei cytoskeleton and fusing the mouse spleen cells with the myeloma Sp2-0/Ag.ImmunogenT. brucei brucei cytoskeleton (detergent extracted)ApplicationDetection of T.brucei basal bodiesCulture MediumDMEM (D6546), + 2mM L-Glutamine (G7513), + 10% FBS / FCS (F2442),Subculture RoutineMaintain cultures between 1-9 x 100,000 cells/ml; 5% CO2; 37°COther NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon Mouse spleen Myeloma Sp2-0/Ag Reactivity Anti-Trypanosoma brucei basal bodies application(s) cell culture | mammalian: suitable isotype IgM shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BC3H1
Кат. номер 86093001-RNA-5UG 86093001-DNA-5UG 86093001-1VL Cell Line OriginMouse brain tumourCell Line DescriptionEstablished in 1974 from a mouse brain neoplasm tumour induced by nitrosoethylurea, possess many properties characteristic of smooth muscle. Since then it has been suggested that BC3H1 is a skeletal line of ectodermal origin that is defective for commitment to terminal differentiation. The two distinct morphologies typical of this cell line are large spheroid cells and smooth-muscle-like cells. The fusiform is the more common.ApplicationAcetylcholine receptor studies.Culture MediumDMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).Subculture RoutineSpilt confluent cultures 1:3 to 1:10 i.e. seeding at 1-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse brain growth mode Adherent karyotype Not specified morphology Fibroblast products Acetylcholine receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BC9
Кат. номер 95030301-RNA-5UG 95030301-1VL 95030301-DNA-5UG Cell Line OriginMouse P3X63Ag8.653 x Mouse BALB/c spleenCell Line DescriptionThe monoclonal antibody BC9 detects bovine tumour necrosis factor (TNF) in a radioimmunoassay with a detection limit of 24 pg/ml. Bovine recombinant TNF can be neutralised in vitro by addition of BC9. Cytotoxic assays showed a neutralising effect using L929 and WEHI 164 clone 13 cells.ImmunogenBovine recombinant TNF-alphaApplicationDetection of TNF in bioassay or radioimmunoassay, in vivo studies (neutralisation of TNF).Culture MediumRPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)Subculture RoutineMaintain cultures between 5-6x100,000 cells/ml; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon BALB/c mouse spleen Myeloma P3X63Ag8.653 Reactivity Anti-TNF, bovine application(s) cell culture | mammalian: suitable isotype IgG1 shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BCK human cell line
Кат. номер 94050326-1VL 94050326-CDNA-20UL 94050326-RNA-5UG 94050326-DNA-5UG General descriptionHomozygous: No
Consanguineous: NoCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileA: 0201 3201
Cw: 0602 0304
B: 2708 1501
HLA DRB1: 15 11
HLA DQB1: 05 03Serological ProfileHLA A: 2 32
HLA C: 6 10
HLA B: 2708 62
Bw4/bw6: 6Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Welsh/Greek gender female growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
BCL1 Clone CW13.20-3B3
Кат. номер 90061904-1VL 90061904-DNA-5UG 90061904-RNA-5UG Cell Line OriginMouse BALB/c B cell leukaemiaCell Line DescriptionDerived from BCL1 by limiting dilution and does not spontaneously secrete IgM when cultured in pre-tested batches of FBS. The line is useful for studying soluble factors involved in B-cell differentiation.ApplicationB-cell differentiation studiesCulture MediumRPMI 1640 + 2mM Glutamine + 1% Sodium Pyruvate (NaP) + 1% Non Essential Amino Acids (NEAA) + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Cells attach lightly to surface which should be tapped sharply to dislodge the cells. Do not allow cells to become confluent, subculture at 75% to 90% confluency; free-floating aggregates accOther NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse blood growth mode Suspension karyotype Not specified morphology Lymphoblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BE(2)-C
