Cell Lines & Specialty Cell Culture
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UKKi020-B 66540562-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 3
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Is the reprogramming vector detectable: No
Merthods used: pcr
Notes on reprogramming vector detection: episomal vector cassette absence
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
CD90 Yes -
FOXD3 Yes -
NANOG Yes - -
OCT 4 Yes - -
SOX2 Yes -
SSEA-4 Yes - -
TRA 1-80 Yes - -
SSEA-1 Yes -
DNMT3b Yes -
Morphology:
morphology pictures of iPS cell line NP0100-8 before banking and of the thawing control
morphology pictures of iPS cell line NP0100-8 before banking and of the thawing control
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Unknown
Morphology:
expression of NESTIN in differentiated iPS cell line NP0100-8
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
expression of SOX17 in differentiated iPS cell line NP0100-8
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
expression of BRACHYURY in differentiated iPS cell line NP0100-8
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 26
Cell line karyotype: No larger chromosomal aberrations observed
Karyotyping method: Molecular karyotyping by SNP array
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: Essential E8®
Passage Number banked (pre-EBiSC): 26
No. Vials banked (pre-EBiSC): 25Legal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-GesellschaftПараметрыbiological source human peripheral blood reprogramming method episomal description age (30-34) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi020-C 66540563-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Is the reprogramming vector detectable: No
Merthods used: pcr
Notes on reprogramming vector detection: episomal vector cassette absence
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-4 Yes - -
TRA 1-80 Yes - -
FOXD3 Yes -
DNMT3b Yes -
SSEA-1 Yes -
CD90 Yes -
NANOG Yes - -
SOX2 Yes -
OCT 4 Yes - -
Morphology:
morphology pictures of iPS cell line NP0100-10E before banking and of the thawing control
morphology pictures of iPS cell line NP0100-10E before banking and of the thawing control
morphology pictures of iPS cell line NP0100-10E before banking and of the thawing control
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
expression of NESTIN in differentiated iPS cell line NP0100-10
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
expression of SOX17 in differentiated iPS cell line NP0100-10
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
expression of BRACHYURY in differentiated iPS cell line NP0100-10
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 14
Cell line karyotype: Chr20: Duplication in 20q11.21
Karyotyping method: Molecular karyotyping by SNP array
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: Essential E8®
Passage Number banked (pre-EBiSC): 27
No. Vials banked (pre-EBiSC): 20Legal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-GesellschaftПараметрыbiological source human peripheral blood reprogramming method episomal description age (30-34) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi020-D 66540564-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Is the reprogramming vector detectable: No
Merthods used: pcr
Notes on reprogramming vector detection: episomal vector cassette absence
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-4 Yes - -
SOX2 Yes -
TRA 1-80 Yes - -
SSEA-1 Yes -
FOXD3 Yes -
NANOG Yes - -
CD90 Yes -
OCT 4 Yes - -
DNMT3b Yes -
Morphology:
morphology pictures of iPS cell line NP0100-11 before banking and of the thawing control
morphology pictures of iPS cell line NP0100-11 before banking and of the thawing control
morphology pictures of iPS cell line NP0100-11 before banking and of the thawing control
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
expression of NESTIN in differentiated iPS cell line NP0100-11
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
expression of SOX17 in differentiated iPS cell line NP0100-11
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
expression of BRACHYURY in differentiated iPS cell line NP0100-11
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 15
Cell line karyotype: No larger chromosomal aberrations to be reported.
Karyotyping method: Molecular karyotyping by SNP array
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: Essential E8®
Passage Number banked (pre-EBiSC): 18
No. Vials banked (pre-EBiSC): 25Legal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-GesellschaftПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (30-34) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi021-A 66540547-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Location of primary tissue procurement: Zone of skin
Tissue collection year: 2016
Passage number reprogrammed: 3
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Is the reprogramming vector detectable: No
Merthods used: pcr
Notes on reprogramming vector detection: episomal vector cassette absent
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-4 Yes - -
SSEA-1 Yes -
TRA 1-80 Yes - -
CD90 Yes -
SOX2 Yes -
DNMT3b Yes -
NANOG Yes - -
OCT 4 Yes - -
FOXD3 Yes -
Morphology:
mophology pictures of NP0105-2 before banking and of the thawing control
mophology pictures of NP0105-2 before banking and of the thawing control
Differentiation potency
Ectoderm:
Ectoderm
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
expression of SOX17 in differentiated iPS cell line NP0105-2
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
expression of BRACHYURY in differentiated iPS cell line NP0105-2
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 31
Cell line karyotype: Chr01: Trisomy of whole chromosome; Chr11: Deletion of telomeric region of long arm; Chr22: Onset of mosaicism at the telomeric end of long arm
Karyotyping method: Molecular karyotyping by SNP array
Image / ResultLinkageAdditional Cell Line Info,
EBiSC Access Use Agreement (EAUA),
Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: Essential E8®
Passage Number banked (pre-EBiSC): 30
No. Vials banked (pre-EBiSC): 30Legal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-GesellschaftПараметрыbiological source human dermis (fibroblast) reprogramming method episomal description age (30-34) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi021-B 66540565-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Location of primary tissue procurement: Zone of skin
Tissue collection year: 2016
Passage number reprogrammed: 3
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Is the reprogramming vector detectable: No
Merthods used: pcr
Notes on reprogramming vector detection: episomal vector cassette absent
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
CD30 Yes -
TRA 1-80 Yes - -
DNMT3b Yes -
CD90 Yes -
SSEA-4 Yes - -
SSEA-1 Yes -
NANOG Yes - -
OCT 4 Yes - -
FOXD3 Yes -
SOX2 Yes -
EpiPluriScore:
Score: 0,24
Marker Present Absent
mCpG
OCT4
Report:
Epi_Pluri_score_2017.jpg
Epi_pluri_score_result_20170621.pdf - Analysis Report Epi Pluri Score
Morphology:
hiPS NP0105-19 before banking and after thawing
hiPS NP0105-19 on different days post thaw
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin No
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 15
Cell line karyotype: Chr07: Larger duplication (20Mbp) mosaic (80%) on long arm.
