Cell Lines & Specialty Cell Culture
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UNEWi022-A 66540177-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary Cell Type: Fibroblast of dermis
Developmental Stage: Adult
Passage Number: P4
Reprogramming Method: Sendai Virus (KLF4, MYC, POU5F1, SOX2)
Other: From the same donor as UNEWi022-B, UNEWi022-C
Characterization
Marker Expression: NANOG(+), TRA160(+), SSEA4(+), SSEA1(-)
Differentiation Potential: Ectoderm (TUBB3+), Endoderm (AFP+), Mesoderm (ACTA2+)
Microbiology / Virus Screening
HIV1: Negative
HIV2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Microbial Growth: None Detected
Viability: Viable Post-Cryopreservation
Genotyping
Karyotype: P14, Chromosome 12 gain by molecular karyotype SNP array
Genotype: 16 allele STR profile available upon requestLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel®/Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (85-89) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) age-related macular degeneration shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi022-B 66540178-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Developmental stage: Adult
Passage number reprogrammed: P4
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list: KLF4,MYC,POU5F1,SOX2
Other: UNEWi022-A, UNEWi022-C
Characterization
Marker expression:
SSEA1(-),NANOG (+),SSEA-4 (+),TRA 1-60 (+)
Differentiation potency: Ectoderm TUBB3(+),Endoderm AFP (+),Mesoderm ACTA2 (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P14
Cell line karyotype: chromosome 12 gain
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (85-89) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) age-related macular degeneration shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi022-C 66540179-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Developmental stage: Adult
Passage number reprogrammed: P4
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list: KLF4,MYC,POU5F1,SOX2
Other:From the same donor: UNEWi022-A, UNEWi022-B
Characterization
Marker expression:
SSEA1(-),NANOG(+),TRA 1-60(+),SSEA-4(+)
Differentiation potency
Ectoderm: TUBB3(+)Endoderm: AFP (+)Mesoderm: ACTA2 (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P14
Cell line karyotype: No clinically significant imbalance was detected
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (85-89) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) age-related macular degeneration shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi023-A 66540441-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Passage number reprogrammed: P4
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Other: From the same donor: UNEWi023-B, UNEWi023-C
Characterization
Marker expression: TRA 1-60 (+),NANOG (+),SSEA-1 (-),SSEA-4 (+)
Differentiation potency: Ectoderm: TUJ-1 (+),Endoderm: Alpha FetoProtein(+),Mesoderm: SMA (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P9
Cell line karyotype: lack of X chromosome detected
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method Sendai virus description age (85-89) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) age-related macular degeneration shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi023-B 66540442-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Passage number reprogrammed: P4
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Other:From the same donor: UNEWi023-A, UNEWi023-C
Characterization
Marker expression: SSEA-4 (+),TRA 1-60 (+),SSEA-1 (-),NANOG (+)
Differentiation potency:Ectoderm: TUJ-1(+),Endoderm: Alpha FetoProtein (+),Mesoderm: SMA (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P9
Cell line karyotype: No clinically significant imbalance was detected
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (85-89) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) age-related macular degeneration shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi023-C 66540443-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Passage number reprogrammed: P4
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Other: From the same donor: UNEWi023-A, UNEWi023-B
Characterization
Marker expression:
NANOG (+) ,TRA 1-60 (+),SSEA-4(+),SSEA-1 (-)
Differentiation potency:Ectoderm: TUJ-1 (+),Endoderm: Alpha FetoProtein (+),Mesoderm: SMA (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P9
Cell line karyotype: No clinically significant imbalance was detected
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: mTeSR® 1
Passage Number banked (pre-EBiSC): P9
No. Vials banked (pre-EBiSC): 30Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (85-89) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) age-related macular degeneration shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi024-A 66540323-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Developmental stage: Adult
Passage number reprogrammed: P4
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list: KLF4,MYC,POU5F1,SOX2
Other:From the same donor: UNEWi024-B, UNEWi024-C
Characterization
Marker expression: TRA 1-60(+),SSEA-4 (+),SSEA-1 (-),NANOG (+)
Differentiation potency: Ectoderm: TUBB3 (+),Endoderm: AFP (+),Mesoderm: ACTA2 (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Cell line karyotype: No clinically significant imbalance was detected
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (75-79) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) age-related macular degeneration shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi024-B 66540324-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Developmental stage: Adult
Passage number reprogrammed: P4
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list: KLF4,MYC,POU5F1,SOX2
Other: From the same donor: UNEWi024-A, UNEWi024-C
Characterization
Marker expression: TRA 1-60 (+),SSEA-4 (+),NANOG (+),SSEA-1 (-)
Differentiation potency Ectoderm: TUBB3 (+),Endoderm: AFP (+),Mesoderm: ACTA2 (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P13
Cell line karyotype: No clinically significant imbalance was detected