Кат. номер 95011817-CDNA-20UL 95011817-RNA-5UG 95011817-1VL 95011817-DNA-5UG Cell Line OriginHuman Caucasian neuroblastomaCell Line DescriptionBE(2)-C is a clonal sub-line of SK-N-BE(2) (ECCAC catalogue no. 95011815) which was isolated from bone marrow of a 22-month-old male with disseminated neuroblastoma in 1972. They are reported to be multipotential with regard to neuronal enzyme expression and display a high capacity to convert tyrosine to dopamine. The cells show a small, refractile morphology with short, neurite-like cell processes and tend to grow in aggregates.The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.ApplicationChemotherapy development, chromosome studies, drug resistance, differentiation studies.DNA ProfileSTR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 9,11
D5S818: 12
D7S820: 9,10
THO1: 6
TPOX: 11
vWA: 18Culture MediumEMEM (EBSS) + 1% Non Essential Amino Acids (NEAA) : Ham′s F12 (1:1) + 2mM Glutamine + 15% Foetal Bovine Serum (FBS) (Heat Inactivated).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:50 to 1:100 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human nerve growth mode Adherent karyotype Modal no. 44 morphology Neuroblast-like products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) metastasis shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BE(2)-M17
Кат. номер 95011816-RNA-5UG 95011816-CDNA-20UL 95011816-DNA-5UG 95011816-1VL Cell Line OriginHuman Caucasian neuroblastomaCell Line DescriptionThe cell line BE(2)-M17 was isolated from SK-N-BE(2) (Sigma Catalogue number. 95011815) by limiting dilution. The cells show a population doubling time of 20-24 hours, grow in multi-layers but start to aggregate and float as the culture ages. The neuroblastic cells express properties of neuroadrenergic neurones and multiple, moderately long cell processes. BE(2)-M17 showed tyrosine hydroxylase and dopamine--hydroxylase activity and specific uptake of norepinephrine (noradrenalin). Expression of intermediate filaments was detected as well as neuron-specific enolase and moderate expression of vimentin. BE(2)-M17 are tumorigenic in nude mice.ApplicationStudy of neuroblastomaCulture MediumEMEM (EBSS):Ham′s F12 (1:1) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS) (Heat Inactivated).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:8 to 1:20 i.e. seeding at 4x10,000 cells/cm2 using 0.1% trypsin or PBS only; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human nerve growth mode Adherent karyotype Not specified morphology Fusiform products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BE10-7
Кат. номер 93040125-DNA-5UG 93040125-RNA-5UG 93040125-1VL Cell Line OriginRat, BDIX, brain, pre-malignantCell Line DescriptionClone of the line BE10-Intermediate (Sigma Catalogue number. 93040119). The cells have elevated levels of tissue plasminogen activity but do not grow in agar or in BDIX rats. The cells should not be used beyond passage 24 as they start to form tumours.ApplicationStudy of carcinogenesisCulture MediumDMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 1-2x100,000 cells/25cm2 using 0.25% trypsin/EDTA; 10-15% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rat brain growth mode Adherent karyotype Not specified morphology Not specified products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BE10-Intermediate
Кат. номер 93040119-1VL 93040119-DNA-5UG 93040119-RNA-5UG Cell Line OriginRat, BDIX, foetal brain, pre-malignantCell Line DescriptionProduced by passaging the latent period cell line BE10-Early (Sigma Catalogue number. 93040118) beyond passage 17. The cells possess elevated levels of tissue plasminogen activator activity, are not tumourigenic in BDIX rats and do not form colonies in agar. Do not be use beyond passage 40 as cell properties alter. The BE10 culture was originally derived 2 days after a single dose of ethylnitrosourea to pregnant rats. The control culture BE11 was derived 2 days after exposure to buffer.ApplicationStudy of changes in the latent period of carcinogenesisCulture MediumDMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x100,000 cells/cm2 using 0.25% trypsin/EDTA; 10-15% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rat brain growth mode Adherent karyotype Not specified morphology Not specified products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BE10-Late