Karyotyping method: Molecular karyotyping by SNP array
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: other
Culture base medium: StemFlex™ Basal Medium
Main protein source: -
Passage Number banked (pre-EBiSC): 21
No. Vials banked (pre-EBiSC): 25Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
StemFlex is a trademark of Life Technologies CorporationПараметрыbiological source human dermis (fibroblast) reprogramming method episomal description age (30-34) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi022-A 66540484-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Location of primary tissue procurement: Zone of skin
Tissue collection year: 2016
Passage number reprogrammed: 3
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Is the reprogramming vector detectable: No
Merthods used: pcr
Notes on reprogramming vector detection: episomal vector cassette absent
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-80 Yes - -
SOX2 Yes -
SSEA-1 Yes -
OCT 4 Yes - -
SSEA-4 Yes - -
FOXD3 Yes -
DNMT3b Yes -
NANOG Yes - -
Morphology:
morphology pictures of iPS cell line NP0106-34 before banking and of the thawing control
morphology pictures of iPS cell line NP0106-34 before banking and of the thawing control
morphology pictures of iPS cell line NP0106-34 before banking and of the thawing control
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
expression of NESTIN in differentiated iPS cell line NP0106-34
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
expression of SOX17 in differentiated iPS cell line NP0106-34
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
expression of BRACHYURY in differentiated iPS cell line NP0106-34
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 25
Cell line karyotype: Chr03: Large region of loss of homozygosity on the telometric end of the long arm; Chr20: Deletion of short arm an a duplication of the long arm
Karyotyping method: Molecular karyotyping by SNP array
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: Essential E8®
Passage Number banked (pre-EBiSC): 25
No. Vials banked (pre-EBiSC): 20Legal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-GesellschaftПараметрыbiological source human dermis (fibroblast) reprogramming method episomal description age (30-34) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi022-C 66540568-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Location of primary tissue procurement: Zone of skin
Tissue collection year: 2016
Passage number reprogrammed: 3
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Is the reprogramming vector detectable: No
Merthods used: pcr
Notes on reprogramming vector detection: episomal vector cassette absent
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SOX2 Yes -
DNMT3b Yes -
OCT 4 Yes - -
SSEA-1 Yes -
NANOG Yes - -
SSEA-4 Yes - -
TRA 1-80 Yes - -
FOXD3 Yes -
CD90 Yes -
Morphology:
morphology pictures of iPS cell line NP0106-10A before banking and of the thawing control
morphology pictures of iPS cell line NP0106-10A before banking and of the thawing control
morphology pictures of iPS cell line NP0106-10A before banking and of the thawing control
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
expression of NESTIN in differentiated iPS cell line NP0106-10
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Unknown
Morphology:
expression of SOX17 in differentiated iPS cell line NP0106-10
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
expression of BRACHYURY in differentiated iPS cell line NP0106-10
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 17
Cell line karyotype: Chr10: 3Mbp large duplication mosaicism on short arm.
Karyotyping method: Molecular karyotyping by SNP array
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: Essential E8®
Passage Number banked (pre-EBiSC): 27
No. Vials banked (pre-EBiSC): 25Legal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-GesellschaftПараметрыbiological source human dermis (fibroblast) reprogramming method episomal description age (30-34) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi022-D 66540579-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Location of primary tissue procurement: Zone of skin
Tissue collection year: 2016
Passage number reprogrammed: 3
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Is the reprogramming vector detectable: Yes
Merthods used: pcr
Notes on reprogramming vector detection: episomal vector cassette absent
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
FOXD3 Yes -
NANOG Yes - -
CD90 Yes -
DNMT3b Yes -
SOX2 Yes -
OCT 4 Yes - -
SSEA-1 Yes -
SSEA-4 Yes - -
TRA 1-80 Yes - -
Morphology:
morphology pictures of iPS cell line NP0106-5 before banking and of the thawing control
morphology pictures of iPS cell line NP0106-5 before banking and of the thawing control
morphology pictures of iPS cell line NP0106-5 before banking and of the thawing control
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
expression of Nestin in differentiated iPS cell line NP0106-5
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
expression of SOX17 in differentiated iPS cell line NP0106-5
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
expression of BRACHYURY in differentiated iPS cell line NP0106-5
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 20
Cell line karyotype: No larger chromosomal aberrations to be reported.