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method Sendai virus description age (75-79) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) age-related macular degeneration shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi024-C 66540325-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Developmental stage: Adult
Passage number reprogrammed: P4
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4,MYC,POU5F1,SOX2
Other: From the same donor: UNEWi024-A, UNEWi024-B
Characterization
Marker expression: SSEA-4 (+),NANOG (+),TRA 1-60 (+),SSEA-1 (-)
Differentiation potency: Ectoderm: TUBB3 (+),Endoderm: AFP (+),Mesoderm: ACTA2 (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P13
Cell line karyotype: No clinically significant imbalance was detected
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method Sendai virus description age (75-79) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) age-related macular degeneration shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi025-A 66540328-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Passage number reprogrammed: P4
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list: KLF4,MYC,POU5F1,SOX2
Other: From the same donor: UNEWi025-B, UNEWi025-C
Characterization
Marker expression: NANOG (+),SSEA-4 (+),SSEA-1 (-),TRA 1-60 (+)
Differentiation potency: Ectoderm: TUBB3(+),Endoderm: AFP(+),Mesoderm: ACTA2 (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P12
Cell line karyotype: No clinically significant imbalance was detected
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method Sendai virus description age (70-74) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) age-related macular degeneration shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi025-B 66540329-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Passage number reprogrammed: P4
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Other:From the same donor: UNEWi025-A, UNEWi025-C
Characterization
Marker expression:
SSEA-1 (-),SSEA-4 (+),TRA 1-60 (+),NANOG (+)
Differentiation potency: Ectoderm: TUBB3(+),Endoderm: AFP(+),Mesoderm: ACTA2 (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P12
Cell line karyotype: No clinically significant imbalance was detected
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method Sendai virus description age (70-74) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) age-related macular degeneration shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi025-C 66540330-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Passage number reprogrammed: P4
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Other:From the same donor: UNEWi025-A, UNEWi025-B
Characterization
Marker expression: NANOG (+),SSEA-1 (-),SSEA-4 (+),TRA 1-60 (+)
Differentiation potency: Ectoderm: TUBB3 (+),Endoderm: AFP (+),Mesoderm: ACTA2 (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P12
Cell line karyotype: No clinically significant imbalance was detected
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method Sendai virus description age (70-74) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) age-related macular degeneration shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi026-A 66540444-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Passage number reprogrammed: P4
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Other:From the same donor: UNEWi026-B, UNEWi026-C
Characterization
Marker expression: SSEA-1(-),TRA 1-60 (+),NANOG (+),SSEA-4 (+)
Differentiation potency:Ectoderm: TUJ-1(+),Endoderm: Alpha FetoProtein (+),Mesoderm: SMA (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P10
Cell line karyotype: No clinically significant imbalance was detected
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method Sendai virus description age (60-64) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) diabetes (mellitus) shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi026-B 66540445-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Passage number reprogrammed: P4
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Other:From the same donor: UNEWi026-A, UNEWi026-C
Characterization
Marker expression: SSEA-1(-),NANOG (+),TRA 1-60 (+),SSEA-4 (+)
Differentiation potency:Ectoderm: TUJ-1 (+),Endoderm: Alpha FetoProtein(+),Mesoderm: SMA (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P11
Cell line karyotype: No clinically significant imbalance was detected
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (60-64) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) diabetes (mellitus) shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi026-C 66540446-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Passage number reprogrammed: P4
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Other:From the same donor: UNEWi026-A, UNEWi026-B
Characterization
Marker expression: NANOG (+),TRA 1-60 (+),SSEA-4 (+),SSEA-1 (-)
Differentiation potency:Ectoderm: TUJ-1 (+),Endoderm: Alpha FetoProtein(+),Mesoderm: SMA (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P12
Cell line karyotype: No clinically significant imbalance was detected
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (60-64) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) diabetes (mellitus) shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi027-A 66540406-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Passage number reprogrammed: P4
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Characterization
Analysis of Undifferentiated Cells
Marker expression: SSEA-4 (+)TRA 1-60 (+)SSEA-1 (-)NANOG (+)
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Marker Expressed:TUBB3 (+)
Endoderm: In vitro spontaneous differentiation
Marker Expressed:AFP (+)
Mesoderm: In vitro spontaneous differentiation
Marker Expressed:ACTA2 (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P30
Cell line karyotype: No clinically significant imbalance was detected
