Cell Lines & Specialty Cell Culture
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EmbryoMax™ KSOM Mouse Embryo Media MR-107-D
eCl@ss: 32160801 NACRES: NA.71 Legal InformationEmbryoMax is a trademark of Merck KGaA, Darmstadt, GermanyПараметрыQuality Level 300, form liquid Торговая марка EmbryoMax™, Specialty Media application(s) cell culture | embryo: suitable, cell culture | stem cell: suitable input sample type: mouse embryo(s) Узнать цену -
EmbryoMax™ KSOM Mouse Embryo Media MR-106-D
eCl@ss: 32160801 NACRES: NA.71 Storage and StabilityCan be stored at -20°C upto the expiration date on the bottle.
Once thawed it should be used within 2 weeks.
Note: Our EmbryoMax Liquid Mouse Embryo Media is produced on a bi-monthly basis. For regular users, we encourage standing orders to ensure that backorders do not occur.Legal InformationEmbryoMax is a trademark of Merck KGaA, Darmstadt, GermanyDisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 300 form liquid Торговая марка EmbryoMax™, Specialty Media application(s) cell culture | embryo: suitable, cell culture | stem cell: suitable input sample type: mouse embryo(s) Узнать цену -
EmbryoMax™ KSOM Mouse Embryo Media MR-121-D
eCl@ss: 32160801 NACRES: NA.75 Storage and StabilityCan be stored at -20°C upto the expiration date on the bottle.
Once thawed it should be used within 2 weeks.
Note: Our EmbryoMax Liquid Mouse Embryo Media is produced on a bi-monthly basis. For regular users, we encourage standing orders to ensure that backorders do not occur.Legal InformationEmbryoMax is a trademark of Merck KGaA, Darmstadt, GermanyDisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 300 form liquid Торговая марка EmbryoMax™, Specialty Media application(s) cell culture | embryo: suitable, cell culture | stem cell: suitable input sample type: mouse embryo(s) Узнать цену -
EmbryoMax™ Modified DPBS with BSA and phenol red MR-006-C
eCl@ss: 32160801 NACRES: NA.75 Storage and StabilityStore at 2 to 8°C for up to 2 months & 1 day
Note: Our EmbryoMax Liquid Mouse Embryo Media is produced on a bi-monthly basis. For regular users, we encourage standing orders to ensure that backorders do not occur.Legal InformationEmbryoMax is a trademark of Merck KGaA, Darmstadt, GermanyПараметрыQuality Level 300, form liquid Торговая марка EmbryoMax™, Specialty Media application(s) cell culture | embryo: suitable, cell culture | stem cell: suitable input sample type: mouse embryo(s) Узнать цену -
HEPES
Empirical Formula (Hill Notation): C8H18N2O4S CAS Number: 7365-45-9 Molecular Weight: 238.30 Beilstein/REAXYS Number: 883043 EC Number: 230-907-9 MDL number: MFCD00006158 PubChem Substance ID: 24895731 NACRES: NA.75
Кат. номер H6147-100G H6147-500G H6147-25G General descriptionHEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg. It is typically used in cell culture at concentration between 5mM to 30mM.ApplicationHEPES has been used:- As a constituent of modified Eagles minimum essential medium for in vitro culturing of spermatogenic cells and as a constituent of the cryoprotectant used to preserve these germ cells
- As a component of modified Hams F-10 for the incubation of doe zygotes to carry out Haploid male hemizygous recipient production
- in the preparation of 90% Percoll solution used in Percoll- gradient method for sperm collection and preparation
Packaging100, 500 g in poly bottleOther NotesThis product has been qualified for use in cell and mouse embryo culture.
Easily compare specifications for HEPES products with the HEPES specification table.Physical propertiesUseful pH range: 6.8 - 8.2ПараметрыQuality Level 400 product line BioXtra assay 99.5% (titration) form powder application(s) cell culture | embryo: suitable pH 5.0-6.5 (25 °C, 238 g/L) useful pH range 6.8 - 8.2 pKa (25 °C) 7.5, 7.5 solubility water: 500 mg/mL, clear, colorless absorption 0.05 at 290 in H2O at 33% SMILES string OCCN1CCN(CC1)CCS(O)(=O)=O InChI 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14) InChI key JKMHFZQWWAIEOD-UHFFFAOYSA-N
Safety InformationPersonal Protective Equipment Eyeshields, Gloves, type N95 (US) RIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
Hyaluronidase from bovine testes
CAS Number: 37326-33-3 Enzyme Commission (EC) Number: 3.2.1.35 ( BRENDA | IUBMB ) EC Number: 253-464-3 MDL number: MFCD00131351 NACRES: NA.75
Кат. номер H4272-PH H4272-30MG-PW H4272-30MG ApplicationHyaluronidase is an enzyme derived from bovine testes. It is used to remove the cumulus mass in the isolation of mouse embryos or from newly ovulated eggs, when preparing the eggs for in vitro culture.
Gonadotropins are used to superovulate 20 female mice. The mice are then mated and preimplantation stage embryos are retrieved and counted. The embryos are treated with hyaluronidase until the cumulus mass is dissociated from the embryos. The embryos are cultured, viewed daily and progress of the embryos is recorded.Biochem/physiol ActionsHyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression.
These enzymes randomly cleave β-N-acetylhexosamine-[1→4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.Unit DefinitionOne unit will cause a change in % transmittance at 600 nm of 0.330 per minute at pH 5.35 at 37 °C in a 2.0 mL reaction mixture (45 minute assay).Preparation NoteReconstitute 1 vial of hyaluronidase with 3 ml of M2 Medium (Product Nos. M 7167 or M 5910) or deionized water to obtain a 10 mg/ml stock solution. Dilute stock solution with M2 Medium to obtain a final working concentration of approximately 300 μg/ml.ПараметрыQuality Level 200 sterility aseptically processed type Type IV-S form powder specific activity 750-3000 units/mg solid mol wt ~55 kDa (four subunits of 14 kDa each) composition Protein, 80-105% biuret application(s) cell culture | embryo: suitable storage temp. −20°C
Safety InformationPersonal Protective Equipment Eyeshields, Gloves, type N95 (US) RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
M16 Medium MR-010-D
eCl@ss: 32160801 NACRES: NA.71 Storage and StabilityStore at -20°C for up to 2 months from date of receipt.
Note:Our EmbryoMax Liquid Mouse Embryo Media is produced on a bi-monthly basis. For regular users, we encourage standing orders to ensure that backorders do not occur.Legal InformationEmbryoMax is a trademark of Merck KGaA, Darmstadt, GermanyПараметрыQuality Level 300, form liquid Торговая марка EmbryoMax™, Specialty Media application(s) cell culture | embryo: suitable, cell culture | stem cell: suitable input sample type: mouse embryo(s) Узнать цену -
M16 Medium MR-016-D
eCl@ss: 32160801 NACRES: NA.71 Storage and StabilityStore at -20°C upto 2 months & 1 day
Frozen media can be placed in a refrigerator (2°C to 8°C) to thaw overnight. The thawed media should be swirled gently to mix the media, and warmed to 37°C prior to use. Altematively, place frozen medium into a 37°C water bath until thawed completely. It is best to gently swirl the medium during thawing to accelerate the process, and mix the medium. Do not allow the thawed medium to remain in the 37°C water bath for extended periods after it has thawed completely, and warmed to 37°C.
Once thawed the medium has a shelf life of 2 weeks, and must be stored at 2°C to 8°C between uses. Do not re-freeze the medium. Medium stored beyond this 2 week period may not perform optimally.
Note 1:
The "Last Thaw Date" on the label indicates the last day in the shelf life of the frozen media. Media stored frozen beyond this date may not perform optimally. Media are best used prior to the "Last Thaw Date" however, once the "Last Thaw Date" has been reached, the media should be thawed, stored at 2°C to 8°C, and used within 2 weeks.
Note 2:
Although the pH of these media are correct when thawed, the pH of the sodium bicarbonate buffered formulations will rise as the media are exposed to air. This rise in pH will increase with the frequency of opening the bottle, and is particularly rapid when drops of medium placed on a dish for embryo culture are exposed to air. Thus, it is recommended that the drops of medium (placed under light mineral oil) are pre-equilibrated 6 hours to overnight in a humidified incubator at 37°C, in a 5% C02 + 95% Air atmosphere, prior to introducing embryos into the medium.
Note: Our EmbryoMax Liquid Mouse Embryo Media is produced on a bi-monthly basis. For regular users, we encourage standing orders to ensure that backorders do not occur.Legal InformationEmbryoMax is a trademark of Merck KGaA, Darmstadt, GermanyDisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 300, form liquid Торговая марка EmbryoMax™, Specialty Media application(s) cell culture | embryo: suitable, cell culture | stem cell: suitable input sample type: mouse embryo(s) Узнать цену -
M16 Medium
NACRES: NA.75
Кат. номер M7292-PH M7292-50ML M7292-100ML ApplicationM2 and M16 Medium are common media for in vitro culture of preimplantation stage embryos. It is a modified Krebs-Ringer bicarbonate solution, which is very similar to Whitten′s Medium. M16 contains pyruvate and lactate as energy sources since preimplantation embryos do not utilize glucose efficiently.QualityPresence of particulates in solution will not affect product′s performance.ReconstitutionSupplement with 0.06 g/L potassium penicillin-G and 0.05 g/L streptomycin sulfate.Analysis NoteThis product is tested for its ability to support the development of one-cell mouse embryos to expanded blastocysts. Minimum requirement is 80% development to blastocyst.ПараметрыQuality Level 400 sterility sterile-filtered form liquid application(s) cell culture | embryo: suitable impurities endotoxin, tested components sodium pyruvate: 0.0363 g/L
glucose: 1.0 g/L (Dextro)
phenol red: 0.0106 g/L
NaHCO3: 2.101 g/Lshipped in ambient storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
M2 medium MR-051-F
eCl@ss: 32160801 NACRES: NA.75 Legal InformationEmbryoMax is a trademark of Merck KGaA, Darmstadt, GermanyПараметрыQuality Level 300, form liquid Торговая марка EmbryoMax™, Specialty Media application(s) cell culture | embryo: suitable, cell culture | stem cell: suitable input sample type: mouse embryo(s) Узнать цену -
M2 medium
eCl@ss: 32160801
Кат. номер MR-015-D MR-015-R ApplicationEmbryoMax M2 Medium (1X), Liquid, with Phenol RedStorage and StabilityStore at -20°C upto 2 months & 1 day
Frozen media can be placed in a refrigerator (2°C to 8°C) to thaw overnight. The thawed media should be swirled gently to mix the media, and warmed to 37°C prior to use. Altematively, place frozen medium into a 37°C water bath until thawed completely. It is best to gently swirl the medium during thawing to accelerate the process, and mix the medium. Do not allow the thawed medium to remain in the 37°C water bath for extended periods after it has thawed completely, and warmed to 37°C.