Karyotyping method: Molecular karyotyping by SNP array
Image / Result
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: Essential E8®/Essential E8® Flex
Passage method: EDTA
Matrix: Vitronectin
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-GesellschaftПараметрыbiological source human dermis (fibroblast) reprogramming method episomal description age (30-34) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi023-A 66540485-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Notes on reprogramming vector detection: sendai virus absent
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-80 Yes - -
SSEA-1 Yes -
NANOG Yes - -
SSEA-4 Yes - -
FOXD3 Yes -
DNMT3b Yes -
OCT 4 Yes - -
Morphology:
pictures of NP0126-1 before bankung and of the thawing control
pictures of NP0126-1 before bankung and of the thawing control
pictures of NP0126-1 before bankung and of the thawing control
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
expression of NESTIN in differentiated iPS cell line NP0126-1
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
expression of SOX17 in differentiated iPS cell line NP0126
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
expression of BRACHYURY in differentiated iPS cell line NP0126
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 36
Cell line karyotype: No larger chromosomal aberrations observed
Karyotyping method: Molecular karyotyping by SNP array
Image / Result
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: -
Passage method: -
Matrix: -
CO2 concentration: -
O2 concentration: -
Temperature: -Legal InformationEBiSC is a trademark of Fraunhofer-GesellschaftПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (50-54) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) familial long QT syndrome shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi023-B 66540486-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Notes on reprogramming vector detection: sendai virus absent
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
FOXD3 Yes -
DNMT3b Yes -
NANOG Yes - -
SOX2 Yes -
SSEA-1 Yes -
OCT 4 Yes - - -
CD90 Yes -
TRA 1-80 Yes - -
SSEA-4 Yes - -
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
expression of NESTIN in differentiated iPS cell line NP0126-5
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
expression of SOX17 in differentiated iPS cell line NP0126-5
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
expression of BRACHYURY in differentiated iPS cell line NP0126-5
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 39
Cell line karyotype: No larger chromosomal aberrations observed
Karyotyping method: Molecular karyotyping by SNP array
Image / Result
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: -
Passage method: -
Matrix: -
CO2 concentration: -
O2 concentration: -
Temperature: -Legal InformationEBiSC is a trademark of Fraunhofer-GesellschaftПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (50-54) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) familial long QT syndrome shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi023-C 66540487-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Notes on reprogramming vector detection: sendai virus absent
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
CD90 Yes -
SSEA-1 Yes -
FOXD3 Yes -
SSEA-4 Yes - -
NANOG Yes - -
SOX2 Yes -
OCT 4 Yes - -
TRA 1-80 Yes - -
DNMT3b Yes -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
expression of NESTIN in differentiated NP0126-6
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
expression of SOX17 in differentiated NP0126-6
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
expression of BRACHYURY in differentiated NP0126-6
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 49
Cell line karyotype: no larger aberrations observed
Karyotyping method: Molecular karyotyping by SNP array
Image / Result
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: -
Passage method: -
Matrix: -
CO2 concentration: -
O2 concentration: -
Temperature: -Legal InformationEBiSC is a trademark of Fraunhofer-GesellschaftПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (50-54) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) familial long QT syndrome shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi024-A 66540488-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Notes on reprogramming vector detection: sendai virus absent
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
DNMT3b Yes -
SOX2 Yes -
TRA1-80 Yes - -
NANOG Yes -
OCT 4 Yes - -
FOXD3 Yes -
SSEA-4 Yes - -
SSEA-1 Yes -
CD90 Yes -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
expression of NESTIN in differentiated NP0133-5
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
expression of SOX17 in differentiated NP0133-5
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
expression of BRACHYURY in differentiated NP0133-5
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 37
Cell line karyotype: No larger chromosomal aberrations to be reported
Karyotyping method: Molecular karyotyping by SNP array
Image / Result
Genome-wide analysis:
SNP typing array
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: -
Passage method: -
Matrix: -
CO2 concentration: -
O2 concentration: -
Temperature: -Legal InformationEBiSC is a trademark of Fraunhofer-GesellschaftПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (30-34) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Brugada syndrome shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi024-B 66540489-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Notes on reprogramming vector detection: sendai virus absent
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SOX2 Yes -