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (65-69) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) retinitis pigmentosa shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UOXFi001-A 66540384-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of OxfordCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Tissue collection date: May 3, 2011
Passage number reprogrammed: 4
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
NANOG
POU5F1
SOX2
Absence of reprogramming vector(s): yes
Have the reprogramming vectors been silenced: Unknown
Other:From the same donor: UOXFi001-B, UOXFi001-C, UOXFi001-D
Characterization
Analysis of Undifferentiated Cells
Marker expression: POU5F1 (OCT-4)(+)SSEA-4(+)TRA 1-60 (+)SSEA-1(+)
PluriTest: Pluripotency score: 24.23
Novelty score: 1.40
Microarray data: http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE53425
Report: MK071-1.PNG
Differentiation potency
Ectoderm: In vitro directed differentiation
Marker Expressed:HES5(+)NeuroD1 (+)Pax6(+)
Endoderm: In vitro directed differentiation
Marker Expressed:CXCR4(+)GATA6(+)Sox17(+)
Mesoderm: In vitro directed differentiation
Marker Expressed:MIXL1 (+)NCAM(+)Vimentin(+)
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Genotyping
Karyotyping
Passage number: 11
Cell line karyotype: 46, XX
Karyotyping method: Illumina Human CytoSNP-12v2.1 bead chip arrayLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: mTeSR® 1
Passage Number banked (pre-EBiSC): 8
No. Vials banked (pre-EBiSC): 30Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method retrovirus description age (70-74) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Parkinsons disease shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UOXFi001-B 66540385-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of OxfordCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Tissue collection date: May 3, 2011
Passage number reprogrammed: 4
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
NANOG
POU5F1
SOX2
Absence of reprogramming vector(s): yes
Have the reprogramming vectors been silenced: Unknown
Other:From the same donor: UOXFi001-A, UOXFi001-C, UOXFi001-D
Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-60(+)
PluriTest: Pluripotency score: 23.03
Novelty score: 1.37
Microarray data: http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE53425
Report: UOXFi001-B.PNG
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 11
Cell line karyotype: 46, XX
Karyotyping method: Illumina Human CytoSNP-12v2.1 bead chip array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method retrovirus description age (70-74) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Parkinsons disease shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UOXFi001-C 66540386-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of OxfordCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Tissue collection date: May 3, 2011
Passage number reprogrammed: 4
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
NANOG
POU5F1
SOX2
Absence of reprogramming vector(s): yes
Have the reprogramming vectors been silenced: Unknown
Other:From the same donor: UOXFi001-A, UOXFi001-B, UOXFi001-D
Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-60(+)
PluriTest: Pluripotency score: 23.29
Novelty score: 1.64
Report: UOXFi001-C.PNG
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Genotyping
Karyotyping
Passage number: 13
Cell line karyotype: 46, XX
Karyotyping method: Illumina Human CytoSNP-12v2.1 bead chip arrayLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: mTeSR® 1
Passage Number banked (pre-EBiSC): 9
No. Vials banked (pre-EBiSC): 30Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method retrovirus description age (70-74) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Parkinsons disease shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UOXFi001-D 66540387-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of OxfordCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Tissue collection date: May 3, 2011
Passage number reprogrammed: 4
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
NANOG
POU5F1
SOX2
Absence of reprogramming vector(s): yes
Have the reprogramming vectors been silenced: Unknown
Other:From the same donor: UOXFi001-A, UOXFi001-B, UOXFi001-C
Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-60(+)
PluriTest: Pluripotency score: 22.39
Novelty score: 1.44
Report: UOXFi001-D.PNG
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Genotyping
Karyotyping
Passage number: 13
Cell line karyotype: 46, XX
Karyotyping method: Illumina Human CytoSNP-12v2.1 bead chip arrayLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: mTeSR® 1
Passage Number banked (pre-EBiSC): 9
No. Vials banked (pre-EBiSC): 30Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method retrovirus description age (70-74) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Parkinsons disease shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UOXFi002-A 66540388-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of OxfordCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Tissue collection date: May 3, 2011
Passage number reprogrammed: 4
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
NANOG
POU5F1
SOX2
Absence of reprogramming vector(s): yes
Have the reprogramming vectors been silenced: Unknown
Other:From the same donor: UOXFi001-A, UOXFi001-B, UOXFi001-C
Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-60(+)
PluriTest: Pluripotency score: 22.39
Novelty score: 1.44
Report: UOXFi001-D.PNG
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Genotyping
Karyotyping
Passage number: 13
Cell line karyotype: 46, XX
Karyotyping method: Illumina Human CytoSNP-12v2.1 bead chip arrayLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: mTeSR® 1
Passage Number banked (pre-EBiSC): 9
No. Vials banked (pre-EBiSC): 30Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method retrovirus description age (50-54) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Parkinsons disease shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UOXFi002-B 66540389-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of OxfordCell Line DescriptionDerivation