Кат. номер 93040120-RNA-5UG 93040120-DNA-5UG 93040120-1VL Cell Line OriginRat, BDIX, foetal brain, malignantCell Line DescriptionMalignant cells produced on passaging BE10-Intermediate (Sigma Catalogue number. 93040119) beyond passage 40. The cells possess tissue plasminogen activator activity, are tumourigenic in BDIX rats and form colonies in agar.ApplicationStudy of carcinogenesis, tumourigenicity studiesCulture MediumDMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 1-2x100,000 cells/25cm2 using 0.25% trypsin/EDTA; 10-15% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rat brain growth mode Adherent karyotype Not specified morphology Not specified products Plasminogen activator activity receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BE11 (Early)
Кат. номер 93040121-RNA-5UG 93040121-1VL 93040121-DNA-5UG Cell Line OriginRat, BDIX, foetal brainCell Line DescriptionThe BE11 culture was derived 2 days after a single injection of buffer into pregnant rats. The cells do not form colonies in agar and are not tumourigenic in BDIX rats. Plasminogen activator activity is barely detectable. They are suitable controls for BE10 (Early) cells (93040118).ApplicationStudy of carcinogenesisCulture MediumDMEM + 2mM Glutamine + 15-20% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x100,000 cells/cm2 using 0.25% trypsin/EDTA; 10-15% CO2; 37°C. Cultures should reach 2x1,000,000 cells per 25cm2 flask. Subculture weekly.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rat brain growth mode Adherent karyotype Not specified morphology Epithelial products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BEA, PL human cell line
Кат. номер 94082237-RNA-5UG 94082237-1VL 94082237-DNA-5UG 94082237-CDNA-20UL General descriptionHomozygous: No
Consanguineous: NoCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileB: 4403
HLA DRB1: 0401 07
HLA DRB4: 101
HLA DQAP: 2.1 3.1
HLA DQA1: 03 0201
HLA DQB1: 0302 0201
HLA DPB1: 0401 1001Serological ProfileHLA A: 2 29
HLA C: 3
HLA B: 44 62
Bw4/bw6: 4 6Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Not specified gender not specified growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
BEAS-2B Cell Line human 95102433-1VL
Cell Line OriginHuman bronchial epithelium, normalCell Line DescriptionBEAS-2B cells were derived from normal bronchial epithelium obtained from autopsy of non-cancerous individuals. Cells were infected with a replication-defective SV40/adenovirus 12 hybrid and cloned. Squamous differentiation can be observed in response to serum. This ability can be used for screening chemical and biological agents inducing or affecting differentiation and/or carcinogenesis. The cell line has been applied for studies of pneumococcal infection mechanisms. BEAS-2B was described to express keratins and SV40 T antigen. Subculturing the cells before confluency is necessary as confluent cultures rapidly undergo squamous terminal differentiation.ApplicationBEAS-2B Cell line has been used to study differentiation of squamous cells and effect of biological and chemical agents on differentiation.DNA ProfileSTR-PCR Data: Amelogenin: X,Y
CSF1PO: 9,12
D13S317: 13
D16S539: 12
D5S818: 12,13
D7S820: 10,13
THO1: 7,9.3
TPOX: 6,11
vWA: 17,18Culture MediumBEGM, also known as LHC-9 with modification (available from Clonetics Corporation, CC3171 (BEBM) plus additives CC4175 or BEGM Bullet Kit, CC3170); Note: Additives supplied contain gentamycin which has been omitted for culture at ECACC; media is serum-free.Subculture RoutinePassage sub-confluent cultures using 0.25% trypsin or trypsin/EDTA. Incubate at room temperature for 5-10 min until cells detach. Add fresh medium and disperse cells, centrifuge and resuspend pellet in medium. Seed into new flasks at 1500 to 3000 cells per cm2. Flasks have to be precoated with a mixture of 0.01 mg/ml fibronectin, 0.03 mg/ml collagen and 0.001 mg/ml bovine serum albumin dissolved in BEGM. Add mixture at ratio of 0.2 ml per cm2 surface area. Incubate at 5% CO2, 37°C for at least 6 hours at 37oC. Remove excess fluid and allow flasks to dry by incubating at 37oC overnight, leaving the caps loose. Prior to addition of cells wash flask three times with PBS. Note: BEGM medium is almost serum-free, therefore trypsin inhibitor is essential. Add an equal or greater volume of trypsin inhibitor as trypsin used before, centrifuge and reseed cells in collagen coated flasks using culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line.