CD90 Yes -
NANOG Yes - -
FOXD3 Unknown -
TRA 1-80 Yes - -
SSEA-4 Yes - -
DNMT3b Unknown -
OCT 4 Yes - -
SSEA-1 Yes -
Morphology:
thawing control of NP0133-1
thawing control of NP0133-1
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
expression of NESTIN in differentiated iPS cell line NP0133-1
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
expression of SOX17 in differentiated iPS cell line NP0133-1
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
expression of BRACHYURY in differentiated iPS cell line NP0133-1
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 32
Cell line karyotype: No larger chromosomal aberrations observed
Karyotyping method: Molecular karyotyping by SNP array
Image / Result
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: -
Passage method: -
Matrix: -
CO2 concentration: -
O2 concentration: -
Temperature: -Legal InformationEBiSC is a trademark of Fraunhofer-GesellschaftПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (30-34) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Brugada syndrome shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi024-C 66540490-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Notes on reprogramming vector detection: Sendai virus absent
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG Yes - -
OCT4 Yes - -
FOXD3 Yes -
CD90 Yes -
DNMT3b Yes -
SSEA-1 Yes -
SOX2 Yes -
SSEA-4 Yes - -
TRA 1-80 Yes - -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
expression of NESTIN in differentiated iPS cell line NP0133-4
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
expression of SOX17 in differentiated iPS cell line NP0133-4
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
expression of BRACHYURY in differentiated iPS cell line NP0133-4
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 32
Cell line karyotype: No larger chromosomal aberrations observed
Karyotyping method: Molecular karyotyping by SNP array
Image / Result
Genome-wide analysis:
SNP typing array
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: -
Passage method: -
Matrix: -
CO2 concentration: -
O2 concentration: -
Temperature: -Legal InformationEBiSC is a trademark of Fraunhofer-GesellschaftПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (30-34) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Brugada syndrome shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi025-A 66540491-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Notes on reprogramming vector detection: sendai virus absent
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-4 Yes - -
FOXD3 Yes -
OCT 4 Yes - -
SSEA-1 Yes -
SOX2 Yes -
DNMT3b Yes -
NANOG Yes - -
CD90 Yes -
TRA 1-80 Yes - -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
expression of NESTIN in differentiated iPS cell line NP0135-1
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
expression of SOX17 in differentiated iPS cell line NP0135-1
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
expression of BRACHYURY in differentiated iPS cell line NP0135-1
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 30
Cell line karyotype: No larger chromosomal aberrations observed
Karyotyping method: Molecular karyotyping by SNP array
Image / Result
Genome-wide analysis:
SNP typing array
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: Essential E8®
Passage Number banked (pre-EBiSC): 38
No. Vials banked (pre-EBiSC): 20Legal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-GesellschaftПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (40-44) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) hypertrophic cardiomyopathy shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi025-B 66540492-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Notes on reprogramming vector detection: sendai virus absent
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
OCT 4 Yes - -
TRA 1-80 Yes - -
NANOG Yes - -
FOXD3 Yes -
SOX2 Yes -
SSEA-1 Yes -
SSEA-4 Yes - -
DNMT3b Yes -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
expression of NESTIN in differentiated iPS cell line NP0135-2
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
expression of SOX17 in differentiated iPS cell line NP0135-2
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
expression of BRACHYURY in differentiated iPS cell line NP0135-2
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 38
Cell line karyotype: No larger chromosomal aberrations observed.
Karyotyping method: Molecular karyotyping by SNP array
Image / Result
Genome-wide analysis:
SNP typing array
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: -
Passage method: -
Matrix: -
CO2 concentration: -
O2 concentration: -
Temperature: -Legal InformationEBiSC is a trademark of Fraunhofer-GesellschaftПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (40-44) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) hypertrophic cardiomyopathy shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi025-C 66540493-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Notes on reprogramming vector detection: sendai virus absent
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-4 Yes - -
SOX2 Yes -
TRA 1-80 Yes - -
SSEA-1 Yes -
OCT 4 Yes - -
DNMT3b Yes -
FOXD3 Yes -
NANOG Yes - -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
expression of NESTIN in differentiated iPS cell line NP0135-7
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
expression of SOX17 in differentiated iPS cell line NP0135-7
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
expression of BRACHYURY in differentiated iPS cell line NP0135-7
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 31
Cell line karyotype: No larger chromosomal aberrations observed.