Primary cell type: Fibroblast
Tissue collection date: June 21, 2011
Passage number reprogrammed: 6
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
NANOG
POU5F1
SOX2
Absence of reprogramming vector(s): yes
Have the reprogramming vectors been silenced: Unknown
Other:From the same donor: UOXFi002-A, UOXFi002-C
Characterization
Analysis of Undifferentiated Cells
Marker expression: NANOG(+)TRA 1-60(+)
PluriTest: Pluripotency score: 15.37
Novelty score: 1.46
Report: MK82-30.PNG
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 12
Cell line karyotype: 46, XY
Karyotyping method: Illumina Human CytoSNP-12v2.1 bead chip array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method retrovirus description age (50-54) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Parkinsons disease shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UOXFi002-C 66540390-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of OxfordCell Line DescriptionDerivation
Primary cell type: -
Reprogramming method
Vector type: None
Other:From the same donor: UOXFi002-A, UOXFi002-B
Characterization
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Genotyping
No Information availableLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineNo Information availableLegal InformationEBiSC is a trademark of Fraunhofer-GesellschaftПараметрыbiological source human dermis (fibroblast) reprogramming method retrovirus description age (50-54) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Parkinsons disease shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UOXFi003-A 66540391-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of OxfordCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Tissue collection date: July 6, 2011
Passage number reprogrammed: 5
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
NANOG
POU5F1
SOX2
Absence of reprogramming vector(s): yes
Have the reprogramming vectors been silenced: Unknown
Other:From the same donor: UOXFi003-C
Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-60(+)
hPSC Scorecard: Self-renewal,Endoderm,Mesoderm,Ectoderm
PluriTest: Pluripotency score: 23.43
Novelty score: 1.67
Microarray data: http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE53425
Report: UOXFi003-A.PNG
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 11
Cell line karyotype: 46, XY
Karyotyping method: Illumina Human CytoSNP-12v2.1 bead chip array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method retrovirus description age (45-49) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Parkinsons disease shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UOXFi003-C 66540392-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of OxfordCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Tissue collection date: July 6, 2011
Passage number reprogrammed: 4
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
NANOG
POU5F1
SOX2
Absence of reprogramming vector(s): yes
Have the reprogramming vectors been silenced: Unknown
Other:From the same donor: UOXFi003-A
Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-60(+)
PluriTest: Pluripotency score: 36.84
Novelty score: 1.48
Report: UOXFi003-C.PNG
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Genotyping
Karyotyping
Passage number: 12
Cell line karyotype: 46, XY
Karyotyping method: Illumina Human CytoSNP-12v2.1 bead chip arrayLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: mTeSR® 1
Passage Number banked (pre-EBiSC): 8
No. Vials banked (pre-EBiSC): 30Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method retrovirus description age (45-49) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Parkinsons disease shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UOXFi004-A 66540393-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of OxfordCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Tissue collection date: June 1, 2013
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
NANOG
POU5F1
SOX2
Absence of reprogramming vector(s): yes
Have the reprogramming vectors been silenced: Unknown
From the same donor: UOXFi004-B
Characterization
Analysis of Undifferentiated Cells
Marker expression: NANOG(+)TRA 1-60(+)
PluriTest: Pluripotency score: 31.38
Novelty score: 1.36
Report: OX1-18.PNG
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 26
Cell line karyotype: 46, XY
Karyotyping method: CytoSNP-12v2.1 bead chip arrayLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: mTeSR® 1
Passage Number banked (pre-EBiSC): 25Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method retrovirus description age (35-39) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UOXFi004-B 66540394-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of OxfordCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
NANOG
POU5F1
SOX2
Absence of reprogramming vector(s): yes
Have the reprogramming vectors been silenced: Unknown
Other:From the same donor: UOXFi004-A
Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-60(+)NANOG(+)
PluriTest: Pluripotency score: 30.06
Novelty score: 1.35
Report: OX1-19.PNG
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 22
Cell line karyotype: 46, XY
Karyotyping method: Illumina Human CytoSNP-12v2.1 bead chip arrayLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 21%
CO2 concentration: 5%
Culture medium: Essential E8®
Passage Number banked (pre-EBiSC): 22Legal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method retrovirus description age (35-39) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UOXFi005-A 66540395-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of OxfordCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Tissue collection date: April 4, 2011
Passage number reprogrammed: 5
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
NANOG
POU5F1
SOX2
Absence of reprogramming vector(s): yes
Have the reprogramming vectors been silenced: Unknown
From the same donor: UOXFi005-B, UOXFi005-C
Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-60(+)
PluriTest: Pluripotency score: 23.91