Once thawed the medium has a shelf life of 2 weeks, and must be stored at 2°C to 8°C between uses. Do not re-freeze the medium. Medium stored beyond this 2 week period may not perform optimally.
Note 1:
The "Last Thaw Date" on the label indicates the last day in the shelf life of the frozen media. Media stored frozen beyond this date may not perform optimally. Media are best used prior to the "Last Thaw Date" however, once the "Last Thaw Date" has been reached, the media should be thawed, stored at 2°C to 8°C, and used within 2 weeks.
Note 2:
Although the pH of these media are correct when thawed, the pH of the sodium bicarbonate buffered formulations will rise as the media are exposed to air. This rise in pH will increase with the frequency of opening the bottle, and is particularly rapid when drops of medium placed on a dish for embryo culture are exposed to air. Thus, it is recommended that the drops of medium (placed under light mineral oil) are pre-equilibrated 6 hours to overnight in a humidified incubator at 37°C, in a 5% C02 + 95% Air atmosphere, prior to introducing embryos into the medium.
Note: Our EmbryoMax Liquid Mouse Embryo Media is produced on a bi-monthly basis. For regular users, we encourage standing orders to ensure that backorders do not occur.Legal InformationEmbryoMax is a trademark of Merck KGaA, Darmstadt, GermanyDisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 300 form liquid Торговая марка EmbryoMax™, Specialty Media application(s) cell culture | embryo: suitable, cell culture | stem cell: suitable input sample type: mouse embryo(s) Узнать цену -
Mineral oil
CAS Number: 8042-47-5 EC Number: 232-455-8 MDL number: MFCD00131611
Кат. номер M8410-1L M8410-100ML M8410-5ML M8410-500ML M8410-BR ApplicationMineral oil has been used as an overlay in microdrop embryo cultures.Packaging1 L in glass bottle
100, 500 mL in glass bottle
5 mL in poly bottleCautionThis product is not sterile. Mineral oil is difficult to sterilize. It can only be sterilized by careful filtration of the warmed oil. It is assumed antibiotics will be added to the cell culture medium. Otherwise, Prod. No. M5310 should be used, as it is sterile filtered.Analysis NoteThis material is tested as an overlay in microdrop embryo cultures. (All embryo tested materials are tested using mouse embryos.) Typically BCF1 x BDF1 hybrids are used. The oil is primarily used to prevent evaporation of the medium, particiularly when performing embryo manipulations.1 The procedure is as follows: 1-cell (BCF1 X BDF1) embryos were cultured in triplicate microdrops of embryo-tested HTF (Human Tubal Fluid) medium supplemented with 0.4% BSA. For a valid assay, at least 70% of 1-cell stage control embryos must develop to the blastocyst stage within 96 hours. For product release, at least 80% of 1-cell stage test embryos must develop to the blastocyst stage within 96 hours with the tested material.ПараметрыQuality Level 200 sterility non-sterile product line BioReagent form light oil storage condition protect from light application(s) cell culture | embryo: suitable refractive index n20/D 1.467 (lit.) density 0.84 g/mL at 25 °C (lit.) format neat InChI key AEOVEGJBKQQFOP-DDVLFWKVSA-L
Safety InformationPersonal Protective Equipment Eyeshields, Gloves RIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point(F) No data available Flash Point(C) No data available Узнать цену -
PluriSTEM-XF® Human ES/iPS Cell Medium SCM132
eCl@ss: 32160801 NACRES: NA.75 General descriptionPluriSTEM-XF Human ES/iPS Medium is a complete xeno-free medium formulation for the feeder-free culture of human ES and iPS cells. Pluripotent cells maintained in thissmall molecule based medium exhibit high cell health, express high levels of pluripotency markers (NANOG, OCT3/4, SOX-2, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81), retain differentiation potential and possess a normal karyotype. PluriSTEM-XF Human ES/iPS Medium is provided as a stand-alone 500 mL bottle that is ready-to-use and does not require additional supplementation.
For a complete xeno-free culture system, PluriSTEM-XF Human ES/iPS Medium should be used with PluriSTEM-XF Recombinant Vitronectin (Cat. No. CC130).
Each lot of PluriSTEM-XF Human ES/iPS Medium is rigorously quality control tested for the ability to sustain undifferentiated human ES/iPS cells for 3 passages.
Note: Before starting PluriSTEM-XF, users are recommended to read the manual carefully and completely. Colony morphology and growth rates may differ from other serum-free, feeder-free media culture conditions.ApplicationResearch Category
Stem Cell Research
Research Sub Category
Pluripotent & Early DifferentiationQualityEach lot of PluriSTEM® Human ES/iPS Medium is rigorously quality control tested for the ability to sustain undifferentiated human ES cells for greater than 3 passages.Storage and StabilityPluriSTEM-XF Human ES/iPS Medium is provided frozen and can be stored at -20°C until the expiration date on the product label.
Before use, thaw PluriSTEM-XF medium at room temperature (15 – 25°C) or overnight at 2 – 8°C. Do not thaw at 37°C. Upon thawing, PluriSTEM-XF medium may be aseptically dispensed into working aliquots and stored at -20°C until the expiration date as indicated on the label. Thawed aliquots may be stored at 2 – 8°C for up to 2 weeks. Do not refreeze aliquots after thawing. Before use, warm working aliquots to room temperature. Do not warm the medium in a 37°C water bath.Legal InformationPLURISTEM is a registered trademark of Merck KGaA, Darmstadt, GermanyDisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 100, form liquid Торговая марка PluriSTEM® application(s) cell culture | embryo: suitable, cell culture | stem cell: suitable shipped in dry ice
Safety InformationWGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PluriSTEM® Human ES/iPS Cell Medium SCM130
eCl@ss: 32160801 NACRES: NA.75 General descriptionEMD Millipore has developed a complete, defined and serum-free medium formulation for the feeder-free culture of human ES and iPS cells. PluriSTEM Human ES/iPS Cell (Cat. No. SCM130) is a small molecule based medium that enables weekend-free culture of human pluripotent stem cells and allows for media exchanges every other day without compromising the morphology or long term functionality of pluripotent stem cells. Pluripotent cells maintained in this moderate feeding regiment exhibited high cell health with minimal spontaneous differentiation,; expressed high levels of pluripotency markers (NANOG, OCT3/4, SOX-2, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81), retained differentiation potential and possessed a normal karyotype. PluriSTEM Human ES/iPS Medium is provided as a stand-alone 500 mL bottle that is ready-to-use and does not require additional supplementation. Cells proliferate faster in PluriSTEM as compared to the same cells plated in other serum-free and feeder-free media systems; a typical 4-6 day passage is expected for most pluripotent cell lines.
PluriSTEM has been extensively tested and proven to possess the following characteristics for Human ES/iPS cell culture:
• Supports the culture of pluripotent human ES/iPS cells for greater than 30 passages.
• Eliminates the requirement to feed cells over the weekend. Take the weekend off.
• Eliminates the requirement of daily feeding (i.e. Monday, Wednesday, Friday media exchanges).
• Simple and easy transition of cells to PluriSTEM from feeder-based and feeder-free culture systems.
• Enables superior single cell culture, passage and selection of human pluripotent clones.
• Superior freeze-thaw cell viability and recovery.
• Supports suspension culture.
View our Application Note! (See the Data!,)QualityEach lot of PluriSTEM® Human ES/iPS Medium is rigorously quality control tested for the ability to sustain undifferentiated human ES cells for greater than 3 passages.
pH= 7.2-7.4
Osmolality= 315-345
Appearance= Red Clear LiquidStorage and StabilityPluriSTEM Human ES/iPS Medium is provided frozen and can be stored at -20°C until the expiration date on product label. Before use, thaw PluriSTEM at room temperature or overnight at 2-8°C. Do not thaw at 37°C. If desired, PluriSTEM may be aseptically dispensed into working aliquots and stored at -20°C. Use aliquots within expiration date as indicated on label. Thawed aliquots may be stored at 2 – 8°C for up to 2 weeks. Do not refreeze aliquots after thawing. Before use, warm working aliquots to room temperature. Do not warm the medium in a 37°C water bath.Legal InformationPLURISTEM is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыQuality Level 100, form liquid Торговая марка PluriSTEM® application(s) cell culture | embryo: suitable, cell culture | stem cell: suitable shipped in dry ice
Safety InformationWGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Polyvinylpyrrolidone
Linear Formula: (C6H9NO)n CAS Number: 9003-39-8 MDL number: MFCD00149016 PubChem Substance ID: 24898139 NACRES: NA.75
Кат. номер P0930-50G P0930-100G General descriptionPolyvinylpyrrolidone (PVP)/polyvidone is used in cosmetics, medical and hair care products. Povidone iodine, a compound of polyvinylpyrrolidone and iodine can be used as an antiseptic and antibacterial agent. Dry form of PVP is available as a light flaky hygroscopic powder, which can absorb up to 40% of water by its weight.ApplicationPolyvinylpyrrolidone has been used as a component in phosphate buffer saline (PBS) for immunostaining. It has also been used as a component in phosphate buffer saline (PBS) to wash parthenogenetic embryos for immunocytochemical analysis.Packaging50, 100 g in poly bottleOther NotesPolyvinylpyrrolidone is a component of Denhardt′s Solution and is included at a concentration of 1% (w/v) in the standard 50X stock solution.ПараметрыQuality Level 200 product line BioXtra form powder application(s) cell culture | embryo: suitable viscosity number 28-32(lit.) solubility H2O: 100 mg/mL Featured Industry Agriculture InChI 1S/C6H9NO/c1-2-7-5-3-4-6(7)8/h2H,1,3-5H2 InChI key WHNWPMSKXPGLAX-UHFFFAOYSA-N
Safety InformationPersonal Protective Equipment Eyeshields, Gloves, type N95 (US) RIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
Protease from Streptomyces griseus
CAS Number: 9036-06-0 Enzyme Commission (EC) Number: 3.4.24.31 ( BRENDA | IUBMB ) EC Number: 232-909-5 MDL number: MFCD00132092 eCl@ss: 32160410 NACRES: NA.75
Кат. номер P8811-100MG P8811-5G P8811-1G SpecificityA mixture of at least three proteolytic activities including an extracellular serine protease. In general, serine proteases display a wide range of substrate specificities, which are believed to be mediated by an active site composed of one Asp, one His, and a Ser residue in the molecule. This enzyme prefers to hydrolyze peptide bonds on the carboxyl side of glutamic or aspartic acid.ApplicationThis enzyme is more active at a higher pH range than the known alkaline protease, showing the proteolytic activity even in 0.2N NaOH solution. This enzyme is useful for proteolysis of insoluble protein and for structure investigation of protein.