Please view the Terms & Conditions of Supply, for more information.ПараметрыQuality Level 100, biological source human lung description Human bronchial epithelium, normal growth mode Adherent karyotype Not specified morphology Epithelial-like products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
BEI human cell line
Кат. номер 86072902-1VL 86072902-RNA-5UG 86072902-CDNA-20UL General descriptionHomozygous: YesCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileHLA DQA1: 201
HLA DPB1: 0301 0401Serological ProfileHLA A: 2
HLA C: 6
HLA B: 57
Bw4/bw6: 4
HLA Dw: 11
HLA DR: 7
HLA DQ: 9
HLA DP: 4 5Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Not specified gender not specified growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
BEL6KG human cell line
Кат. номер 94122028-CDNA-20UL 94122028-RNA-5UG 94122028-DNA-5UG 94122028-1VL General descriptionHomozygous: No
Consanguineous: NoCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileA: 02
B: 4901 44
HLA DRB1: 1107 04
HLA DRB3: 202
HLA DQB1: 03Serological ProfileHLA A: 2
HLA C: 7 5
HLA B: 49 44
HLA DR: 11/3 4
HLA DQ: 7Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Caucasian gender female growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
BEL7MON human cell line
Кат. номер 94122029-DNA-5UG 94122029-1VL 94122029-RNA-5UG 94122029-CDNA-20UL General descriptionHomozygous: No
Consanguineous: NoCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileA: 02
B: 51 44
HLA DRB1: 1109 04
HLA DQA1: 03011 0501
HLA DQB1: 301Serological ProfileHLA A: 2
HLA C: 7
HLA B: 51 44
HLA DR: 4 11
HLA DQ: 7Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Caucasian gender female growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
BEL8-CC human cell line
Кат. номер 94122030-CDNA-20UL 94122030-RNA-5UG 94122030-1VL 94122030-DNA-5UG General descriptionHomozygous: No
Consanguineous: NoCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileA: 02
B: 07 44
HLA DRB1: 0101 0103
HLA DQA1: 101
HLA DQB1: 501
HLA DPB1: 0201 20011Serological ProfileHLA A: 2
HLA C: 5 7
HLA B: 7 44
HLA DR: 1 103
HLA DQ: 5Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Caucasian gender female growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
BER human cell line
Кат. номер 8805209-CDNA-20UL 8805209-1VL 8805209-RNA-5UG 8805209-DNA-5UG General descriptionConsanguineous: NoCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileA: 201
Cw: 602
B: 1302
HLA DRA: 101
HLA DRB1: 701
HLA DRB4: 101
HLA DQAP: 2.1
HLA DQA1: 201
HLA DQB1: 201
HLA DPA1: 1
HLA DPB1: 02012 0401Serological ProfileHLA A: 2
HLA A ief: 2.2
HLA C: 6
HLA B: 13
HLA B ief: 13.2
Bw4/bw6: 4
HLA Dw: 7
HLA DR: 7
HLA DR53: 53
HLA DQ: 2
HLA DP: 2 4Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.ПараметрыQuality Level 100, biological source human blood ethnicity German gender male growth mode Suspension karyotype Not specified morphology Lymphoblastoid shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
BeWo Cell Line human
Кат. номер 86082803-CDNA-20UL 86082803-RNA-5UG 86082803-1VL Cell Line OriginHuman choriocarcinomaCell Line DescriptionThe first human, trophoblastic endocrine cell type to be maintained in continuous culture. It was initiated from a malignant gestational choriocarcinoma of the foetal placenta. The cells secrete placental hormones including chorionic gonadotrophin (hCG), polypeptide hormones, lactogen, estrogenic and progestational steroids, estrone, estradiol, estriol and progesterone. A high rate of hCG Synthesis has been shown. Cellular differentiation of this stem cell type occurs when hormonal precursors are added to the medium. Due to the high glycolytic activity the cells have to be sub-cultured or supplied with fresh media every 24-48 hours to prevent glucose exhaustion. BeWo has the same DNA profile as JEG3 (Sigma catalogue number 92120308); JEG3 was established by serial cloning of BeWo.ApplicationHormone studies