Novelty score: 1.4
Report: JR053-1.PNG
Differentiation potency
Ectoderm: In vitro spontaneous differentiation,In vitro directed differentiation
Marker Expressed:HES5 (+)NeuroD1 (+)PAX6(+)
Endoderm: In vitro spontaneous differentiation,In vitro directed differentiation
Marker Expressed:CXCR4 (+)GATA6 (+)SOX17(+)
Mesoderm: In vitro spontaneous differentiation,In vitro directed differentiation
Marker Expressed:MIXL1 (+)NCAM1 (+)VIM(+)
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 22
Cell line karyotype: 46, XY
Karyotyping method: Illumina Human OmniExpressv1.1/ bead chip array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method retrovirus description age (65-69) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UOXFi005-B 66540396-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of OxfordCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Tissue collection date: April 4, 2011
Passage number reprogrammed: 5
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
NANOG
POU5F1
SOX2
Absence of reprogramming vector(s): yes
Have the reprogramming vectors been silenced: Unknown
Other:From the same donor: UOXFi005-A, UOXFi005-C
Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-60(+)
PluriTest: Pluripotency score: 22.77
Novelty score: 1.48
Report: UOXFi005-B.PNG
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Genotyping
Karyotyping
Passage number: 20
Cell line karyotype: 46, XY
Karyotyping method: Illumina Human OmniExpressv1.1/ bead chip arrayLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: mTeSR® 1
Passage Number banked (pre-EBiSC): 10
No. Vials banked (pre-EBiSC): 30Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method retrovirus description age (65-69) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UOXFi005-C 66540397-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of OxfordCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Tissue collection date: April 4, 2011
Passage number reprogrammed: 5
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
NANOG
POU5F1
SOX2
Absence of reprogramming vector(s): yes
Have the reprogramming vectors been silenced: Unknown
Other:From the same donor: UOXFi005-A, UOXFi005-B
Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-60(+)
PluriTest: Pluripotency score: 24.81
Novelty score: 1.54
Report: UOXFi005-C.PNG
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Genotyping
Karyotyping
Passage number: 15
Cell line karyotype: 46, XY
Karyotyping method: Illumina Human CytoSNP-12v2.1 bead chip arrayLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: mTeSR® 1
Passage Number banked (pre-EBiSC): 9
No. Vials banked (pre-EBiSC): 30Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method retrovirus description age (65-69) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UOXFi006-A 66540398-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of OxfordCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Tissue collection date: Oct. 8, 2010
Passage number reprogrammed: 2
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
NANOG
POU5F1
SOX2
Absence of reprogramming vector(s): yes
Have the reprogramming vectors been silenced: Unknown
Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-60(+)NANOG(+)
PluriTest: Pluripotency score: 22.22
Novelty score: 1.75
Report: OX2-28.PNG
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 12
Cell line karyotype: 46, XY
Karyotyping method: CytoSNP-12v2.1 bead chip array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method retrovirus description age (40-44) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UOXFi009-A 66540548-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of OxfordCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Location of primary tissue procurement: Zone of skin
Tissue collection year: 2011
Passage number reprogrammed: 5
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
Nanog
POU5F1
SOX2
Have the reprogramming vectors been silenced: Yes
Merthods used: rtpcr
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-60 Yes -
NANOG Yes -
PluriTest: Pluripotency score: 27.18
Novelty score: 1.4
Report: Pluritest MK024-3.PNG
The PluriTest is a pluripotency assessment based on DNA microarray data, which considers the genome-wide expression profile of the sample (www.nature.com/nmeth/journal/v8/n4/full/nmeth.1580.html).
PluriTest scores should be assessed in the context of the empirical distribution of pluripotent and somatic samples used to construct and validate PluriTest (e.g. Exemplary use of PluriTest). The training set of iPSC clustered with pluripotency score > 25 and novelty score < 1.67 with 98% sensitivity and 100% specificity. Cell lines with pluritest scores > 10 also cluster with the PluriTest training data set with high sensitivy and specificity.
Morphology:
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 11
Cell line karyotype: 46 XY
Karyotyping method: Molecular karyotyping by SNP array
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 21%
CO2 concentration: 5%
Culture medium: mTeSR® 1
Passage Number banked (pre-EBiSC): 9
No. Vials banked (pre-EBiSC): 3Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method retrovirus description age (60-64) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Parkinsons disease shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UOXFi009-B 66540558-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of OxfordCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Location of primary tissue procurement: Zone of skin
Tissue collection year: 2011
Passage number reprogrammed: 5
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
NANOG
POU5F1
SOX2
Have the reprogramming vectors been silenced: Yes
Merthods used: rtpcr
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-60 Yes -
PluriTest: Pluripotency score: 25.7
Novelty score: 1.29
Report: Pluritest.PNG
The PluriTest is a pluripotency assessment based on DNA microarray data, which considers the genome-wide expression profile of the sample (www.nature.com/nmeth/journal/v8/n4/full/nmeth.1580.html).