Protease is typically used in nucleic acid isolation procedures in incubations of 0.5-3.0 hours supplemented with 0.2% sodium dodecyl sulfate and 10 mM EDTA.Packaging100 mg in glass bottle
1, 5 g in glass bottleOther NotesAn unusually non-specific protease.QualityContains approximately 25% calcium acetate.Physical propertiesCompletely inactivated by heating above 80 °C for 15-20 minutes.Unit DefinitionOne unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).Preparation NoteCollected from culture broth of S. griseus.ПараметрыQuality Level 200 product line BioReagent form powder specific activity 3.5 units/mg solid application(s) cell culture | embryo: suitable impurities starch, essentially free storage temp. 20°C
Safety InformationPictograms GHS07,GHS08 Signal Word Danger Hazard Statements H315 - H319 - H334 - H335 Precautionary Statements P302 + P352 - P305 + P351 + P338 Target Organs Respiratory system Personal Protective Equipment Eyeshields, Gloves, type N95 (US) RIDADR NONH for all modes of transport WGK Germany WGK 2 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
Sodium DL-lactate solution L7900-100ML
Empirical Formula (Hill Notation): C3H5NaO3 Linear Formula: CH3CH(OH)COONa CAS Number: 72-17-3 Molecular Weight: 112.06 Beilstein/REAXYS Number: 4332999 EC Number: 200-772-0 MDL number: MFCD00065400 PubChem Substance ID: 24896486 NACRES: NA.75 ApplicationLactic acid is an α hydroxyl carboxylic acid produced from pyruvate by the enzyme lactate dehydrogenase (LDH). This mixed isomer lactate has been qualified for use in mouse embryo culture. Lactate regulates pyruvate uptake and metabolism in the preimplantation mouse embryo.ПараметрыQuality Level 200 biological source synthetic product line BioXtra form syrup concentration 60 % (w/w) application(s) cell culture | embryo: suitable storage temp. 2-8°C SMILES string [Na+].CC(O)C([O-])=O InChI 1S/C3H6O3.Na/c1-2(4)3(5)6;/h2,4H,1H3,(H,5,6);/q;+1/p-1 InChI key NGSFWBMYFKHRBD-UHFFFAOYSA-M
Safety InformationPersonal Protective Equipment Eyeshields, Gloves, type N95 (US) RIDADR NONH for all modes of transport WGK Germany WGK 2 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
Sodium pyruvate
Empirical Formula (Hill Notation): C3H3NaO3 Linear Formula: CH3COCOONa CAS Number: 113-24-6 Molecular Weight: 110.04 Beilstein/REAXYS Number: 3568341 EC Number: 204-024-4 MDL number: MFCD00002586 PubChem Substance ID: 24898552 NACRES: NA.75
Кат. номер P4562-100G P4562-5G P4562-25G ApplicationSodium pyruvate is used by cells as an easily accessible carbohydrate source. Additionally, it is involved with amino acid metabolism and initiates the Kreb′s cycle. Sodium pyruvate attenuates oxidative stress in vitro. The 100 mM solution should be diluted 1:100 for most cell culture applications. This product has been qualified for use in mouse embryo cultures.Packaging5, 25, 100 g in poly bottleПараметрыQuality Level 200 product line BioXtra assay 99% form powder application(s) cell culture | embryo: suitable mp >300 °C (lit.) solubility H2O: 100 mg/mL storage temp. 2-8°C SMILES string [Na+].CC(=O)C([O-])=O InChI 1S/C3H4O3.Na/c1-2(4)3(5)6;/h1H3,(H,5,6);/q;+1/p-1 InChI key DAEPDZWVDSPTHF-UHFFFAOYSA-M
Safety InformationPictograms GHS07 Signal Word Warning Hazard Statements H317 - H319 Precautionary Statements P280 - P302 + P352 - P305 + P351 + P338 Personal Protective Equipment Eyeshields, Gloves, type N95 (US) RIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
Tyrodes Solution, Acidic T1788-100ML
NACRES: NA.75 ApplicationFor removal of the zona pellucida.Параметрыsterility sterile-filtered Quality Level 400 form liquid application(s) cell culture | embryo: suitable impurities endotoxin, tested pH 2.5±0.3 shipped in dry ice storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany nwg Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
Water
Empirical Formula (Hill Notation): H2O Linear Formula: H2O CAS Number: 7732-18-5 Molecular Weight: 18.02 Beilstein/REAXYS Number: 2050024 EC Number: 231-791-2 MDL number: MFCD00011332 PubChem Substance ID: 24900778 NACRES: NA.75
Кат. номер W1503-500ML W1503-100ML ApplicationFor use in embryo manipulation.
Water was used for the following applications:- Used in toxicological analyses for the suspension of emission particles
- Used for suspension of particulate samples in various studies
- Used in cell cultivation and exposure
- Used for washing cells in calcium free water during the detection of intracellular calcium changes
Other NotesEasily compare specifications for water products with the water specification table.ПараметрыQuality Level 200 vapor density <1 (vs air) vapor pressure 3 mmHg sterility sterile-filtered product line BioXtra form liquid application(s) cell culture | embryo: suitable impurities endotoxin, tested refractive index n20/D 1.34 (lit.) pH 5-7 bp 100 °C (lit.) mp 0 °C (lit.) density 1.000 g/mL at 3.98 °C (lit.) SMILES string O InChI 1S/H2O/h1H2 InChI key XLYOFNOQVPJJNP-UHFFFAOYSA-N
Safety InformationPersonal Protective Equipment Eyeshields, Gloves RIDADR NONH for all modes of transport WGK Germany nwg Flash Point(F) No data available Flash Point(C) No data available Узнать цену -
Bovine Aortic Endothelial Cells: BAOEC (Cryovial) B304-05
NACRES: NA.81 General descriptionBovine large artery endothelial cells (BAOEC) provide an excellent model system to study all aspects of cardiovascular function and disease, such as critical signaling pathways and mechanisms relevant to proper function of endothelia, including angiogenesis, permeability, flow adaptation, NO production, diabetes-related complications, etc., and search for beneficial modulators and delivery systems for therapeutic use, and to design scaffolds, surfaces and materials for tissue engineering and 3D modeling.Cell Line OriginAortaApplicationcardiovascular function, studies on immune system and graft rejection, development of 3D endothelialized engineered tissues, drug discovery, stent-graft compatibility testingComponentsBovine Endothelial Cell Growth Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Bovine Endothelial Cell Growth Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 16 doublings
Subculture RoutinePlease refer to the BAOEC Culture Protocol.Параметрыbiological source bovine aorta (USDA-inspected cattle) packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 60 morphology Endothelial application(s) cell culture | mammalian: suitable relevant disease(s) diabetes; cardiovascular diseases shipped in dry ice storage temp. −196°C
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Canine Aortic Endothelial Cells: CnAOEC (Cryovial) CN304-05
NACRES: NA.81 General descriptionCnAOEC provide a useful model to study cardiovascular diseases and test potential therapeutic agents, not only important from a veterinary perspective, but also having implications for human health. In a study aiming to adapt an immunomagnetic isolation protocol used in human patients to isolate circulating endothelial cells in dogs, CnAOEC cells were shown to be positive for von Willebrand factor, CD146 and stained with Ulex europaeus agglutinin 1 (Wills, 2009). To investigate the mechanism of N-terminal portion of pro C-type natriuretic peptide (NT-pCNP), a sepsis biomarker in humans and dogs, CnAOEC were treated with a variety of agents and it was found that lipopolysaccharide, TNF-α and IL-1β, but not IL-6, IL-10, IL-21, CXCL-8, IFN-γ, VEGF-A, stimulated NT-pCNP production (Osterbur, 2012, 2013). CnAOEC were also used to demonstrate vasoprotective activity of pomegranate and soy isoflavons in order to prevent endothelial disfunction in dogs which commonly leads to cardiovascular disease (Baumgartner-Parzer, 2012). Finally, CnAOEC were utilized as a control to compare the growth of normal canine endothelial cells and aggressive canine hemangiosarcoma cells in a study investigating signaling pathways underlying hemangiosarcoma oncogenesis (Murai, 2012), and as a VEGFR2-positive control in a study aimed at understanding the reasons for reduced numbers of circulating progenitor cells in cardiovascular disease (Boilson, 2010).Cell Line OriginAortaApplicationcardiovascular function, studies on immune system and graft rejection, development of 3D endothelialized engineered tissues, drug discovery, stent-graft compatibility testingComponentsCanine Endothelial Cell Basal Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Canine Endothelial Cell Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 16 doublings
Subculture RoutinePlease refer to the CnAOEC Culture Protocol.Параметрыbiological source canine aorta packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 78 morphology Endothelial application(s) cell culture | mammalian: suitable relevant disease(s) diabetes; cardiovascular diseases shipped in dry ice storage temp. −196°C
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Canine Osteoblasts: CnOb (Cryovial) CN406-05
NACRES: NA.81 General descriptionCanine osteoblasts provide an excellent model system for studies relevant to the skeletal system, including mechanisms of cancerogenesis.
Canine osteoblasts have been used to define a biomarker profile of canine osteosarcoma (Milovancev, 2013), elucidate the roles of oncogenic STAT3 (Fossey, 2009, 2011) and canonical Wnt signaling (Piskun, 2013) pathways, and to develop personalized osteosarcoma treatments by testing Src inhibitor dasatinib (Davis, 2013), procaspase-3 activating compound PAC-1 (Book, 2012), HSP90 inhibitor STA-1474 (McCleese, 2009) and STAT3 inhibitor LLL12 (Couto, 2012, 2013).
Characterization: Positive for bone mineralization.Cell Line OriginBoneApplicationmodel for skeletal system research, signaling pathways, bone morphogenesis, gene expression, cell activation and proliferation, wound healing, bone formation and regeneration, treatment strategies, tissue engineering, in vitro bone models, mineralization, hormone treatmentsComponentsCanine Osteoblast Basal Medium containing 10% FBS & 10% DMSOPreparation Note- 1st passage, >500,000 cells in Canine Osteoblast Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 10 doublings
Subculture RoutinePlease refer to the CnOb Culture Protocol.Параметрыbiological source canine bone (normal) packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 78 morphology osteoblast application(s) cell culture | mammalian: suitable relevant disease(s) arthritis; osteoporosis shipped in dry ice storage temp. −196°C
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Canine Skeletal Muscle Cells: CnSkMC (Cryovial) CN150-05
NACRES: NA.81 General descriptionCanine Skeletal Muscle Cells (CnSkMC) provide a useful system to study many aspects of muscular function and disease and can serve as an intermediate model of human diseases. Skeletal Muscle Cells also play an instrumental role in the glucose metabolism and diabetes.Cell Line OriginMuscleApplicationlipid and glucose metabolism, exercise, insulin action, cell signalingComponentsCanine Skeletal Muscle Cell Basal Medium containing 10% FBS & 10% DMSOPreparation Note- 1st passage, >500,000 cells in Canine Skeletal Muscle Cell Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 15 doublings
Subculture RoutinePlease refer to the CnSkMC Culture Protocol.Параметрыbiological source canine skeletal muscle (normal limb) packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 78 morphology Skeletal muscle application(s) cell culture | mammalian: suitable relevant disease(s) muscular dystrophy shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
Hair Follicle Dermal Papilla Cells: HFDPC, adult 602-05A
NACRES: NA.81 General descriptionHuman Hair Follicle Dermal Papilla Cells (HFDPC) are mesenchymal cells isolated from the hair papilla of normal human scalp hair follicles. Hair papilla, in the adult hair follicle, plays a crucial role in the dermal-epidermal interactions that control hair production and events of the hair growth cycle. HFDPC can therefore be used for studying cellular mechanisms organizing the hair follicle, and for the development and evaluation of hair growth products.