Karyotyping method: Molecular karyotyping by SNP array
Image / Result
Genome-wide analysis:
SNP typing array
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: -
Passage method: -
Matrix: -
CO2 concentration: -
O2 concentration: -
Temperature: -Legal InformationEBiSC is a trademark of Fraunhofer-GesellschaftПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (40-44) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) hypertrophic cardiomyopathy shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi026-A 66540621-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-4 Yes -
TRA 1-80 Yes - -
DNMT3b Yes -
FOXD3 Yes -
CD30 Yes -
NANOG Yes - -
OCT 4 Yes - -
SOX2 Yes -
SSEA-1 Yes -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
Protocol or reference:
Trilineage_differentiation_protocol.pdf
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 21
Cell line karyotype: no larger aberrations to be reported. Chr10: small mosaicism on long aem
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: other
Culture base medium: StemFlex™
Main protein source: -
Passage Number banked (pre-EBiSC): 22
No. Vials banked (pre-EBiSC): 25Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
StemFlex is a trademark of Life Technologies CorporationПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (25-29) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi026-B 66540595-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-4 Yes - -
SSEA-1 Yes -
TRA 1-80 Yes - -
OCT 4 Yes - -
NANOG Yes - -
SOX2 Yes -
CD30 Yes -
FOXD3 Yes -
DNMT3b Yes -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
Protocol or reference:
Trilineage_differentiation_protocol.pdf
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 24
Cell line karyotype: No larger chromosomal aberrations to be reported.
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: other
Culture base medium: StemFlex™
Main protein source: -
Passage Number banked (pre-EBiSC): 27
No. Vials banked (pre-EBiSC): 25Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
StemFlex is a trademark of Life Technologies CorporationПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (25-29) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi026-C 66540596-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
FOXD3 Yes -
CD30 Yes -
OCT 4 Yes - -
NANOG Yes - -
DNMT3b Yes -
SSEA-4 Yes - -
TRA 1-80 Yes - -
SOX2 Yes -
SSEA-1 Yes -
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
Protocol or reference:
Trilineage_differentiation_protocol.pdf
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 23
Cell line karyotype: no larger aberrations to be reported. Chr10: small mosaicism on long arm
Karyotyping method: Molecular karyotyping by SNP array
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: other
Culture base medium: StemFlex
Main protein source: -
Passage Number banked (pre-EBiSC): 27
No. Vials banked (pre-EBiSC): 25Legal InformationEBiSC is a trademark of Fraunhofer-GesellschaftПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (25-29) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi027-A 66540622-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG Yes - -
OCT 4 Yes - -
CD30 Yes -
FOXD3 Yes -
DNMT3b Yes -
SSEA-4 Yes - -
TRA 1-80 Yes - -
SSEA-1 Yes -
SOX2 Yes -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Unknown
Morphology:
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
Protocol or reference:
Trilineage_differentiation_protocol.pdf
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 9
Cell line karyotype: no larger chromosomal aberrations to be reported
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: other
Culture base medium: StemFlex™
Main protein source: -
Passage Number banked (pre-EBiSC): 24
No. Vials banked (pre-EBiSC): 25Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
StemFlex is a trademark of Life Technologies CorporationПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (25-29) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi027-B 66540623-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
CD30 Yes -
SOX2 Yes -
TRA 1-80 Yes - -
SSEA-4 Yes - -
OCT 4 Yes - -
NANOG Yes - -
FOXD3 Yes -
SSEA-1 Yes -
DNMT3b Yes -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
Protocol or reference:
Trilineage_differentiation_protocol.pdf
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 18
Cell line karyotype: no larger aberrations to be reported
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: other
Culture base medium: StemFlex™
Main protein source: -
Passage Number banked (pre-EBiSC): 23
No. Vials banked (pre-EBiSC): 25Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
StemFlex is a trademark of Life Technologies CorporationПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (25-29) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi027-C 66540624-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
FOXD3 Yes -
CD30 Yes -
DNMT3b Yes -
TRA 1-80 Yes - -
SOX2 Yes -
NANOG Yes - -
OCT 4 Yes - -
SSEA-4 Yes - -
SSEA-1 Yes -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
Protocol or reference:
Trilineage_differentiation_protocol.pdf
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 17
Cell line karyotype: no larger chromosomal aberrations to be reported
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: other
Culture base medium: StemFlex™
Main protein source: -
Passage Number banked (pre-EBiSC): 22
No. Vials banked (pre-EBiSC): 25Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
StemFlex is a trademark of Life Technologies CorporationПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (25-29) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi028-A 66540625-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SOX2 Yes -
TRA 1-80 Yes - -
SSEA-4 Yes - -
SSEA-1 Yes -
FOXD3 Yes -
NANOG Yes - -
OCT 4 Yes - -
DNMT3b Yes -
CD30 Yes -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Unknown
Morphology:
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
Protocol or reference:
Trilineage_differentiation_protocol.pdf
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 10
Cell line karyotype: no larger chromosomal aberrations to be reported
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: other
Culture base medium: StemFlex™
Main protein source: -
Passage Number banked (pre-EBiSC): 23
No. Vials banked (pre-EBiSC): 25Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