PluriTest scores should be assessed in the context of the empirical distribution of pluripotent and somatic samples used to construct and validate PluriTest (e.g. Exemplary use of PluriTest). The training set of iPSC clustered with pluripotency score > 25 and novelty score < 1.67 with 98% sensitivity and 100% specificity. Cell lines with pluritest scores > 10 also cluster with the PluriTest training data set with high sensitivy and specificity.
Morphology:
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 11
Cell line karyotype: 46XY
Karyotyping method: Molecular karyotyping by SNP array
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: mTeSR® 1
Passage Number banked (pre-EBiSC): 14
No. Vials banked (pre-EBiSC): 3Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method retrovirus description age (60-64) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Parkinsons disease shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UOXFi009-C 66540559-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of OxfordCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Location of primary tissue procurement: Zone of skin
Tissue collection year: 2011
Passage number reprogrammed: 5
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
NANOG
POU5F1
SOX2
Have the reprogramming vectors been silenced: Yes
Merthods used: rtpcr
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-60 Yes -
PluriTest: Pluripotency score: 23.38
Novelty score: 1.38
Report: Pluritest.PNG
The PluriTest is a pluripotency assessment based on DNA microarray data, which considers the genome-wide expression profile of the sample (www.nature.com/nmeth/journal/v8/n4/full/nmeth.1580.html).
PluriTest scores should be assessed in the context of the empirical distribution of pluripotent and somatic samples used to construct and validate PluriTest (e.g. Exemplary use of PluriTest). The training set of iPSC clustered with pluripotency score > 25 and novelty score < 1.67 with 98% sensitivity and 100% specificity. Cell lines with pluritest scores > 10 also cluster with the PluriTest training data set with high sensitivy and specificity.
Morphology:
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 11
Cell line karyotype: 46 XX
Karyotyping method: Molecular karyotyping by SNP array
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: mTeSR® 1
Passage Number banked (pre-EBiSC): 11
No. Vials banked (pre-EBiSC): 3Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method retrovirus description age (60-64) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Parkinsons disease shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UOXFi010-A 66540560-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of OxfordCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Location of primary tissue procurement: Zone of skin
Tissue collection year: 2010
Passage number reprogrammed: 4
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
POU5F1
SOX2
Have the reprogramming vectors been silenced: Yes
Merthods used: rtpcr
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-60 Unknown -
PluriTest: Pluripotency score: 21.11
Novelty score: 1.55
Report: Pluritest.PNG
The PluriTest is a pluripotency assessment based on DNA microarray data, which considers the genome-wide expression profile of the sample (www.nature.com/nmeth/journal/v8/n4/full/nmeth.1580.html).
PluriTest scores should be assessed in the context of the empirical distribution of pluripotent and somatic samples used to construct and validate PluriTest (e.g. Exemplary use of PluriTest). The training set of iPSC clustered with pluripotency score > 25 and novelty score < 1.67 with 98% sensitivity and 100% specificity. Cell lines with pluritest scores > 10 also cluster with the PluriTest training data set with high sensitivy and specificity.
Morphology:
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 11
Cell line karyotype: 46XY
Karyotyping method: Molecular karyotyping by SNP array
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 21%
CO2 concentration: 5%
Culture medium: mTeSR® 1
Passage Number banked (pre-EBiSC): 11
No. Vials banked (pre-EBiSC): 3Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method retrovirus description age (55-59) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Parkinsons disease shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UOXFi010-B 66540553-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of OxfordCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Location of primary tissue procurement: Zone of skin
Tissue collection year: 2010
Passage number reprogrammed: 4
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
NANOG
POU5F1
SOX2
Absence of reprogramming vector(s): yes
Have the reprogramming vectors been silenced: Yes
Merthods used: rtpcr
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-60 Yes -
PluriTest: Pluripotency score: 34.33
Novelty score: 1.59
Report: JR023-2.PNG
The PluriTest is a pluripotency assessment based on DNA microarray data, which considers the genome-wide expression profile of the sample (www.nature.com/nmeth/journal/v8/n4/full/nmeth.1580.html).
PluriTest scores should be assessed in the context of the empirical distribution of pluripotent and somatic samples used to construct and validate PluriTest (e.g. Exemplary use of PluriTest). The training set of iPSC clustered with pluripotency score > 25 and novelty score < 1.67 with 98% sensitivity and 100% specificity. Cell lines with pluritest scores > 10 also cluster with the PluriTest training data set with high sensitivy and specificity.