HFDPC have been used to demonstrate that IL-1 affects dermal papilla activity by decreasing androgen receptor expression and stimulating secretion of KFG, VEGF, IL-8 and GM-CSF (Boivin, 2006). They were also used to investigate how gene expression and cell proliferation are affected by testosterone analogues (Reiter, 2008), potential hair growth enhancing agents (Sasajima, 2008) and microbial metabolites (Takigawa, 2012). Using HFDPC, along with Human Epidermal Keratinocytes and Human Bronchial Epithelial Cells, scientists also studied mechanisms of cellular senescence, and develop ed methodology to extend cellular life span and immortalize the cells by inhibiting p16INK4a and introducing hTERT (Haga, 2007). Finally, HFDPC have been used to discover the link between the androgen-induced alopecia and circadian rhythms, mediated by a “clock gene” transcription factor BMAL1 (Watabe, 2013).Cell Line OriginScalpApplicationdermatalogical interactions, hair production, induction and maintenance of hair growth, hair follicle organization, development and evaluation of hair growth products, effects and secretion of growth factors and cytokines, gene expression, cell proliferation, cell senescence, cell lifespan extension, in vitro screening of androgen blocking agents, reservoir of multi-potent stem cells, skin reconstruction, wound healing, signal transduction pathways, skin composites and substitutes, hair follicle neogenesisComponentsCell Basal Medium containing 20% FBS & 5% DMSOPreparation Note- 2nd passage, >500,000 cells in Cell Basal Medium containing 20% FBS & 5% DMSO
- Can be cultured at least 6 doublings
Subculture RoutinePlease refer to the HFDPC Culture Protocol.Параметрыbiological source human scalp (hair follicle papilla) packaging pkg of 500,000 cells growth mode Adherent morphology mesenchymal application(s) cell culture | mammalian: suitable relevant disease(s) Alzheimer′s disease shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
Human Aortic Endothelial Cells: HAOEC, adult 304-05A
NACRES: NA.81 General descriptionHuman Aortic Endothelial Cells (HAOEC) provide an excellent model system to study all aspects of cardiovascular function and disease, and they have been utilized in dozens of research publications to study diabetes-associated complications related to cardiovascular function, investigate mechanisms of immune response and graft rejection, study endothelial dysfunction caused by air pollution, oxidative stress and inflammation, and develop 3d endothelialized engineered tissues, as well as new technologies based on novel material surfaces and drugs in order to reduce risks associated with vascular implants.
Select HAOEC lots have been additionally tested to demonstrate stimulation-dependent angiogenesis and key endothelial cell signaling pathways (phosphorylation of VEGFR, Akt, MAPK, and expression of Tie2, eNOS, Axl and Etk/Bmx).
HAOEC from Cell Applications, Inc. have been used to:- demonstrate that increased glucose flux leads to endothelial dysfunction in diabetes via activating Egr1-mediated proinflammatory and prothrombotic responses (Vedantham, 2013);
- study apoptosis, oxidative stress and inflammation associated with atherosclerosis (Fu, 2014) and demonstrate the beneficial effects of anthocyanin on endothelial cells damaged by exposure to oxidized sterols (Wang, 2012);
- demonstrate that upregulation of thioredoxin via AMPK-FOXO3 pathway protects endothelial cells from oxidative stress and may prevent cardiovascular diseases in patients with metabolic syndrome and diabetes (Li, 2009a,b; Hou, 2010) and further elucidate the involvement of AMPK cascade in mediating beneficial cardiovascular effects of green tea (Reiter, 2010);
- test anti-inflammatory and vasodilating properties of a synthetic rutaecarpine derivative (Lee, 2013);
- show that glycated albumin, associated with diabetic complications, decreases endothelial miR-146a expression which leads to increased IL-6 production, and that angiotensin protects endothelial cells by preventing miR-146a downregulation (Wang, 2013);
- demonstrate that air pollutants can directly affect ZO-1 function leading to increased endothelial permeability, inflammatory cell transmigration and initiation of atherosclerosis (Li, 2010);
- discover the involvement of stress signaling JNK and p38 pathways in pathological suppression of thrombomodulin, a vascular protective molecule, downregulated in many thrombotic and vascular diseases (Rong, 2010);
- link uremic toxins (in particular, PAA) in patients with chronic liver disease to increased ROS production and stimulation of TNF-a in endothelial cells leading to atherosclerosis and vascular calcification (Morita, 2011);
- demonstrate that in diabetes, advanced glycation end products lead to ROS generation in endothelia via sustained NF-kB activation, contributing to progression of atherosclerosis (Morita, 2013);
- discover that CD40 ligand promotes monocyte adhesion to endothelial cells via PKCa, NF-kB and VCAM-1 signaling cascade, explaining the role of CD40L in atherogenesis (Wu, 2013);
- show that monocytes activated by endothelial cells, produce CD80 signaling that leads to allogenic immune response, indicating the need for specific therapy to prevent monocyte activation during allograft transplantation (Wang, 2008);
- identify tetraspanin CD82 as the recognition sensor responsible for rejection of xenotransplants (Saleh, 2013);
- develop 3d endothelialized engineered tissues (Sakai, 2012), as well as new technology based on novel material surfaces and drugs (such as paclitaxel, sirolimus, vitamin C, C6-ceramide and 17?-estradiol) to inhibit smooth muscle cell proliferation at the same time allowing endothelial cells adhesion and proliferation in order to reduce risk associated with vascular implants (Wang, 2009, 2011; Kanie, 2012; Deshpande, 2013; Kakade, 2013; Lamichane, 2013);
Additionally, HAOEC, along with human subclavian artery (HScAEC), carotid artery (HCtAEC), coronary artery (HCAEC) and brachiocephalic artery (HBcAEC) have been used to demonstrate that not only blood vessels from different tissues are highly heterogeneous, they also interact differently with leukocytes during the inflammation response (Scott, 2013). The authors further showed that differential N-glycosylation of commonly expressed vascular adhesion molecules may be responsible for this heterogeneity, as well as for modulation of signaling under resting and activated inflammatory conditions. This also explains why specific vascular beds may be more or less susceptible to particular diseases or stimuli. Importantly, if cells from different sources were used, these results could not be convincingly validated due to a number of uncontrolled variables, such as age, race, genetic variability or life style choices of the donors.
Because of the complex heterogeneity that exists not only between different donors, but even between different vascular beds in the same individual, it would be prudent to confirm any new findings on primary cell lots coming from several different origins.Cell Line OriginAortaApplicationcardiovascular function, studies on immune system and graft rejection, development of 3D endothelialized engineered tissues, drug discovery, stent-graft compatibility testingComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 16 doublings
Subculture RoutinePlease refer to the HAOEC Culture Protocol.ПараметрыQuality Level 100 biological source human aorta packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology Endothelial application(s) cell culture | mammalian: suitable relevant disease(s) diabetes; cardiovascular diseases shipped in dry ice storage temp. −196°C
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Human Aortic Endothelial Cells: HAOEC: Pre-Screened for Angiogenesis & VEGF signaling, adult S304-05A
NACRES: NA.81 General descriptionEndothelial cells (EC) line the blood vessels of the entire circulatory system, from aorta to capillaries, and act as a barrier between circulating blood and the rest of the vessel wall/surrounding tissues. Endothelial cells are involved in the processes of angiogenesis, vasculogenesis, vasoregulation, coagulation, fibrinolysis and selective blood barrier. They are also implicated in pathophysiological processes, including cardiovascular disease and cancer development.
Matched smooth muscle cells obtained from the same arteries are available as well.The most commonly used human endothelial cells are Human Umbilical Vein Endothelial Cells, (HUVEC), Human Microvascular Endothelial Cells (HMVEC) and Human Aortic Endothelial Cells (HAOEC). Select lots of HUVEC, HMVEC, and HAOEC have been pre-screened to demonstrate stimulation-dependent angiogenesis and key endothelial cell signaling pathways (phosphorylation of VEGFR, Akt, MAPK, and expression of Tie2, eNOS, Axl and Etk/Bmx.
VEGF-Stimulated Signaling in Pre-Screened Endothelial Cells
VEGF receptor-2 (VEGFR-2) is a major VEGF of endothelial cells. VEGFR-2-mediated signaling plays a critical role in angiogenesis, including regulation of proliferation, differentiation, cell movement, and survival of endothelial cells. VEGF-induced receptor dimerization triggers activation of VEGFR-2 tyrosine kinase and autophosphorylation at a specific set of tyrosine residues, which serve as docking sites for downstream signaling components leading to activation of downstream signaling molecules, including phosphorylation of Akt and p44/42-MAPK.
Expression of Signaling Biomarkers in Pre-Screened Endothelial Cells
Prescreened Endothelial Cells express the following important biomarkers:- Tie2, a receptor tyrosine kinase critical for the angiogenic remodeling, sprout formation, survival of endothelial cells and vessel stabilization processes;
- eNOS (endothelial Nitric Oxide Synthase), the enzyme that producse NO which is an important signaling molecule that regulates a diverse range of physiological events and is required for normal endothelial function;
- Axl, a receptor tyrosine kinase whose ligand is the survival factor Gas6 (growth arrest-specific gene 6 product) implicated in many processes, such as cell survival, leukocyte transmigration and neointima formation.
- Etk/Bmx (Endothelial/epithelial Tyrosine Kinase), a member of the Btk family, participates in signal transduction stimulated by growth factor receptors, cytokine receptors, G-protein-coupled receptors, antigen receptors, and integrins, and has been implicated in cell adhesion, migration, proliferation, and survival.
Cell Line OriginBlood VesselsApplicationcardiovascular function, studies on immune system and graft rejection, development of 3D endothelialized engineered tissues, drug discovery, stent-graft compatibility testingComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 16 doublings
Subculture RoutinePlease refer to the HAOEC Culture Protocol.ПараметрыQuality Level 100 biological source human blood vessels packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology Endothelial application(s) cell culture | mammalian: suitable relevant disease(s) diabetes; cardiovascular diseases shipped in dry ice storage temp. −196°C
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Human Aortic Smooth Muscle Cells: HAOSMC, adult 354-05A
NACRES: NA.81 General descriptionHuman Aortic Smooth Muscle Cells (HAOSMC) provide an excellent model system to study all aspects of cardiovascular function and disease, especially those related to mechanisms of hyperplasia and hypertrophy of intimal smooth muscle cells leading to vascular occlusion in atherosclerosis and stent restenosis.