StemFlex is a trademark of Life Technologies CorporationПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (30-34) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi028-B 66540626-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
FOXD3 Yes -
NANOG Yes - -
SSEA-1 Yes -
CD30 Yes -
DNMT3b Yes -
SOX2 Yes -
TRA 1-80 Yes - -
OCT 4 Yes - -
SSEA-4 Yes - -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
Protocol or reference:
Trilineage_differentiation_protocol.pdf
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 10
Cell line karyotype: no larger chromosomal aberrations to be reported
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: other
Culture base medium: StemFlex™
Main protein source: -
Passage Number banked (pre-EBiSC): 19
No. Vials banked (pre-EBiSC): 25Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
StemFlex is a trademark of Life Technologies CorporationПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (30-34) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi028-C 66540627-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
CD30 Yes -
NANOG Yes - -
SOX2 Yes -
OCT 4 Yes - -
DNMT3b Yes -
SSEA-1 Yes -
FOXD3 Yes -
TRA 1-80 Yes - -
SSEA-4 Yes - -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
Protocol or reference:
Trilineage_differentiation_protocol.pdf
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 27
Cell line karyotype: no larger chromosomal aberrations to be reported. Chr05: small deletion mosaicism on long arm
Karyotyping method: Molecular karyotyping by SNP array
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: other
Culture base medium: StemFlex™
Main protein source: -
Passage Number banked (pre-EBiSC): 24
No. Vials banked (pre-EBiSC): 25Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
StemFlex is a trademark of Life Technologies CorporationПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (30-34) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi029-A 66540872-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2017
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: immune_staining, pcr
Notes on reprogramming vector detection: sendai virus absent
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-4 Yes - -
TRA 1-80 Yes -
SOX2 Yes -
OCT 4 Yes - -
NANOG Yes - -
FOXD3 Yes -
DNMT3b Yes -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 18
Cell line karyotype: No larger chromosomal aberrations to be reported.
Karyotyping method: Molecular karyotyping by SNP array
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: other
Culture base medium: StemFlex™
Main protein source: Albumine
Passage Number banked (pre-EBiSC): 16
No. Vials banked (pre-EBiSC): 25Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
StemFlex is a trademark of Life Technologies CorporationПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (20-24) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) prolonged QT interval shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi029-B 66540873-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2017
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-80 Yes - -
FOXD3 Yes -
OCT 4 Yes - -
NANOG Yes - -
DNMT3b Yes -
SOX2 Yes -
SSEA-1 Yes -
SSEA-4 Yes - -
Morphology:
Morphology pictures of the Masterbank and the thawing control do
Morphology pictures of the thawing control d1 and d2
Morphology pictures of the thawing control d3
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 17
Cell line karyotype: No larger chromosomal aberrations to be reported.
Karyotyping method: Molecular karyotyping by SNP array
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: other
Culture base medium: StemFlex™
Main protein source: Albumine
Passage Number banked (pre-EBiSC): 19
No. Vials banked (pre-EBiSC): 25Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
StemFlex is a trademark of Life Technologies CorporationПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (20-24) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) prolonged QT interval shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi029-C 66540874-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2017
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-4 Yes - -
SSEA-1 Yes -
FOXD3 Yes -
TRA 1-80 Yes - -
OCT 4 Yes - -
SOX2 Yes -
NANOG Yes - -
DNMT3b Yes -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
Protocol or reference:
STEMdiff_Trilienage_protocol.pdf
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 21
Cell line karyotype: No larger chromosomal aberrations to be reported.
Karyotyping method: Molecular karyotyping by SNP array
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: other
Culture base medium: StemFlex™
Main protein source: Albumine
Passage Number banked (pre-EBiSC): 19
No. Vials banked (pre-EBiSC): 25Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
StemFlex is a trademark of Life Technologies CorporationПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (20-24) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) prolonged QT interval shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi030-A 66540875-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG Yes - -
FOXD3 Yes -
DNMT3b Yes -
TRA 1-80 Yes -
SSEA-4 Yes - -
OCT4 Yes - -
SOX2 Yes -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
Protocol or reference:
STEMdiff_Trilineage_protocol.pdf
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 15
Cell line karyotype: No larger chromosomal aberrations to be reported.
Karyotyping method: Molecular karyotyping by SNP array
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: other
Culture base medium: StemFlex™
Main protein source: Albumine
Passage Number banked (pre-EBiSC): 18
No. Vials banked (pre-EBiSC): 20Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
StemFlex is a trademark of Life Technologies CorporationПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (65-69) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Brugada syndrome shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi030-B 66540876-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: immune_staining, pcr
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
DNMT3b Yes -
FOXD3 No -
SSEA-4 Yes - -
OCT 4 Yes - -
SOX2 No -
SSEA-1 Yes -
NANOG Yes - -
TRA 1-80 Yes - -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
p24
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
p24
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
p24
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 18
Cell line karyotype: No larger chromosomal aberrations to be reported.