Morphology:
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 11
Cell line karyotype: 46, XY
Karyotyping method: Molecular karyotyping by SNP array
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 21%
CO2 concentration: 5%
Culture medium: mTeSR® 1
Passage Number banked (pre-EBiSC): 4Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method retrovirus description age (55-59) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Parkinsons disease shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UOXFi010-C 66540554-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of OxfordCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Location of primary tissue procurement: Zone of skin
Tissue collection year: 2010
Passage number reprogrammed: 4
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
NANOG
POU5F1
SOX2
Have the reprogramming vectors been silenced: Yes
Merthods used: rtpcr
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-60 Unknown -
PluriTest: Pluripotency score: 21.12
Novelty score: 1.68
Report: Pluritest.PNG
The PluriTest is a pluripotency assessment based on DNA microarray data, which considers the genome-wide expression profile of the sample (www.nature.com/nmeth/journal/v8/n4/full/nmeth.1580.html).
PluriTest scores should be assessed in the context of the empirical distribution of pluripotent and somatic samples used to construct and validate PluriTest (e.g. Exemplary use of PluriTest). The training set of iPSC clustered with pluripotency score > 25 and novelty score < 1.67 with 98% sensitivity and 100% specificity. Cell lines with pluritest scores > 10 also cluster with the PluriTest training data set with high sensitivy and specificity.
Morphology:
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 10
Cell line karyotype: 46XY
Karyotyping method: Molecular karyotyping by SNP array
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 21%
CO2 concentration: 5%
Culture medium: mTeSR® 1
Passage Number banked (pre-EBiSC): 9
No. Vials banked (pre-EBiSC): 3Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method retrovirus description age (55-59) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Parkinsons disease shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UOXFi010-D 66540555-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of OxfordCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Location of primary tissue procurement: Zone of skin
Tissue collection year: 2010
Passage number reprogrammed: 4
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
NANOG
POU5F1
SOX2
Have the reprogramming vectors been silenced: Yes
Merthods used: rtpcr
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-60 Yes -
PluriTest: Pluripotency score: 27.23
Novelty score: 1.42
The PluriTest is a pluripotency assessment based on DNA microarray data, which considers the genome-wide expression profile of the sample (www.nature.com/nmeth/journal/v8/n4/full/nmeth.1580.html).
PluriTest scores should be assessed in the context of the empirical distribution of pluripotent and somatic samples used to construct and validate PluriTest (e.g. Exemplary use of PluriTest). The training set of iPSC clustered with pluripotency score > 25 and novelty score < 1.67 with 98% sensitivity and 100% specificity. Cell lines with pluritest scores > 10 also cluster with the PluriTest training data set with high sensitivy and specificity.
Morphology:
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 11
Cell line karyotype: 46 XY
Karyotyping method: Molecular karyotyping by SNP arrayLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 21%
CO2 concentration: 5%
Culture medium: mTeSR® 1
Passage Number banked (pre-EBiSC): 5
No. Vials banked (pre-EBiSC): 3Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method retrovirus description age (55-59) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Parkinsons disease shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
WTSIi002-A 66540072-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Wellcome Trust Sanger InstituteCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Location of primary tissue procurement: Arm
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Characterization
Analysis of Undifferentiated Cells
Marker expression: SOX2(+)POU5F1 (OCT-4)(+) NANOG (+)
PluriTest: Pluripotency score: 26.212
Novelty score: 1.356
Microarray data: http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Genotyping
Genome-wide analysis:
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ123009
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: Essential E8®
Passage method: EDTA
Matrix: Vitronectin
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-GesellschaftПараметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method Sendai virus description age (45-49) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
WTSIi003-A 66540073-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Wellcome Trust Sanger InstituteCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Location of primary tissue procurement: Arm
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Characterization
Analysis of Undifferentiated Cells
Marker expression: NANOG(+)POU5F1 (OCT-4)(+)SOX2(+)
PluriTest: Pluripotency score: 40.257
Novelty score: 1.464
Microarray data: http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Genotyping
Genome-wide analysis:
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ122989
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: Essential E8®
Passage method: EDTA
Matrix: Vitronectin
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-GesellschaftПараметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method Sendai virus description age (60-64) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
WTSIi004-A 66540074-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Wellcome Trust Sanger InstituteCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Location of primary tissue procurement: Arm
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: WTSIi004-B
Characterization
Analysis of Undifferentiated Cells
Marker expression: SOX2 (+)NANOG (+) POU5F1 (OCT-4) (+)
PluriTest: Pluripotency score: 58.365
Novelty score: 2.13
Microarray data: http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Genotyping
Genome-wide analysis:
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ123022
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: Essential E8®
Passage method: EDTA
Matrix: Vitronectin
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-GesellschaftПараметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method Sendai virus description age (35-39) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
WTSIi004-B 66540108-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Wellcome Trust Sanger InstituteCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Location of primary tissue procurement: Arm
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: WTSIi004-A
Characterization