HAOSMC have been utilized in dozens of research studies, for example, to:elucidate cytokines and growth factors signaling pathways implicated in the molecular regulation of smooth muscle cell proliferation, migration, and overall vascular function (Liu, 2009; Tan, 2009; Seymour, 2010; Chen, 2011; Hirase, 2013; Stein, 2013):- Understand hyperglycemia-related risk factors for atherosclerosis in diabetes patients (Maier, 2010), as well as effects of ethanol on vascular calcification (Oros, 2012)
- Investigate extracellular elastic matrix deposition and its role in cardiovascular health, repair of damaged vasculature and successful tissue engineering (Gacchina, 2010, 2011; Venkataraman, 2012; Phillippi, 2013)
- Study mechanisms and effects of mechanoregulation on proliferation and function of smooth muscle cells (Jiang, 2009; Venkataraman, 2013, 2014), and develop advanced stent technology, including novel surface materials, and drug and gene delivery systems to prevent restenosis and other occlusive vasculopathies (Brito, 2010; Deshpande, 2013; Kakade, 2013; Lamichhane, 2013)
Characterization: Positive for smooth muscle cell specific alpha-actin expression.Cell Line OriginAortaApplicationaortic wall contraction, role of smc under normal conditions, blood pressure, distribution of oxygenated blood through systemic circulationComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 16 doublings
Subculture RoutinePlease refer to the HAOSMC Culture Protocol.Параметрыbiological source human aorta (normal, tunica intima and tunica media, fibrous plaque-free) Quality Level 100 packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology smooth muscle application(s) cell culture | mammalian: suitable relevant disease(s) cardiovascular diseases shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
Human Aortic Smooth Muscle Cells: HAOSMC, fetal 354-05F
NACRES: NA.81 General descriptionHuman Aortic Smooth Muscle Cells (HAOSMC) provide an excellent model system to study all aspects of cardiovascular function and disease, especially those related to mechanisms of hyperplasia and hypertrophy of intimal smooth muscle cells leading to vascular occlusion in atherosclerosis and stent restenosis.
HAOSMC have been utilized in dozens of research studies, for example, to:elucidate cytokines and growth factors signaling pathways implicated in the molecular regulation of smooth muscle cell proliferation, migration, and overall vascular function (Liu, 2009; Tan, 2009; Seymour, 2010; Chen, 2011; Hirase, 2013; Stein, 2013);- understand hyperglycemia-related risk factors for atherosclerosis in diabetes patients (Maier, 2010), as well as effects of ethanol on vascular calcification (Oros, 2012);
- investigate extracellular elastic matrix deposition and its role in cardiovascular health, repair of damaged vasculature and successful tissue engineering (Gacchina, 2010, 2011; Venkataraman, 2012; Phillippi, 2013);
- study mechanisms and effects of mechanoregulation on proliferation and function of smooth muscle cells (Jiang, 2009; Venkataraman, 2013, 2014), and develop advanced stent technology, including novel surface materials, and drug and gene delivery systems to prevent restenosis and other occlusive vasculopathies (Brito, 2010; Deshpande, 2013; Kakade, 2013; Lamichhane, 2013).
Characterization: Positive for smooth muscle cell specific alpha-actin expression.Cell Line OriginAortaApplicationaortic wall contraction, role of smc under normal conditions, blood pressure, distribution of oxygenated blood through systemic circulationComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 3rd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 16 doublings
Subculture RoutinePlease refer to the HAOSMC Culture Protocol.Параметрыbiological source human aorta (normal, tunica intima and tunica media, fibrous plaque-free) Quality Level 100 packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology smooth muscle application(s) cell culture | mammalian: suitable relevant disease(s) cardiovascular diseases shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
Human Brachiocephalic Artery Endothelial Cells: HBcAEC, adult 3012-05A
NACRES: NA.81 General descriptionHBcAEC, along with human aortic (HAOEC), carotid artery (HCtAEC), coronary artery (HCAEC) and subclavian artery (HScAEC) have been used to demonstrate that not only blood vessels from different tissues are highly heterogeneous, they also interact differently with leukocytes during the inflammation response (Scott, 2013). The authors further showed that differential N-glycosylation of commonly expressed vascular adhesion molecules may be responsible for this heterogeneity, as well as for modulation of signaling under resting and activated inflammatory conditions. This also explains why specific vascular beds may be more or less susceptible to particular diseases or stimuli. Importantly, if cells from different sources were used, these results could not be convincingly validated due to a number of uncontrolled variables, such as age, race, genetic variability or life style choices of the donors.
Because of the complex heterogeneity that exists not only between different donors, but even between different vascular beds in the same individual, it would be prudent to confirm any new findings on primary cell lots coming from several different origins.Cell Line OriginArteryApplicationoxygenated blood supply, endothelial permeability, blood albumin leakage, endothelial integrityComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 12 doublings
Subculture RoutinePlease refer to the HBcAEC Culture Protocol.ПараметрыQuality Level 100 biological source human brachiocephalic artery (normal) packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology Endothelial application(s) cell culture | mammalian: suitable relevant disease(s) cardiovascular diseases shipped in dry ice storage temp. −196°C
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Human Brachiocephalic Artery Smooth Muscle Cells: HBcASMC, adult 3512-05A
NACRES: NA.81 General descriptionHuman Brachiocephalic Artery Smooth Muscle Cells (HBcASMC) provide a useful in vitro system to study all aspects of cardiovascular function and disease, especially those related to mechanisms of hyperplasia and hypertrophy of intimal smooth muscle cells leading to vascular occlusion in atherosclerosis and stent restenosis. Characterization: positive for smooth muscle cell specific alpha-actin expression.Cell Line OriginArteryApplicationcardiovascluar function, oxygenated blood supply to arm, head, neck, blood pressure, artery flexibilityComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 12 doublings
Subculture RoutinePlease refer to the HBcASMC Culture Protocol.ПараметрыQuality Level 100 biological source human brachiocephalic artery (normal) packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology smooth muscle application(s) cell culture | mammalian: suitable relevant disease(s) diabetes; cardiovascular diseases shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
Human Bronchial Epithelial Cells: HBEpC, adult 502-05A
NACRES: NA.81 General descriptionHBEpC provide an excellent model system to study all aspects of epithelial function and disease, particularly those related to airway viral infections, as well as tissue repair mechanisms, signaling changes and potential treatments relevant to lung injuries, mechanical and oxidative stress, inflammation, pulmonary diseases and smoking.
When grown on inserts and provided with the liquid/air interface, HBEpC can differentiate into a pseudostriated epithelium and serve as a more physiological 3D tissue model for in vitro studies.Cell Line OriginBronchiApplicationepithelial function, tissue repair, cell signaling, lung injury treatment, mechanical and oxidative stress tests, cell differentiation, 3D tissue model, cytokine and growth factor production, apoptosis, cell adhesion molecules, cell permeability, pro-inflammatory signaling, viral fusion mechanisms, gene expression, library screening, mucin secretionComponentsCell Basal Medium containing 10% FBS & 10% DMSOPreparation Note- 1st passage, >500,000 cells in Cell Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 16 doublings
Subculture RoutinePlease refer to the HBEpC Culture Protocol.ПараметрыQuality Level 100 biological source human bronchi (normal surface epithelium) packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology epithelial application(s) cell culture | mammalian: suitable relevant disease(s) asthma; cardiovascular diseases; autoimmune diseases shipped in dry ice storage temp. −196°C
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Human Cardiac Fibroblasts: HCF, adult 306-05A
NACRES: NA.81 General descriptionCardiac fibroblasts are the most prevalent cell type in the heart, making up 60-70 % of all cells. HCF from Cell Applications, Inc. provide an excellent model system to study many aspects of human heart function and pathophysiology.
HCF has been utilized in a number of research publications, for example to:- Determine that electrical coupling between cardiomyocytes and fibroblasts is mediated by large-conductance Ca2+-activated K+ channels that can be stimulated by estrogen receptor agonists (Wang, 2006, 2007); and show that antimitogenic effects of estradiol on HCF growth are mediated by cytochromes 1A1/1B1-and catechol-O-methyltransferase-derived metabolites (Dubey, 2005)
- Show that in response to mechanical stretch, cardiac fibroblasts release TGF-b which causes trombomodulin downregulation, increasing the risk of thromboembolic events (Kapur, 2007) and also induces cardiac fibroblast differentiation into myofibroblasts via increased Smad2 and ERK1/2 phosphorylation, that could be stimulated by endothelin-1 and inhibited by Ac-SDKP (Peng, 2010)
- Demonstrate that activation of G protein-coupled receptor kinase-2 (GRK2) prevents normal regulation of collagen synthesis in cardiac fibroblasts mimicking heart failure phenotype (DSouza, 2011); identify FGF2 signaling pathway as potential target for modulating apoptosis in cardiac pathology (Ma, 2011) and investigate the roles of scleraxis (Bagchi, 2012) and AMPKa1 (Noppe, 2014) in scar formation following myocardial infarction
- Show that the KATP channel opener KMUP-3 preserved cardiac function after myocardial infarction by enhancing the expression of NO synthase and restoring MMP-9/TIMP-1 balance (Liu, 2011)
HCF were also shown to express delayed rectifier IK, Ito, Ca2+-activated K+ current (BKCa), inward-rectifier (Kir-type), and swelling-induced Cl- current (ICl.vol) channels (Yue, 2013).Cell Line OriginHeartApplicationhuman heart function, neointimal formation, vascular remodeling, myocardial repair, extracellular maintenance, signaling, toxicology, gene expression, structural support for cardiomycytes, synthesis of extracellular matrix, growth factors and cytokinesComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 1st passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 8 doublings
Subculture RoutinePlease refer to the HCF Culture Protocol.Параметрыbiological source human heart (normal adult) Quality Level 100 packaging pkg of 500,000 cells growth mode adherent karyotype 2n = 46 morphology fibroblast application(s) cell culture | mammalian: suitable relevant disease(s) cardiovascular diseases shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
Human Cardiac Fibroblasts: HCF, fetal 306-05F
NACRES: NA.81 General descriptionCardiac fibroblasts are the most prevalent cell type in the heart, making up 60-70 % of all cells. HCF from Cell Applications, Inc. provide an excellent model system to study many aspects of human heart function and pathophysiology.