Karyotyping method: Molecular karyotyping by SNP array
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: other
Culture base medium: STEMFLEX™
Main protein source: Albumine
Passage Number banked (pre-EBiSC): 20
No. Vials banked (pre-EBiSC): 25Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
StemFlex is a trademark of Life Technologies CorporationПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (N/A) Торговая марка EBiSC™ gender N/A growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Brugada syndrome shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi030-C 66540877-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SOX2 Yes -
TRA 1-80 Yes -
OCT 4 Yes - -
SSEA-4 Yes - -
NANOG Yes - -
DNMT3b Yes -
FOXD3 Yes -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Unknown
Morphology:
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
Protocol or reference:
STEMdiff_Trilineage_protocol.pdf
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 18
Cell line karyotype: No larger chromosomal aberrations to be reported.
Karyotyping method: Molecular karyotyping by SNP array
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: other
Culture base medium: StemFlex™
Main protein source: Albumine
Passage Number banked (pre-EBiSC): 17
No. Vials banked (pre-EBiSC): 20Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
StemFlex is a trademark of Life Technologies CorporationПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (N/A) Торговая марка EBiSC™ gender N/A growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Brugada syndrome shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi031-A 66540878-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
DNMT3b Yes -
SSEA-4 Yes - -
SOX2 Yes -
TRA 1-80 Yes -
OCT 4 Yes - -
FOXD3 Yes -
NANOG Yes - -
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 16
Cell line karyotype: No larger chromosomal aberrations to be reported. Chr20: Duplication in q11.21: Cell culture adaptation.
Karyotyping method: Molecular karyotyping by SNP array
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: other
Culture base medium: StemFlex™
Main protein source: Albumine
Passage Number banked (pre-EBiSC): 19
No. Vials banked (pre-EBiSC): 25Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
StemFlex is a trademark of Life Technologies CorporationПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (55-59) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) hypertrophic cardiomyopathy shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi031-B 66540879-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SOX2 Yes -
OCT 4 Yes - -
NANOG Yes - -
TRA 1-80 Yes -
SSEA-4 Yes - -
DNMT3b Yes -
FOXD3 Yes -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Morphology:
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 18
Cell line karyotype: No larger chromosomal aberrations to be reported; Chr20: Duplication in q11.21.
Karyotyping method: Molecular karyotyping by SNP array
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: other
Culture base medium: StemFlex™
Main protein source: Albumine
Passage Number banked (pre-EBiSC): 21
No. Vials banked (pre-EBiSC): 25Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
StemFlex is a trademark of Life Technologies CorporationПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (60-64) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) hypertrophic cardiomyopathy shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi031-C 66540880-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SOX2 Yes -
SSEA-4 Yes - -
DNMT3b Yes -
TRA 1-80 Yes -
NANOG Yes - -
OCT 4 Yes - -
FOXD3 Yes -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Unknown
Morphology:
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 15
Cell line karyotype: No larger chromosomal aberrations to be reported; Chr20: Duplication in q11.21
Karyotyping method: Molecular karyotyping by SNP array
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: other
Culture base medium: StemFlex™
Main protein source: Albumine
Passage Number banked (pre-EBiSC): 18
No. Vials banked (pre-EBiSC): 25Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
StemFlex is a trademark of Life Technologies CorporationПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (N/A) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) hypertrophic cardiomyopathy shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi032-A 66540881-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
OCT 4 Yes - -
FOXD3 Yes -
NANOG Yes - -
SOX2 Yes -
SSEA-4 Yes - -
TRA1-80 Yes -
DNMT3b Yes -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 22
Cell line karyotype: No larger chromosomal aberrations to be reported; Chr12: Duplication of telomeric
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: other
Culture base medium: StemFlex™
Main protein source: -
Passage Number banked (pre-EBiSC): 23
No. Vials banked (pre-EBiSC): 25Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
StemFlex is a trademark of Life Technologies CorporationПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (40-44) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi032-B 66540882-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG Yes - -
SSEA-4 Yes - -
SSEA-1 Yes -
SOX2 Yes -
FOXD3 Yes -
TRA 1-81 Yes - -
OCT4 Yes - -
DNMT3b Yes -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 23
Cell line karyotype: No larger chromosomal aberrations to be reported; Chr12: Duplication of telomeric
Karyotyping method: Molecular karyotyping by SNP array
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: other
Culture base medium: StemFlex™
Main protein source: -
Passage Number banked (pre-EBiSC): 20
No. Vials banked (pre-EBiSC): 21Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
StemFlex is a trademark of Life Technologies CorporationПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (40-44) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi032-C 66540883-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
DNMT3b Yes -
SOX2 Yes -
TRA 1-80 Yes - -
SSEA-1 Yes -
SSEA-4 Yes - -
NANOG Yes - -
FOXD3 Yes -
OCT 4 Yes - -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
Protocol or reference:
STEMdiff_Trilineage_protocol.pdf
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 21
Cell line karyotype: No larger chromosomal aberrations to be reported.