Analysis of Undifferentiated Cells
Marker expression:SOX2(+)NANOG (+)POU5F1 (OCT-4)(+)
PluriTest: Pluripotency score: 39.653
Novelty score: 1.353
Microarray data: http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Genotyping
Genome-wide analysis:
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266800
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: Essential E8®
Passage method: EDTA
Matrix: Vitronectin
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-GesellschaftПараметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method Sendai virus description age (35-39) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
WTSIi005-A 66540075-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Wellcome Trust Sanger InstituteCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Location of primary tissue procurement: Arm
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: WTSIi005-B
Characterization
Analysis of Undifferentiated Cells
Marker expression: NANOG (+) POU5F1 (OCT-4) (+)SOX2 (+)
PluriTest: Pluripotency score: 40.022
Novelty score: 1.34
Microarray data: http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Genotyping
Genome-wide analysis:
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ123017
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: Essential E8®
Passage method: EDTA
Matrix: Vitronectin
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-GesellschaftПараметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method Sendai virus description age (65-69) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
WTSIi005-B 66540109-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Wellcome Trust Sanger InstituteCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Location of primary tissue procurement: Arm
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: WTSIi005-A
Characterization
Analysis of Undifferentiated Cells
Marker expression: POU5F1 (OCT-4)(+)SOX2 (+)NANOG(+)
PluriTest: Pluripotency score: 45.034
Novelty score: 1.353
Microarray data: http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Genotyping
Genome-wide analysis:
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266756
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: Essential E8®
Passage method: EDTA
Matrix: Vitronectin
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-GesellschaftПараметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method Sendai virus description age (65-69) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
WTSIi006-A 66540076-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Wellcome Trust Sanger InstituteCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Location of primary tissue procurement: Arm
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: WTSIi006-B
Characterization
Analysis of Undifferentiated Cells
Marker expression: POU5F1 (OCT-4)(+)SOX2 (+)NANOG (+)
PluriTest: Pluripotency score: 22.113
Novelty score: 1.264
Microarray data: http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Genotyping
Genome-wide analysis:
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ122973
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: Essential E8®
Passage method: EDTA
Matrix: Vitronectin
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-GesellschaftПараметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method Sendai virus description age (70-74) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
WTSIi006-B 66540110-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Wellcome Trust Sanger InstituteCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Location of primary tissue procurement: Arm
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: WTSIi006-A
Characterization
nalysis of Undifferentiated Cells
Marker expression: NANOG(+)SOX2(+)POU5F1 (OCT-4)(+)
PluriTest: Pluripotency score: 24.515
Novelty score: 1.371
Microarray data: http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Genotyping
Genome-wide analysis:
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ122985
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: Essential E8®
Passage method: EDTA
Matrix: Vitronectin
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-GesellschaftПараметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method Sendai virus description age (70-74) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
WTSIi007-A 66540077-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Wellcome Trust Sanger InstituteCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Location of primary tissue procurement: Arm
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Characterization
Analysis of Undifferentiated Cells
Marker expression: POU5F1 (OCT-4)(+)SOX2 (+)NANOG (+)
PluriTest: Pluripotency score: 55.797
Novelty score: 1.384
Microarray data: http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Genotyping
Genome-wide analysis:
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ122968
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: Essential E8®
Passage method: EDTA
Matrix: Vitronectin
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-GesellschaftПараметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method Sendai virus description age (55-59) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
WTSIi008-A 66540078-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Wellcome Trust Sanger InstituteCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Location of primary tissue procurement: Arm
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Characterization
Analysis of Undifferentiated Cells
Marker expression: POU5F1 (OCT-4)(+) NANOG(+)SOX2(+)
PluriTest: Pluripotency score: 33.433
Novelty score: 1.345
Microarray data: http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Genotyping
Genome-wide analysis:
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ123015
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA)
- Cell Line Information Pack (CLIP)
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: Essential E8®
Passage method: EDTA
Matrix: Vitronectin
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-GesellschaftПараметрыQuality Level 100 biological source human dermis (fibroblast) reprogramming method Sendai virus description age (25-29) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену
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С 2010 года мы поставляем оборудование с заводов Европы. Берем на себя все — от подбора оборудования до внедрения на предприятии
Все сотрудники имеют высшее образование, закончили ведущие химические вузы страны, такие как РХТУ им Менделеева.
У большинства компаний срок ожидания составляет 10-12 недель.
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В случае любых неполадок за свой счет выполним ремонт в сервисном центре или на заводе-изготовителе. Или бесплатно заменим прибор на новый.
Производим пуско-наладку оборудования, валидацию, обучение сотрудников. Если нужно, привлекаем инженеров с заводов- изготовителей.