HCF has been utilized in a number of research publications, for example to:- Determine that electrical coupling between cardiomyocytes and fibroblasts is mediated by large-conductance Ca2+-activated K+ channels that can be stimulated by estrogen receptor agonists (Wang, 2006, 2007); and show that antimitogenic effects of estradiol on HCF growth are mediated by cytochromes 1A1/1B1-and catechol-O-methyltransferase-derived metabolites (Dubey, 2005)
- Show that in response to mechanical stretch, cardiac fibroblasts release TGF- which causes trombomodulin downregulation, increasing the risk of thromboembolic events (Kapur, 2007) and also induces cardiac fibroblast differentiation into myofibroblasts via increased Smad2 and ERK1/2 phosphorylation, that could be stimulated by endothelin-1 and inhibited by Ac-SDKP (Peng, 2010)
- Demonstrate that activation of G protein-coupled receptor kinase-2 (GRK2) prevents normal regulation of collagen synthesis in cardiac fibroblasts mimicking heart failure phenotype (DSouza, 2011); identify FGF2 signaling pathway as potential target for modulating apoptosis in cardiac pathology (Ma, 2011) and investigate the roles of scleraxis (Bagchi, 2012) and AMPK1 (Noppe, 2014) in scar formation following myocardial infarction
- Show that the KATP channel opener KMUP-3 preserved cardiac function after myocardial infarction by enhancing the expression of NO synthase and restoring MMP-9/TIMP-1 balance (Liu, 2011)
HCF were also shown to express delayed rectifier IK, Ito, Ca2+-activated K+ current (BKCa), inward-rectifier (Kir-type), and swelling-induced Cl- current (ICl.vol) channels (Yue, 2013).Cell Line OriginHeartApplicationhuman heart function, neointimal formation, vascular remodeling, myocardial repair, extracellular maintenance, signaling, toxicology, gene expression, structural support for cardiomycytes, synthesis of extracellular matrix, growth factors and cytokinesComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 1st passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 8 doublings
Subculture RoutinePlease refer to the HCF Culture Protocol.ПараметрыQuality Level 100 biological source human heart (normal) packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology fibroblast application(s) cell culture | mammalian: suitable relevant disease(s) cardiovascular diseases shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
Human Carotid Artery Endothelial Cells: HCtAEC, adult 3014-05A
General descriptionHCtAEC has been used to elucidate molecular mechanisms of cerebral aneurism caused by haemodynamic (sheer) stress and inflammation, which were shown to act through PGE2-EP2 and NF-B signaling (Aoki, 2011). Demonstrating a link between inflammation and neovascularization, serum amyloid A, a biomarker of inflammation, increased expression of TNF, F3 factor, NF-B and VEGF leading to activated migration, wound healing and tube formation responses in HCtAEC; these pro-angiogenic activities could be prevented by pretreatment with the multi-angiokinase receptor inhibitor BIBF1120 (Cai, 2013). Additionally, CD40-CD154 signaling between endothelial cells and T cells leads to a stress response in endothelial cells via activation of NF-B and MAPK/SAPK pathways and induces down-regulation of APLN, while at the same time activating viral immune surveillance system involving TLR3, IFIH1, RIG-I, and RNASEL (Pluvinet, 2008).
Also, HCtAEC, along with human aortic (HAOEC), brachiocephalic artery (HBcAEC), coronary artery (HCAEC) and subclavian artery (HScAEC) have been used to demonstrate that not only blood vessels from different tissues are highly heterogeneous, they also interact differently with leukocytes during the inflammation response (Scott, 2013). The authors further showed that differential N-glycosylation of commonly expressed vascular adhesion molecules may be responsible for this heterogeneity, as well as for modulation of signaling under resting and activated inflammatory conditions. This also explains why specific vascular beds may be more or less susceptible to particular diseases or stimuli. Importantly, if cells from different sources were used, these results could not be convincingly validated due to a number of uncontrolled variables, such as age, race, genetic variability or life style choices of the donors.
Because of the complex heterogeneity that exists not only between different donors, but even between different vascular beds in the same individual, it would be prudent to confirm any new findings on primary cell lots coming from several different origins.Cell Line OriginArteryApplicationneovascularization, cell migration, wound healing, tube formation, angiogenesis, signal tranduction, monocyte chemotactic factor production, cell adhesionComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 10 doublings
Subculture RoutinePlease refer to the HCtAEC Culture Protocol.ПараметрыQuality Level 100 biological source human carotid artery (normal) packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology Endothelial application(s) cell culture | mammalian: suitable relevant disease(s) cardiovascular diseases shipped in dry ice storage temp. −196°C
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Human Carotid Artery Smooth Muscle Cells: HCtASMC, adult 3514-05A
NACRES: NA.81 General descriptionHuman Carotid Artery Smooth Muscle Cells (HCtASMC) provide a useful in vitro system to study all aspects of cardiovascular function and disease, especially those related to mechanisms of hyperplasia and hypertrophy of intimal smooth muscle cells leading to vascular occlusion in atherosclerosis and stent restenosis.Characterization: positive for smooth muscle cell specific alpha-actin expression.Cell Line OriginArteryApplicationcardiovascluar function, transfer of oxygenated blood to skull, brain, neck, face, effects of drugsComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 10 doublings
Subculture RoutinePlease refer to the HCtASMC Culture Protocol.ПараметрыQuality Level 100 biological source human carotid artery (normal) packaging pkg of 500,000 cells growth mode Adherent karyotype 23 application(s) cell culture | mammalian: suitable relevant disease(s) stroke; cardiovascular diseases storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
Human Chondrocytes - Osteoarthritis: HC-OA, adult 402OA-05A
NACRES: NA.81 General descriptionHC-OA Human Chondrocytes are derived from human articular cartilage of donors with osteoarthritis (OA). Osteoarthritis is an inflammatory disease characterized by increased degradation of cartilage tissue in the joint due to overproduction of enzymes degrading the extracellular matrix. Despite the initial proliferation and activation of chondrocytes, they are not able to efficiently repair the degrading cartilage. Instead, chondrocytes undergo terminal differentiation and eventually apoptose, leading to mineralization of cartilage in a process resembling bone formation during development. Thus, HC-OA provide a useful model to study changes in chondrocyte biology in response to abnormal environment of the OA joint.
Characterization: Positive for aggrecan after differentiation.
HC-OA have been used to demonstrate that THF-alpha activates transcription of ADAMTS-4, a metalloproteinase implicated in cartilage degradation in OA, via p38-MAPK-dependent mechanism (Xue, 2013).Cell Line OriginCartilageApplicationmodel for studying chondrocyte biology in an osteoarthritis jointComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 1st passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 10 doublings
Subculture RoutinePlease refer to the HC-OA Culture Protocol.Параметрыbiological source human articular cartilage (osteoarthritic) Quality Level 100 packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology chondrocyte application(s) cell culture | mammalian: suitable relevant disease(s) arthritis shipped in dry ice storage temp. −196°C
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Human Chondrocytes - Rheumatoid Arthritis: HC-RA, adult 402RA-05A
NACRES: NA.81 General descriptionHC-RA Human Chondrocytes are derived from human articular cartilage of donors with rheumatoid arthritis (RA). Rheumatoid arthritis is an inflammatory disease that is characterized by overproliferation of synovial cells and their infiltration into adjacent joint tissues, accompanied by gradual destruction of cartilage and bone erosion. Normally, chondrocytes are responsible for producing and maintaining the extracellular matrix of cartilage, but in RA cartilage, the environment is changed significantly due to inflammation and the invasion of other cells. Therefore, HC-RA can be a useful model to study the effects of those changes on chondrocyte biology. In parallel with normal HC, HC-RA can be used to investigate the differences between normal and pathological joints and evaluate potential treatment strategies.
Characterization: Positive for aggrecan after differentiation.Cell Line OriginCartilageApplicationmodel to study effects of RA on chondrocytes, investigate differences between normal and pathological joints and evaluate potential treatment strategiesComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 1st passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 10 doublings
Subculture RoutinePlease refer to the HC-RA Culture Protocol.Параметрыbiological source human articular cartilage (rheumatoid arthritic) Quality Level 100 packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology chondrocyte application(s) cell culture | mammalian: suitable relevant disease(s) arthritis shipped in dry ice storage temp. −196°C
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Human Chondrocytes: HC, adult 402-05A
NACRES: NA.81 General descriptionHuman Chondrocytes, HC, are derived from normal human articular cartilage where they produce and maintain the extracellular matrix of cartilage, including type II collagen. Chondrocytes grown in monolayer culture on a solid surface tend to lose their phenotypic markers and became de-differentiated to a fibroblast-like phenotype. This de-differentiation stage can be reversed by culturing them in a semi-solid gel.
Normal Chondrocytes have been adapted as an in vitro model system in multiple studies looking for cellular mechanisms, such as inflammation-related signaling cascades, abnormal proteinase production, chondrocyte apoptosis and differentiation, as well as novel potential treatments for arthritic disease.
Characterization: Positive for aggrecan after differentiation.
Normal Human Chondrocytes (HC) have been used to:- Develop a high throughput assay to screen for genes capable of inducing an OA-like phenotype in chondrocytes in order to identify key pathways implicated in the disease (Daouti, 2005)
- Elucidate the signaling cascade leading to induction of proinflammatory cytokines by chondrocytes in RA joints (Aida, 2006; Wang, 2011a,b) and effects of elevated IL-6 signaling on normal chondrocytes (Namba, 2007)
- Investigate mechanisms and identify inhibitors of increased production of proteinases by chondrocytes stimulated by interleukin-1 (Aida, 2005; Wada, 2006), retinoic acid (Hikichi, 2009), proinflammatory cytokines (Tanigawa, 2011a,b), nitric oxide (Wu, 2007; Yang, 2011) and shear stress (Wang, 2011b, 2012)
- Investigate the causes of metalloproteinases induction in patients with Lyme disease-associated arthritis (Lin, 2001; Behera, 2004, 2005)
- Study the factors affecting chondrocyte apoptosis (Cherng, 2008; Malemud, 2012) and differentiation into osteoclasts (Watanabe, 2009a,b; Honda, 2011)
- Show that hyaluronate (HA) can prevent the aggravated cartilage degradation by blocking the matrix metalloproteinases production from cytokine-activated chondrocytes via MKP-1 induction through CD44 signaling (Hashizume, 2009, 2010)
- Demonstrate cytotoxic effects of anti-human Fas/APO-1/CD95 (Fas) monoclonal antibody ARG098 on RA synoviocytes and infiltrating lymphocytes, but not on normal chondrocytes (Tamburstuen, 2010)
Normal Chondrocytes were also utilized in a functional cluster formation agarose assay that allows chondrocytes to maintain their differentiated state (Quintavalla, 2005) and to investigate integrin-mediated mechanotransduction pathways required for proper chondrocyte function (Whitney, 2012). Additionally, they have used extensively in material studies aimed to improve chondrocyte adhesion to medical implants (Gutwein, 2002; Ellison, 2003; Price, 2004; Savaiano, 2004; Burns, 2009) and scaffolds for cartilage regeneration (Jun, 2002; Kay, 2002; Miller, 2002a,b; Price, 2002, 2003; Rao, 2004; Park, 2005; Khang, 2008). Normal chondrocyte RNA was used as a gold standard control in research on cellular reprogramming into chondrocytes (Ishii, 2012).Cell Line OriginCartilageApplicationproduction and maintenance of extracellular matrix, cartilage, collagen, differentiation and de-differentiation, signal transduction, apoptosis, differentiation, drug screening, gene expression, cytokine production, agarose assays, chondrocyte adhesion to medical implants, scaffolds for cartilage regenerationComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 1st passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 10 doublings
Subculture RoutinePlease refer to the HC Culture Protocol.ПараметрыQuality Level 100 biological source human articular cartilage packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology chondrocyte application(s) cell culture | mammalian: suitable relevant disease(s) arthritis shipped in dry ice storage temp. −196°C
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Human Chondrocytes: HC, fetal 402-05F
NACRES: NA.81 General descriptionHuman Chondrocytes, HC, are derived from normal human articular cartilage where they produce and maintain the extracellular matrix of cartilage, including type II collagen. Chondrocytes grown in monolayer culture on a solid surface tend to lose their phenotypic markers and became de-differentiated to a fibroblast-like phenotype. This de-differentiation stage can be reversed by culturing them in a semi-solid gel.