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: other
Culture base medium: StemFlex™
Main protein source: -
Passage Number banked (pre-EBiSC): 22
No. Vials banked (pre-EBiSC): 25Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
StemFlex is a trademark of Life Technologies CorporationПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (40-44) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi001-A 66540039-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Location of primary tissue procurement: University of Newcastle, Biobank
Tissue collection date: June 24, 2013
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Characterization
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Endoderm: In vitro spontaneous differentiation
Mesoderm: In vitro spontaneous differentiation
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method Sendai virus description age (45-49) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) retinitis pigmentosa shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi002-A 66540040-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Characterization
Analysis of Undifferentiated Cells
Marker expression: SSEA-4(+)TRA 1-60(+)SSEA-1 (+)NANOG(+)
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Endoderm: In vitro spontaneous differentiation
Mesoderm: In vitro spontaneous differentiation
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Cell line karyotype: CytoSNP
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method Sendai virus description age (50-54) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) retinitis pigmentosa shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi004-A 66540017-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Derivation
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression: NANOG(+)SSEA-4(+)TRA 1-60( +)
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Endoderm: In vitro spontaneous differentiation
Mesoderm: In vitro spontaneous differentiation
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Cell line karyotype: CytoSNP
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (45-49) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) retinitis pigmentosa shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi005-A 66540020-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Characterization
Analysis of Undifferentiated Cells
Marker expression: SSEA-4(+)TRA 1-60(+)NANOG (+)
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Endoderm: In vitro spontaneous differentiation
Mesoderm: In vitro spontaneous differentiation
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Cell line karyotype: CytoSNP
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (50-54) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) retinitis pigmentosa shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi017-A 66540057-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-60(+)SSEA-4 (+)SSEA-1(-)POU5F1 (OCT-4)(+)NANOG(+)
hPSC Scorecard: Positive Negative,Self-renewal,Endoderm,Mesoderm,Ectoderm
Differentiation potency
Ectoderm: In vivo teratoma,In vitro spontaneous differentiation
Endoderm: In vivo teratoma,In vitro spontaneous differentiation
Mesoderm: In vivo teratoma,In vitro spontaneous differentiation
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Cell line karyotype: two small regions of homozygosity
Karyotyping method: CytoSNP
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (15-19) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) aplastic anemia shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi018-A 66540012-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-60(+)SSEA-1(-)POU5F1 (OCT-4)(+)SSEA-4(+)
Differentiation potency
Ectoderm: In vivo teratoma,In vitro spontaneous differentiation
Endoderm: In vivo teratoma,In vitro spontaneous differentiation
Mesoderm: In vivo teratoma,In vitro spontaneous differentiation
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Cell line karyotype: trisomy chromosome 3
Karyotyping method: CytoSNP
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method Sendai virus description age (10-14) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) aplastic anemia shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi019-A 66540021-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Characterization
Analysis of Undifferentiated Cells
Marker expression: NANOG(+),SSEA-1 (-),TRA 1-60(+),SSEA-4 (+)
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Endoderm: In vitro spontaneous differentiation
Mesoderm: In vitro spontaneous differentiation
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Cell line karyotype: no clinical significant imbalance was detected
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (20-24) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) anemia shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi021-A 66540174-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Passage number reprogrammed: P4
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: UNEWi021-B, UNEWi021-C
Characterization
Analysis of Undifferentiated Cells
Marker expression: NANOG(+)SSEA-4 (+)SSEA1 (-)TRA 1-60(+)
Differentiation potency
Ectoderm:
Retinal Pigment Epithelium
In vitro spontaneous differentiation
In vitro directed differentiation
Marker Expressed:Tuj1 (+)
Endoderm: In vitro spontaneous differentiation
Marker Expressed:AFP (+)
Mesoderm: In vitro spontaneous differentiation
Marker Expressed:SMA(+)
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P10
Cell line karyotype: No clinically significant imbalance was detected
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (80-84) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi021-B 66540175-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Passage number reprogrammed: P4
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: UNEWi021-A, UNEWi021-C
Characterization
Analysis of Undifferentiated Cells
Marker expression: SSEA-4(+)SSEA1(-) TRA 1-60(+)NANOG (+)
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Marker Expressed:TUBB3 (+)
Endoderm: In vitro spontaneous differentiation
Marker Expressed:AFP(+)
Mesoderm: In vitro spontaneous differentiation
Marker Expressed:ACTA2 (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P12
Cell line karyotype: No clinically significant imbalance was detected
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (80-84) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi021-C 66540176-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Passage number reprogrammed: P4
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: UNEWi021-A,UNEWi021-B
Characterization
Analysis of Undifferentiated Cells
Marker expression: NANOG(+)SSEA1(-)TRA 1-60 (+)SSEA-4(+)
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Marker Expressed:TUBB3(+)
Endoderm: In vitro spontaneous differentiation
Marker Expressed:AFP(+)
Mesoderm: In vitro spontaneous differentiation
Marker Expressed:ACTA2 (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P12
Cell line karyotype: No clinically significant imbalance was detected
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method Sendai virus description age (80-84) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену
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