Human BeWo cell line has been used to study the effects of Δ-9-tetrahydrocannabinol (Δ9-THC) on the proliferation and gene transcription in the BeWo trophoblast cell line. It has also been used to study placental transfer of highly purified non-dioxin-like polychlorinated biphenyls in BeWo cells.DNA ProfileSTR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 9,11
D16S539: 13,14
D5S818: 10,11
D7S820: 10,12
THO1: 9,9.3
TPOX: 8
vWA: 16Culture MediumHam′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C; medium change or subculture every 24-48 hours necessary.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human placenta description Human choriocarcinoma growth mode Adherent karyotype modal number =86 range 71-178 morphology Epithelial products Gonadotrophin, lactogen and steroid hormones receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
BF-11
Кат. номер 95050413-RNA-5UG 95050413-DNA-5UG 95050413-1VL Cell Line OriginRat ACI spleen x Mouse Sp2/0 Ag-14Cell Line DescriptionThe BF-11 hybridoma cell line secretes a rat monoclonal antibody named Epoclonalan that reacts with human erythropoietin. Spleen cells from ACI strain rats that had been immunised with human urinary erythropoietin have been fused with Sp2/0 Ag-14 to produce this cell line. The α, β and asialo forms of human erythropoietin are bound by the antibody to the same extent.ImmunogenHuman erythropoietinApplicationThe isotype of the monoclonal antibody produced by this cell line is rat IgG2a.
Immunological studies, isolation of erythropoietin by immuno-affinity methods.Culture MediumDMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)Subculture RoutineMaintain cultures between 100,000 - 1,000,000 cells/ml; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Rat x Mouse Hybridoma ImmunoDon Rat AC1 spleen cells Myeloma Sp2/0 Ag-14 Reactivity Anti-Erythropoietin, human application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BF-2
Кат. номер 21713-RNA-5UG 21713-1VL 21713-DNA-5UG Cell Line OriginFish bluegill fryCell Line DescriptionThe cell line BF-2 has been derived from a trypsinised suspension of pooled caudal portions of the trunk of 1 year old fingerlings (Bluegill fry, Lepomis macrochirus). Cells have been reported to be susceptible to a variety of fish viruses including viral haemorrhagic septicaemia virus (VHSV), infectious hematopoietic necrosis virus (IHNV), spring viraemia of carp virus (SVCV) and pike fry rhabdovirus (PFRV).ApplicationFish virus studiesCulture MediumEMEM(HBSS) (M5775), or EMEM (EBSS) (M2279), + 2mM L-Glutamine (G7513), + 850 mg/L Sodium Bicarbonate(NaHCO3) (S8761), + 1% Non Essential Amino Acids(NEAA) (M7145), + 120 mg/l Sodium Pyruvate(NaP) (S8636), + 10% FBS / FCS (F2442),; a mixture of MEM (HBSS) and MEM (EBSS) with 1-2% FBS is best for stability of monolayer and maintenance mediumSubculture RoutineSplit sub-confluent cultures (70-80%) 1:4 to 1:6 using 0.25% trypsin or trypsin/EDTA; cells replicate at 15 - 33°C, optimal temperature is 23 - 26°C; monolayer is stable for at least 10 days in maintenance medium (at 15 or 16°C for about 21 days). Fish cOther NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source fish caudal trunk growth mode Adherent karyotype Not specified morphology Fibroblast-like products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BFA