Normal Chondrocytes obtained from Cell Applications, Inc. have been adapted as an in vitro model system in multiple studies looking for cellular mechanisms, such as inflammation-related signaling cascades, abnormal proteinase production, chondrocyte apoptosis and differentiation, as well as novel potential treatments for arthritic disease.
Characterization: Positive for aggrecan after differentiation.
Normal Human Chondrocytes (HC) have been used to:- Develop a high throughput assay to screen for genes capable of inducing an OA-like phenotype in chondrocytes in order to identify key pathways implicated in the disease (Daouti, 2005)
- Elucidate the signaling cascade leading to induction of proinflammatory cytokines by chondrocytes in RA joints (Aida, 2006; Wang, 2011a,b) and effects of elevated IL-6 signaling on normal chondrocytes (Namba, 2007)
- Investigate mechanisms and identify inhibitors of increased production of proteinases by chondrocytes stimulated by interleukin-1 (Aida, 2005; Wada, 2006), retinoic acid (Hikichi, 2009), proinflammatory cytokines (Tanigawa, 2011a,b), nitric oxide (Wu, 2007; Yang, 2011) and shear stress (Wang, 2011b, 2012);
- Investigate the causes of metalloproteinases induction in patients with Lyme disease-associated arthritis (Lin, 2001; Behera, 2004, 2005)
- Study the factors affecting chondrocyte apoptosis (Cherng, 2008; Malemud, 2012) and differentiation into osteoclasts (Watanabe, 2009a,b; Honda, 2011)
- Show that hyaluronate (HA) can prevent the aggravated cartilage degradation by blocking the matrix metalloproteinases production from cytokine-activated chondrocytes via MKP-1 induction through CD44 signaling (Hashizume, 2009, 2010)
- Demonstrate cytotoxic effects of anti-human Fas/APO-1/CD95 (Fas) monoclonal antibody ARG098 on RA synoviocytes and infiltrating lymphocytes, but not on normal chondrocytes (Tamburstuen, 2010)
Normal Chondrocytes were also utilized in a functional cluster formation agarose assay that allows chondrocytes to maintain their differentiated state (Quintavalla, 2005) and to investigate integrin-mediated mechanotransduction pathways required for proper chondrocyte function (Whitney, 2012). Additionally, they have used extensively in material studies aimed to improve chondrocyte adhesion to medical implants (Gutwein, 2002; Ellison, 2003; Price, 2004; Savaiano, 2004; Burns, 2009) and scaffolds for cartilage regeneration (Jun, 2002; Kay, 2002; Miller, 2002a,b; Price, 2002, 2003; Rao, 2004; Park, 2005; Khang, 2008). Normal chondrocyte RNA was used as a gold standard control in research on cellular reprogramming into chondrocytes (Ishii, 2012).Cell Line OriginCartilageApplicationproduction and maintenance of extracellular matrix, cartilage, collagen, differentiation and de-differentiation, signal transduction, apoptosis, differentiation, drug screening, gene expression, cytokine production, agarose assays, chondrocyte adhesion to medical implants, scaffolds for cartilage regenerationComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 10 doublings
Subculture RoutinePlease refer to the HC Culture Protocol.ПараметрыQuality Level 100 biological source human articular cartilage packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology chondrocyte application(s) cell culture | mammalian: suitable relevant disease(s) arthritis shipped in dry ice storage temp. −196°C
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Human Coronary Artery Endothelial Cells: HCAEC, adult 300-05A
NACRES: NA.81 General descriptionHCAEC provides an excellent model system to study all aspects of cardiovascular function and disease, and they have been utilized in dozens of research publications, for example to:- Understand the mechanism of the anti-inflammatory properties of HDL, and demonstrate for the first time that mature miRNA can control gene expression in a cell where it is neither transcribed nor processed (Tabet, 2014)
- Study mechanisms of angiogenesis, as well as oxidative stress and inflammation related pathways in endothelia (Ji, 2009; Wang, 2011; Quinn, 2011; Hung, 2010; Rajesh, 2010; Riegel, 2011; Lin, 2013; Lloid, 2013Baley-Downs, 2012; Kapur, 2012; Melchior, 2012; Castanares-Zapatero, 2013; Hankins, 2013; Lord, 2013; dela Paz, 2013; Takai, 2013), including gender and race specific differences in patients with peripheral artery disease (Gardner, 2014)
- Elucidate molecular mechanisms of various cardiovascular risk factors, including those associated with diabetes (Vladik, 2011; Kapur, 2011; Dunn, 2013; Leucker, 2013; Liu, 2013, 2014; Morgan, 2014; Torella, 2014)
- Understand the mode of action and cardiovascular protection effects of various natural compounds, vitamins and drug candidates (Candelario, 2013; Ramirez-Sanchez, 2010, 2013; Lee, 2013; Nsimba, 2013; Di Bartolo, 2011; Baotic, 2013; Murphy, 2013; Tan, 2013; Wu, 2012), as well as caloric restriction (Csiszar, 2009, 2013)
- Develop and evaluate scaffolds and hydrogels for cardiac tissue engineering (Singelyn, 2009, 2011; Seif-Naraghi, 2010; Johnson, 2014), and new treatment strategies to prevent stent restenosis (ONeill, 2009; OBrien, 2010; Crowder, 2011, 2012; Eppihimer, 2013; Hiob, 2013)
- Compare effects of BMP-4 on HCAEC and Human Pulmonary Artery Endothelial Cells (HPAEC) and show that only in HCAEC BMP-4 treatment induced ROS, activated NF-kB, ICAM-1 and increased monocyte adhesiveness, explaining why its upregulation leads to atherosclerosis and hypertension in the systemic, but not pulmonary circulation (Csiszar, 2008)
Additionally, HCAEC, along with human aortic (HAOEC), carotid artery (HCtAEC), subclavian artery (HScAEC) and brachiocephalic artery (HBcAEC) have been used to demonstrate that not only blood vessels from different tissues are highly heterogeneous, they also interact differently with leukocytes during the inflammation response (Scott, 2013). The authors further showed that differential N-glycosylation of commonly expressed vascular adhesion molecules may be responsible for this heterogeneity, as well as for modulation of signaling under resting and activated inflammatory conditions. This also explains why specific vascular beds may be more or less susceptible to particular diseases or stimuli. Importantly, if cells from different sources were used, these results could not be convincingly validated due to a number of uncontrolled variables, such as age, race, genetic variability or life style choices of the donors.
Because of the complex heterogeneity that exists not only between different donors, but even between different vascular beds in the same individual, it would be prudent to confirm any new findings on primary cell lots coming from several different origins.Cell Line OriginArteryApplicationcardiovascular function, angiogenesis, vascular biology, regulation of coronary blood flow, cardiac functions, effects of vaso-dilators, coagulation, fibrinolysis, vessel wall tone, cytokine productionComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 15 doublings
Subculture RoutinePlease refer to the HCAEC Culture Protocol,.ПараметрыQuality Level 100, biological source human coronary artery (normal) packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology Endothelial application(s) cell culture | mammalian: suitable relevant disease(s) cardiovascular diseases shipped in dry ice storage temp. −196°C
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Human Coronary Artery Smooth Muscle Cells: HCASMC, adult 350-05A
NACRES: NA.81 General descriptionHuman Coronary Artery Smooth Muscle Cells (HCASMC) provide an excellent model system to study all aspects of cardiovascular function and disease, especially those related to mechanisms of hyperplasia and hypertrophy of intimal smooth muscle cells leading to vascular occlusion in atherosclerosis and stent restenosis.