Кат. номер 87022601-DNA-5UG 87022601-1VL 87022601-RNA-5UG Cell Line OriginBovine aorta endothelium foetalCell Line DescriptionDerived from a bovine foetus.ApplicationVirus studiesCulture MediumHam′s F12 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source bovine cardiovascular growth mode Adherent karyotype Not specified morphology Endothelial products Collagen type 3 receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
BGE human cell line
Кат. номер 85121708-DNA-5UG 85121708-CDNA-20UL 85121708-RNA-5UG 85121708-1VL General descriptionHomozygous: No
Consanguineous: NoCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileA: 01 02
B: 52
HLA DRB1: 1502
HLA DRB5: 102
HLA DQB1: 6011Serological ProfileHLA A: 1 2
HLA B: 52
Bw4/bw6: 4
HLA Dw: 12
HLA DR: 2
HLA DQ: 1
HLA DP: 4Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Not specified gender not specified growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
BGM Cell Line
Кат. номер 90092601-1VL 90092601-DNA-5UG 90092601-RNA-5UG Cell Line OriginMonkey African green kidneyCell Line DescriptionUsed for the isolation of water borne viruses e.g. polio 1, 2, 3, Echo 3, 6, 7, 9, 12, 27, Coxsackie A9 + B1, 2, 3 and Reo 1.ApplicationVirus studies: polio, Echo, Coxsackie and Reo virusesCulture MediumEMEM (EBSS), (M2279) + 2 mM L-Glutamine, (G7513) + 1% NEAA, (M7145) + 10% FBS / FCS, (F2442).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:8 to 1:20, i.e., seeding at 5x1,000 to 2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source African green monkey kidney growth mode Adherent karyotype Not specified morphology Epithelial-like products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
BHK 21 (Clone 13) from hamster
Кат. номер 85011433-RNA-5UG 85011433-1VL Cell Line OriginHamster Syrian kidneyCell Line DescriptionSubclone of parent line derived from 5 1-day-old unsexed hamster kidneys. Used extensively for virus replication studies i.e. poliovirus, rabies, foot and mouth disease, VSV (Indiana strain), herpes simplex, Ad25 and arboviruses.ApplicationVirus replication studies
BHK 21 (Clone 13) from hamster has been used in viral transfection studies.Culture MediumGMEM + 2mM Glutamine + 5% Tryptose Phosphate Broth + 5-10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source hamster kidney growth mode Adherent karyotype 2n = 44, pseudodiploid morphology Fibroblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
BHK 21 STRAIN 31
Кат. номер 93120840-DNA-5UG 93120840-RNA-5UG 93120840-1VL Cell Line OriginHamster Syrian kidneyCell Line DescriptionUsed extensively for the production of foot and mouth disease virus.ApplicationVirus studiesCulture MediumGMEM + 2mM Glutamine + 10% Tryptose Phosphate Broth + 5% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source hamster kidney growth mode Adherent karyotype Not specified morphology Epithelial products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену
Получите коммерческое предложение в течение 1 часа
Менеджер подготовит коммерческое предложение и позвонит, если понадобится уточнить детали вашего заказа
Анна Гловацкая
Менеджер по работе с клиентами
С 2010 года мы поставляем оборудование с заводов Европы. Берем на себя все — от подбора оборудования до внедрения на предприятии
Все сотрудники имеют высшее образование, закончили ведущие химические вузы страны, такие как РХТУ им Менделеева.
У большинства компаний срок ожидания составляет 10-12 недель.
Оборудование хранится на сухом отапливаемом складе, где поддерживается ровная температура.
Работаем с PonyExpress и Деловыми линиями. Вы также можете выбрать свою транспортную компанию или забрать товар со склада в Москве.
В случае любых неполадок за свой счет выполним ремонт в сервисном центре или на заводе-изготовителе. Или бесплатно заменим прибор на новый.
Производим пуско-наладку оборудования, валидацию, обучение сотрудников. Если нужно, привлекаем инженеров с заводов- изготовителей.