HCASMC has been utilized in a number of research studies, for example, to:- Study signaling pathways regulating smooth muscle differentiation (Zhou, 2010); and chronic inflammation of arterial wall that leads to artherosclerosis (Kiyan, 2014)
- Demonstrate that STAT-1 and STAT-3 regulate VEGF production in smooth muscle cells by having opposing effects on HIF-1 expression (Albasanz-Puig, 2012); study the mechanisms of hypoxia and reoxigenation injuries in by demonstrating increased production of ROS and inflammatory cytokines, and further showing that DHA is not beneficial in this type of injuries (Feng, 2012)
- Investigate the gene expression differences between smooth muscle cells from different arteries, underlying their differential response to injuries and proliferation stimuli (Lange, 2013)
- Suggest the hypermethylation of SOCS3 gene as the connection between TNF- and IGF-1 released in response to mechanical injury during coronary intervention, and the induction of cytokines leading to intimal hyperplasia and restenosis (Dhar, 2013)
- Develop a novel VEGFR/MET-targeted inhibitor with improved antitumor efficacy and decreased toxicity (Fujita, 2013); and investigate novel therapies and drug combinations to achieve optimal target selectivity (Lehar, 2009; Wo-Wong, 2013)
- Develop elastic scaffolds for tissue engineering (Nivison-Smith, 2010, 2012) and novel treatment strategies to prevent stent restenosis by designing new materials (Crowder, 2012), or drug therapies to preferentially inhibit smooth muscle cell growth (ONeill, 2009; Mociornita, 2013)
Characterization: positive for smooth muscle cell specific alpha-actin expression.Cell Line OriginArteryApplicationvascular research, supply of blood to heart muscleComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 16 doublings
Subculture RoutinePlease refer to the HCASMC Culture Protocol,.Параметрыbiological source human coronary artery (normal, tunica intima and media) Quality Level 100, packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology smooth muscle application(s) cell culture | mammalian: suitable relevant disease(s) diabetes; stroke; cardiovascular diseases shipped in dry ice storage temp. −196°C
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Human Dermal Fibroblasts: HDF, adult 106-05A
NACRES: NA.81 General descriptionDermal Fibroblasts are responsible for producing the extracellular matrix forming the connective tissue of the skin, and play a crucial role during wound healing. HDF provide an excellent model system to study many aspects of cell physiology, and have been utilized in dozens of research publications, particularly those related to skin biology and reprogramming/induced pluripotency studies. HDF were used to create, along with human synovyocytes, induced pluripotent stem cells (iPSC) by using the now classic “Yamanaka cocktail” of Oct3/4, Sox2, Klf4, and c-Myc, the discovery for which Dr. Shinya Yamanaka was awarded the Nobel Prize in 2012 (Takanashi, 2007; 2009), and to characterize iPSC general properties, miRNA expression profiles and differentiation potential (Hayashi, 2010; Ohta, 2011; Koyanagi-Aoi, 2013; Noguchi, 20130; Razak, 2013; Sakurai, 2013; Tanaka, 2013; Yanagida, 2013)Cell Line OriginSkinApplicationinjury recovery, connective tissue research, laminin and fibronectin production, extracellular matrix, tissue organization, wound healing, fibroblasts growth factor responseComponentsFibroblast Basal Medium containing 10% FBS & 10%Preparation Note- Primary culture, >500,000 cells in Fibroblast Basal Medium containing 10% FBS & 10%
- DMSOCan be cultured at least 16 doublings
Culture MediumFibroblast Growth Medium (116-500), all-in-one ready to use mediaSubculture RoutinePlease refer to the HDF Culture Protocol,.ПараметрыQuality Level 100, biological source human neonatal foreskin or adult skin (normal) packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology Fibroblast application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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Human Dermal Fibroblasts: HDF, fetal 106-05F
NACRES: NA.81 General descriptionDermal Fibroblasts are responsible for producing the extracellular matrix forming the connective tissue of the skin, and play a crucial role during wound healing. HDF provide an excellent model system to study many aspects of cell physiology, and have been utilized in dozens of research publications, particularly those related to skin biology and reprogramming/induced pluripotency studies. HDF were used to create, along with human synovyocytes, induced pluripotent stem cells (iPSC) by using the now classic “Yamanaka cocktail” of Oct3/4, Sox2, Klf4, and c-Myc, the discovery for which Dr. Shinya Yamanaka was awarded the Nobel Prize in 2012 (Takanashi, 2007; 2009), and to characterize iPSC general properties, miRNA expression profiles and differentiation potential (Hayashi, 2010; Ohta, 2011; Koyanagi-Aoi, 2013; Noguchi, 20130; Razak, 2013; Sakurai, 2013; Tanaka, 2013; Yanagida, 2013)Cell Line OriginSkinApplicationinjury recovery, connective tissue research, laminin and fibronectin production, extracellular matrix, tissue organization, wound healing, fibroblasts growth factor responseComponentsFibroblast Basal Medium containing 10% FBS & 10% DMSOPreparation Note- Primary culture, >500,000 cells in Fibroblast Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 16 doublings
Subculture RoutinePlease refer to the HDF Culture Protocol,.ПараметрыQuality Level 100, biological source human neonatal foreskin or adult skin (normal) packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology Fibroblast application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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Human Dermal Fibroblasts: HDF, neonatal 106-05N
NACRES: NA.81 General descriptionDermal Fibroblasts are responsible for producing the extracellular matrix forming the connective tissue of the skin, and play a crucial role during wound healing. HDF provide an excellent model system to study many aspects of cell physiology, and have been utilized in dozens of research publications, particularly those related to skin biology and reprogramming/induced pluripotency studies. HDF were used to create, along with human synovyocytes, induced pluripotent stem cells (iPSC) by using the now classic “Yamanaka cocktail” of Oct3/4, Sox2, Klf4, and c-Myc, the discovery for which Dr. Shinya Yamanaka was awarded the Nobel Prize in 2012 (Takanashi, 2007; 2009), and to characterize iPSC general properties, miRNA expression profiles and differentiation potential (Hayashi, 2010; Ohta, 2011; Koyanagi-Aoi, 2013; Noguchi, 20130; Razak, 2013; Sakurai, 2013; Tanaka, 2013; Yanagida, 2013)Cell Line OriginSkinApplicationinjury recovery, connective tissue research, laminin and fibronectin production, extracellular matrix, tissue organization, wound healing, fibroblasts growth factor responseComponentsFibroblast Basal Medium containing 10% FBS & 10% DMSOPreparation Note- Primary culture, >500,000 cells in Fibroblast Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 16 doublings
Subculture RoutinePlease refer to the HDF Culture Protocol,.ПараметрыQuality Level 100, biological source human neonatal foreskin or adult skin (normal) packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology Fibroblast application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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Human Dermal Microvascular Endothelial Cells: CADMEC/HMVEC, adult 100-05A
NACRES: NA.81 General descriptionCADMEC/HMVEC provide an excellent model system to study many aspects of endothelial function and disease, especially those related to the microvasculature and capillary systems. HMVEC/CADMEC have been shown to express vWF, CD36 and CD31, are able to uptake DiI-Ac-LDL and are positive for functional activity by cytokine-stimulated leukocyte adherence.
Select HMVEC lots have been additionally tested to demonstrate stimulation-dependent angiogenesis and key endothelial cell signaling pathways (phosphorylation of VEGFR, Akt, MAPK, and expression of Tie2, eNOS, Axl and Etk/Bmx).Cell Line OriginSkinApplicationEndothelial vessel function, cell signaling, angiogenesisComponentsCell Basal Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Cell Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 16 doublings
Subculture RoutinePlease refer to the CADMEC/HMVEC Culture Protocol,.Параметрыbiological source human neonatal foreskin or adult skin capillaries (normal) Quality Level 100, packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology Endothelial application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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Human Dermal Microvascular Endothelial Cells: CADMEC/HMVEC, neonatal 100-05N
NACRES: NA.81 General descriptionCADMEC/HMVEC provide an excellent model system to study many aspects of endothelial function and disease, especially those related to the microvasculature and capillary systems. HMVEC/CADMEC have been shown to express vWF, CD36 and CD31, are able to uptake DiI-Ac-LDL and are positive for functional activity by cytokine-stimulated leukocyte adherence.
Select HMVEC lots have been additionally tested to demonstrate stimulation-dependent angiogenesis and key endothelial cell signaling pathways (phosphorylation of VEGFR, Akt, MAPK, and expression of Tie2, eNOS, Axl and Etk/Bmx).Cell Line OriginSkinApplicationEndothelial vessel function, cell signaling, angiogenesisComponentsCell Basal Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Cell Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 16 doublings
Subculture RoutinePlease refer to the CADMEC/HMVEC Culture Protocol,.Параметрыbiological source human neonatal foreskin or adult skin capillaries (normal) Quality Level 100, packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology Endothelial application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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Human Dermal Microvascular Endothelial Cells: CADMEC/HMVEC: Pre-Screened for Angiogenesis & VEGF signaling, adult S100-05A
NACRES: NA.81 General descriptionEndothelial cells (EC) line the blood vessels of the entire circulatory system, from aorta to capillaries, and act as a barrier between circulating blood and the rest of the vessel wall/surrounding tissues. Endothelial cells are involved in the processes of angiogenesis, vasculogenesis, vasoregulation, coagulation, fibrinolysis and selective blood barrier. They are also implicated in pathophysiological processes, including cardiovascular disease and cancer development.
The most commonly used human endothelial cells are Human Umbilical Vein Endothelial Cells, (HUVEC), Human Microvascular Endothelial Cells (HMVEC) and Human Aortic Endothelial Cells (HAOEC). Select lots of HUVEC, HMVEC, and HAOEC have been pre-screened to demonstrate stimulation-dependent angiogenesis and key endothelial cell signaling pathways (phosphorylation of VEGFR, Akt, MAPK, and expression of Tie2, eNOS, Axl and Etk/Bmx.
VEGF-Stimulated Signaling in Pre-Screened Endothelial Cells
VEGF receptor-2 (VEGFR-2) is a major VEGF of endothelial cells. VEGFR-2-mediated signaling plays a critical role in angiogenesis, including regulation of proliferation, differentiation, cell movement, and survival of endothelial cells. VEGF-induced receptor dimerization triggers activation of VEGFR-2 tyrosine kinase and autophosphorylation at a specific set of tyrosine residues, which serve as docking sites for downstream signaling components leading to activation of downstream signaling molecules, including phosphorylation of Akt and p44/42-MAPK.
Expression of Signaling Biomarkers in Pre-Screened Endothelial Cells
Prescreened Endothelial Cells express the following important biomarkers:- Tie2, a receptor tyrosine kinase critical for the angiogenic remodeling, sprout formation, survival of endothelial cells and vessel stabilization processes;
- eNOS (endothelial Nitric Oxide Synthase), the enzyme that producse NO which is an important signaling molecule that regulates a diverse range of physiological events and is required for normal endothelial function;
- Axl, a receptor tyrosine kinase whose ligand is the survival factor Gas6 (growth arrest-specific gene 6 product) implicated in many processes, such as cell survival, leukocyte transmigration and neointima formation.
- Etk/Bmx (Endothelial/epithelial Tyrosine Kinase), a member of the Btk family, participates in signal transduction stimulated by growth factor receptors, cytokine receptors, G-protein-coupled receptors, antigen receptors, and integrins, and has been implicated in cell adhesion, migration, proliferation, and survival.
Cell Line OriginBlood VesselsApplicationEndothelial vessel function, cell signaling, angiogenesisComponentsCell Basal Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Cell Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 16 doublings
Subculture RoutinePlease refer to the CADMEC/HMVEC Culture Protocol,.Параметрыbiological source human blood vessels packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology Endothelial application(s) cell culture | mammalian: suitable storage temp. −196°C
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Менеджер по работе с клиентами
С 2010 года мы поставляем оборудование с заводов Европы. Берем на себя все — от подбора оборудования до внедрения на предприятии
Все сотрудники имеют высшее образование, закончили ведущие химические вузы страны, такие как РХТУ им Менделеева.
У большинства компаний срок ожидания составляет 10-12 недель.
Оборудование хранится на сухом отапливаемом складе, где поддерживается ровная температура.
Работаем с PonyExpress и Деловыми линиями. Вы также можете выбрать свою транспортную компанию или забрать товар со склада в Москве.
В случае любых неполадок за свой счет выполним ремонт в сервисном центре или на заводе-изготовителе. Или бесплатно заменим прибор на новый.
Производим пуско-наладку оборудования, валидацию, обучение сотрудников. Если нужно, привлекаем инженеров с заводов- изготовителей.
